H<sub>2</sub>S对Cd胁迫下大麦幼苗逆境生理及光合作用的影响

李晓科; 武玉珍; 张谨华; 张义贤

doi:10.19303/j.issn.1008-0384.2020.10.011

H₂S对Cd胁迫下大麦幼苗逆境生理及光合作用的影响

doi: 10.19303/j.issn.1008-0384.2020.10.011

1.
晋中学院生物科学与技术学院，山西　晋中　030619
2.
山西大学生命科学学院，山西　太原　030006

基金项目: 山西省自然科学基金项目（201701D121083）；晋中学院2015年博士基金项目（2015006）

详细信息

作者简介:
李晓科（1979−），男，硕士，讲师，主要从事植物生理生态方面研究（E-mail：jzxylixiaoke@163.com）

中图分类号: X 171.5
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出版历程
- 收稿日期: 2020-02-18
- 修回日期: 2020-08-03
- 刊出日期: 2020-10-28

Effects of Hydrogen Sulfide on Physiology and Photosynthesis of Barley Seedlings under Cd-stress

1.
School of Biology Science and Technology, Jinzhong College, Jinzhong, Shanxi　030619, China
2.
School of Life Science, Shanxi University, Taiyuan, Shanxi　030006, China

摘要

摘要: 目的为揭示H₂S对Cd胁迫下大麦幼苗生长的胁迫效应规律，探讨H₂S提高大麦幼苗耐镉的作用机制，并为大麦种植区镉污染防治提供一定的科学依据。方法采用室内水培法，以晋科571大麦品种为研究对象，设置以CdCl₂（0.2 mmol·L⁻¹，处理中简称Cd）、外源H₂S供体NaHS（50.0 μmol·L⁻¹，处理中简称NaHS）和H₂S生成抑制剂羟胺HA（1.0 mmol·L⁻¹，处理中简称HA）形成的不同处理组：Cd、NaHS、HA、Cd+NaHS、Cd+HA。测定不同处理组对大麦幼苗生长、叶片损伤、渗透调节物质、抗氧化酶活性、叶绿素含量及光合特性的影响。结果与Cd组相比，外源H₂S缓解了Cd对幼苗生长的抑制和对叶片的损伤，表现为大麦幼苗根长、苗长、生物量增加，叶片相对电导率、丙二醛（MDA）及超氧阴离子（O₂⁻)含量降低；促进了光合作用，表现为叶绿素含量和净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）增加；提高了大麦幼苗抵抗外界不良环境的能力，表现为渗透调节物质（可溶性糖、脯氨酸、可溶性蛋白）含量和抗氧化酶（SOD、POD、CAT）活性增加。结论在外源H₂S供体NaHS溶液浓度为50.0 μmol·L⁻¹时，Cd胁迫对大麦幼苗造成的伤害得到了一定程度的缓解，外源H₂S能够增强大麦在Cd污染逆境中的适应能力。
- Cd胁迫 /
- 外源H₂S /
- 大麦 /
- 生理指标
Abstract: Objective Rule of H₂S played on the growth of barley seedlings under Cd-stress was investigated to understand the mechanism and means to improve Cd-tolerance of the plants. Method Treatment groups of Cd, NaHS, HA, Cd+NaHS, and Cd+HA with addition of CdCl₂ at 0.2 mmol·L⁻¹, exogenous H₂S donor NaHS at 50 μmol·L⁻¹ or H₂S synthesis inhibitor hydroxylamine HA at 1 mmol·L⁻¹ in medium were made for an indoor hydroponic barley seedling growth experiment. Effects of the treatments on the growth, leaf damage, osmotic regulator, antioxidant enzyme activity, chlorophyll content, and photosynthetic characteristics of the seedlings of Jinke 571 barley were monitored. Result The H₂S addition in the hydroponic medium lessened the growth inhibition and leaf damage on the seedlings induced by Cd-stress. It increased the root length, plant height, and seedling biomass; decreased the leaf relative conductivity, malondialdehyde (MDA) and superoxide anion (O₂⁻) contents; enhanced the photosynthesis with increased chlorophyll content, net photosynthetic rate, stomatal conductance, and transpiration rate; and, improved the resistance to the external adversity imposed on them with increased osmotic regulatory substances (e.g., soluble sugar, proline, soluble protein) and antioxidant enzymatic activities on SOD, POD, CAT. Conclusion Addition of H₂S donor NaHS at 50 μmol·L⁻¹ in hydroponic medium partially alleviated the ill-effects of Cd-stress on the barley seedlings suggesting its potential field application for barley farming to combat the pollution.
- Cd-stress /
- exogenous H₂S /
- Hordeum vulgare /
- physiological indices

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表 1 H₂S对Cd胁迫下大麦幼苗根长、苗长、生物量以及叶片损伤的影响

Table 1. Effects of H₂S on root length, plant length, biomass, and leaf damage of barley seedlings under Cd-stress

处理 Treatment group	根长 Root length/cm	苗长 Plant length/cm	生物量 Biomas/mg	MDA含量 MDA content/（µmol·g⁻¹）	相对电导率 Relative conductivity/%	O₂⁻含量 O₂⁻content/（nmol·g⁻¹）
CK	6.06±0.18 b	7.37±0.29 b	74.81±3.23 b	2.26±0.11 e	13.21±0.49 d	34.46±2.46 e
NaHS	6.26±0.13 a	8.15±0.31 a	76.11±2.99 a	2.01±0.26 f	10.83±0.54 e	33.45±2.71 d
Cd	4.71±0.20 c	6.23±0.25 d	60.19±2.51 d	3.81±0.23 c	32.32±1.35 b	54.19±3.36 b
Cd+NaHS	5.93±0.41 b	7.06±0.52 c	69.88±3.15 c	3.07±0.21 d	29.08±1.54 c	42.18±3.01 c
Cd+HA	3.18±0.27 d	4.91±0.65 e	48.66±3.61 e	4.02±0.13 a	37.06±1.53 a	60.42±2.26 a
HA	3.34±0.31 d	4.96±0.49 e	49.87±3.34 e	3.96±0.21 b	31.36±1.26 b	53.04±2.63 b
注：同列无相同小写字母表示差异显著（P＜0.05），下同。 Note: Date without the same lowercase letters in the same column represented significant difference（P＜0.05）, the same below.

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表 2 H₂S对Cd胁迫下大麦幼苗叶片渗透调节物质和抗氧化酶活性的影响

Table 2. Effects of H₂S on osmotic regulatory substances and antioxidant enzyme activities in leaves of barley seedlings under Cd-stress

处理 Treatment group	可溶性糖含量 Soluble sugar content/ （mg·g⁻¹）	脯氨酸含量 Proline content/ （µg·g⁻¹）	可溶性蛋白含量 Soluble protein content/（mg·g⁻¹）	SOD活性 SOD activity/ （U·g⁻¹）	POD活性 POD activity/ （U·g⁻¹·min⁻¹）	CAT活性 CAT activity/ （U·g⁻¹·min⁻¹）
CK	6.06±0.31 b	11.15±0.59 e	35.26±2.51 b	89.33±3.21 d	202.13±3.74 bc	400.98±6.61 a
NaHS	6.73±0.38 a	12.07±0.43 d	35.61±2.33 a	91.55±3.37 d	203.62±5.06 b	403.05±6.53 a
Cd	5.79±0.24 d	17.22±0.87 b	31.21±2.93 d	138.61±4.17 b	198.36±3.59 d	285.89±3.72 d
Cd+NaHS	6.12±0.26 b	19.76±0.69 a	34.55±2.19 c	157.39±4.75 a	216.29±4.64 a	326.21±4.39 c
Cd+HA	5.41±0.31 e	16.30±0.71 c	30.54±3.06 e	126.82±4.97 c	169.36±4.56 e	268.53±5.22 e
HA	5.98±0.27 c	10.33±0.59 f	35.08±3.27 b	86.41±3.72 e	200.17±4.47 c	352.62±4.82 b

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表 3 外源H₂S对Cd胁迫下大麦幼苗叶片叶绿素含量和光合特性的影响

Table 3. Effects of H₂S on chlorophyll content and photosynthetic characteristics of barley seedlings under Cd-stress

处理 Treatment group	叶绿素a含量 Chlorophyll a content/（mg·g⁻¹）	叶绿素b含量 Chlorophyll b content/（mg·g⁻¹）	净光合速率 Net photosynthetic rate/（μmol·m⁻²·s⁻¹）	气孔导度 Stomatal conductance/mmol·m⁻²·s⁻¹）	胞间CO₂浓度 Intercellular CO₂ concentration/ （μmol·mol⁻¹）	蒸腾速率 Transpiration rate/ （mmol·m⁻²·s⁻¹）
CK	0.93±0.03 b	0.55±0.01 b	13.63±1.33 a	0.23±0.01 b	189.02±12.03 b	8.35±0.03 b
NaHS	1.13±0.04 a	0.62±0.02 a	13.69±1.64 a	0.41±0.03 a	196.25±13.17 a	9.02±1.01 a
Cd	0.73±0.04 d	0.41±0.01 e	10.57±1.09 d	0.15±0.01 c	167.35±10.54 c	4.35±0.79 e
Cd+NaHS	0.84±0.03 c	0.51±0.02 c	11.26±1.23 c	0.21±0.03 b	166.81±12.46 c	5.68±1.06 c
Cd+HA	0.69±0.03 e	0.39±0.02 f	8.53±1.63 e	0.09±0.01 d	109.92±11.42 e	2.94±0.26 f
HA	0.83±0.02 c	0.47±0.01 d	11.87±1.92 b	0.14±0.02 c	128.39±15.32 d	5.16±1.24 d

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