三系杂交中稻新品种M76优212的选育

陈春霞; 王洪飞; 游晴如; 周鹏; 涂诗航; 郑菲艳; 董瑞霞; 郑家团; 黄庭旭

doi:10.19303/j.issn.1008-0384.2020.12.001

三系杂交中稻新品种M76优212的选育

doi: 10.19303/j.issn.1008-0384.2020.12.001

福建省农业科学院水稻研究所，福建　福州　350018

基金项目: 福建省科技计划公益类专项（2019R1023-4、2019R1023-6）；国家重点研发计划项目（2017YFD0100101）；福建省农业科学院科技创新团队项目（STIT2017-1-1）

详细信息

作者简介:
陈春霞（1979−），女，博士，助理研究员，研究方向：主要从事水稻分子辅助育种研究（E-mail：158625731@qq.com）

通讯作者:
黄庭旭（1964−），男，硕士，研究员，研究方向：主要从事水稻遗传育种（E-mail：610143535@qq.com）

中图分类号: S 511
计量
- 文章访问数: 554
- HTML全文浏览量: 84
- PDF下载量: 59
- 被引次数: 0
出版历程
- 收稿日期: 2019-12-24
- 修回日期: 2020-10-22
- 刊出日期: 2020-12-31

Breeding of a New Three-line Hybrid Rice Variety M76 You 212

Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou　Fujian　350018, China

摘要

摘要: 目的利用抗病不育系与优质恢复系测交配组选育出抗病杂交稻新品种。方法以粤晶丝苗2号作母本，与抗稻瘟病中间材料U03杂交，F₁干种子用350Gy60Co-γ射线辐照，从M₂代分离群体中发现1株小粒、矮秆、直立的穗突变株。突变株后代经系谱法选择及稻瘟病抗性鉴定之后于2012年选择田间代号为12HR6-12的株系与抗病不育系M76A少量制种，2013–2014年分别在福建省建阳市、海南省三亚市参加多点品比试验，恢复系定名为福恢212。2015年M76优212参加福建省中稻组区域试验，产量、抗性表现突出，2016年续试，2017年进入生产试验。结果 M76优212在连续2年区试中的平均产量达8 763.60 kg·hm⁻²，比对照品种（Ⅱ优明86）增产8.61%，丰产性、稳产性表现好，中抗（MR）稻瘟病，米质较优。于2018年通过福建省农作物品种审定（闽审稻20180007）。结论 M76优212品种具有产量高、米质较优和抗病性好等优点，适宜福建省作中稻种植。
- 杂交水稻 /
- 辐射诱变 /
- 福恢212 /
- M76优212
Abstract: Objective Breeding a new resistant hybrid rice variety by testing cross of resistant sterile line and high quality restorer line. Method F₁ hybrid seeds of Yuejingsimiao 2 and blast-resistant intermediate material U03 were treated by 350Gy60Co-γirradiation, a mutant character as small grains, dwarf and erect panicle was found in M₂. After the Pedigree selection and identification of rice blast resistance, the plant with field number 12HR6-12 was selected to hybrid with sterile line M76A. In 2013-2014, multipoint variety comparative tests were carried out in Jianyang, Fujian Province and Sanya, Hainan Province, and the new restorer line was named Fuhui 212. In 2015, M76 you 212 performed very well with a high yield and rice blast-resistant at the mid-season rice regional trials in Fujian.It is entering continuing tries and produce trial at 2016 and 2017 respectively. Result The mean yield of M76 You 212 is 8763.60 kg•hm^-2 at the regional trials in Fujian in two consecutive years, which was 8.61% higher than that of the check variety, Ⅱ Youming 86. Over all, M76 You 212 showed high and stable yield, moderate resistance to rice blast and good grain quality at regional trials, it was certified by the Crop Variety Approval Committee of Fujian in 2018. Conclusion M76 You 212, the new three-line hybrid rice variety, is characterized by high yield, good grain quality and blast resistance and suitable for middle-season rice cultivation in Fujian Province.
- hybrid rice /
- radiation induced /
- Fuhui 212 /
- M76 you 212

HTML全文

图 1 恢复系福恢212及新品种M76优212的选育过程

Figure 1. Breeding processes of Fuhui 212 and M76 you 212

下载: 全尺寸图片幻灯片

表 1 M76优212在福建省中稻区域试验中的产量表现

Table 1. Yield performance of M76 you 212 in Regional Trials on Middle-season Rice Varieties in Fujian

年份 Year	产量幅度 Range of yield/ （kg·hm⁻²）	平均产量 Average yield/ （kg·hm⁻²）	比CK增产 Compared with CK/（kg·hm⁻²）	比CK增产率 Ratio compared with CK/%	试验点数 Number of test points	参试品种数 Number of varieties tested
2015	6349.95～9947.55	8486.10	1053.88	14.18	7	12
2016	7375.05～9787.50	9040.95	267.59	3.05	8	12
平均 Average		8763.53	660.74	8.62

下载: 导出CSV

表 2 M76优212在福建省中稻生产试验中的产量表现

Table 2. Yield performance of M76 you 212 in Preoduction Trialson Middle-season Rice Varieties in Fujian

试验点 Location	产量 Yield/（kg·hm⁻²）	比 CK增产 Compared with CK/（kg·hm⁻²）	比 CK增产率 Ratio compared with CK/%
武夷山市 Wuyishan	7650.00	1117.50	17.11
大田县 Datian	9286.50	567.00	6.51
龙岩市新罗区 Xinluo	9061.50	1905.00	26.60
建阳市 Jianyang	9811.50	531.00	5.71
尤溪县 Youxi	9610.50	3417.00	53.00
周宁县 Zhouning	7317.00	852.00	13.20
平均 Average	7390.95	1398.45	18.92

下载: 导出CSV

表 3 M76优212在福建省晚稻区试中的农艺性状表现

Table 3. Agronomic characteristics of M76 you 212 in regional and production trails held in Fujian on Late varieties

品种 Name of varieties	年份 Year	试验类型 Type of test	生育期 Growth period/d	株高 Plant height/cm	穗长 Ear length/cm	有效穗 Effective panicle no./×10⁴ 穗·hm⁻²	成穗率 Percentage of earbearing tiller/%	总粒数 Spikelets per panicle	着粒密度 Spikelet density/ No·cm⁻¹	结实率 Seed setting rate/%	千粒重 1000 grain weight/g
M76优212 M76 you 212	2015	区试 Regional trial	136.60	124.30	25.40	196.50	67.90	217.50	8.56	81.15	28.30
M76优212 M76 you 212	2016	区试 Regional trial	142.30	125.90	25.70	216.00	76.60	223.70	8.70	80.90	27.10
	平均 Average		139.45	125.10	25.55	206.25	72.25	220.60	8.63	81.03	27.70
II优明86 II you ming 86（CK）	2015	区试 Regional trial	137.70	123.70	26.10	168.00	55.50	179.80	6.89	88.69	28.90
II优明86 II you ming 86（CK）	2016	区试 Regional trial	141.40	125.60	26.00	210.00	68.20	203.50	7.83	90.10	28.60
	平均 Average		139.55	124.65	26.05	189.00	61.85	191.65	7.36	89.40	28.75
M76优212 M76 you 212	2017	生产试验 Production test	145.17	120.70	25.72	206.40	—	266.18	10.35	73.80	27.27
II优明86 II you ming 86（CK）	2017	生产试验 Production test	143.83	119.17	26.51	397.65	—	208.40	7.86	87.08	28.60

下载: 导出CSV

表 4 M76优212在福建省中稻区试中的抗性表现

Table 4. Rice blast resisitance of M76 you 212 displayed in Regional Trials on Middle-season Rice Varieties in Fujian

品种 Varieties	年份 Year	点数 Points	田间叶瘟鉴定 Identification of leaf pest in field			田间穗颈瘟鉴定 Identification of ear neck distemper in field			室内鉴定 Identification in greenhouse		综合评价 Comprehensive evaluation
品种 Varieties	年份 Year	点数 Points	发病率 Prevalence/%	病情指数 Disease index	抗性类型 Type of resistance	发病率 Prevalence/%	病情指数 Disease index	抗性类型 Type of resistance	抗菌株率 Antibacterial strain rate/%	抗性类型 Type of resistance	综合评价 Comprehensive evaluation
M76优212 M76 you 212	2015	4	1.13	0.26	R	17.63	4.40	R	83.33	R	R
	2016	4	22.50	7.00	MR	26.63	7.49	MR	100.00	R	MR
	平均 average		11.82	3.63	MR	22.13	5.95	MS	91.67	R	MS
II优明86 II you ming 86（CK）	2015	4	82.00	56.91	HS	98.13	82.79	HS	4.17	HS	HS
	2016	4	51.75	28.36	S	100.00	83.42	HS	30.43	S	HS
	平均 Average		66.88	42.64	HS	99.07	83.11	HS	17.30	HS	HS

下载: 导出CSV

表 5 M76优212的种子商品性

Table 5. The seed commodity of M76 you 212

年份 Year	发芽数 Number of germination	发芽率 Germination rate/%	正常株数 Number of normal plants	纯度 Purity/%	种子饱满度 Seed fullness	色泽 Color
2017	1 773	88.65	1 766	99.61	饱满 Full	杆黄色 Light yellow
2018	1 688	84.40	1 674	99.17	饱满 Full	杆黄色 Light yellow
平均 Average	1 730.5	86.53	1 720	99.39

下载: 导出CSV

参考文献(18)

[1]	张昌泉, 赵冬生, 李钱峰, 等. 稻米品质性状基因的克隆与功能研究进展 [J]. 中国农业科学, 2016, 49(22):4267−4283. doi: 10.3864/j.issn.0578-1752.2016.22.002 ZHANG C Q, ZHAO D S, LI Q F, et al. Progresses in research on cloning and functional analysis of key genes involving in rice grain quality [J]. Scientia Agricultural Sinica, 2016, 49(22): 4267−4283.(in Chinese) doi: 10.3864/j.issn.0578-1752.2016.22.002
[2]	刘贵富, 陈明江, 李明, 等. 水稻育种行业创新进展 [J]. 植物遗传资源学报, 2018, 19(3):416−429. LIU G F, CHEN M J, LI M, et al. Advances and innovation of rice breeding [J]. Journal of Plant Genetic Resources, 2018, 19(3): 416−429.(in Chinese)
[3]	黄庭旭, 郑家团, 游晴如, 等. 福建省杂交水稻选育研究现状与展望 [J]. 福建农业学报, 2012, 27(3):312−318. doi: 10.3969/j.issn.1008-0384.2012.03.020 HUANG T X, ZHENG J T, YOU Q R, et al. Status and prospect of the research on the breeding of hybrid rice in Fujian Province [J]. Fujian Journal of Agricultural Sciences, 2012, 27(3): 312−318.(in Chinese) doi: 10.3969/j.issn.1008-0384.2012.03.020
[4]	斯琴图雅, 高德玉, 张玉宝, 等. 我国水稻辐射诱变育种现状 [J]. 黑龙江科学, 2013, 4(5):42−43, 83. doi: 10.3969/j.issn.1674-8646.2013.05.020 SIQIN T Y, GAO D Y, ZHANG Y B, et al. Status of induced mutation breeding by radiation for rice in China [J]. Heilongjiang Science, 2013, 4(5): 42−43, 83.(in Chinese) doi: 10.3969/j.issn.1674-8646.2013.05.020
[5]	李伟, 吴超, 李正和, 等. ⁶⁰Co-γ辐射对香软型水稻品种‘滇屯502’的诱变效应 [J]. 云南农业大学学报(自然科学版), 2016, 31(1):7−15. LI W, WU C, LI Z H, et al. Mutagenic effect of ⁶⁰Co-γ on aromatic and soft rice Variety 'Diantun 502' [J]. Journal of Yunnan Agricultural University(Natural Science Edition), 2016, 31(1): 7−15.(in Chinese)
[6]	鲍根良, 严文潮, 张小明, 等. 粳稻优质米突变体E203的诱变选育研究 [J]. 核农学报, 2002, 16(5):268−271. doi: 10.3969/j.issn.1000-8551.2002.05.004 BAO G L, YAN W C, ZHANG X M, et al. Study on selection and evaluation of Japonica rice mutant E203 with superior grain quality [J]. Acta Agriculturae Nucleatae Sinica, 2002, 16(5): 268−271.(in Chinese) doi: 10.3969/j.issn.1000-8551.2002.05.004
[7]	陈慧茹. 镉在水稻亚种间的吸收转运和积累差异及其低积累突变种质的创建[D]. 合肥: 中国科学技术大学, 2019. CHEN H R. Absorption, transport, and accumulation of cadmium in rice cultivars and identification of low-cadmium rice mutant[D]. Hefei: University of Science and Technology of China, 2019. (in Chinese).
[8]	黄荣华. 优质高产杂交糯稻新组合糯两优7号的选育与应用 [J]. 江西农业大学学报, 2020, 42(4):641−646. HUANG R H. Breeding and application of new waxy hybrid rice combination Nuoliangyou No. 7 with good grain quality and high yield [J]. Acta Agriculturae Universityatis Jiangxiensis, 2020, 42(4): 641−646.(in Chinese)
[9]	王俊梅, 叶胜海, 翟荣荣等. 耐草甘膦水稻种质资源的创制和鉴定 [J]. 核农学报, 2017, 31(3):432−439. doi: 10.11869/j.issn.100-8551.2017.03.0432 WANG J M, YE S H, ZHAI R R, et al. Creation and identification of glyphosate-tolerant rice germplasm resources [J]. Journal of Nuclear Agricultural Sciences, 2017, 31(3): 432−439.(in Chinese) doi: 10.11869/j.issn.100-8551.2017.03.0432
[10]	章清杞, 李美德, 黄荣华. 巨胚糯稻恢复系福巨糯2号的选育 [J]. 杂交水稻, 2020, 35(204):27−30. ZHANG Q Q, LI M D, HUANG R H. Breeding of waxy rice restorer line Fujunuo 2 with giant embryo [J]. Hybrid Rice, 2020, 35(204): 27−30.(in Chinese)
[11]	陆艳婷, 陈金跃, 张小明, 等. 浙江省水稻辐射育种研究进展 [J]. 核农学报, 2017, 31(8):1500−1508. doi: 10.11869/j.issn.100-8551.2017.08.1500 LU Y T, CHEN J Y, ZHANG X M, et al. Advance in study and application of the rice breeding by radiation in Zhejiang province [J]. Journal of Nuclear Agricultural Sciences, 2017, 31(8): 1500−1508.(in Chinese) doi: 10.11869/j.issn.100-8551.2017.08.1500
[12]	王奉斌, 魏玉波, 布哈丽且木, 等. 辐射诱变选育水稻品种新稻12号的实践与探讨 [J]. 安徽农学通报, 2010, 16(3):65, 93. WANG F B, WEI Y B, BU H L Q M, et al. Practice and discussion on new variety Xindao 12 under irradiation breeding [J]. Anhui Agricultural Science Bulletin, 2010, 16(3): 65, 93.(in Chinese)
[13]	吴钟, 陈文阳, 高云. 广东省水稻诱变育种进展及展望 [J]. 广东农业科学, 2013(8):4−7. doi: 10.3969/j.issn.1004-874X.2013.08.002 WU Z, CHEN W Y, GAO Y. Achievements and perspective outlook of rice induced mutation breeding in Guangdong [J]. Guangdong Agricultural Sciences, 2013(8): 4−7.(in Chinese) doi: 10.3969/j.issn.1004-874X.2013.08.002
[14]	何秀英, 陈粤汉, 廖耀平, 等. 优质稻主导品种粤晶丝苗2号的选育过程与应用情况 [J]. 作物杂志, 2011(5):131−132. doi: 10.3969/j.issn.1001-7283.2011.05.038 HE X Y, CHEN Y H, LIAO Y P, et al. Breeding and application of leading variety with good quality Yuejingsimiao 2 [J]. Crops, 2011(5): 131−132.(in Chinese) doi: 10.3969/j.issn.1001-7283.2011.05.038
[15]	毛大梅, 林芳, 吴明基, 等. 高产杂交中稻新组合M76优3301 [J]. 杂交水稻, 2018, 33(1):84−85. MAO D M, LIN F, WU M J, et al. M76 You 3301, a new high-yielding medium hybrid rice combination [J]. Hybrid Rice, 2018, 33(1): 84−85.(in Chinese)
[16]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 稻瘟病测报调查规范: GB/T 15790—2009[S]. 北京: 中国标准出版社, 2009.
[17]	李黎红, 张宇. 我国杂交水稻优势、面临挑战与国际化策略 [J]. 中国稻米, 2019, 25(4):1−4. doi: 10.3969/j.issn.1006-8082.2019.04.001 LI L H, ZHANG Y. The advantages, challenges and internationalization strategies of China's hybrid rice [J]. China Rice, 2019, 25(4): 1−4.(in Chinese) doi: 10.3969/j.issn.1006-8082.2019.04.001
[18]	林兆松. 福建省核农学研究三十年(1959–1989) [J]. 福建稻麦科技, 1991, 9(1):1−5. LIN Z S. Thirty Years of Research in Nuclear Agriculture in Fujian Province(1959–1989) [J]. Fujian Science and Technology of Rice and Wheat, 1991, 9(1): 1−5.(in Chinese)