优质高产抗病耐储藏杂交稻品种内10优702的创制

陈丽萍; 谢鸿光; 蒋家焕; 王颖姮; 朱永生; 郑燕梅; 林强; 肖晏嘉; 蔡秋华; 吴方喜; 谢华安; 张建福

doi:10.19303/j.issn.1008-0384.2022.005.001

优质高产抗病耐储藏杂交稻品种内10优702的创制

doi: 10.19303/j.issn.1008-0384.2022.005.001

陈丽萍^{1, 2, 3,},
谢鸿光^{1, 2, 3},
蒋家焕^{1, 2, 3},
王颖姮^{1, 2, 3},
朱永生^{1, 2, 3},
郑燕梅^{1, 2, 3},
林强^{1, 2, 3},
肖晏嘉^{1, 2, 3},
蔡秋华^{1, 2, 3},
吴方喜^{1, 2, 3},
谢华安^{1, 2, 3, ,},
张建福^{1, 2, 3, ,}

1.
福建省农业科学院水稻研究所，福建　福州　350018
2.
农业农村部华南杂交水稻种质创新与分子育种重点实验室/福州（国家）水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/杂交水稻国家重点实验室华南研究基地，福建　福州　350003
3.
水稻国家工程研究中心，福建　福州　350003

基金项目: 国家水稻产业技术体系建设专项（CARS-01-20）；福建省农业高质量发展超越“5511”协同创新工程项目（XTCXGC2021001）；福建省科技重大专项（2020NZ08016）；福建省科技计划公益类专项（2019R1023-5）

详细信息

作者简介:
陈丽萍（1986−），女，博士，助理研究员，主要从事水稻分子育种研究（E-mail：373200630@qq.com）

通讯作者:
谢华安（1941−），男，研究员，主要从事杂交水稻育种研究（E-mail：huaanxie@163.com）

张建福（1971−），男，博士，研究员，主要从事水稻分子育种研究（E-mail：jianfzhang@163.com）

中图分类号: S 511
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出版历程
- 收稿日期: 2022-04-11
- 录用日期: 2022-04-11
- 修回日期: 2022-04-21
- 网络出版日期: 2022-05-21
- 刊出日期: 2022-05-28

Breeding of High-quality, High-yield, Blast-resistant, Long-shelf-life Hybrid Rice Nei-10-You-702

CHEN Liping^{1, 2, 3
,},
XIE Hongguang^{1, 2, 3},
JIANG Jiahuan^{1, 2, 3},
WANG Yingheng^{1, 2, 3},
ZHU Yongsheng^{1, 2, 3},
ZHENG Yanmei^{1, 2, 3},
LIN Qiang^{1, 2, 3},
XIAO Yanjia^{1, 2, 3},
CAI Qiuhua^{1, 2, 3},
WU Fangxi^{1, 2, 3},
XIE Huaan^{1, 2, 3
, ,},
ZHANG Jianfu^{1, 2, 3
, ,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fujian Academy of Agricultural Sciences/Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, China/South China Bases of National Key Laboratory of Hybrid Rice for China, Fuzhou, Fujian　350003, China
3.
National Rice Engineering Laboratory of China, Fuzhou, Fujian　350003, China

摘要

摘要: 目的创制生产上亟需的优质高产抗病耐储藏杂交稻新品种。方法将镇恢084、明恢86和多系1号通过多次杂交、回交、复交等育种技术，结合多年多品质鉴评、稻瘟病抗性鉴定及人工模拟老化进行耐储性鉴定等方法进行杂交稻新组合的选育。结果创制得到农艺性状优良的恢复系福恢702，将恢复系福恢702与优质三系不育系内香10A进行测交，选育出三系杂交稻新组合内10优702。内10优702的稻米品质达到部颁三级优质稻米质量标准，内10优702于2016−2017年参加湖北省恩施州区域试验及生产试验，两年区试每667 m²平均产量586.60 kg，比对照瑞优399增产3.51%。内10优702的田间自然发病鉴定及室内接菌鉴定都表现为抗稻瘟病，人工老化处理21 d，整体发芽率为97.33%，28 d后整体发芽率仍然高达73.33%，2019年8月通过湖北省农作物品种审定委员会审定（鄂审稻2019040）。结论内10优702是优质高产兼较强的稻瘟病抗性及耐储性的杂交稻新品种。
- 杂交稻品种 /
- 内10优702 /
- 优质 /
- 抗病 /
- 耐储藏
Abstract: Objectives A new hybrid rice variety of high quality, high yield, and blast resistance that produced grains with a long shelf life was bred to help boost the crop production. Methods A new restorer line derived from Zhenhui 084, Minghui 86, and Duoxi 1 by the conventional multiple crossing, backcrossing, and double crossing was subjected to a multi-point quality evaluation, blast resistance testing, and accelerated storage tests prior to final selection. Results The restorer line with desirable agronomic characteristics, Fuhui 702, was crossed with a new 3-line sterile line Neixiang 10A to obtain the hybrid Nei-10-You-702, which had all major physiochemical indices met the departmental quality standards of Grade III high-quality rice. It was, subsequently, submitted for testing at the regional trial and the production test at Enshi prefecture in Hubei Province from 2016 to 2017. It showed an average yield of 586.60 kg per 667 m², which was 3.51% higher than that of the reference Ruiyou 399. Nei-10-You-702 resisted rice blast according to a field observation as well as in an indoor challenge test. The seeds germinated at a rate of 97.33% after 21 d and 73.33% after 28 d of an artificial aging. It was approved by Hubei Provincial Crop Varieties Certification Committee in August 2019 (E Shen Dao 2019040). Conclusions The grains produced by the high-quality, high-yield, strongly blast-resistant Nei-10-You-702 could be stored for an extended period of time. It was deemed to become a promising new breed for rice production in the province.
- hybrid rice /
- Nei-10-You-702 /
- high quality /
- blast resistance /
- long shelf life

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图 1 福恢702和内10优702的创制过程

Figure 1. Development of Fuhui 702 and Nei-10-You-702

下载: 全尺寸图片幻灯片

图 2 香味基因Badh2在各品种中的单倍型分析

Figure 2. Haplotype analysis on fragrance gene Badh2 in various varieties

下载: 全尺寸图片幻灯片

图 3 内10优702、内香10A和福恢702的室内苗期稻瘟病抗性鉴定

①图中叶片为幼苗在室内接种菌株S2：NH17026后7 d的表型。② 1，云引；2，丽江新团黑谷；3，内香10A；4，内10优702；5，福恢702。

Figure 3. Phenotypes of Fuhui 702, Neixiang 10A, and Nei-10-You-702 inoculated for 7 d at seedling stage

① Image of leaf on seedling inoculated in laboratory by strain S₂: NH17026 for 7 d. ② 1: Yunyin; 2: Lijiangxintuanheigu; 3: Neixiang 10A; 4: Nei-10-You-702; 5: Fuhui 702.

下载: 全尺寸图片幻灯片

图 4 人工老化处理前后各品种的短时发芽率和整体发芽率

0 d：未处理的种子；21 d：人工老化处理21 d的种子；28 d：人工老化处理28 d的种子。同一图中误差线上方不同小写字母表示数据存在5%显著差异（P＜0.05）。

Figure 4. Percentages of shortened and overall seed germination rates of different varieties of rice before and after artificial aging

0 d: seeds before treatment; 21 d: seeds after a 21 d artificial aging; 28 d: seeds after a 28 d artificial aging. Data with different letters above error line indicate significant difference at P＜0.05.

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表 1 福恢702和内10优702的农艺性状及粒形比较

Table 1. Agronomic characteristics and grain appearances of Fuhui 702 and Nei-10-You-702

供试材料 Variety	株高Plant hight/cm	每丛有效穗数 Tiller number	穗长 Panicle length/cm	一次枝梗数 Primary branch	二次枝梗数 Secondary panicle branch	穗粒数 Total grain number per panicle	结实率Seed setting rate/%	粒长Grain lengt/mm	粒宽Grain width /mm	长宽比 Length- width ratio	千粒重1000- grain weight/g
福恢702 Fuhui 702	139.8	7.8	26.7	12.6	53.6	279.0	94.39	9.25	2.58	3.7	30.33
内10优 702 Nei-10-You-702	120.8^**	8.8^*	28.0^*	12.3	50.3^*	253.3^**	95.00	9.46^**	2.47^*	3.9^*	27.11^**
^表示在0.05水平上差异显著，^*表示在0.01水平上差异极显著。
^* represents significant difference at P＜0.05, ^** significant difference at P＜0.01.

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表 2 内10优702、内香10A和福恢702的稻瘟病抗性鉴定结果

Table 2. Blast resistance of Nei-10-You-702, Neixiang 10A, and Fuhui 702

品种 Variety	田间自然发病鉴定 Natural induction in field				室内苗瘟鉴定 Seedling blast
	苗瘟 Seedling blast	叶瘟 Leaf blast	穗颈瘟 Panicle blast	综合抗性 Evaluation of resistance	混合接菌 Mixed Inoculation	菌株 Strain										抗菌株率 Resistance frequency/%	综合抗性 Evaluation of resistance
	苗瘟 Seedling blast	叶瘟 Leaf blast	穗颈瘟 Panicle blast	综合抗性 Evaluation of resistance	混合接菌 Mixed Inoculation	S₁	S₂	S₃	S₄	S₅	S₆	S₇	S₈	S₉	S₁₀	抗菌株率 Resistance frequency/%	综合抗性 Evaluation of resistance
福恢702 Fuhui 702	R	MR	R	MR	S	R	S	S	S	S	MR	MR	S	S	R	30.77	S
内香10A Neixiang 10A	R	R	R	R	R	R	R	R	MR	R	R	MR	R	MS	R	92.31	R
内10优702 Nei-10-You-702	R	R	R	R	R	MR	R	R	R	R	R	R	MR	MR	R	100.00	R
R：抗；MR：中抗；MS：中感；S：感。 R: resistant; MR: moderately resistant; MS: moderately susceptible; S: susceptible.

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优质高产抗病耐储藏杂交稻品种内10优702的创制

doi: 10.19303/j.issn.1008-0384.2022.005.001

作者简介:
陈丽萍（1986−），女，博士，助理研究员，主要从事水稻分子育种研究（E-mail：373200630@qq.com）

通讯作者:
谢华安（1941−），男，研究员，主要从事杂交水稻育种研究（E-mail：huaanxie@163.com）

张建福（1971−），男，博士，研究员，主要从事水稻分子育种研究（E-mail：jianfzhang@163.com）

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Breeding of High-quality, High-yield, Blast-resistant, Long-shelf-life Hybrid Rice Nei-10-You-702

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目录

留言板

优质高产抗病耐储藏杂交稻品种内10优702的创制

doi: 10.19303/j.issn.1008-0384.2022.005.001

作者简介: 陈丽萍（1986−），女，博士，助理研究员，主要从事水稻分子育种研究（E-mail：373200630@qq.com）

通讯作者: 谢华安（1941−），男，研究员，主要从事杂交水稻育种研究（E-mail：huaanxie@163.com） 张建福（1971−），男，博士，研究员，主要从事水稻分子育种研究（E-mail：jianfzhang@163.com）

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Breeding of High-quality, High-yield, Blast-resistant, Long-shelf-life Hybrid Rice Nei-10-You-702

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出版历程

目录

作者简介:
陈丽萍（1986−），女，博士，助理研究员，主要从事水稻分子育种研究（E-mail：373200630@qq.com）

通讯作者:
谢华安（1941−），男，研究员，主要从事杂交水稻育种研究（E-mail：huaanxie@163.com）

张建福（1971−），男，博士，研究员，主要从事水稻分子育种研究（E-mail：jianfzhang@163.com）