Biocontrol Effect of Brevibacillus brevis FJAT-0809-GLX against Lasiodiplodia theobromae on Longan
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摘要:
目的 探明短短芽胞杆菌FJAT-0809-GLX对龙眼焦腐病菌生长的抑制作用。 方法 采用抑菌圈法和抑菌率法测定短短芽胞杆菌对龙眼焦腐病菌的拮抗活性，观察其对龙眼焦腐病菌菌丝生长和形态的影响。采用龙眼果实回接法，测定短短芽胞杆菌FJAT-0809-GLX对龙眼焦腐病菌的生防效果，并分析其对龙眼果皮防御酶活性的影响。 结果 短短芽胞杆菌FJAT-0809-GLX发酵上清液对龙眼焦腐病菌菌丝生长具有抑制作用，抑菌圈直径为15.77 mm。随着菌株培养时间的延长和添加量的增多，其对龙眼焦腐病菌生长的抑制效果也逐渐增强，添加50 mL时，抑菌率可达74.17%。接种龙眼焦腐病菌3 d后，好果率为68.33%，且龙眼果皮SOD酶和CAT酶活性均明显高于对照。 结论 短短芽胞杆菌FJAT-0809-GLX对龙眼焦腐病菌生长具有较强的抑制作用，在开发龙眼病害生物防治菌株方面具有一定的潜力。 Abstract:Objective Inhibitory effect of a strain of Brevibacillus brevis against Lasiodiplodia theobromae (Pat.) Griffon & Maubl on longan fruits was evaluated. Method In vitro antagonistic activity of B. brevis FJAT-0809-GLX was observed by the inhibition zone and rate on the mycelial growth and morphology of L. theobromae. Efficacy of the strain for biocontrol on the pathogen was determined by in vivo experiments, as well as the activities of defense enzymes in longan peel. Result Supernatant of the fermentation broth showed a pathogenic inhibition zone diameter of 15.77 mm and an inhibition rate that increased with increased fermentation time or treatment dosage. The inhibition rates reached 74.17% when 50 mL of the supernatant was applied. The supernatant inoculation on longan fruits significantly reduced the disease spreading rendering a healthy fruit rate of 68.33%. Meanwhile, the activities of superoxide dismutase and hydrogen peroxidase in the treated fruits became significantly higher than those of control. Conclusion B. brevis FJAT-0809-GLX significantly inhibited the growth of L. theobromae as shown by in vitro plating as well as in the field challenge test. It demonstrated a potential venue for bio-controlling the disease on longan. -
图 1 发酵上清液和菌体对龙眼焦腐病菌的抑菌圈
注：1：菌体；2：发酵上清液。
Figure 1. L. theobromae inhibition zone shown on medium by fermentation supernatant or bacteria cells
Note: 1: B. brevis FJAT-0809-GLX cells; 2: fermentation supernatant.
图 2 不同培养时间发酵上清液对龙眼焦腐病菌的抑菌圈
Figure 2. L. theobromae inhibition zones on medium treated with supernatants of varied fermentation time
图 3 不同添加量上清液对龙眼焦腐病菌生长的抑制效果
Figure 3. Inhibition of fermentation supernatant in varied dosages on growth of L. theobromae
图 4 短短芽胞杆菌FJAT-0809-GLX对龙眼焦腐病菌菌丝形态的影响
Figure 4. L. theobromae mycelial growth on medium with B. brevis FJAT-0809-GLX
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