Fluorescence Quenching on PEG400-modified ZnS:Cu Quantum Dots and Herbicide Diquat
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摘要:
目的 为探讨农药敌草快的快速检测方法，采用水热法制备水溶性PEG400修饰的ZnS：Cu量子点，通过荧光猝灭强度进行量子点与敌草快的互作信号表征。 方法 用荧光分光光度计、傅里叶红外光谱仪及紫外-可见分光光度计对合成的复合量子点进行表征，探究PEG400的修饰量对量子点的影响，同时测定量子点对细菌的抑制作用及毒性。 结果 在适宜的反应条件下，敌草快浓度为1.45×10−6～8.7×10−6 mol·L−1时，量子点荧光猝灭程度与敌草快的浓度呈现较好的线性关系，检出限为2.071×10−7 mol·L−1，相关系数R2达0.9999，是生物相容性好的低毒材料。 结论 所获得的PEG400修饰ZnS：Cu量子点初步可应用于农药敌草快的快速检测中，为相关检测技术的发展奠定基础。 Abstract:Objective Water-soluble PEG400-modified ZnS:Cu quantum dots (QDs) were prepared by hydrothermal method in search for an applicable rapid detection method on diquat. Method The QDs were characterized by their quenching intensities using a fluorescence spectrophotometer, an FTIR, and a UV-Vis spectrophotometer. Effect of PEG400 on the ZnS:Cu QDs was examined to determine the optimal modification for the establishment of a methodology to rapidly detect the toxicity of diquat on microorganisms. The correlation between QD quenching intensity and diquat concentration was examined for the methodology development. Result Under appropriate reaction conditions at the diquat concentration in the range of 1.45×10−6−8.7×10−6mol·L−1, the fluorescence quenching degree of QDs had an excellent linear relationship with diquat concentration of a correlation coefficient of 0.999 9. The detection limit of the method was 2.071×10−7mol·L−1. Conclusion The obtained PEG400-modified ZnS:Cu QDs was considered adequate to be applied for rapid detection of diquat in support of further development of related analytical methodology. -
Key words:
- ZnS: Cu quantum dots /
- PEG400 modification /
- fluorescence quenching /
- diquat
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图 1 ZnS:Cu量子点、不同含量PEG-ZnS:Cu量子点荧光激发光谱
Figure 1. Fluorescence excitation spectra of ZnS: Cu QDs and QDs containing varied amounts of PEG
图 2 ZnS:Cu量子点与不同含量PEG400修饰的PEG-ZnS:Cu量子点的红外光谱
Figure 2. Infrared spectra of ZnS:Cu QDs and QDs modified with varied amounts of PEG400
图 3 ZnS:Cu量子点与不同含量PEG400修饰的PEG-ZnS:Cu量子点的紫外光谱
Figure 3. UV spectra of ZnS:Cu quantum dots and QDs modified with varied amounts of PEG400
图 4 敌草快浓度对量子点的荧光猝灭光谱图
注：a-j敌草快浓度分别为： 0、1.45×10−6、2.9×10−6、4.35×10−6、5.8×10−6、7.25×10−6、8.7×10−6、10.15×10−6、11.6×10−6、13.05×10−6mol·L−1。
Figure 4. Fluorescence quenching spectra of QDs with varied diquat concentrations
Note: a-j: diquat concentrations at 0, 1.45×10−6, 2.9×10−6, 4.35×10−6, 5.8×10−6, 7.25×10−6, 8.7×10−6, 10.15×10−6, 11.6×10−6, and 13.05×10−6mol·L−1, respectively.
图 5 荧光强度与敌草快浓度的Stern-Volmer关系方程
Figure 5. Stern-Volmer equation on relationship between fluorescence intensity and diquat concentration
图 6 反应时间对量子点的荧光猝灭的光谱图
Figure 6. Spectrogram of fluorescence quenching of QDs by reaction time
表 1 量子点对大肠杆菌生长的平板计数测定
Table 1. Plate counts of Escherichia coli by QDs
平板序号
Plate
number单菌落数
Colony
numbers平板序号
Plate
number单菌落数
Colony
numbers抑菌率
Inhibitory
rate/%a（−） 292 e（+） 221 24.31 b（−） 274 f（+） 235 14.23 c（−） 260 g（+） 236 9.23 均值 Average 275 均值 Average 230 16.36 注：（−）表示未加入量子点；（+）表示加入量子点。
Note: (−): no added QDs; (+): with added QDs.
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