甘薯基部腐烂病对植株根际土壤细菌群落结构的影响

陈海生; 唐超文; 刘守平; 陈伟强; 侯金祥

doi:10.19303/j.issn.1008-0384.2022.009.011

甘薯基部腐烂病对植株根际土壤细菌群落结构的影响

doi: 10.19303/j.issn.1008-0384.2022.009.011

1.
浙江同济科技职业学院，浙江　杭州　311231
2.
益阳职业技术学院，湖南　益阳　413000
3.
台州市农业科学研究院，浙江　临海　317000

基金项目: 浙江省科技厅项目（LGN18C030002）

详细信息

作者简介:
陈海生（1965−），男，博士，教授，研究方向：土壤微生物（E-mail：haishch@126.com）

通讯作者:
刘守平（1964−），男，研究员，研究方向：作物病害（E-mail：shpliu2005@126.com）

中图分类号: Q 939
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- 被引次数: 0
出版历程
- 收稿日期: 2022-04-12
- 修回日期: 2022-06-09
- 网络出版日期: 2022-10-05
- 刊出日期: 2022-09-30

Rhizosphere Microbial Community as Affected by Sweet Potato Stem Rot Disease

1.
Zhejiang Tongji Vocational College of Science and Technology, Hangzhou, Zhejiang　311231, China
2.
Yiyang Vocational and Technical College, Yiyang, Hunan　413000, China
3.
Taizhou Academy of Agricultural Sciences, Linhai, Zhejiang　317000, China

摘要

摘要: 目的探明甘薯茎基部腐烂病发生机制，为构建该病害的绿色防控体系提供依据。方法以处于南北薯区交接带、南北甘薯病害发生区的浙江省台州市黄岩区西部山区为研究对象，采用Illumina高通量测序技术，对比研究甘薯茎基部腐烂病发病甘薯植株与健康甘薯植株的根际土壤细菌群落结构和多样性，及其根际土壤酶包括土壤脲酶、蔗糖酶和碱性磷酸酶活性。结果甘薯茎基部腐烂病显著降低了植株根际土壤脲酶、蔗糖酶和碱性磷酸酶活性，提高了根际土壤细菌多样性指数。在门分类水平上，该病害显著降低了其植株根际土壤放线菌门、疣微菌门和拟杆菌门相对丰度，显著提高了绿湾菌门和Candidate-division-wps-2菌群的相对丰度。在属分类水平上，该病害显著提高了植株根际土壤细菌GP₁、未分类的厚壁菌属和芽单胞菌属的相对丰度，显著降低了未分类的链霉菌属、链嗜酸菌属、伯克霍尔德菌属等菌群的相对丰度。结论甘薯茎基部腐烂病降低了其植株根际脲酶、蔗糖酶和碱性磷酸酶活性，改变了甘薯根际土壤细菌群落的结构和多样性。
- 甘薯茎基部腐烂病 /
- 高通量测序 /
- 根际土壤细菌群落结构 /
- 土壤酶活性
Abstract: Objective Microbial communities in rhizosphere soils of healthy sweet potato plants and those infected by the stem rot disease (SPSR) were compared for ecological disease control. Method Rhizosphere soil samples at fields of healthy and SPSR-infected sweet potatoes in between north and south planting regions and disease occurring western hilly areas of Huangyan, Taizhou City, Zhejiang Province, were collected. Enzyme activity and microbial community in the soil were analyzed using the traditional and high throughput sequencing techniques. Results Compared with the rhizosphere soils of non-infected plants, those associated with the SPSR-infected sweet potatoes exhibited significant inhibition on the activities of urease, invertase, and alkaline phosphatase as well as significant increases on the microbial diversity and richness such as Chao1 and Shannon indices. At phylum level, the average relative abundance of dominant microbes, such as Actinobacteria, Verrucomicroba, and Bacteroidetes, were significantly lower in the SPSR soil, but that of Chloroflexi and Candidate-division-wps-2 significantly higher. At genus level, the average relative abundance of GP_₁, Firmicutes-unclassified, and Gemmatimonas was higher in the SPSR samples, while that of Streptomycetaceae-unclassified, Streptacidiphilus, and Burkhoideria lower. Conclusion In the rhizosphere soils of SPSR-infected sweet potato plants, the activities of some important soil enzymes were low. At both phylum and genus levels, the great variations in the abundance of dominant microorganisms existed between the healthy and SPSR rhizosphere soils.
- Stem base rot disease of sweet potato /
- high throughput sequencing /
- rhizosphere soil microbial community structure /
- soil enzyme activity

HTML全文

图 1 基于操作分类单元丰度的土壤细菌群落维恩图

Figure 1. Venn diagram of microbial communities in rhizosphere soil

下载: 全尺寸图片幻灯片

图 2 甘薯茎基部腐烂病发病与健康甘薯植株根际土壤细菌门水平群落结构

Figure 2. Compositions of microbial communities in rhizosphere soils of healthy and SPSR-infected sweet potato fields at phylum level

下载: 全尺寸图片幻灯片

图 3 甘薯茎基部腐烂病发病与健康植株根际土壤细菌属水平群落结构

Figure 3. Compositions of microbial communities in rhizosphere soils of healthy and SPSR-infected sweet potato fields at genus level

下载: 全尺寸图片幻灯片

表 1 甘薯茎基部腐烂病发病植株与健康植株根际土壤酶活性比较

Table 1. Enzyme activities in rhizosphere soils of healthy and SPSR-infected sweet potato fields

根际土壤 Rhizosphere soils	脲酶活性 Urease activity/ (mg·g⁻¹·d⁻¹)	蔗糖酶活性 Invertase activity/ (mg·g⁻¹·d⁻¹)	碱性磷酸酶活性 Alkaline phosephatase/ (µg·g⁻¹·d⁻¹)
发病植株 Infected	0.26±0.02 b	9.56±0.31 b	7.12±0.24 b
健康植株 Non-infected	0.48±0.06 a	24.17±0.19 a	12.89±0.63 a
同列数值后不同小写字母表示处理间差异在5%水平上显著。 Values followed by different small letters in the column are significantly different at 5% level.

下载: 导出CSV

表 2 发病与健康甘薯植株根际土壤细菌Alpha多样性

Table 2. Alpha diversity of microbial communities in rhizosphere soils of healthy and SPSR-infected sweet potato fields

根际土壤样品　　 Rhizosphere soils	物种数 Observed species	Chao1指数 Chao1 index	辛普森指数 Simpson index	香农指数 Shannon index	覆盖度 Coverage/%
发病植株 Infected	6 493.00 a	6906.24±11.02 a	0.98±0.01 a	10.78±0.03 a	98.35
健康植株 Non-infected	6 072.00 b	6625.01±13.11 b	0.97±0.03 a	10.50±0.05 a	98.61
同列不同小写字母表示差异显著（P＜0.05）。 Data with different lowercase letters on same column indicate significant difference at P＜0.05.

下载: 导出CSV

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