48份观赏海棠种质不同叶位叶片色彩组分及分类研究

王静茹; 张往祥; 狄昌毅; 陆晓吉

doi:10.19303/j.issn.1008-0384.2022.009.007

48份观赏海棠种质不同叶位叶片色彩组分及分类研究

doi: 10.19303/j.issn.1008-0384.2022.009.007

王静茹^1,,
张往祥^{1, 2, ,},
狄昌毅¹,
陆晓吉¹

1.
南京林业大学林学院，江苏　南京　210037
2.
扬州小苹果园艺有限公司，江苏　扬州　225200

基金项目: 江苏省科技计划重点项目（BE2019389）

详细信息

作者简介:
王静茹（1997−），女，硕士研究生，研究方向：观赏植物应用（E-mail：1453160171@qq.com）

通讯作者:
张往祥（1965−），男，博士，教授，研究方向：观赏植物应用（E-mail：malus2011@163.com）

中图分类号: S 685.99
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- 被引次数: 0
出版历程
- 收稿日期: 2022-06-01
- 修回日期: 2022-06-24
- 网络出版日期: 2022-10-21
- 刊出日期: 2022-09-30

Leaf Color and Pigments of 48 Ornamental Crabapple Germplasms Leaves

1.
College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu　210037, China
2.
Yangzhou Crabapple Horticulture Co., Ltd., Yangzhou, Jiangsu　225200, China

摘要

摘要: 目的探究48份观赏海棠种质不同叶位的叶片在观赏期内的色彩与色素组分变化规律，对主要观赏叶位的叶片色彩参数进行分类研究，建立观赏海棠叶色评价体系。方法以48份观赏海棠种质为试验材料，使用色差仪和光谱仪对不同品种的上、中、下位叶进行色彩参数和色素含量的测定。结果（1）基于6月份不同叶位色彩参数的亮度值（L*）、饱和度（C*）、色调角（h°）在CIELCH色空间中的分布以及不同叶位的叶色频率分布图分析，所测观赏海棠品种的叶片位点随着叶位下降呈现逐渐集中趋势，上位叶色彩表现最为丰富，位点最为分散。（2）色素相对含量的分析结果表明48份观赏海棠种质叶片中的花青素（Anthocyanin）、类胡萝卜素（Carotenoid）相对含量位点随着叶位下降整体呈左移（减小）的趋势，叶片中叶绿素（Chlorophyll）相对含量的位点随着叶位下降整体呈上移（增大）趋势。（3）叶色聚类分析将48个观赏海棠种质划分为绿色类群A（亮绿色系A₁、深绿色系A₂）、棕色类群B和红色类群C（红棕色系C₁、紫红色系C₂）3大类群。结论 A₁色系叶绿素相对含量高，叶片色彩亮度高，颜色饱满，为优良的亮绿色观叶品种；B类群各色彩参数与色素相对含量差异性显著，类胡萝卜素相对含量显著高于其他类群，可作为进行叶色改良的试验品种；C₁色系花青素与叶绿素相对含量的比值高（Anth/Chl=1.22±0.24），色调角位于红色区域（h°=29.74°±4.74°），红色特性显著，是优良的红叶观赏品种。
- 观赏海棠 /
- 叶片色彩 /
- 色素相对含量 /
- 评价分析
Abstract: Objective Variations in color and pigments on leaves of ornamental crabapple germplasms in season were used to classify the plants for establishing an evaluation system for landscaping. Method Luminosity L*, saturation C*, and hue angle h° as well as pigments in the upper, middle, and lower parts of the leaf of 48 ornamental crabapple germplasms were measured using a colorimeter and a spectrometer. Results ① The color measurements on all crabapple varieties in June distributed in the CIELCH space more concentrated in the upper part and gradually lessened toward the lower part of a leaf. The color was richer and the loci more scattered in the upper part as well. ② The relative contents of anthocyanin and carotenoid decreased, but that of chlorophyll increased, from the upper to the lower leaf. ③ The germplasms clustered by leaf color were classified into the green Group A that included bright green A₁ and dark green A₂, the brown Group B, and the red Group C that included reddish brown C₁ and reddish purple C₂. Conclusion Of all crabapple groups, Group A₁ had a high relative chlorophyll content displaying bright green foliage; Group B had significantly varied color measurements and a significantly higher carotenoids content than the other groups making it suitable for breeding programs; and Group C₁ had a high anthocyanin to chlorophyll ratio of （1.22±0.24） and an h° of (29.74±4.74)° rendering it the choice of a showy red-leaf crabapple variety.
- Ornamental crabapple /
- leaf color /
- relative pigment content /
- evaluation and analysis

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图 1 观赏海棠种质不同叶位叶片 CIELCH色空间变化

Figure 1. Color measurements on upper to lower leaf portions of ornamental crabapple germplasms as distributed in CIELCH space

下载: 全尺寸图片幻灯片

图 2 观赏海棠种质不同叶位叶色参数频率分布

Figure 2. Frequency distribution of leaf color measurements on upper to lower leaf of ornamental crabapple germplasms

下载: 全尺寸图片幻灯片

图 3 观赏海棠种质不同叶位色素相对含量与权重的空间分布

A为观赏海棠种质不同叶位色素相对含量的空间分布，B为观赏海棠种质不同叶位色素权重占比的空间分布。

Figure 3. Spatial distribution of relative content and weight of pigments in upper to lower leaf of ornamental crabapple germplasms

A shows the spatial distribution of relative pigment content in different leaf positions of ornamental crabapple germplasms, and B shows the spatial distribution of pigment weight ratio in different leaf positions of ornamental crabapple germplasms.

下载: 全尺寸图片幻灯片

图 4 观赏海棠上位叶叶色聚类分析

Figure 4. Cluster analysis on germplasms based on color of upper portion of an ornamental crabapple leaf

下载: 全尺寸图片幻灯片

表 1 48份观赏海棠种质种质信息

Table 1. Information on 48 sampled ornamental crabapple germplasms

序号 Code	种质名称 Cultivars	品种审定号 Variety validation number	序号 Code	种质名称 Cultivars	品种审定号 Variety validation number
1	琉璃盏 M. Liu Lizhan	20190169	25	白云 M. Bai Yun	20210210
2	红与黑 M. Hong Yuhei	20190170	26	橙之梦 M. Cheng Zhimeng	20190076
3	云卷云舒 M. Yunjuan Yunshu	20190361	27	茶花女 M. Cha Huanv	20210208
4	影红秀 M. Ying Hongxiu	20190171	28	二乔 M. Er Qiao	20210207
5	忆红莲 M. Yi Honglian	20190364	29	红晨 M. Hong Chen	20210206
6	疏红妆 M. Shu Hongzhuang	20190172	30	红粉佳人 M. Hongfen Jiaren	20210205
7	棱镜 M. Leng Jing	20190168	31	红色经典 M. Hongse Jingdian	20210204
8	白羽扇 M. Bai Yushan	20190173	32	红云 M. Hong Yun	20210203
9	涟漪 M. Lian Yi	20190167	33	浪花 M. Lang Hua	20210202
10	卷珠帘 M. Juan Zhulian	20190363	34	飘 M. Piao	20210209
11	依人 M. Yi Ren	20190365	35	水袖 M. Shui Xiu	20210201
12	洛可可女士 M. Luokeke Nvshi	20190080	36	西子姑娘 M. Xizi Guniang	20210200
13	胭脂雨 M. Yan Zhiyu	20180299	37	夏荷 M. Xia He	20210199
14	羊脂玉 M. Yang Zhiyu	20190078	38	紫嫣 M. Zi Yan	20210198
15	紫蝶儿 M. Zi Dieer	20190081	39	红颜 M. Hong Yan	20210411
16	红色依恋 M. Hongse Yilian	20180296	40	金秋 M. Jin Qiu	20210414
17	云想容 M. Yun Xiangrong	20190079	41	春花秋月 M. Chunhua Qiuyue	20210410
18	粉芭蕾 M. Fen Balei	20170082	42	满堂红 M. Man Tanghong	20170094
19	画轴 M. Hua Zhou	20180298	43	香荷 M. Xiang He	20170096
20	烟雨江南 M. Yanyu Jiangnan	20190366	44	香云 M. Xiang Yun	20160062
21	晚宴 M. Wan Yan	20180297	45	昌红 M. Chang Hong	20160061
22	粉红霓裳 M. Fenhong Nichang	20190077	46	昌辉 M. Chang Hui	20160058
23	红珊瑚 M. Hong Shanhu	20190367	47	丽辉 M. Li Hui	20160060
24	千层金 M. Qian Cengjin	20190362	48	蕊沁 M. Rui Qin	20160059

下载: 导出CSV

表 2 各类群色彩与色素参数统计

Table 2. Statistics on color measurements and pigment contents of each taxon

色系分类 Color category	种质数量 Quantity	色彩参数 Color parameters			色素含量 Pigment parameters
色系分类 Color category	种质数量 Quantity	L*	C*	h°/°	花青素Anth/%	类胡萝卜素Car/%	叶绿素Chl/%	花青素/叶绿素Anth/Chl
A绿色系 Green	32	37.89±5.71 ab	19.46±8.40 ab	115.23±7.73 ab	0.03±0.03 c	0.24±0.08 cd	0.73±0.09 a	0.04±0.06 c
A₁亮绿色系 Bright green	17	42.58±3.29 a	26.09±5.42 a	109.72±6.47 b	0.03±0.04 c	0.28±0.07 bc	0.68±0.09 a	0.06±0.07 c
A₂深绿色系 Dark green	15	32.58±1.74 bc	11.94±2.96 bc	121.47±2.42 a	0.03±0.02 c	0.19±0.06 d	0.78±0.07 a	0.04±0.03 c
B棕色系 Brown	3	36.78±0.44 b	16.63±3.25 bc	86.27±7.18 c	0.24±0.02 b	0.38±0.00 a	0.38±0.02 b	0.65±0.10 b
C 红色系 Red	13	31.13±3.27 c	9.51±2.32 c	35.18±11.41 e	0.34±0.03 a	0.37±0.04 ab	0.29±0.05 b	1.19±0.26 a
C₁红棕色系 Reddish brown	10	30.44±3.43 c	9.14±2.32 c	29.74±4.74 e	0.35±0.03 a	0.37±0.03 ab	0.29±0.03 b	1.22±0.24 a
C₂紫红色系 Red purple	3	33.44±0.80 bc	10.77±2.20 c	53.28±6.42 d	0.32±0.03 a	0.37±0.06 ab	0.31±0.08 b	1.09±0.34 a
数值表示为平均值±标准差；同列数据后无相同小写字母表示差异显著（P＜0.05）。 Data are presented as mean±standard deviation; data with different lowercase letters on same column indicate significant difference (P＜0.05).

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