长粒、抗病杂交水稻恢复系福恢7076的选育与应用

陈丽萍; 谢鸿光; 吴方喜; 罗曦; 朱永生; 郑燕梅; 林强; 蔡秋华; 谢华安; 张建福

doi:10.19303/j.issn.1008-0384.2020.06.002

长粒、抗病杂交水稻恢复系福恢7076的选育与应用

doi: 10.19303/j.issn.1008-0384.2020.06.002

陈丽萍^{1, 2, 3,},
谢鸿光^{1, 2, 3},
吴方喜^{1, 2, 3},
罗曦^{1, 2, 3},
朱永生^{1, 2, 3},
郑燕梅^{1, 2, 3},
林强^{1, 2, 3},
蔡秋华^{1, 2, 3},
谢华安^{1, 2, 3, ,},
张建福^{1, 2, 3, ,}

1.
福建省农业科学院水稻研究所，福建　福州　350018
2.
农业农村部华南杂交水稻种质创新与分子育种重点实验室/福州（国家）水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/杂交水稻国家重点实验室华南研究基地，福建　福州　350003
3.
水稻国家工程实验室，福建　福州　350003

基金项目: 国家重点研发计划项目（2016YFD0101801）；福建省农业科学院博士基金资助项目（DC2017-6）；福建省农业科学院创新团队建设项目（STIT2017-1-1）

详细信息

作者简介:
陈丽萍（1986−），女，博士，助理研究员，主要从事水稻分子育种研究（E-mail：373200630@qq.com）

通讯作者:
谢华安（1941−），男，研究员，主要从事杂交水稻育种研究（E-mail：huaanxie@163.com）

张建福（1971−），男，博士，研究员，主要从事水稻分子育种研究（E-mail：jianfzhang@163.com）

中图分类号: S 511
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出版历程
- 收稿日期: 2019-11-24
- 修回日期: 2020-05-05
- 刊出日期: 2020-08-10

A New Blast-resistant Long-grain Hybrid Restorer Rice Line, Fuhui 7076

CHEN Liping^{1, 2, 3
,},
XIE Hongguang^{1, 2, 3},
WU Fangxi^{1, 2, 3},
LUO Xi^{1, 2, 3},
ZHU Yongsheng^{1, 2, 3},
ZHENG Yanmei^{1, 2, 3},
LIN Qiang^{1, 2, 3},
CAI Qiuhua^{1, 2, 3},
XIE Huaan^{1, 2, 3
, ,},
ZHANG Jianfu^{1, 2, 3
, ,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fujian Academy of Agricultural Sciences/Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, China/South China Bases of National Key Laboratory of Hybrid Rice for China, Fuzhou, Fujian　350003, China
3.
National Rice Engineering Laboratory of China, Fuzhou, Fujian　350003, China

摘要

摘要: 目的福建稻区稻瘟病多发，快速高效培育稻瘟病抗性较强的新品系，使其适应不断发生变异的稻瘟病菌生理小种，从而保持水稻主栽品种的抗瘟性，可为高效培育新品种奠定基础。方法于2008年，对农艺性状优良但抗稻瘟病性较弱的强恢复系（航2号）进行EMS（ems mutagenesis，甲基磺酸乙酯）化学诱变，经过多年多点不同生态区的定向培育，并结合抗瘟性鉴定，筛选具有目标性状的新恢复系。结果于2012年选育出籼型恢复系福恢7076。福恢7076既保持了航2号广亲和性、强恢复力、高配合力的优良特点，同时在品质和产量等方面均较航2号有所改善，稻瘟病抗性表现为中抗 (MR)。用福恢7076与赣香A配组的杂交水稻新组合赣优7076，参加2015–2016年云南省杂交籼稻品种试验，两年平均产量为10 711.5 kg·hm⁻²，比对照品种F优498增产3.24%，增产点率68.42%，2017年通过云南省农作物品种审定委员会审定（滇审稻2017009）。结论对农艺性状优良但抗稻瘟病性较弱的强恢复系（航2号）进行EMS化学诱变后，经过多年多点不同生态区的定向培育，再通过在稻瘟病多发稻区的筛选，加速了自然选择的过程，相比于长期的自然选择，可以更快地获得即具备亲本优良性状又具备稻瘟病抗性的新恢复系。
- 杂交水稻 /
- 恢复系 /
- 福恢7076 /
- 稻瘟病抗性 /
- 选育
Abstract: Objectives A new blast-resistant lines of rice suitable for cultivation in Fujian province was generated with an efficient breeding method. Methods Applying the EMS chemical mutagenesis, Hang 2 with excellent agronomic traits except blast-resistance was selected to breed a hybrid restorer line through directional cultivations in areas with varied ecological conditions. Disease-resistance of the lines was verified to finalize the process and hybrid selection. Results The hybrid Indica restorer line, Fuhui 7076, not only inherited the desirable agronomic traits of Hang 2, such as wide compatibility, strong resilience, and high combining ability, but also performed superior on the quality and yield as well as an MR (medium resistance) rating on blast. Utilizing Fuhui 7076 to cross with Ganxiang A, Ganyou 7076 gave a 2-year average yield of 10,711.5 kg/hectare at the 2015 and 2016 Yunnan Provincial Indica Hybrid Rice Trials. The yield was 3.24%, and the rate of increase 68.42%, higher than control, F You 498. In 2017 , Ganyou 7076 was certified by the Crop Certification Committee of Yunnan Province (Certificate No. 2017009). Conclusions With the EMS chemical mutagenesis, the agronomically desirable Hang 2 was bred to be blast-resistant. Through years of continued directional selection in blast-prone rice farming regions of varied ecosystems, the time required for natural mutation process was significantly shortened in the breeding of the disease-resistant variety.
- Hybrid rice /
- restorer line /
- Fuhui 7076 /
- rice blast resistance /
- breeding

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图 1 福恢7076和赣优7076的选育过程

Figure 1. Breeding of Fuhui 7076 and Ganyou 7076

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表 1 福恢7076和航2号的农艺性状及粒形比较

Table 1. Agronomic characteristics and grain shapes of Fuhui 7076 and Hang 2

供试材料 Variety	株高 Plant height/cm	每丛有效穗数 Panicle per cluster	穗长 Panicle length/cm	穗粒数Spikelet number per panicle	结实率 Spikelet fertility/%	千粒重 1 000-grain weight/g	长宽比 Ratio between length and width of grain	粒长 Grain lehgth/mm	粒宽 Grain width/mm	粒厚 Grain thickness/mm
航2号 Hang 2	132.65	11.5	29.86	305	90.15	29.45	3.32	9.93	3.01	1.99
福恢7076 Fuhui 7076	137.22	11.2	31.22^**	311	94.72^**	32.17^*	3.39	9.97	2.97	2.09^**
注：表示在0.05水平上差异显著，表示在0.01水平上差异极显著。表2同。 Note: significance at 0.05 level, ** at 0.01 level. The same as Table 2.

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表 2 福恢7076和航2号的米质比较

Table 2. Grain quality of Fuhui 7076 and Hang 2

供试材料 Variety	糙米率 Brown rice rate/%	精米率 Milled rice rate/%	整精米率 Full milled rice rate/%	长度 Grain length/mm	垩白粒率 Chalkness rate/%	垩白度 Chalkness degree/%	透明度 Transparency	糊化温度 Pasting temperature/℃	直链淀粉 Gel consistency/%
航2号 Hang 2	79.7	72.0	65.7	6.5	41.0	9.2	2	6.1	13.7
福恢7076 Fuhui 7076	77.1^*	71.5	62.7^*	6.8	16.0^**	4.2^**	2	6.4	14.1

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表 3 4个品种的粒型统计

Table 3. Grain shapes of 4 rice varieties

供试材料 Variety	千粒重 1 000-grain weight/g	长宽比 Ration between length and width of grain	粒长 Length of grain/mm	粒宽 Width of grain/mm	粒厚 Thickness of grain/mm
航2号 Hang 2	29.45	3.32	9.93	3.01	1.99
福恢7076 Fuhui 7076	32.17	3.39	9.97	2.97	2.09
赣优7076 Ganyou 7076	27.11	3.67	9.93	2.73	2.05
赣香A Ganxiang A	24.04	3.61	9.44	2.64	1.94

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表 4 4个品种粒长基因的变异位点检测

Table 4. Sequences of genotype related to grain length of 4 rice varieties

供试材料 Variety	GS3 （C/A）	GL3-1/qGL3 （1092C/A）	TGW6 （313G/-）
航2号 Hang 2	A	C	G
福恢7076 Fuhui 7076	A	C	G
赣优7076 Ganyou 7076	A	C	G
赣香A Ganxiang A	A	C	-

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表 5 福恢7076和航2号对稻瘟病菌的抗性鉴定

Table 5. Blast-resistance of Fuhui 7076 and Hang 2

供试材料 Variety	叶瘟 Leaf blast	穗瘟 Panicle blast	苗瘟 Seedling blast															抗性评价 Evaluation of resistance
			混合接菌 Mixed inoculation	菌株 Strain												致病菌数 Number of Pathogenic isolates	抗菌株率 Resistance frequency/%
			混合接菌 Mixed inoculation	S₁	S₂	S₃	S₄	S₅	S₆	S₇	S₈	S₉	S₁₀	S₁₁	S₁₂	致病菌数 Number of Pathogenic isolates	抗菌株率 Resistance frequency/%
航2号 Hang 2	MS	MS	S	S	S	S	S	S	S	S	S	S	S	S	R	11	8.33	MS
福恢7076 Fuhui 7076	MR	MR	S	R	R	R	R	R	R	R	S	S	S	S	MR	4	66.67	MR
注：苗瘟采用混合接种和分菌株接种鉴定；叶瘟和穗瘟为田间自然诱发鉴定（2019，上杭）；R表示“抗”，MR表示“中抗”，MS表示“中感”，S表示“感”；S_1～S₁₂为菌株代号，依次分别为：JY18026、NH17026、SH18073、NH19003、JL18053、NH19007、NH18044、NH18046、SW18055、XI18034、JL19019、JY18007；混合接菌为上述所有菌株混合后接菌。 Note: Blast-resistance of seedlings was determined by mixed and individual strain inoculations; those of leaves and panicles by natural induction in field at Shanghang in 2019; R: resistance; MR: moderate resistance; MS: moderate susceptible; S: susceptible; S1 to S12: codes for strain JY18026, NH17026, SH18073, NH19003, JL18053, NH19007, NH18044, NH18046, SW18055, XI18034, JL19019, and JY18007, respectively; mixed-strain inoculation used all strains of S₁ to S₁₂.

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