枯草芽孢杆菌悬浮种衣剂对辣椒幼苗生长和生理特性的影响

胡晨曦; 肖洒; 陈刚; 李子恒; 张云虹; 祁建波; 张瑛; 周如美; 张永泰; 张永吉

doi:10.19303/j.issn.1008-0384.2021.09.004

枯草芽孢杆菌悬浮种衣剂对辣椒幼苗生长和生理特性的影响

doi: 10.19303/j.issn.1008-0384.2021.09.004

胡晨曦^{1, 2,},
肖洒²,
陈刚¹,
李子恒^{1, 2},
张云虹¹,
祁建波²,
张瑛¹,
周如美¹,
张永泰¹,
张永吉^{1, 2, ,}

1.
江苏里下河地区农业科学研究所，江苏　扬州　225007
2.
扬州农科农业发展有限公司，江苏　扬州　225007

基金项目: 江苏省农业科技自主创新资金项目[CX（20）3177] ；江苏省蔬菜产业技术体系项目（JATS[2020]237）；扬州市“绿扬金凤计划”优秀博士项目（2019）；扬州市高效农业项目（扬农[2020]215号）

详细信息

作者简介:
胡晨曦（1992−），男，博士，助理研究员，研究方向：蔬菜栽培技术（E-mail：huchenxi345@126.com）

通讯作者:
张永吉（1985−），男，硕士，助理研究员，研究方向：蔬菜栽培技术（Email：zhangyongji85@126.com）

中图分类号: S 641
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- 文章访问数: 542
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- 被引次数: 0
出版历程
- 收稿日期: 2021-05-15
- 修回日期: 2021-08-24
- 网络出版日期: 2021-10-23
- 刊出日期: 2021-09-28

Effects of Seed-coating Bacillus subtilis Suspension on Growth and Physiology of Chili Pepper Seedlings

HU Chenxi^{1, 2
,},
XIAO Sa²,
Chen Gang¹,
LI Ziheng^{1, 2},
ZHANG Yunhong¹,
QI Jianbo²,
ZHANG Ying¹,
ZHOU Rumei¹,
ZHANG Yongtai¹,
ZHANG Yongji^{1, 2
, ,}

1.
Lixiahe Institute of Agricultural Sciences, Yangzhou, Jiangsu　225007, China
2.
Yangzhou Nongke Agricultural Development Co., Ltd., Yangzhou, Jiangsu　225007, China

摘要

摘要: 目的为在辣椒生产中合理利用枯草芽孢杆菌悬浮种衣剂提供理论依据。方法以扬椒2号为材料，研究了枯草芽孢杆菌悬浮种衣剂母液与种子不同药种比［1∶2、1∶4、1∶6、1∶8、1∶10和1∶12 （mL∶g）］对辣椒幼苗生长和生理特性的影响。结果与对照（不包衣）相比，种衣剂处理提高了辣椒幼苗出苗率、株高、茎粗、叶面积、植株干重和壮苗指数，且提高幅度随药种比的降低呈先上升后下降的趋势，以药种比1∶8的提高幅度最大。种衣剂处理提高了叶片净光合速率（Pn）、蒸腾速率（Tr）和气孔导度（Gs），降低了胞间CO₂浓度（Ci），增强了植株的光合能力。同时，种衣剂处理降低了叶片丙二醛（MDA）含量和超氧阴离子（O₂⁻）产生速率，提高了超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性，有利于维持叶片较高的叶绿素含量和可溶性蛋白含量。此外，相关分析表明，辣椒幼苗壮苗指数与叶片净光合速率、蒸腾速率和气孔导度呈显著正相关，与胞间CO₂浓度呈显著负相关。结论枯草芽孢杆菌悬浮种衣剂通过降低膜脂过氧化程度和提高抗氧化物酶活性来维持叶片较强的光合能力，从而有利于辣椒壮苗的形成，且以药种比1∶8的效果最佳。
- 枯草芽孢杆菌 /
- 悬浮种衣剂 /
- 药种比 /
- 辣椒幼苗 /
- 生长 /
- 生理特性
Abstract: Objective Applicability of a seed-coating Bacillus subtilis suspension for promoting chili pepper seedling growth was investigated. Method The chili pepper, Yangjiao No. 2, was used in the study by applying a B. subtilis agent on the seeds at the pharmacopoeia ratios of suspension to seeds at 1∶2, 1∶4, 1∶6, 1∶8, 1∶10, or 1∶12 (ml∶g). Subsequently, the seed germination as well as the seedling growth and physiological characteristics were monitored to determine the effects brought about by the coating treatments. Result Compared with control, the coating increased the seed germination rate and the plant height, stem diameter, leaf area, plant dry weight, and growth index of the seedlings. The effects peaked at the suspension to seeds ratio at 1∶8. The coating treatments enhanced the net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs) but reduced the intercellular CO₂ concentration (Ci) on the leaves resulting in an uplifted photosynthetic capacity of the plants. Moreover, the treatments decreased the MDA content and O₂⁻production rate, while increased the SOD, POD and CAT activities, of the leaves that were conducive to sustaining high contents of chlorophyll and soluble protein for the plants. The seedling growth index was found positively correlated with the net photosynthetic rate, transpiration rate, and stomatal conductance but inversely correlated with the intercellular CO₂ concentration. Conclusion The seed-coating B. subtilis suspension alleviated the peroxidation of membrane lipids and increased antioxidant enzyme activities with a sustained high photosynthetic capacity in the leaves benefiting a vigorous subsequent growth of the seedlings. The pharmacopoeia ratio at 1ml of suspension to 8g of seeds provided the most desirable results.
- Bacillus subtilis /
- seed-coating agent /
- pharmacopoeia ratio /
- chili pepper seedlings /
- plant growth /
- physiological characteristics

HTML全文

图 1 枯草芽孢杆菌悬浮种衣剂不同药种比对辣椒幼苗叶片光合参数的影响

注：同一图中不同小写字母表示处理间差异显著（P＜0.05），下同。

Figure 1. Effect of seed-coating applications on photosynthesis of chili pepper seedling leaves

Note: Data with different lowercase letters indicate significant differences at P＜0.05, same for below.

下载: 全尺寸图片幻灯片

图 2 枯草芽孢杆菌悬浮种衣剂不同药种比对辣椒幼苗叶片叶绿素含量和可溶性蛋白含量的影响

Figure 2. Effects of seed-coating applications on chlorophyll and soluble protein contents in chili pepper seedling leaves

下载: 全尺寸图片幻灯片

图 3 枯草芽孢杆菌悬浮种衣剂不同药种比对辣椒幼苗叶片丙二醛含量和超氧阴离子产生速率的影响

Figure 3. Effects of seed-coating applications on MDA content and O₂⁻production rate of chili pepper seedling leaves

下载: 全尺寸图片幻灯片

图 4 枯草芽孢杆菌悬浮种衣剂不同药种比对辣椒幼苗叶片超氧化物歧化酶、过氧化物酶和过氧化氢酶活性的影响

Figure 4. Effects of seed-coating applications on SOD, POD, and CAT activities of chili pepper seedling leaves

下载: 全尺寸图片幻灯片

图 5 枯草芽孢杆菌悬浮种衣剂不同药种比处理下辣椒幼苗壮苗指数与光合参数间的相关性分析

注：**表示在P＜0.01水平上显著，n=21。

Figure 5. Correlation between seedling growth index and photosynthetic parameters of chili pepper seedlings under varied seed-coating applications

Note: ** indicates extremely significant at P＜0.01 (n=21).

下载: 全尺寸图片幻灯片

表 1 枯草芽孢杆菌悬浮种衣剂不同药种比对辣椒幼苗生长的影响

Table 1. Effect of seed-coating applications on growth of chili pepper seedlings

处理 Treatment	出苗率 Germination rate/ %	株高 Plant height/ cm	茎粗 Stem diameter/ mm	叶面积 Leaf area/ cm²	植株干重 Plant dry weight/ mg	地上部干重 Aboveground dry weight/ mg	地下部干重 Underground dry weight/ mg	根冠比 Root shoot ratio	壮苗指数 Seedling index
CK	82.67 b	6.80 b	1.27 c	5.75 c	283.65 d	249.11 c	34.54 d	0.138 b	0.0446 d
T1	85.33 ab	6.92 b	1.30 bc	5.99 bc	291.90 cd	254.94 bc	36.95 cd	0.145 ab	0.0478 cd
T2	86.67 ab	7.19 ab	1.31 bc	6.26 abc	295.46 cd	257.08 bc	38.37 bc	0.149 ab	0.0490 bc
T3	87.33 a	7.31 ab	1.34 abc	6.36 ab	309.44 bc	268.30 ab	41.13 ab	0.153 a	0.0531 ab
T4	90.00 a	7.60 a	1.42 a	6.61 a	328.65 a	284.04 a	44.60 a	0.157 a	0.0577 a
T5	89.33 a	7.52 a	1.40 ab	6.54 a	323.11 ab	279.87 a	43.23 a	0.154 a	0.0559 a
T6	88.00 a	7.16 ab	1.32 abc	6.22 abc	294.86 cd	256.45 bc	38.40 bc	0.150 ab	0.0496 bc
注：同列数据后不同小写字母表示处理间差异显著（P＜0.05）。 Note: Data with different lowercase letters on same column indicate significant differences at P＜0.05.

下载: 导出CSV

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