Correlation between Polymorphism and Productive Traits of Texel × Kazakh Sheep Analyzed Using Microsatellite Markers
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摘要:
目的 利用微卫星标记分析特克塞尔×哈萨克羊群体的遗传多样性，并与生产性状进行关联分析，为该品种的遗传育种和性状改良提供分子标记。 方法 选取11个微卫星标记，采用PCR扩增和毛细管电泳技术检测108只特克塞尔×哈萨克羊杂交群体的遗传多样性，并对各位点不同基因型与生产性状进行关联分析。 结果 11个标记位点在该群体中共检测到91个等位基因，平均等位基因数为8.273，平均观测杂合度为0.428，平均期望杂合度为0.815，平均多态信息含量为0.789。关联分析发现如下位点与相应性状之间呈极显著或显著相关：AMEL、ETH152、INRA006和INRA023与初生重、断奶重、宰前重和胴体重；CSRD247与胴体长、腿垂直长；INRA005、MAF214和MCM527与腿会斜长、腿耻斜长和踝骨宽；INRA172和OARFCB20位点与臀宽、胸宽和肩宽；INRA063与胸深。 结论 11个微卫星标记位点在该群体中具有较丰富的遗传多态性，且与生产性状均有不同程度的关联性，可以为特克塞尔×哈萨克羊性状改良和育种工作提供分子标记。 Abstract:Objective Microsatellite markers were used to analyze the correlation between the genetic polymorphisms and productive traits of Texel×Kazakh sheep for breed improvement. Method Eleven microsatellite marker sites were selected for the study. Genetic polymorphisms of 108 sheep were determined using PCR and capillary electrophoresis. Correlation between the genotypes and productive traits of the sheep at each site was analyzed. Result A total of 91 alleles were detected at 11 microsatellite loci with an averaged number of alleles at 8.273, an observed heterozygosity at 0.428, an expected heterozygosity at 0.815, and a content of polymorphic information at 0.789. The following loci were found extremely significantly or significantly correlate with their corresponding traits: AMEL, ETH152, INRA006 and INRA023 loci with primary weight, weaning weight, pre-mortem weight, and weight of the salamander; CSRD247 locus with lengths of carcass and legs; INRA005, MAF214 and MCM527 with leg will be obliquely long, the legs are obliquely long and the ankle width; INRA172 and OARFCB20 with widths of hip, chest and shoulder; and, INRA063 with chest depth. Conclusion The 11 selected microsatellite markers were rich in genetic polymorphisms among the sheep population. They all correlated in different degrees with the various productive traits of a sheep suggesting a potential tool in breeding of Texel×Kazakh sheep with increased meat producing capacity. -
Key words:
- Texel×Kazakh sheep /
- microsatellite markers /
- genetic polymorphism /
- productive traits
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表 1 微卫星标记引物序列
Table 1. Microsatellite-labeled primer sequences
位点
Loci引物序列
Primer sequence（5′→3′）退火温度
Annealing temperature/℃片段长度
Product length/bpAMEL F:CAGCCAAACCTCCCTCTGC 58 X and Y R:CCCGCTTGGTCTTGTCTGTTGC CSRD247 F:GGACTTGCCAGAACTCTGCAAT 58 209–255 R:CACTGTGGTTTGTATTAGTCAGG ETH152 F:TACTCGTAGGGCAGGCTGCCTG 56 186–200 R:GAGACCTCAGGGTTGGTGATCAG INRA005 F:TTCAGGCATACCCTACACCACATG 56 125–147 R:AAATATTAGCCAACTGAAAACTGGG INRA006 F:AGGAATATCTGTATCAACCGCAGTC 58 110–132 R:CTGAGCTGGGGTGGGAGCTATAAATA INRA023 F:GAGTAGAGCTACAAGATAAACTTC 52 194–216 R:TAACTACAGGGTGTTAGATGAACTC INRA063 F:GACCACAAAGGGATTTGCACAAGC 58 169–201 R:AAACCACAGAAATGCTTGGAAG INRA172 F:CCAGGGCAGTAAAATGCATAACTG 58 126–160 R:GGCCTTGCTAGCCTCTGCAAAC MAF214 F:AATGCAGGAGATCTGAGGCAGGGACG 58 189–265 R:GGGTGATCTTAGGGAGGTTTTGGAGG MCM527 F:GTCCATTGCCTCAAATCAATTC 58 164–170 R:AAACCACTTGACTACTCCCCAA OARFCB20 F:GGAAAACCCCCATATATACCTATAC 56 87–113 R:AAATGTGTTTAAGATTCCATACATGTG 
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表 2 11个微卫星位点的遗传多态性信息
Table 2. Genetic polymorphisms of 11 microsatellite loci
微卫星
位点
Loci群体
个体数
N等位
基因数
K观测
杂合度
Ho期望
杂合度
He多态信息
含量
PICAMEL 108 10 0.472 0.841 0.819 CSRD247 108 11 0.380 0.726 0.706 ETH152 108 5 0.333 0.760 0.718 INRA005 108 8 0.361 0.840 0.816 INRA006 108 8 0.389 0.821 0.795 INRA023 108 8 0.417 0.825 0.798 INRA063 108 10 0.500 0.870 0.851 INRA172 108 7 0.278 0.828 0.802 MAF214 108 10 0.630 0.866 0.847 MCM527 108 5 0.333 0.773 0.731 OARFCB20 108 9 0.611 0.820 0.794
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表 3 位点AMEL基因型与生产性状差异
Table 3. Differences on genotypes and productive traits at AMEL loci
基因型 Genotypes CI DI DJ GG HH 个体数 Individuals 8 7 7 18 25 初生重 Primary weight/kg 5.20±0.20 a 4.43±0.22 b 4.93±0.22 ab 4.78±0.13 ab 4.78±0.11 ab 断奶重 Weaning weight/kg 28.38±1.85 ABab 33.67±1.98 Aa 26.70±1.98 ABb 26.30±1.23 Bb 26.60±1.05 Bb 宰前重 Before slaughter /kg 37.46±2.15 B 45.80±2.30 A 34.68±2.30 B 37.51±1.43 B 37.23±1.22 B 胴体重 Carcass weight/kg 19.54±1.22 B 24.03±1.30 A 18.29±1.30 B 19.74±0.81 B 19.42±0.69 B 胴体长 Carcass length/cm 65.00±1.85 AB 67.29±1.98 A 63.43±1.98 AB 62.83±1.24 AB 61.24±1.05 B 腿垂直长 Legs vertical length/cm 21.38±0.85 ab 23.57±0.90 a 21.71±0.90 ab 21.39±0.56 b 21.32±0.48 b 腿会斜长 Leg will be obliquely long/cm 23.13±0.63 b 25.14±0.67 a 24.29±0.67 ab 23.22±0.42 b 23.88±0.35 ab 腿耻斜长 Legs are obliquely long/cm 31.00±0.97 AB 29.00±1.04 B 31.57±1.04 AB 31.11±0.65 AB 32.36±0.55 A 臀宽 Hip width/cm 21.13±0.84 ab 22.00±0.90 a 18.86±0.90 b 20.50±0.56 ab 20.48±0.47 ab 肩宽 Shoulder width/cm 19.63±0.99 ab 21.86±1.06 a 18.86±1.06 b 19.56±0.66 ab 19.56±0.56 ab 胸深 Chest depth/cm 25.50±0.79 ABa 26.43±0.85 Aa 24.29±0.85 ABab 23.56±0.53 Bb 23.28±0.45 Bb 注：同行数据后不同大写字母表示极显著差异（P＜0.01），不同小写字母表示显著差异（P＜0.05），相同字母表示差异无显著性（P＞0.05）。表4~13同。
Note: Data with different capital letters on same column indicate extremely significant difference（P＜0.01）; data with different lowercase letters indicate significant difference（P＜0.05）; and, same letters indicate no significant difference（P＞0.05）. The same for Table 4–13.
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表 4 位点INRA023基因型与生产性状差异
Table 4. Differences on genotypes and productive traits at INRA023 loci
基因型 Genotypes AD AF BH CC CH EE EH GG 个体数
Individuals7 8 9 24 12 19 9 10 初生重
Primary weight/kg5.06±0.21 ABa 5.18±0.20 ABa 4.47±0.19 Bb 4.65±0.11 Bb 5.25±0.16 Aa 4.74±0.13 ABb 4.74±0.19 ABb 4.70±0.18 ABb 断奶重
Weaning weight/kg35.16±2.02 Aa 28.04±1.89 ABb 25.95±1.79 Bb 26.72±1.09 Bb 29.64±1.55 ABb 25.29±1.23 Bb 28.70±1.79 ABb 26.44±1.69 Bb 宰前重
Before slaughter/kg48.72±2.26 A 38.41±2.12 B 36.43±2.00 B 37.69±1.22 B 40.75±1.73 B 35.77±1.37 B 38.27±2.00 B 36.09±1.89 B 胴体重
Carcass weight /kg26.36±1.30 A 20.13±1.22 B 19.15±1.15 B 19.67±0.70 B 21.24±0.99 B 18.62±0.79 B 20.07±1.15 B 18.99±1.09 B 胴体长
Back length /cm69.14±1.96 Aa 59.63±1.83 Bb 62.33±1.73 ABb 63.67±1.06 ABb 65.58±1.50 ABab 62.42±1.19 ABb 63.11±1.73 ABb 63.30±1.64 ABb 腿垂直长
Legs vertical length/cm24.00±1.01 a 21.25±0.95 b 21.11±0.89 b 21.13±0.55 b 22.00±0.77 ab 21.42±0.62 b 22.56±0.89 ab 22.10±0.85 ab 腿会斜长
Leg will be obliquely long/cm25.00±0.70 a 23.75±0.65 ab 23.11±0.62 b 23.42±0.38 b 23.42±0.53 ab 23.37±0.42 b 23.89±0.62 ab 24.80±0.58 a 踝骨宽
Ankle width/cm7.00±0.23 a 6.25±0.22 b 6.44±0.20 ab 6.42±0.13 b 6.25±0.18 b 6.32±0.14 b 6.33±0.20 b 6.40±0.19 ab 臀宽
Hip width /cm22.71±0.90 a 20.38±0.84 ab 20.00±0.79 b 20.29±0.49 b 21.25±0.69 ab 20.11±0.55 b 20.67±0.79 ab 20.10±0.75 b 胸宽
Chest width/cm27.86±1.10 Aa 24.00±1.03 ABb 23.33±0.97 Bb 24.17±0.59 ABb 25.33±0.84 ABab 22.58±0.67 Bb 23.78±0.97 Bb 23.00±0.92 Bb 肩宽
Shoulder width/cm21.71±1.05 a 20.25±0.98 ab 19.11±0.93 ab 19.79±0.57 ab 20.92±0.80 ab 19.16±0.64 b 19.22±0.93 ab 19.70±0.88 ab
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表 5 位点INRA172基因型与生产性状差异
Table 5. Difference on genotypes and productive traits at INRA172 loci
基因型 Genotypes AA BB DD FF GG 个体数 Individuals 12 13 8 11 26 断奶重 Weaning weight /kg 25.69±1.65 ab 30.13±1.58 a 27.94±2.02 ab 26.73±1.72 ab 25.50±1.12 b 宰前重 Before slaughter/kg 37.09±1.88 ab 41.04±1.80 a 37.23±2.30 ab 36.29±1.96 ab 35.94±1.28 b 胴体重 Carcass weight /kg 19.33±1.09 ab 21.42±1.05 a 19.74±1.34 ab 17.91±1.14 b 18.82±0.74 b 腿会斜长 Leg will be obliquely long/cm 23.58±0.56 ab 24.85±0.54 a 24.13±0.68 ab 23.73±0.58 ab 23.27±0.38 b 腿耻斜长 Legs are obliquely long/cm 31.50±0.86 ab 32.46±0.83 a 29.75±1.05 b 31.36±0.90 ab 31.12±0.58 ab 臀宽 Hip width/cm 21.08±0.70 a 20.77±0.67 ab 21.38±0.86 a 19.27±0.73 ab 19.23±0.48 b 胸宽 Chest width/cm 25.08±0.91 a 24.23±0.87 ab 23.13±1.11 ab 22.55±0.95 ab 22.42±0.62 b 肩宽 Shoulder width/cm 20.67±0.84 a 20.69±0.81 a 20.00±1.03 ab 18.91±0.88 ab 18.65±0.57 b
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表 6 位点CSRD247基因型与生产性状差异
Table 6. Differences on genotypes and productive traits at CSRD247 loci
基因型 Genotypes AF BF EE FF FJ 个体数 Individuals 6 6 7 45 6 胴体长 Carcass length/cm 67.33±2.25 a 65.00±2.25 ab 60.86±2.08 b 62.89±0.82 ab 62.00±2.25 ab 腿垂直长 Legs vertical length/cm 22.00±1.05 ab 24.00±1.05 a 20.71±0.98 b 21.18±0.39 b 22.67±1.05 ab 踝骨宽 Ankle width/cm 6.83±0.25 Aa 6.50±0.25 ABa 6.71±0.23 Aa 6.38±0.09 ABa 5.67±0.25 Bb
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表 7 位点ETH152基因型与生产性状差异
Table 7. Differences on genotypes and productive traits at ETH152 loci
基因型 Genotypes AC AE BB CC CE DD EE 个体数 Individuals 9 8 11 19 19 15 21 断奶重 Weaning weight/kg 30.73±1.90 a 28.43±2.01 ab 26.61±1.72 ab 28.90±1.31 ab 26.41±1.31 ab 25.47±1.47 b 26.32±1.24 ab 胴体重 Carcass weight/kg 21.18±1.32 ab 20.03±1.40 ab 19.32±1.19 ab 20.93±0.91 a 19.91±0.91 ab 19.12±1.02 ab 18.36±0.86 b 腿耻斜长 Legs are obliquely long/cm 31.11±0.95 ab 29.50±1.01 b 31.18±0.86 ab 32.16±0.65 a 30.63±0.65 ab 30.73±0.74 ab 31.86±0.62 a 踝骨宽 Ankle width/cm 6.89±0.20 Aa 6.75±0.21 ABa 6.09±0.18 Bb 6.42±0.14 ABab 6.32±0.14 ABb 6.27±0.16 ABb 6.24±0.13 Bb 胸宽 Chest width/cm 24.33±1.05 ab 25.50±1.11 a 22.82±0.95 ab 24.26±0.72 ab 24.21±0.72 ab 23.87±0.81 ab 22.67±0.68 b
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表 8 位点INRA005基因型与生产性状差异
Table 8. Differences on genotypes and productive traits at INRA005 loci
基因型
GenotypesAA AE AF AH BB DH FF GG HH 个体数
Individuals10 6 8 6 7 12 6 16 6 胴体长
Carcass length/cm65.80±1.72 ab 67.50±2.21 a 63.50±1.92 ab 60.67±2.21 b 63.86±2.05 ab 64.00±2.21 ab 63.38±1.36 ab 61.83±2.21 ab 62.60±1.40 ab 腿会斜长
Leg will be obliquely long/cm24.10±0.60 ab 22.67±0.77 b 23.00±0.67 ab 24.17±0.77 ab 22.43±0.71 b 25.00±0.77 a 23.38±0.47 ab 23.00±0.77 ab 23.93±0.49 ab 腿耻斜长
Legs are obliquely long/cm32.00±0.91 ABab 30.33±1.18 ABb 30.50±1.02 ABb 31.67±1.18 ABab 29.29±1.09 Bb 33.67±1.18 Aa 31.31±0.72 ABab 31.33±1.18 ABab 30.67±0.75 ABb 踝骨宽
Ankle width/cm6.60±0.19 ABa 7.00±0.25 Aa 6.50±0.21 ABab 6.17±0.25 ABab 6.71±0.23 ABa 6.50±0.25 ABab 6.06±0.15 Bb 6.33±0.25 ABab 6.20±0.16 Bb 胸宽
Chest width/cm24.40±1.00 a 25.83±1.29 a 25.13±1.12 a 21.00±1.29 b 23.57±1.20 ab 23.50±1.29 ab 24.38±0.79 a 22.17±1.29 ab 23.27±0.82 ab
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表 9 位点INRA006基因型与生产性状差异
Table 9. Differences on genotypes and productive traits at INRA006 loci
基因型
GenotypesAA AC AG BB BE CC EE FF 个体数
Individuals12 7 10 25 11 10 6 11 初生重
Primary weight/kg4.77±0.16 ABb 4.90±0.21 ABab 5.39±0.18 Aa 4.69±0.11 Bb 4.97±0.17 ABab 4.56±0.18 Bb 4.97±0.23 ABab 4.89±0.17 ABb 断奶重
Weaning weight/kg26.08±1.63 ab 31.38±2.13 a 29.40±1.78 ab 25.93±1.13 b 26.24±1.70 ab 27.66±1.78 ab 29.29±2.30 ab 28.78±1.70 ab 宰前重
Before slaughter/kg36.53±1.86 b 42.98±2.44 a 42.04±2.04 a 36.33±1.29 b 38.25±1.95 ab 36.20±2.04 b 37.68±2.64 ab 37.20±1.95 ab 胴体重
Carcass weight/kg18.98±1.07 b 22.99±1.40 a 22.25±1.17 a 18.81±0.74 b 19.97±1.11 ab 18.68±1.17 b 20.53±1.51 ab 19.42±1.11 ab 胸宽
Chest width/cm23.17±0.86 ABb 26.43±1.13 Aa 25.20±0.94 ABab 22.84±0.60 Bb 23.73±0.90 ABab 22.90±0.94 ABb 25.50±1.22 ABab 23.55±0.90 ABb
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表 10 位点INRA063基因型与生产性状差异
Table 10. Differences on genotypes and productive traits at INRA063 loci
基因型
GenotypesBB BE CC DD DG EE EF HH 个体数
Individuals10 8 11 9 7 11 7 7 初生重
Primary weight/kg4.65±0.19 b 4.73±0.21 ab 4.89±0.18 ab 5.07±0.20 ab 4.77±0.23 ab 4.78±0.18 ab 5.29±0.23 a 4.83±0.23 ab 腿会斜长
Leg will be obliquely long/cm24.50±0.57 a 23.25±0.63 ab 23.18±0.54 ab 24.44±0.60 a 24.71±0.68 a 22.64±0.54 b 23.57±0.68 ab 23.43±0.68 ab 腿耻斜长
Legs are obliquely long/cm30.00±0.89 Bb 32.13±1.00 ABab 30.73±0.85 ABb 32.67±0.94 ABa 33.86±1.06 Aa 30.91±0.85 ABb 31.71±1.06 ABab 32.29±1.06 ABab 臀宽
Hip width/cm20.90±0.74 ab 20.38±0.83 ab 19.73±0.71 b 20.22±0.78 ab 22.14±0.89 a 19.36±0.71 b 21.71±0.89 ab 19.29±0.89 b 胸宽
Chest width/cm24.20±0.97 ab 24.25±1.09 ab 22.91±0.93 ab 23.00±1.03 ab 24.14±1.16 ab 23.00±0.93 ab 25.00±1.16 a 21.71±1.16 b 胸深
Chest depth/cm24.60±0.83 a 23.75±0.93 ab 24.09±0.80 ab 24.33±0.88 ab 23.86±1.00 ab 22.18±0.80 b 24.00±1.00 ab 23.71±1.00 ab
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表 11 位点MAF214基因型与生产性状差异
Table 11. Differences on genotypes and productive traits at MAF214 loci
基因型 Genotypes AA BB BI CC CG DH 个体数 Individuals 9 13 9 9 10 8 断奶重 Weaning weight/kg 27.47±1.82 ab 24.85±1.51 b 29.52±1.82 ab 27.36±1.82 ab 27.93±1.72 ab 30.20±1.93 a 宰前重 Before slaughter/kg 38.96±2.23 ab 34.95±1.86 b 41.78±2.23 a 36.60±2.23 ab 37.89±2.12 ab 39.26±2.37 ab 胴体重 Carcass weight/kg 20.23±1.31 ab 18.13±1.09 b 22.19±1.31 a 18.52±1.31 ab 20.15±1.24 ab 20.51±1.39 ab 腿垂直长 Legs vertical length/cm 21.89±0.87 ab 21.15±0.72 b 21.11±0.87 b 21.56±0.87 b 24.00±0.82 a 23.00±0.92 ab 腿耻斜长 Legs are obliquely long/cm 31.22±0.92 ABa 31.92±0.76 Aa 32.33±0.92 Aa 32.00±0.92 Aa 28.50±0.87 Bb 30.25±0.97 ABab 踝骨宽 Ankle width/cm 6.33±0.20 AB 6.15±0.17 AB 6.78±0.20 A 6.33±0.20 AB 6.00±0.19 B 6.25±0.21 AB 臀宽 Hip width/cm 21.56±0.84 a 19.00±0.70 b 20.56±0.84 ab 20.67±0.84 ab 21.10±0.80 ab 21.13±0.89 ab 肩宽 Shoulder width/cm 20.67±0.94 ab 18.54±0.78 b 19.44±0.94 ab 19.22±0.94 ab 21.30±0.89 a 20.63±1.00 ab
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表 12 位点MCM527基因型与生产性状差异
Table 12. Differences on genotypes and productive trait at MCM527 loci
基因型
GenotypesAA AE BB BE CC CE DD EE 个体数
Individuals6 7 24 13 19 12 8 15 断奶重
Weaning weight/kg23.31±2.24 c 24.52±2.07 bc 29.41±1.12 a 29.03±1.52 ab 26.05±1.26 abc 27.67±1.58 abc 25.45±1.94 abc 26.38±1.41 abc 宰前重
Before slaughter/kg35.18±2.61 ABab 36.55±2.41 ABab 40.76±1.30 Aa 41.29±1.77 Aa 37.01±1.46 ABab 35.86±1.84 ABb 36.52±2.26 ABab 33.62±1.65 Bb 胴体重
Carcass weight/kg18.93±1.55 ab 17.70±1.43 b 21.37±0.77 a 21.54±1.05 a 19.31±0.87 ab 19.04±1.09 ab 18.69±1.34 ab 17.81±0.98 b 腿会斜长
Leg will be obliquely long/cm24.00±0.78 ab 23.43±0.72 ab 24.42±0.39 a 23.00±0.53 b 23.42±0.44 ab 23.17±0.55 ab 23.13±0.68 ab 24.07±0.49 ab 腿耻斜长
Legs are obliquely long/cm32.83±1.17 a 29.43±1.09 b 31.63±0.59 ab 30.92±0.80 ab 31.37±0.66 ab 31.25±0.83 ab 30.00±1.02 ab 31.80±0.74 ab 踝骨宽
Ankle width/cm6.17±0.24 Bb 6.29±0.22 ABb 6.50±0.12 ABab 6.92±0.16 Aa 6.05±0.13 Bb 6.33±0.17 ABb 6.38±0.21 ABb 6.13±0.15 Bb 胸宽
Chest width/cm23.00±1.27 ab 23.57±1.18 ab 24.50±0.64 ab 25.77±0.86 a 22.95±0.71 b 23.17±0.90 b 23.75±1.10 ab 23.07±0.80 b 肩宽
Shoulder width/cm19.17±1.14 ab 18.71±1.06 ab 20.54±0.57 a 20.08±0.78 ab 19.53±0.64 ab 19.42±0.81 ab 19.63±0.99 ab 18.67±0.72 b
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表 13 位点OARFCB20基因型与生产性状差异
Table 13. Differences on genotypes and productive traits at OARFCB20 loci
基因型
GenotypesBB BI CG CI DD DI EI GG II 个体数
Individuals7 10 8 6 9 8 9 6 15 断奶重
Weaning weight/kg25.95±2.17 ABb 32.62±1.82 Aa 24.98±2.03 Bb 29.82±2.35 ABab 25.73±1.92 ABb 27.46±2.03 ABab 26.19±1.92 ABb 23.75±2.35 Bb 27.62±1.48 ABb 宰前重
Before slaughter/kg36.39±2.64 ab 42.79±2.21 a 38.80±2.47 ab 39.34±2.86 ab 34.82±2.33 b 40.43±2.47 ab 36.83±2.33 ab 34.44±2.86 b 38.04±1.81 ab 胴体重
Carcass weight/kg18.76±1.49 ab 22.41±1.25 a 20.15±1.40 ab 21.70±1.61 ab 17.93±1.32 b 20.81±1.40 ab 18.77±1.32 b 17.96±1.61 b 20.20±1.02 ab 腿耻斜长
Legs are obliquely long/cm31.71±1.11 AB 29.10±0.93 B 31.13±1.04 AB 30.17±1.20 AB 30.56±0.98 AB 31.50±1.04 AB 30.78±0.98 AB 33.33±1.20 A 31.60±0.76 AB 臀宽
Hip width/cm20.86±0.91 ab 21.70±0.76 a 21.00±0.85 ab 20.83±0.99 ab 18.78±0.81 b 20.75±0.85 ab 19.11±0.81 b 19.67±0.99 ab 20.47±0.62 ab 胸宽
Chest width/cm23.00±1.15 ABb 26.40±0.96 Aa 24.50±1.07 ABab 24.00±1.24 ABab 22.22±1.01 Bb 25.13±1.07 ABab 22.89±1.01 ABb 22.67±1.24 ABb 23.93±0.78 ABab 肩宽
Shoulder width/cm19.71±1.01 ABab 21.50±0.85 Aa 20.88±0.95 ABab 21.00±1.09 ABab 17.56±0.89 Bb 20.13±0.95 ABab 18.44±0.89 ABb 18.50±1.09 ABb 19.40±0.69 ABab 胸深
Chest depth/cm22.57±0.98 b 25.70±0.82 a 24.75±0.92 ab 25.00±1.06 ab 23.11±0.87 b 23.88±0.92 ab 23.22±0.87 b 23.67±1.06 ab 24.00±0.67 ab
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[1] 国家畜禽遗传资源委员会组. 中国畜禽遗传资源志:［羊志］[M]. 北京: 中国农业出版社, 2011: 2 − 4. [2] ARRANZ J J, BAYON Y, PRIMITIVO F S. Genetic relationships among Spanish sheep using microsatellites [J]. Animal Genetics, 1998, 29(6): 435−440. doi: 10.1046/j.1365-2052.1998.296371.x [3] 王婷, 吴登俊. 微卫星分子标记分析四川绵羊群体遗传多样性 [J]. 中国畜牧杂志, 2018, 54(4):36−41.WANG T, WU D J. Study on genetic diversity of sheep populations in Sichuan by microsatellite markers [J]. Chinese Journal of Animal Science, 2018, 54(4): 36−41.(in Chinese) [4] 刘泽民, 王岩超, 张淑二, 等. 利用微卫星和线粒体D-loop区联合分析大尾寒羊的遗传多样性 [J]. 山东畜牧兽医, 2019, 40(7):1−5. doi: 10.3969/j.issn.1007-1733.2019.07.001LIU Z M, WANG Y C, ZHANG S E, et al. Analysis of genetic diversity of big tail Han Sheep using microsatellite and mitochondrial D-loop region [J]. Shandong Journal of Animal Science and Veterinary Medicine, 2019, 40(7): 1−5.(in Chinese) doi: 10.3969/j.issn.1007-1733.2019.07.001 [5] IBRAHIM M, AHMAD S, DURRANI I S, et al. Genetic polymorphism and bottleneck analysis of balkhi, hashtnagri, and michni sheep populations using microsatellite markers [J]. Animal Biotechnology, 2018, 29(3): 216−226. doi: 10.1080/10495398.2017.1366340 [6] 王可, 刘昭华, 崔绪奎, 等. 济宁青山羊微卫星标记多态性分析 [J]. 山东农业科学, 2017, 49(2):142−146.WANG K, LIU Z H, CUI X K, et al. Polymorphism analysis of microsatellite markers in Jining grey goat [J]. Shandong Agricultural Sciences, 2017, 49(2): 142−146.(in Chinese) [7] KUMAR S, DIXIT S P, VERMA N K, et al. Genetic diversity analysis of the gohilwari breed of Indian goat (Capra hircus) using microsatellite markers [J]. American Journal of Animal and Veterinary Sciences, 2009, 4(3): 49−57. doi: 10.3844/ajavsp.2009.49.57 [8] 乌仁套迪, 斯琴图雅, 白俊艳. 阿拉善沙漠双峰驼的微卫星多态性与体尺性状的关联分析 [J]. 畜牧与兽医, 2018, 50(9):4−9.WURENTAODI, SIQINTUYA, BAI J Y. Correlation between microsatellite polymorphism with body size traits of Alashan Desert Bactrian camels [J]. Animal Husbandry & Veterinary Medicine, 2018, 50(9): 4−9.(in Chinese) [9] 马丽娜, 马青, 李颖康. 滩羊12个微卫星基因座遗传多态性研究 [J]. 畜牧与饲料科学, 2018, 39(4):26−31. doi: 10.12160/j.issn.1672-5190.2018.04.008MA L N, MA Q, LI Y K. Genetic polymorphisms of twelve microsatellite loci in Tan sheep [J]. Animal Husbandry and Feed Science, 2018, 39(4): 26−31.(in Chinese) doi: 10.12160/j.issn.1672-5190.2018.04.008 [10] 梁庆玲, 牛志刚, 杨丹, 等. 陶赛特羊Carwell基因微卫星标记多态性与体重和体尺指标的相关性分析 [J]. 中国草食动物, 2012, 32(1):12−16.LIANG Q L, NIU Z G, YANG D, et al. Association between microsatellite markers polymorphism of carwell gene and growth traits of Dorset sheep [J]. China Herbivores, 2012, 32(1): 12−16.(in Chinese) [11] BARKER J F, MOORE S S, HETZEL D S, et al. Genetic diversity of Asian water buffalo (Bubalus bubalis): microsatellite variation and a comparison with protein-coding loci [J]. Animal Genetics, 1997, 28(2): 103−115. doi: 10.1111/j.1365-2052.1997.00085.x [12] HEARNE C M, GHOSH S, TODD J A. Microsatellites for linkage analysis of genetic traits [J]. Trends in Genetics, 1992, 8(8): 288−294. doi: 10.1016/0168-9525(92)90256-4 [13] BOTSTEIN D, WHITE R L, SKOLNICK M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms [J]. The American Journal of Human Genetics, 1980, 32(3): 314−331. [14] FARID A, O'REILLY E, DOLLARD C, et al. Genetic analysis of ten sheep breeds using microsatellite markers [J]. Canadian Journal of Animal Science, 2000, 80(1): 9−17. doi: 10.4141/A99-086 [15] VAJED EBRAHIMI M T, MOHAMMADABADI M, ESMAILIZADEH A. Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran [J]. Archives Animal Breeding, 2017, 60(3): 183−189. doi: 10.5194/aab-60-183-2017 [16] 周明亮, 陈明华, 庞倩, 等. 布拖黑绵羊微卫星标记遗传多样性研究 [J]. 草学, 2018(2):74−80. doi: 10.3969/j.issn.2096-3971.2018.02.014ZHOU M L, CHEN M H, PANG Q, et al. Polymorphic analysis of Butuo black sheep with microsatellite marker [J]. Journal of Grassland and Forage Science, 2018(2): 74−80.(in Chinese) doi: 10.3969/j.issn.2096-3971.2018.02.014 [17] 张瑞, 郎侠, 吴建平, 等. 岷县黑裘皮羊微卫星遗传多样性分析 [J]. 中国畜牧兽医, 2019, 46(8):2360−2370.ZHANG R, LANG X, WU J P, et al. Genetic diversity analysis of microsatellite in Minxian black fur sheep [J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(8): 2360−2370.(in Chinese) [18] 严林俊, 陈宏, 房兴堂, 等. 中国荷斯坦牛微卫星基因座遗传多态性研究 [J]. 西北农林科技大学学报(自然科学版), 2007, 35(7):23−27, 32.YAN L J, CHEN H, FANG X T, et al. Polymorphism distributions of microsatellite loci in Chinese Holstein cattle [J]. Journal of Northwest A & F University(Natural Science Edition), 2007, 35(7): 23−27, 32.(in Chinese) [19] 迟浩斌, 韩向敏, 郎侠, 等. 早胜牛微卫星多态性及其与生长性状的关联分析 [J]. 中国畜牧兽医, 2019, 46(7):1976−1985.CHI H B, HAN X M, LANG X, et al. Analysis of microsatellite polymorphism and its association with growth traits of Zaosheng cattle [J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(7): 1976−1985.(in Chinese) [20] 王斌, 昝林森, 杜宝民, 等. 宣汉牛微卫星DNA与生长发育性状的关联分析 [J]. 农业生物技术学报, 2018, 26(7):1174−1185.WANG B, ZAN L S, DU B M, et al. Association analysis of microsatellite DNA with growth and development traits of Xuanhan cattle(Bos taurus) [J]. Journal of Agricultural Biotechnology, 2018, 26(7): 1174−1185.(in Chinese) [21] 德吉, 巴贵, 吴玉江, 等. 西藏绒山羊的微卫星多态性与部分经济性状的关联分析 [J]. 西藏农业科技, 2019, 41(2):19−24. doi: 10.3969/j.issn.1005-2925.2019.02.007DE JI, BA GUI, WU Y J, et al. Correlation between microsatellite polymorphism and economic traits of Tibetan cashmere goat [J]. Tibet Journal of Agricultural Sciences, 2019, 41(2): 19−24.(in Chinese) doi: 10.3969/j.issn.1005-2925.2019.02.007 [22] 李标, 张瑞莹, 王小琪, 等. 滩羊微卫星标记多态性及与体尺性状关联分析 [J]. 生物技术通报, 2019(6):131−137.LI B, ZHANG R Y, WANG X Q, et al. Microsatellite polymorphism and its correlation analysis with body size traits of Tan sheep [J]. Biotechnology Bulletin, 2019(6): 131−137.(in Chinese) [23] 张瑞, 郎侠, 吴建平, 等. 岷县黑裘皮羊微卫星多态性及与生产性状的关联分析 [J]. 生物技术通报, 2019(11):55−63.ZHANG R, LANG X, WU J P, et al. Correlation analysis between microsatellite polymorphism and production traits in black fur sheep in Min County [J]. Biotechnology Bulletin, 2019(11): 55−63.(in Chinese) -
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