不同施氮水平下AM真菌对高粱生物量及氮磷吸收的交互效应

王健; 张海欧; 杨晨曦; 李娟

doi:10.19303/j.issn.1008-0384.2022.007.003

不同施氮水平下AM真菌对高粱生物量及氮磷吸收的交互效应

doi: 10.19303/j.issn.1008-0384.2022.007.003

王健^{1, 2,},
张海欧^{1, 2},
杨晨曦^{1, 2},
李娟^{1, 2, ,}

1.
自然资源部退化及未利用土地整治重点实验室，陕西　西安　710075
2.
陕西地建土地工程技术研究院有限责任公司，陕西　西安　710075

基金项目: 陕西省创新人才推进计划——青年科技新星项目（2021KJXX-88）；陕西省科协企业创新争先青年人才托举计划项目（2021-1-2）；陕西省土地工程建设集团内部科研项目（DJNY2022-17）

详细信息

作者简介:
王健（1991−），男，硕士，助理工程师，主要从事土地整治技术研究（E-mail：WangJian_soil@163.com）

通讯作者:
李娟（1987−），女，高级工程师，主要从事土地整治相关研究工作（E-mail: 574435401@qq.com）

中图分类号: S 144.9
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出版历程
- 收稿日期: 2021-09-26
- 修回日期: 2021-12-19
- 网络出版日期: 2022-08-07
- 刊出日期: 2022-07-28

Effects of Interactions between AM Fungi and Nitrogen in Soil on Biomass and N-P Uptake of Sorghum Plants

WANG Jian^{1, 2
,},
ZHANG Haiou^{1, 2},
YANG Chenxi^{1, 2},
LI Juan^{1, 2
, ,}

1.
Key Laboratory of Degraded and Unused Land Consolidation Engineering, Ministry of Nature and Resources, Xi’an, Shaanxi　710075, China
2.
Institute of Land Engineering and Technology, Shaanxi Provincial Land Engineering Construction Group Co., Ltd., Xi’an, Shaanxi　710075, China

摘要

摘要: 目的氮沉降是影响陆地生态系统稳定的主要胁迫之一。研究氮沉降背景下丛枝菌根（Arbuscular mycorrhizal，AM）真菌对植物的生长影响，为全球气候变化背景下AM真菌生理生态学研究提供科学依据。方法采用完全随机设计，利用盆栽试验对高粱（Sorghum hicolor L. Mocrnch）幼苗设置接种AM（Glomus mosseae）菌剂和AM灭活菌剂（对照），氮水平处理包括0 mg·kg⁻¹（N0）、200 mg·kg⁻¹（N1）、400 mg·kg⁻¹（N2）、500 mg·kg⁻¹（N3）的NH₄NO₃。生长2周后进行菌根侵染率测定，在高粱植物完成生活史（16周）后进行植株生物量及氮磷含量的测定。结果 ①接种AM真菌显著提高了高粱根系的菌根侵染率（P＜0.001），且随着氮添加浓度的增加，菌根侵染率逐渐降低。②在未施氮处理（N0）中，接种AM真菌显著促进了高粱地上生物量及总生物量（P＜0.05），而在高浓度氮添加（N3）下，接种AM真菌显著抑制了高粱地上生物量及总生物量（P＜0.05）。③在未施氮处理（N0）中，接种AM真菌显著促进了高粱植物的氮、磷含量及组织氮磷比（P＜ 0.05），而在N2和N3氮水平下，接种AM真菌显著抑制了高粱组织的氮、磷含量及组织氮磷比（P＜0.05），尽管磷含量在N2水平无显著差异（P＞0.05）。④高粱的菌根生长效应（MGR）、菌根氮吸收效应（MNR）及菌根磷吸收效应（MPR）均随着氮梯度的增加逐渐由正效应转为负效应。结论 AM真菌接种和氮添加对高粱生物量及组织氮磷吸收存在显著的交互效应。AM真菌在低氮水平下有利于高粱生物量及组织氮磷含量的增加，随着氮添加量的不断增加，菌根效应逐渐由正效应转为负效应，说明在高氮水平下AM真菌不利于高粱生长。
- AM真菌 /
- 氮沉降 /
- 高粱 /
- 生物量 /
- 氮磷吸收 /
- 菌根效应
Abstract: Objective Effects of the widely distributed, important plant growth and stress resistance regulating arbuscular mycorrhizal (AM) fungi on sorghum growth under varied nitrogen (N) deposition in soil were studied on the terrestrial ecosystem. Method Growth of Sorghum hicolor (L.) Mocrnch seedlings inoculated with either Glomus mosseae or inactivated G. mosseae (CK) in pots under varied N addition of 0, 200, 400, or 500 mg·kg^-1 (refer to as N0, N1, N2, and N3, respectively) in a greenhouse was monitored. Mycorrhizal colonization in rhizosphere soils was determined after two weeks, while the biomass, N, and P of the plants in 16 weeks of cultivation. Result ① The AM fungi inoculation significantly increased the mycorrhizal colonization on sorghum roots (P＜0.001) at a decreasing trend with increasing N addition. ② Both the aboveground and total biomasses of the sorghum plants grown under N0 were significantly enhanced by the presence of the AM fungi but inhibited by it under N3 (P＜0.05). ③ Similarly, AM fungi increased the N and P contents and N/P ratio in the plant tissues by N0 but did the opposite under either N2 or N3 (P＜0.05). However, under N2 the AM fungal inoculation exerted no significant effect on P content (P＞0.05). And ④ the mycorrhizal growth effect (MGR), mycorrhizal N-uptake effect (MNR), and mycorrhizal P-uptake effect (MPR) on sorghum changed from positive to negative gradually as the N addition increased. Conclusion Inoculating AM fungi on sorghum seedlings and adding N in soil interactively affected the biomass and N-P uptake of the plants significantly. Without N addition, the AM fungi enhanced the accumulation of biomass, N, and P, but N addition maximized the mycorrhizal effect at certain level. High levels of N in soil could be detrimental to the growth of AM fungi and altered the symbiosis.
- AM fungi /
- nitrogen deposition /
- Sorghum hicolor (L.) Mocrnch /
- biomass /
- N and P uptake /
- mycorrhizal response

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图 1 不同氮水平下接种AM真菌对高粱生物量的影响

ns，P ＞0.05；* ，P ＜0.05；**，P ＜0.01；*** P ＜0.001。红星表示在同一个氮梯度下接种和不接种AM真菌之间差异达到显著水平(P ≤ 0.05)。图2同。

Figure 1. Effect of AM fungi inoculation on sorghum biomass with varied N deposition in soil

ns: significant difference (P>0.05), *: significant difference at P<0.05, **: significant difference at P <0.01, ***: significant difference at P <0.001. Red stars indicate significant differences on biomass of sorghum with or without AM fungi inoculation that grew on soil of same N level at P≤0.05. Same for Fig.2.

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图 2 不同氮水平下接种AM真菌对高粱组织氮含量的影响

Figure 2. Effect of AM fungi inoculation on tissue N concentrations of sorghum with varied N deposition in soil

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图 3 不同氮水平下高粱的菌根生长效应（a）、菌根氮吸收效应（b）和菌根磷吸收效应（c）

*表示菌根效应差异达到显著水平（P≤0.05）；红实线代表菌根效应原始变量和氮添加梯度之间线性回归拟合直线（P≤0.05）。

Figure 3. MGR (a), MNR (b), and MPR (c) of sorghum plants grown on soils with varied N deposition

* indicates significance at P≤0.05; red solid line represents regression between mycorrhizal effect and N addition at P≤0.05.

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表 1 不同处理下高粱菌根侵染状况

Table 1. AM fungi colonization on inoculated sorghum plants grown on soil with varied N deposition

氮添加 Nitrogen addition	AMF (+/−)	菌根侵染率 Mycorrhiza colonization/ %	丛枝侵染率 Arbuscular colonization/ %	泡囊侵染率 Vesicles colonization/ %
N0	+	57.15 ± 1.70 a	13.25 ± 0.89	23.86 ± 1.45 a
N1	+	44.01 ± 1.89 b	11.52 ± 1.33	24.10 ± 1.40 a
N2	+	31.71 ± 3.55 c	9.14 ± 1.28	17.50 ± 0.57 b
N3	+	33.31 ± 0.67 c	10.25 ± 1.05	19.72 ± 1.97 b
N0	−	0 ± 0	0 ± 0	0 ± 0
N1	−	0 ± 0	0 ± 0	0 ± 0
N2	−	0 ± 0	0 ± 0	0 ± 0
N3	−	0 ± 0	0 ± 0	0 ± 0
处理效应 Treatment effect
氮添加 N addition	F值F value	44.57	1.47	19.24
氮添加 N addition	P值P value	＜0.001	0.237	＜0.001
真菌接种 AM colonization	F值F value	3101.01	235.49	259.13
真菌接种 AM colonization	P值P value	＜0.001	＜0.001	＜0.001
表中数据为均值±标准误；数据后不同小写字母表示不同氮添加量处理之间差异显著（P＜0.05）。 Data are mean ± standard error; different letters represent significant differences between groups (P＜0.05).

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