基于高通量测序的中国水仙根际土壤微生物群落组成

李媛媛; 张琼; 陈小煌; 张敬虎; 陆銮眉

doi:10.19303/j.issn.1008-0384.2021.08.012

基于高通量测序的中国水仙根际土壤微生物群落组成

doi: 10.19303/j.issn.1008-0384.2021.08.012

1.
闽南师范大学生物科学与技术学院/闽台特色园林植物福建省高等学校重点实验室，福建　漳州　363000
2.
闽南师范大学化学化工与环境学院，福建　漳州　363000

基金项目: 福建省科技计划对外合作重点项目（2018I0014）；漳州市自然科学基金项目（ZZ2020J10）；漳州市科技重大专项（ZZ2019ZD01）；闽南师范大学校长基金项目（4206/L21918）

详细信息

作者简介:
李媛媛（1989−），女，博士，讲师，主要从事环境微生物学研究（E-mail：liyuanyuan9215@163.com）

通讯作者:
陆銮眉（1968−），女，教授，主要从事观赏园艺学研究（E-mail：358070295@qq.com）

中图分类号: S 682
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- 文章访问数: 420
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- 被引次数: 0
出版历程
- 收稿日期: 2021-03-28
- 修回日期: 2021-05-21
- 刊出日期: 2021-08-28

High-throughput Sequencing on Microbial Community in Rhizosphere Soil of Chinese Narcissus

1.
Provincial Key Laboratory of Landscape Plants with Fujian and Taiwan Characteristics/College of the Bioscience and Technology, Minnan Normal University, Zhangzhou, Fujian　363000, China
2.
College of the Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou, Fujian　363000, China

摘要

摘要: 目的明确中国水仙植株根际土壤微生物群落的组成特征。方法采用Illumina Miseq高通量测序技术对水仙根际土壤微生物样品的保守基因区进行测序及生物信息学分析，阐明中国水仙根际土壤的细菌、真菌和古菌群落结构组成，并对水仙根际土壤微生物的优势菌属进行深入分析。结果共获得优化序列175 840条，基于97%序列相似度，聚类为2 680个OTUs。优势细菌类群是绿弯菌门 Chloroflexi（30.86%）和变形菌门 Proteobacteria（20.67%），真菌以子囊菌门 Ascomycota（84.94%）为主，属水平上球毛壳菌 Chaetomium globosum（28.15%）和散子囊菌 Eurotiales（25.01%）占较高比例。古菌类群主要为奇古菌门 Thaumarchaeota（51.40%）、深古菌门 Bathyarchaeota（25.98%）和广古菌门 Euryarchaeota（20.65%）。其中，来自奇古菌门的SCG类群（25.67%）和嗜酸性氨氧化古菌 Candidatus_Nitrosotalea（12.93%）占较高比例。结论中国水仙根际土壤微域具有丰富多样的微生物类群，这对于开发和利用水仙根际土壤微生物资源具有重要意义。
- 中国水仙 /
- 根际土壤微生物 /
- 高通量测序 /
- 细菌 /
- 真菌 /
- 古菌
Abstract: Objective The microbial community in rhizosphere soils of Chinese narcissuses was analyzed. Method The Illumina Miseq high-throughput sequencing technology was used to obtain the relevant conserved gene regions of the microbes in soil specimens from Chinese narcissus planting lots. The community structures and distributions of dominant species of bacteria, fungi, and archaea in the rhizosphere soils were analyzed. Result In total, 175 840 optimized sequences were obtained and clustered from the specimens into 2 680 representative OTUs with a 97% similarity. The dominant bacteria were Chloroflexi (30.86%) and Proteobacteria (20.67%). Among the fungi, Ascomycota (84.94%) significantly overshadowed the others, with Chaetomium globosum (28.15%) and Ascomycetes (25.01%) accounted for the greater proportions. On archaea, Thaumarchaeota (51.40%), Bathyarchaeota (25.98%), and Euryarchaeota (20.65%) were the major phyla that had 25.67% SCG and 12.93% acidophilic ammonia oxidizing members. Conclusion The rhizosphere soils of Chinese narcissuses harbored diverse and rich microbial species. The information obtained would aid the development and utilization of the natural resources.
- Chinese narcissuses /
- rhizosphere soil microorganisms /
- high-throughput sequencing /
- bacteria /
- fungi /
- archaea

HTML全文

图 1 中国水仙根际微生物群落丰富度分布

Figure 1. Relative abundance of microorganisms in Chinese narcissus rhizosphere soil at phylum level

下载: 全尺寸图片幻灯片

图 2 中国水仙根际Top15微生物属水平上丰富度分布

Figure 2. Relative abundance of microorganisms in Chinese narcissus rhizosphere soil at genus level

下载: 全尺寸图片幻灯片

表 1 中国水仙根际土壤微生物高通量测序数据及其多样性指数

Table 1. Summarized high-throughput sequences and diversity index of microorganisms in Chinese narcissus rhizosphere soil

微生物类型 Microorganism Type	测序序列数 Total sequence number	平均序列长度 Mean length/bp	OTUs （97%）	代表 OTUs	Chao 指数	Ace 指数	香农均匀度 Shannon evenness	辛普森均匀度 Simpson evenness	香农多样性指数 Shannon diversity index	辛普森多样性指数 Simpson diversity index
细菌 Bacteria	39 188	413.7	29 447	2 453	3 089	3 133	0.845 0	0.125 8	6.595 8	0.003 2
真菌 Fungi	66 216	381.8	49 162	109	109	109	0.523 8	0.056 6	2.455 1	0.162 0
古菌 Archaea	70 436	428.5	44 517	118	118	118	0.544 2	0.078 6	2.596 6	0.107 8

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