Biological Characteristics of Geotrichum candidum–Pathogen of Sour Rot Disease on Pseudostellaria heterophylla
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摘要:
目的 明确太子参酸腐病病原菌白地霉（Geotrichum candidum）FJAT-32652的生物学特性。 方法 测定白地霉在不同温度、pH值、光照、碳源和氮源培养条件下的菌落直径，以及孢子的致死温度和时长，并用显微镜观察孢子在不同培养时间的萌发情况。 结果 该病原菌生长最适温度为30 ℃，pH值为6，碳源为麦芽糖、乳糖和葡萄糖，氮源为硫酸铵，不同光照条件对 菌丝生长无显著影响，孢子的致死温度及时长为65 ℃水浴10 min。病原菌孢子培养3 h开始萌发，9 h形成菌丝，繁殖方式为裂殖。 结论 明确了太子参酸腐病病原菌白地霉FJAT-32652的生物学特性，为太子参酸腐病的后续研究提供基础数据。 Abstract:Objective Biological characteristics of the pathogen causing sour rot disease on Pseudostellaria heterophylla were studied. Method Diameters of Geotrichum candidum FJAT-32652 colonies on culture medium under varied temperature, pH, light, and carbon and nitrogen sources were measured to determine the optimal growth conditions for the pathogen. Lethal temperature and time of the spores were determined, and spore germination observed under a microscope. Results The optimum growth conditions for the pathogen included 30 ℃, pH 6, maltose, lactose, and glucose for carbon source, and ammonium sulfate for nitrogen source. Light exposure had no significant effect on the mycelial growth. Lethality occurred by submerging the spores in 65 ℃ hot water for 10 min. Spore germination started 3 h after incubation, and mycelia became visible in 9 h. Schizogamy was the reproduction mode of the pathogen. Conclusion The biological characteristics of G. candidum were determined for subsequent studies on the sour rot disease of P. heterophylla. -
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