生长素IAA诱导红萍结孢的研究

徐国忠; 郑向丽; 王俊宏; 邓素芳; 杨有泉; 应朝阳

doi:10.19303/j.issn.1008-0384.2021.06.014

生长素IAA诱导红萍结孢的研究

doi: 10.19303/j.issn.1008-0384.2021.06.014

徐国忠^{1, 2,},
郑向丽^{1, 2},
王俊宏^{1, 2},
邓素芳^{1, 2},
杨有泉^{1, 2},
应朝阳^{1, 2}

1.
福建省农业科学院农业生态研究所，福建　福州　350013
2.
国家红萍种质资源中心，福建　福州　350013

基金项目: 福建省科技计划公益类专项（2018R1010-2）；现代农业产业技术体系建设专项（CARS-22）

详细信息

作者简介:
徐国忠（1965−），男，研究员，主要从事生态农业研究（E-mail：xuguozhong178@126.com）

中图分类号: S 555.1
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- 被引次数: 0
出版历程
- 收稿日期: 2021-01-18
- 修回日期: 2021-05-24
- 网络出版日期: 2021-06-06
- 刊出日期: 2021-06-28

Auxin IAA-Induced Azolla Sporulation

XU Guozhong^{1, 2
,},
ZHENG Xiangli^{1, 2},
WANG Junhong^{1, 2},
DENG Sufang^{1, 2},
YANG Youquan^{1, 2},
YING Zhaoyang^{1, 2}

1.
Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
National Azolla Germplasm Resource Center, Fuzhou, Fujian　350013, China

摘要

摘要: 目的探索生长素IAA诱导红萍结孢的可行性。方法在网室自然条件下进行，采用质量浓度为0（对照）、1、5、10、15、20 μg·mL⁻¹的生长素（IAA）对生长期的红萍1001、3001和4018进行不同浓度喷施处理，每周喷施一次，至结孢出现停止喷施。止喷施。结果 IAA处理5个月后，1、5和10 μg·mL⁻¹的细绿萍1001开始结孢，质量浓度为 5 μg·mL⁻¹时，结孢率、孢子果数量、孢子果䧳雄比均优于其他处理及对照。在5 μg·mL⁻¹时，结孢率为15.0%、孢子果数量为6.31个、孢子果䧳雄比为0.62∶1；在10 μg·mL⁻¹时，结孢率为6.3%、孢子果数量为4.12个、孢子果䧳雄比为0.53∶1；在1 μg·mL⁻¹时，结孢率为4.7%、孢子果数量为3.90个、孢子果䧳雄比为0.51∶1。其他品种、处理都未能诱导出孢子果。细绿萍1001经IAA处理后，在 1、5和10 μg·mL⁻¹时萍体的大小和C/N比有明显提高，其余处理和对照无显著差异；卡州萍3001和小叶萍4018处理萍体大小和C/N比均无显著差异。结论生长素IAA能够诱导细绿萍1001结孢，其中IAA质量浓度为5 μg·mL⁻¹时诱导效果最佳，可在生产上进行应用。
- 激素 /
- 生长素 /
- 红萍 /
- 结孢 /
- 诱导
Abstract: Objective Auxin IAA-induced sporulation of Azolla was investigated. Method Under natural conditions in a netted chamber, auxin IAA solutions in concentration of 0 (control), 1, 5, 10, 15, or 20 μg·mL⁻¹ was sprayed once-a-week on Azolla filiculoides Lamarck 1001, A. caroliniana wild 3001, and A. microphylla Kaulfus 4018 during vegetative growth period for the duration until sporocarps appeared . Result After five months of spraying with 1, 5, or 10 μg·mL⁻¹ IAA solution, sporocarps began to form only on Azolla 1001. The sporulation rate, spore count, and female/male ratio were 15.0%, 6.31, and 0.62 ∶ 1, respectively, under the 5 μg·mL⁻¹ application, which significantly differed from 6.3%, 4.12, and 0.53 ∶ 1, respectively, under the 10 μg·mL⁻¹ application, 4.7%, 3.90, and 0.51 ∶ 1, respectively, under the 1 μg·mL⁻¹ application, and control. In addition, the size and C/N ratio of Azolla 1001 significantly increased by the spraying, as compared to control, whereas none observed on the other two ferns. Conclusion Auxin IAA could induce the sporulation of A. filiculoides Lamarck 1001. Spraying IAA at the concentration of 5 μg·mL⁻¹ on the ferns appeared feasible for field application.
- hormone /
- auxin /
- Azolla /
- sporulation /
- induction

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表 1 生长素IAA对3种不同红萍品种（系）结孢的诱导结果

Table 1. IAA-induced sporulation on 3 Azolla cultivars (lines)

激素 Hormone	质量浓度 Treatment concentration/ (μg·mL⁻¹)	是否结孢 The sporulation of Azolla
激素 Hormone	质量浓度 Treatment concentration/ (μg·mL⁻¹)	1001	3001	4018
IAA	0（CK）	否	否	否
IAA	1	是	否	否
IAA	5	是	否	否
IAA	10	是	否	否
IAA	15	否	否	否
IAA	20	否	否	否

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表 2 生长素IAA对3种红萍萍体大小（长度和宽度）的影响

Table 2. Effects of IAA applications on size (length and width) of 3 Azolla cultivars (lines)

激素 Hormone	质量浓度 Treatment concentration/ (μg·mL⁻¹)	长度 Length/mm			宽度 Breadth/mm
激素 Hormone	质量浓度 Treatment concentration/ (μg·mL⁻¹)	1001	3001	4018	1001	3001	4018
IAA	0（CK）	24.32±0.24 Cc	14.07±0.09 Aa	24.26±0.10 Aa	11.95±0.03 Cc	7.99±0.04 Aa	12.01±0.03 Aa
IAA	1	33.90±0.09 Bb	14.08±0.04 Aa	24.24±0.10 Aa	15.98±0.02 Bb	8.00±0.03 Aa	12.00±0.04 Aa
IAA	5	34.97±0.05 Aa	14.13±0.09 Aa	24.25±0.12 Aa	16.30±0.09 Aa	8.01±0.05 Aa	12.01±0.02 Aa
IAA	10	34.05±0.21 Bb	14.12±0.04 Aa	24.32±0.12 Aa	16.02±0.03 Bb	7.97±0.02 Aa	12.04±0.03 Aa
IAA	15	24.46±0.12 Cc	14.16±0.04 Aa	24.28±0.07 Aa	12.02±0.03 Cc	7.99±0.05 Aa	12.00±0.05 Aa
IAA	20	24.55±0.09 Cc	14.14±0.04 Aa	24.40±0.06 Aa	12.02±0.03 Cc	8.00±0.03 Aa	12.03±0.03 Aa
注：同列数值后不同小写字母表示处理间差异显著（P＜0.05），大写字母表示处理间差异极显著（P＜0.01）。 Note: Values followed by different lowercase letters indicate significant difference among treatments (P＜0.05), capital letters indicate very significant differences between treatments (P＜0.01).

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表 3 生长素IAA对3种红萍C/N比例的影响

Table 3. Effects of IAA applications on C/N ratio of 3 Azolla cultivars

序号 No.	IAA质量浓度 IAA concentration/ (μg·mL ⁻¹)	C/N比例 The ratio of C and N
序号 No.	IAA质量浓度 IAA concentration/ (μg·mL ⁻¹)	1001	3001	4018
1	0（CK）	10.3±0.2 Cc	9.6±0.7 Aa	10.7±0.7 Aa
2	1	15.5±0.5 Bb	9.7±0.7 Aa	10.6±0.5 Aa
3	5	18.0±1.0 Aa	9.5±0.7 Aa	10.8±0.4 Aa
4	10	16.2±1.1 ABb	9.8±0.7 Aa	10.9±0.5 Aa
5	15	10.5±0.6 Cc	9.6±0.6 Aa	10.8±0.5 Aa
6	20	10.6±0.5 Cc	9.6±0.3 Aa	10.9±0.7 Aa
注：同列数值后不同小写字母表示处理间差异显著（P＜0.05），大写字母表示处理间差异极显著（P＜0.01）。 Note: Values followed by different lowercase letters indicate significant difference among treatments (P ＜0.05), those followed by capital letters, extremely significant differences between treatments (P＜0.01).

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表 4 生长素IAA对细绿萍1001的诱导效果

Table 4. Effect of IAA on sporulation induction of Azolla 1001

激素 Hormone	处理质量浓度 Treatment concentration/ (μg·mL⁻¹)	结孢率 Sporocarp formation rate/%	结孢数量 The sporocarps number/个	䧳雄比 The sporocarp female male ratio
IAA	0（CK）	0.00±0.00Cd	0.00±0.00 Dd	0.00±0.00 Cc
IAA	1	4.70±0.60Bc	3.90±0.08 Cc	0.51±0.02 Bb
IAA	5	15.00±2.00 Aa	6.31±0.17 Aa	0.62±0.02 Aa
IAA	10	6.30±0.60 Bb	4.12±0.02 Bb	0.53±0.03 Bb
IAA	15	0.00±0.00 Cd	0.00±0.00 Dd	0.00±0.00 Cc
IAA	20	0.00±0.00 Cd	0.00±0.00 Dd	0.00±0.00 Cc
注：同列数值后不同小写字母表示处理间差异显著（P＜0.05），大写字母表示处理间差异极显著（P＜0.01）。 Note: Values followed by different lowercase letters indicate significant difference among treatments (P ＜0.05), those followed by capital letters, extremely significant differences between treatments (P＜0.01).

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