小麦百农矮抗58幼苗对Na<sub>2</sub>SO<sub>4</sub>胁迫响应及阈值鉴定

刘铎; 白爽; 齐学斌; 宁东峰; 梁志杰; 郭魏; 李平

doi:10.19303/j.issn.1008-0384.2021.01.002

小麦百农矮抗58幼苗对Na₂SO₄胁迫响应及阈值鉴定

doi: 10.19303/j.issn.1008-0384.2021.01.002

刘铎^{1, 2,},
白爽³,
齐学斌^{1, 4},
宁东峰¹,
梁志杰^{1, 2, 4},
郭魏^{1, 4},
李平^{1, 4, ,}

1.
中国农业科学院农田灌溉研究所，河南　新乡　453002
2.
中国农业科学院农业水资源高效安全利用重点开放实验室，河南　新乡　453002
3.
北京市八大处公园管理处，北京 100041
4.
农业农村部农产品质量安全水环境因子风险评估实验室，河南　新乡　453002

基金项目: 中央级公益性行业科研院所基本科研业务费项目（FIRI2019-04-01）；国家重点研发计划支持项目（2017YFD0800403）；中国农业科学院科技创新工程项目（CAAS-ASTIP-FIRI-03）

详细信息

作者简介:
刘铎（1991−），男，博士，助理研究员，主要从事植物逆境生理生态研究（E-mail：ld258825@126.com）

通讯作者:
李平（1979−），男，博士，副研究员，主要从事农业资源环境研究（E-mail：firilp@163.com）

中图分类号: S 512
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- 文章访问数: 706
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- 被引次数: 0
出版历程
- 收稿日期: 2020-10-12
- 修回日期: 2020-12-10
- 网络出版日期: 2021-02-08
- 刊出日期: 2021-01-31

Response and Tolerance Threshold of Bainong Aikang-58 Wheat Seedlings toward Na₂SO₄ Stress

LIU Duo^{1, 2
,},
BAI Shuang³,
QI Xuebin^{1, 4},
NING Dongfeng¹,
LIANG Zhijie^{1, 2, 4},
GUO Wei^{1, 4},
LI Ping^{1, 4
, ,}

1.
Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang, Henan 　453002, China
2.
Key Laboratory of High-efficiency and Safe Utilization of Agriculture Water Resources of CAAS, Xinxiang, Henan 　453002, China
3.
Administering Department of Beijing Badachu Park, Beijing 　100041, China
4.
Water Environment Factor Risk Assessment Laboratory of Agricultural Products Quality and Safety, Ministry of Agriculture, Xinxiang, Henan 　453002, China

摘要

摘要: 目的百农矮抗58是我国小麦的主栽品种之一，探讨百农矮抗58幼苗对Na₂SO₄胁迫响应机制，并界定其对Na₂SO₄胁迫耐受阈值，进而为进一步深入研究提供基础数据，也为农业生产实践提供科学依据与理论指导。方法通过水培试验，设置不同浓度（0、20、40、60、80、100 mmol·L⁻¹）Na₂SO₄胁迫处理，通过测定株高、鲜重、生物量、各种抗氧化酶活性、质膜透性、丙二醛（MDA）含量及渗透调节物质含量，研究Na₂SO₄胁迫对小麦幼苗生长与生理的影响。结果随着Na₂SO₄浓度的升高，小麦幼苗的株高、鲜重及生物量均下降，小麦幼苗的各种抗氧化酶活性会随着Na₂SO₄浓度的升高而不断升高，当超过一定阈值后会下降，MDA含量和质膜透性会随着Na₂SO₄浓度的升高而升高，最大值分别为对照的3.20倍和4.94倍；小麦幼苗叶片的叶绿素含量随着Na₂SO₄浓度的升高而下降；小麦体内的可溶性糖含量随着Na₂SO₄浓度的升高呈先上升后下降的变化趋势，在80 mmol·L⁻¹时可溶性糖含量最高；脯氨酸含量则随着Na₂SO₄浓度的升高而升高，最大值为对照的8.51倍。结论根据试验结果界定小麦百农矮抗58耐Na₂SO₄胁迫阈值为80～100 mmol·L⁻¹。
- 盐碱地 /
- 矮抗58 /
- Na₂SO₄胁迫 /
- 阈值
Abstract: Objective Bainong Aikang-58 is one of the major wheat cultivars in China. To investigate the response mechanism of Bainon Aikang-58 seedling to Na₂SO₄stress was discussed and to define its tolerance threshold to Na₂SO₄ stress was determined so as to provide basic data support for further research and scientific basis and and theoretical guidance for agricultural production practice. Method Effects of Na₂SO₄ stress at 0, 20, 40, 60, 80, and 100 mmol·L⁻¹ on the growth and physiology of the wheat seedlings were studied in a hydroponic experimentation. Result As the Na₂SO₄ concentration in the medium increased, the plant height, fresh weight, and biomass of the seedlings decreased. The activity of each antioxidant enzyme in wheat seedlings would continuously increase with the increase of Na₂SO₄ concentration, and would decrease after exceeding a certain threshold. The membrane permeability and content of MDA in the synplasmic membrane increase with increasing of Na₂SO₄ concentration to maximize 3.2 and 4.94 times of the control. The chlorophyll content in the leaves were decreased with the increase of Na₂SO₄ concentration. The soluble sugar content in wheat increased first and then decreased with the increase of Na₂SO₄ concentration. The soluble sugar concentration was the highest at 80 mmol·L⁻¹Whereas, the proline content kept increasing to reach a maximum that was 8.51 times of control. Conclusion The tolerance threshold of Bainong Aikang-58 wheat seedling toward Na₂SO₄ stress was determined to be 80−100 mmol·L⁻¹.
- saline-alkali soil /
- Bainong Aikang-58 /
- Na₂SO₄ stress /
- tolerance threshold

HTML全文

图 1 Na₂SO₄胁迫对小麦幼苗生长影响

注：*代表与对照（CK）相比差异达到显著水平（P<0.05）,**代表与对照相比差异达到极显著水平（P<0.01），图2～4相同

Figure 1. Effect of Na₂SO₄ stress on growth of wheat seedslings

Note: * represents significant difference compared with control group (CK) (P < 0.05); ** represents extremely significant difference compared with control group (P < 0.01). Figures 2-4 were the same

下载: 全尺寸图片幻灯片

图 2 Na₂SO₄胁迫对小麦幼苗抗氧化系统影响

Figure 2. Effect of Na₂SO₄ stress on antioxidant system of wheat seedlings

下载: 全尺寸图片幻灯片

图 3 Na₂SO₄胁迫对小麦幼苗叶绿素含量的影响

Figure 3. Effect of Na₂SO₄ stress on chlorophyll content in leaves of wheat seedlings

下载: 全尺寸图片幻灯片

图 4 Na₂SO₄胁迫对小麦幼苗渗透调节物质含量的影响

Figure 4. Effect of Na₂SO₄ stress on content of osmosis regulators in wheat seedlings

下载: 全尺寸图片幻灯片

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