Effects of Lactobacillus Addition on Fermentation and Quality of Animal Feed Made with Spent Substrate from Oyster Mushroom Cultivation
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摘要:
目的 探明乳酸菌对平菇菌糠发酵饲料品质和发酵进程的影响，以期提升发酵工艺水平，提高平菇菌糠作为畜禽饲料的利用价值。 方法 将2种乳酸菌（CCZZ1和HT1）单独及复合加入平菇菌糠中，并设置空白对照（CK），对4个试验处理进行室温下发酵效果的对比试验。4个处理的起始重复数均为18个，分别对每一处理发酵1、3、7、15、30、60 d时的发酵饲料进行化学成分和发酵品质的3个重复测定。 结果 发酵到7 d时，3个乳酸菌添加组CCZZ1、HT1和MIX组的pH值均显著低于CK组（P＜0.05），并比CK组提前23 d下降到4.2以下；CCZZ1、HT1和MIX组的乙酸和干物质含量均显著高于CK组（P＜0.05）；CCZZ1、HT1和MIX组在发酵过程中提高了乳酸含量，降低了粗蛋白含量；CCZZ1、HT1和MIX组的氨态氮、粗灰分、可溶性碳水化合物、酸性洗涤纤维、中性洗涤纤维、半纤维素、丙酸和丁酸的含量与CK组相比差异不显著。 结论 单独或复合添加乳酸菌不仅能改善平菇菌糠发酵饲料的品质，也能使平菇菌糠发酵饲料的发酵过程提前结束。 Abstract:Objective Effects of addition of lactic acid bacteria to the spent substrate of Pleurotus ostreatus cultivation on the fermentation and quality of the feed material for livestock and poultry industry were analyzed. Method Lactic acid bacteria CCZZ1 and HT1 were added alone or in combination (MIX) to the spent culture substrate prior to fermentation. A blank control (CK) was included in the 3-treatment experimentation. Initially, 18 replicates for each testing group were conducted at room temperature. Triplicated analytical data and fermentation process evaluation criteria on the 1st, 3rd, 7th, 15th, 30th, and 60th day were collected for the study. [Result] During the fermentation, the contents of lactic acid, acetic acid, propionic acid, and dry matters increased, while those of hemicellulose, butyric acid, and crude protein declined. And, ammonia nitrogen was significantly lower in MIX than in control (P＜0.05). After fermented for 7 d, the pH of the treatment groups were significantly lower than those of CK (P＜0.05), and reached below 4.2 in 23 d less than it took CK. The acetic acid and dry matter contents in the treatment groups were significantly higher than those in CK (P＜0.05), while lactic acid increased, and crude protein reduced as compared with CK. On the other hand, the contents of ammonia nitrogen, crude ash, soluble carbohydrate, acid washed fiber, neutral water washed fiber, hemicellulose, propionic acid, and butyric acid in the treatment groups did not significantly differ from those in CK. Conclusion The addition of lactic acid bacteria CCZZ1 and HT1 alone or in combination not only improved the quality of the fermented feed made from the spent mushroom culture substrate but also facilitated the fermentation process. -
Key words:
- Lactobacillus /
- spent mushroom culture substrate /
- anaerobic fermentation /
- feed quality
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图 1 不同处理、不同发酵时间的菌糠发酵饲料pH值
注：①图中每个时间点不同处理差异显著性标注从上到下依次是CK、CCZZI、HT1、MIX处理组；②（ ）内无相同字母表示同一时间点不同处理组间差异显著（P＜0.05），有相同字母者表示差异不显著（P＞0.05）；（ ）外无相同字母表示同一处理组不同时间点间差异显著（P＜0.05），有相同字母者表示差异不显著（P＞0.05）。图2～3、表3～12同。
Figure 1. Effect of treatments on pH of fermented feed material
Note:①The significant markers of different treatments at each time point in the figure from top to bottom are CK, CCZZI, HT1 and MIX treatment groups.（same as Figure1-3）②Different letters in "（ ）" indicate that there are significant differences among different treatment groups at the same time（P＜0.05）, the same letters or not marked letters show no significant difference（P＞0.05）.The different letters outside "（ ）" indicated that there were significant differences at different time points in the same treatment group（P＜0.05）, the same letters or not marked letters show no significant difference（P＞0.05）. Figure 2-3 and table 3-12 are the same.
图 2 不同处理、不同发酵时间的菌糠发酵饲料乳酸LA（FM）含量
Figure 2. Effect of treatments on lactic acid LA (FM) in fermented feed material
图 3 不同处理、不同发酵时间的菌糠发酵饲料乙酸AA（FM）含量
Figure 3. Effect of treatments on acetic acid AA (FM) in fermented feed material
表 1 菌糠的化学成分
Table 1. Chemical composition of spent mushroom culture substrate
pH DM/ % WSC/% CP /% NDF/% ADF/% HC/% 5.42 39.89 3.63 9.62 76.03 64.01 12.02 注：（1）表中营养成分均为菌糠干物质（DM）测定值；（2）WSC：可溶性碳水化合物，CP：粗蛋白，NDF：中性洗涤纤维，ADF：酸性洗涤纤维，HC：半纤维素。
Note:（1）all nutrients in the table are measured by bacterial bran dry matter（DM）.（2）WSC: Soluble carbohydrate, CP: Crude protein, NDF: Neutral detergent fiber, ADF: Acid detergent fiber, HC:Hemicellulose.
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表 2 菌糠的微生物组成及数量
Table 2. Microorganisms and microbial counts in spent mushroom culture substrate
[单位：lg(cfu·g−1)] 乳酸菌
Lactic acid bacteria好气性细菌
Aerobic bacteria酵母菌
Yeast霉菌
Mold2.23 4.98 2.35 1.23
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表 3 不同处理、不同发酵时间的菌糠发酵饲料氨态氮AN（AN/TN）含量
Table 3. Effect of treatments on ammonia nitrogen AN (AN/TN) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 0.08±0.01（a）b 0.07±0.01（a）b 0.08±0.00（a）b 0.04±0.00（b）c 3 0.03±0.00（b）f 0.04±0.00（a）e 0.03±0.00（b）f 0.04±0.00（a）c 7 0.04±0.00（a）e 0.04±0.00（a）e 0.04±0.00（a）e 0.04±0.00（a）c 15 0.06±0.01（a）d 0.05±0.00（ab）d 0.05±0.01（ab）d 0.04±0.00（b）c 30 0.07±0.00（a）c 0.06±0.00（ab）c 0.06±0.00（ab）c 0.05±0.01（b）b 60 0.14±0.03（a）a 0.08±0.00（b）a 0.09±0.01（b）a 0.10±0.01（b）a
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表 4 不同处理、不同发酵时间的菌糠发酵饲料丙酸PA（FM）含量
Table 4. Effect of treatments on propionic acid PA (FM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 0.02±0.00（a）a 0.02±0.00（a）b 0.02±0.00（a）b 0.02±0.01（a）b 3 0.01±0.01（a）a 0.02±0.01（a）b 0.02±0.00（a）b 0.02±0.01（a）b 7 0.02±0.00（a）a 0.02±0.00（a）b 0.01±0.01（a）b 0.02±0.01（a）b 15 0.02±0.00（a）a 0.02±0.01（a）b 0.01±0.01（a）b 0.02±0.00（a）b 30 0.03±0.00（b）a 0.04±0.00（a）a 0.04±0.01（a）a 0.04±0.01（a）a 60 0.04±0.04（a）a 0.04±0.01（a）a 0.04±0.01（a）a 0.04±0.02（a）a
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表 5 不同处理、不同发酵时间的菌糠发酵饲料丁酸BA（FM）含量
Table 5. Effect of treatments on butyric acid BA (FM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 0.04±0.01（a）a 0.04±0.01（a）a 0.03±0.02（a）a 0.03±0.00（a）a 3 0.03±0.01（a）a 0.01±0.01（b）b 0.02±0.00（ab）a 0.03±0.01（a）a 7 0.01±0.00（a）b 0.01±0.01（a）b 0.02±0.02（a）a 0.03±0.02（a）a 15 0.01±0.01（a）b 0.03±0.02（a）ab 0.02±0.01（a）a 0.01±0.00（a）b 30 0.01±0.00（a）b 0.01±0.01（a）b 0.01±0.01（a）a 0.01±0.01（a）b 60 0.01±0.01（b）b 0.03±0.01（a）ab 0.02±0.01（ab）a 0.01±0.01（b）b
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表 6 不同处理、不同发酵时间的菌糠发酵饲料干物质DM含量
Table 6. Effect of treatments on dry matters (DM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 36.09±0.72（a）c 36.18±0.61（a）b 36.10±0.32（a）bc 36.47±0.18（a）bc 3 35.91±0.35（a）c 35.34±0.25（a）c 36.04±0.76（a）c 36.32±0.21（a）cd 7 34.96±0.24（b）d 35.67±0.28（a）bc 35.96±0.44（a）c 35.69±0.44（a）d 15 37.05±0.21（a）b 37.39±0.71（a）a 37.38±1.39（a）ab 37.15±0.56（a）a 30 37.09±0.15（a）b 36.12±0.20（bc）b 36.39±0.23（b）bc 35.87±0.03（c）cd 60 40.25±0.66（a）a 38.06±0.15（b）a 38.20±0.02（b）a 37.04±0.33（c）ab
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表 7 不同处理、不同发酵时间的菌糠发酵饲料粗灰分Ash含量
Table 7. Effect of treatments on ash (DM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 11.87±0.07（b）b 12.08±0.16（ab）b 12.35±0.23（a）a 11.98±0.16（b）a 3 11.72±0.11（b）b 12.22±0.24（a）ab 12.69±0.08（a）a 11.85±0.05（b）a 7 11.78±0.29（c）b 12.31±0.06（b）ab 12.66±0.19（a）a 11.78±0.07（c）a 15 11.89±0.56（b）b 12.36±0.24（a）ab 12.56±0.14（a）a 11.76±0.30（b）a 30 12.30±0.38（ab）b 12.46±0.12（ab）a 12.70±0.30（a）a 11.98±0.28（b）a 60 14.02±0.70（a）a 10.94±0.08（b）c 10.52±0.19（b）b 11.23±0.19（b）b
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表 8 不同处理、不同发酵时间的菌糠发酵饲料粗蛋白CP含量
Table 8. Effect of treatments on crude protein CP (DM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 7.01±0.09（a）ab 7.08±0.08（a）b 6.90±0.13（a）b 6.98±0.10b（a）b 3 6.81±0.11（a）bc 7.05±0.14（a）b 6.90±0.10（a）b 6.97±0.60（a）b 7 7.12±0.11（b）a 7.34±0.10（a）a 7.13±0.14（b）a 7.02±0.07（b）b 15 6.98±0.05（b）abc 7.17±0.07（a）b 7.15±0.08（a）a 7.27±0.03（a）a 30 6.79±0.11（a）c 6.86±0.07（a）c 6.80±0.14（a）b 6.81±0.11（a）c 60 6.79±0.19（a）c 6.37±0.11（b）d 6.38±0.08（b）c 6.20±0.01（b）d
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表 9 不同处理、不同发酵时间的菌糠发酵饲料可溶性碳水化合物WSC含量
Table 9. Effect of treatments on water soluble carbohydrate WSC (DM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 0.60±0.14（a）ab 0.48±0.04（a）b 0.49±0.05（a）bc 0.56±0.14（a）ab 3 0.68±0.12（a）a 0.67±0.18（a）a 0.69±0.32（a）a 0.66±0.07（a）a 7 0.53±0.06（a）ab 0.48±0.04（a）b 0.61±0.17（a）ab 0.48±0.02（a）bc 15 0.46±0.04（b）b 0.47±0.01（b）b 0.42±0.04（c）c 0.51±0.03（a）bc 30 0.58±0.04（a）ab 0.63±0.08（a）ab 0.60±0.02（a）ab 0.48±0.05（b）bc 60 0.57±0.11（a）ab 0.66±0.04（a）a 0.55±0.08（a）abc 0.38±0.01（b）c
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表 10 不同处理、不同发酵时间的菌糠发酵饲料酸性洗涤纤维ADF含量
Table 10. Effect of treatments on acid washing fiber ADF (DM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 49.30±1.67（b）b 49.97±3.33（b）e 55.53±2.23（a）cd 56.65±1.76（a）d 3 51.35±7.27（a）b 56.48±0.82（a）d 51.89±3.44（a）d 54.50±2.53（a）d 7 59.32±3.31（a）a 62.66±2.48（a）bc 60.36±4.26（a）bc 61.25±1.48（a）bc 15 61.12±2.06（a）a 58.94±1.64（a）cd 58.53±3.16（a）bc 59.71±0.82（a）c 30 63.34±0.77（b）a 64.70±2.02（ab）ab 66.59±1.16（a）a 62.92±1.67（b）ab 60 64.19±0.91（ab）a 66.84±1.69（a）a 63.77±1.92（b）ab 65.55±1.20（ab）a
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表 11 不同处理、不同发酵时间菌糠发酵饲料的中性洗涤纤维NDF含量
Table 11. Effect of treatments on neutral washing fiber NDF (DM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 60.56±4.86（a）c 61.05±4.11（a）c 66.96±4.26（a）c 62.37±0.92（a）d 3 67.00±3.47（a）b 71.17±1.18（a）b 67.18±5.35（a）c 67.50±2.44（a）c 7 76.61±0.85（a）a 78.25±2.12（a）a 74.95±2.93（a）ab 74.88±2.35（a）ab 15 75.10±2.13（a）a 70.88±3.98（a）b 72.22±3.51（a）bc 75.25±5.35（a）ab 30 75.47±1.58（b）a 75.64±2.05（b）ab 79.43±0.60（a）a 76.85±1.50（ab）a 60 69.93±1.59（b）b 72.95±1.43（a）b 69.44±1.28（b）bc 71.27±1.55（ab）bc
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表 12 不同处理、不同发酵时间的菌糠发酵饲料半纤维素HC含量
Table 12. Effect of treatments on hemicellulose HC (DM) in fermented feed material
（单位：%） 发酵时间 Fermentation Time/d CK CCZZ1 HT1 MIX 1 11.26±3.22（a）b 11.08±0.93（a）c 11.43±2.50（a）a 5.72±0.85（b）b 3 15.65±3.99（a）ab 14.68±0.59（a）ab 15.29±4.56（a）a 13.00±0.22（a）a 7 17.29±3.29（a）a 15.59±0.66（a）a 14.60±1.34（a）a 13.63±2.37（a）a 15 13.98±1.95（a）ab 11.93±3.00（a）bc 13.68±0.43（a）a 15.55±4.79（a）a 30 12.12±0.84（a）b 10.95±2.33（a）c 12.84±0.58（a）a 13.93±3.16（a）a 60 5.74±1.39（a）c 6.12±0.40（a）d 5.67±0.84（a）b 5.72±0.46（a）b
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[1] 于磊, 姜明, 徐丽萍, 等. 食用菌菌糠的研究及其再利用 [J]. 黑龙江科技信息, 2014(29):73.YU L, JIANG M, XU L P, et al. Study and reuse of edible fungus chaff [J]. Heilongjiang Science and Technology Information, 2014(29): 73.(in Chinese) [2] 陈锡, 姜明, 李双双, 等. 菌糠的综合利用研究进展 [J]. 中国园艺文摘, 2017, 33(8):43−44. doi: 10.3969/j.issn.1672-0873.2017.08.019CHEN X, JIANG M, LI S S, et al. Research progress on comprehensive utilization of fungus chaff [J]. Zhongguo Yuanyi Wenzhai, 2017, 33(8): 43−44.(in Chinese) doi: 10.3969/j.issn.1672-0873.2017.08.019 [3] 卫智涛, 周国英, 胡清秀. 食用菌菌渣利用研究现状 [J]. 中国食用菌, 2010, 29(5):3−6, 11. doi: 10.3969/j.issn.1003-8310.2010.05.001WEI Z T, ZHOU G Y, HU Q X. Research and utilization of edible fungi residue [J]. Edible Fungi of China, 2010, 29(5): 3−6, 11.(in Chinese) doi: 10.3969/j.issn.1003-8310.2010.05.001 [4] MA B. Conversion of Masonia tree sawdust and cotton plant by product into feed by white rot fungus (Pleurotus sajor caju) [J]. African Journal of Biotechnology, 2006, 5(19): 503−504. [5] 张丽影, 潘婷, 苏宇, 等. 平菇和杏鲍菇两种菌糠的营养价值分析 [J]. 广东微量元素科学, 2015, 22(5):24−28.ZHANG L Y, PAN T, SU Y, et al. Nutrition analysis of Pleurotus ostreatus and Pleurotus eryngii spent mushroom substrate [J]. Guangdong Trace Elements Science, 2015, 22(5): 24−28.(in Chinese) [6] 李志香, 蔡元丽. 菌糠发酵饲料的研究 [J]. 中国畜牧兽医, 2003, 30(5):8−9. doi: 10.3969/j.issn.1671-7236.2003.05.003LI Z X, CAI Y L. Study on fermentation feed of fungus chaff [J]. China Animal Husbandry & Veterinary Medicine, 2003, 30(5): 8−9.(in Chinese) doi: 10.3969/j.issn.1671-7236.2003.05.003 [7] 曹启民, 张永北, 宋绍红, 等. 灵芝菌糠发酵饲料对育肥猪生产性能的影响 [J]. 中国饲料, 2013(9):39−41.CAO Q M, ZHANG Y B, SONG S H, et al. Effects of Ganoderma WMLE feed on production performance of finishing pigs [J]. China Feed, 2013(9): 39−41.(in Chinese) [8] 罗茂春, 柯志君, 邱丰艳, 等. 白玉菇菌糠分析及其发酵饲料制备工艺优化 [J]. 东北农业大学学报, 2014, 45(9):84−88, 94. doi: 10.3969/j.issn.1005-9369.2014.09.013LUO M C, KE Z J, QIU F Y, et al. Nutritional composition analysis of spent white Hypsizygus marmoreus substrate and technological conditions optimization for fermentation feed preparation [J]. Journal of Northeast Agricultural University, 2014, 45(9): 84−88, 94.(in Chinese) doi: 10.3969/j.issn.1005-9369.2014.09.013 [9] 刘伯实. 纤维素酶产生菌的筛选及其在菌糠开发中的利用[D]. 天津: 天津大学, 2008.LIU B S. Screening of cellulase-producing bacteria and its utilization in the development of fungus residue[D]. Tianjin: Tianjin University, 2008. [10] 郑有坤, 易敏, 陈建州, 等. 微生物发酵对香菇菌糠饲料品质的影响 [J]. 西南农业学报, 2013, 26(3):1143−1147.ZHENG Y K, YI M, CHEN J Z, et al. Influence of microorganism fermentation on feed quality of spent mushroom compost of Lentinula edodes [J]. Southwest China Journal of Agricultural Sciences, 2013, 26(3): 1143−1147.(in Chinese) [11] 李志涛, 林冬梅. 菌糠发酵饲料开发应用研究 [J]. 畜牧与兽医, 2015, 47(9):119−121.LI Z T, LIN D M. Study on the Development and Application of fungus chaff fermented Feed [J]. Animal Husbandry & Veterinary Medicine, 2015, 47(9): 119−121.(in Chinese) [12] 李向林. 饲草饲料的利用与发展对策 [J]. 饲料与畜牧(新饲料), 2000(3):33−35.LI X L. Utilization and Development Countermeasures of forage Feed [J]. Feed and Animal Husbandry (new feed), 2000(3): 33−35.(in Chinese) [13] BRODERICK G A, KANG J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media [J]. Journal of Dairy Science, 1980, 63(1): 64−75. doi: 10.3168/jds.S0022-0302(80)82888-8 [14] HAN K, COLLINS M, VANZANT E S, et al. Bale density and moisture effects on alfalfa round bale silage [J]. Crop Science, 2004, 44(3): 914−919. doi: 10.2135/cropsci2004.9140 [15] 杨胜. 饲料分析及饲料质量检测技术[M]. 北京: 北京农业大学出版社, 1993: 19–33. [16] 森本宏. 家畜栄養実験法[M]. 東京: 養賢堂発行, 1971: 1–149. [17] 张丽英. 饲料分析及饲料质量检测技术[M]. 北京: 中国农业大学出版社, 2007. [18] 傅彤. 微生物接种剂对玉米青贮饲料发酵进程及其品质的影响[D]. 北京: 中国农业科学院, 2005.FU T. Effects of microbial inoculants on fermentation process and quality of corn silage[D]. Beijing: Chinese Academy of Agricultural Sciences, 2005. [19] 陆亚珍, 王恒昌, 申远航, 等. 杏鲍菇菌糠的营养价值评价及其在羊日粮中的应用效果 [J]. 安徽农业科学, 2017, 45(3):117−118, 166. doi: 10.3969/j.issn.0517-6611.2017.03.040LU Y Z, WANG H C, SHEN Y H, et al. Nutritional value evaluation of Pleurotus eryngii bran and its application effects in the diet of goat and sheep [J]. Journal of Anhui Agricultural Sciences, 2017, 45(3): 117−118, 166.(in Chinese) doi: 10.3969/j.issn.0517-6611.2017.03.040 [20] 白全亮. 菌糠的化学营养价值及其在饲料中的应用 [J]. 科技资讯, 2016, 14(29):62−63.BAI Q L. The chemical and nutritional value of fungus chaff and its application in feed [J]. Science & Technology Information, 2016, 14(29): 62−63.(in Chinese) [21] 秦翠静, 程书梅, 陈宝江, 等. 菌糠在饲料中的应用研究 [J]. 食用菌, 2016, 38(2):5−6. doi: 10.3969/j.issn.1000-8357.2016.02.002QIN C J, CHENG S M, CHEN B J, et al. Study on the application of fungus chaff in feed [J]. Edible Fungi, 2016, 38(2): 5−6.(in Chinese) doi: 10.3969/j.issn.1000-8357.2016.02.002 [22] 王英超, 潘亮, 高艳萍. 菌糠发酵过程中的生化指标动态变化 [J]. 贵州农业科学, 2013, 41(1):143−145. doi: 10.3969/j.issn.1001-3601.2013.01.043WANG Y C, PAN L, GAO Y P. Dynamic changes of biochemical indicators during mushroom dregs fermentation process [J]. Guizhou Agricultural Sciences, 2013, 41(1): 143−145.(in Chinese) doi: 10.3969/j.issn.1001-3601.2013.01.043 [23] MCDONALD P, HENDERSON A R, HERLOW S J E. The Biochemistry of Silage(Second Edition)[M]. Southampton: Chalcombe Publications, 1991. [24] 陈鑫珠, 李文杨, 刘远, 等. 发酵时间对菌糠青贮饲料发酵品质的影响 [J]. 草学, 2018(1):28−32. doi: 10.3969/j.issn.2096-3971.2018.01.006CHEN X Z, LI W Y, LIU Y, et al. The effect of fermentation time on fermentation quality of fungus chaff silage [J]. Pruataculture & Animal Husbandry, 2018(1): 28−32.(in Chinese) doi: 10.3969/j.issn.2096-3971.2018.01.006 [25] 陈鑫珠, 李文杨, 刘远, 等. 菌糠发酵饲料品质的动态变化 [J]. 中国草食动物科学, 2018, 38(3):76−78. doi: 10.3969/j.issn.2095-3887.2018.03.020CHEN X Z, LI W Y, LIU Y, et al. Dynamic changes of fermentation feed quality of fungus chaff [J]. China Herbivore Science, 2018, 38(3): 76−78.(in Chinese) doi: 10.3969/j.issn.2095-3887.2018.03.020 [26] 侯建建, 白春生, 张庆, 等. 单一和复合乳酸菌添加水平对苜蓿青贮营养品质及蛋白组分的影响 [J]. 草业科学, 2016, 33(10):2119−2125. doi: 10.11829/j.issn.1001-0629.2015-0752HOU J J, BAI C S, ZHANG Q, et al. Effects of different additive amount of single and multiple lactic acid bacteria on the silage quality and protein fractions of alfalfa [J]. Pratacultural Science, 2016, 33(10): 2119−2125.(in Chinese) doi: 10.11829/j.issn.1001-0629.2015-0752 -
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