不同生境对紫两优737类黄酮及B族维生素含量的影响

房贤涛; 郑建华; 游晴如; 廖发炼; 董练飞; 王洪飞; 董瑞霞; 陈春霞; 黄庭旭

doi:10.19303/j.issn.1008-0384.2022.012.004

不同生境对紫两优737类黄酮及B族维生素含量的影响

doi: 10.19303/j.issn.1008-0384.2022.012.004

1.
福建省农业科学院水稻研究所/福州国家水稻改良分中心，福建　福州　350018
2.
福建省宁化县良种场，福建　宁化　365400
3.
福建省南平市建阳区农业农村局，福建　建阳　354200

基金项目: 福建省现代水稻产业技术体系建设专项（闽财指〔2021〕637号）；福建省科技计划公益类专项（2018R1021-6）

详细信息

作者简介:
房贤涛（1980−），男，硕士，助理研究员，研究方向：水稻遗传育种（E-mail：187182644@qq.com）

通讯作者:
黄庭旭（1964−），男，硕士，研究员，研究方向：水稻遗传育种（E-mail：610143535@qq.com）

中图分类号: S 511
计量
- 文章访问数: 317
- HTML全文浏览量: 135
- PDF下载量: 63
- 被引次数: 0
出版历程
- 收稿日期: 2022-06-20
- 录用日期: 2022-06-20
- 修回日期: 2022-11-02
- 网络出版日期: 2023-01-01
- 刊出日期: 2022-03-28

Effects of Habitats on Flavonoids and Vitamin B Contents in Ziliangyou 737

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences/Fuzhou Branch, National Rice Improvement Center of China, Fuzhou, Fujian　350018, China
2.
Ninghua County Seed Multiplication Farm of Fujian, Ninghua, Fujian　365400, China
3.
Agricultural and Rural Bureau of Jianyang District of Nanping City, Nanping, Fujian　354200, China

摘要

摘要: 目的探索紫黑米品种紫两优737在不同种植季节及不同生境的黄酮及Ｂ族维生素的变化差异，进而为生产者最佳种植季节及种植地点安排、提高紫两优737生产品质提供科学依据。方法收集2021年4月中旬至10月中旬种植于福建12个地方的紫两优737样本，测定其花青素、黄酮、类胡萝卜素和B族维生素含量，并进行主成分分析。结果晚季和烟后季种植情况下，紫两优737样品中花青素、黄酮和类胡萝卜素含量明显比早季和中季高，且随海拔升高，这3种物质都有上升的趋势；总维生素含量方面，早、晚季种植比中季和烟后季种植含量高，维生素含量与海拔无明显相关关系。主成分分析表明，VB₆、VB₄、VB₅、花青素、黄酮和类胡萝卜素在第一主成分上有较高的载荷，这一部分体现了紫黑米中主要的营养物质。VB₃、VB₈、VB₇在第二主成分上有较高载荷，VB₁和VB₂则在第三主成分上有较高载荷。样品中得分最高的是宁德岚亭晚季样本，中季样本普遍得分较低。结论紫两优737的 B族维生素含量VB₅、VB₁、VB₆较高；从种植季节或种植地点而言，晚季种植或光照充足、气温较低的高海拔地区种植的样本均含有较高花青素、黄酮、类胡萝卜素和B族维生素。
- 紫两优737 /
- 花青素 /
- 黄酮 /
- 类胡萝卜素 /
- B族维生素
Abstract: Objective Effects of planting season and location on the flavonoids and B vitamins contents in the purple black rice Ziliangyou 737 were studied. Method Contents of anthocyanins, flavonoids, carotenoids, and B vitamins of the rice specimens collected from 12 localities in Fujian in mid-April to mid-October 2021 were determined and statistically analyzed for principal components. Result The contents of anthocyanins, flavonoids, and carotenoids in grains were significantly higher in the rice planted in the late or post-tobacco season than in the early or middle season. The contents also increased with increasing altitude of the area where the rice was cultivated. On the other hand, vitamins were more abundantly found in the rice planted in the early or late season than in the middle or post-tobacco season, but not significantly affected by elevation. VB₆, VB₄, VB₅, anthocyanin, flavonoid, and carotenoids represented a greater load as the first principal components and were the major nutrients in the rice. The 2^nd principal components included VB₃, VB₈, and VB₇, while the 3^rd principal components, VB₁ and VB₂. Overall, the rice planted in the late season from Lanting, Ningde received the highest score among all, while the mid-season rice tended to be low. Conclusion On B vitamins, Ziliangyou 737 contained more VB₅, VB₁, and VB₆ than the others. The rice planted in late season or in areas of high altitude with sufficient sunlight and low temperature appeared to have higher contents of anthocyanin, flavonoids, carotenoids, and B vitamins.
- Ziliangyou 737 /
- anthocyanins /
- flavonoids /
- carotenoid /
- B vitamins

HTML全文

表 1 各样本信息

Table 1. Information on specimens

产地 Origin	播种期 Seeding time/（月-日）	抽穗期 Period of heading/（月-日）	成熟期 Mature period/（月-日）	所属种植类型 Planting type	海拔 Elevation/m
漳州宝斗 Baodou, Zhangzhou	04-22	08-03	09-06	早 Early season	121
莆田南兴 Nanxing, Putian	05-01	08-20	09-30	中 Middle season	590
三明杨城 Yangcheng, Sanming	06-15	09-06	10-19	烟后 Post-tobacco season	380
南平后洋 Houyang, Nanping	05-18	08-19	09-26	中 Middle season	419
宁德岚亭 Lanting, Ningde	06-20	09-17	10-28	晚 Late season	20
宁德南湾 Nanwan, Ningde	05-08	08-25	10-06	中 Middle season	950
南平五夫 Wufu, Nanping	05-25	08-16	09-30	中 Middle season	230
南平横墩 Hengdun, Nanping	05-26	08-23	10-15	中 Middle season	380
南平吴边 Wubian, Nanping	05-18	08-26	10-05	中 Middle season	579
南平倪坜 Nili, Nanping	04-25	08-09	09-20	中 Middle season	938
泉州瑞坂 Ruiban, Quanzhou	05-25	08-28	10-08	中 Middle season	285
龙岩黄埔 Huangpu, Longyan	06-15	09-10	10-13	晚 Late season	242

下载: 导出CSV

表 2 紫两优737维生素含量随种植季节的变化分析

Table 2. Vitamin contents of grains from rice planted in different seasons

种植季节 Planting season	盐酸硫胺素 VB₁/ (ng∙g⁻¹)	核黄素 VB₂/ (ng∙g⁻¹)	烟酰胺 VB₃/ (ng∙g⁻¹)	腺嘌呤 VB₄/ (ng∙g⁻¹)	泛酸 VB₅/ (μg∙g⁻¹)	吡哆醇 VB₆/ (ng∙g⁻¹)	维生素 H VB₇/ (ng∙g⁻¹)	腺嘌呤核苷酸 VB₈/ (ng∙g⁻¹)	总维生素 ∑VB_i/ (μg∙g⁻¹)
早 Early season	485.81±3.10 b	350.20±4.31 a	592.81±7.72 a	319.91±7.32 b	9.47±0.01 a	394.41±3.12 a	2.31±1.04 a	0.92±0.20 c	11.62±0.12 a
中 Middle season	444.51±4.00 c	335.91±1.73 b	255.33±1.95 bc	303.01±3.21 c	7.93±0.02 c	355.92±1.63 c	1.43±0.23 a	10.72±1.22 b	9.64±0.01 d
烟后 Post-tobacco season	564.4±17.91 a	313.45±9.46 c	262.57±4.57 b	269.33±5.51 d	8.99±0.15 b	371.23±8.24 b	1.55±0.63 a	13.43±2.38 a	10.79±0.19 c
晚 Late season	557.52±7.23 a	350.16±4.65 a	250.74±4.43 c	634.12±12.82 a	8.97±0.03 b	399.52±8.25 a	1.72±0.24 a	2.69±0.86 c	11.17±0.03 b
最大值 Max.	598.41	392.42	598.53	688.43	9.85	478.93	3.52	31.05	12.01
最小值 Min.	353.52	300.73	218.71	190.35	6.24	237.23	0.92	0.63	7.64
平均数 Average	476.81	337.61	283.31	356.81	8.32	367.75	1.62	8.82	10.16
变异系数 CV/%	15.01	8.12	34.11	45.44	12.12	18.02	31.74	93.17	11.92
同列数据后不同小写字母表示差异显著（P＜0.05）,下同。 Different lowercase letters behind same column data indicate significant differences (P＜0.05). The same as below.

下载: 导出CSV

表 3 不同种植季节紫两优737花青素、黄酮、类胡萝卜素含量变化分析

Table 3. Anthocyanins, flavonoids, and carotenoid in grains from rice planted in different seasons

种植季节 Planting season	花青素 Anthocyanin/ (mg∙g⁻¹)	黄酮 Flavonoids/ (mg∙g⁻¹)	类胡萝卜素 Carotenoid/ (μg∙g⁻¹)
早 Early season	0.45±0.00 d	3.94±0.02 d	38.41±0.20 d
中 Middle season	1.35±0.01 c	4.39±0.02 c	69.32±0.30 c
烟后 Post-tobacco season	2.15±0.01 a	4.88±0.05 a	80.21±0.31 b
晚 Late season	2.10±0.02 b	4.79±0.06 b	83.14±0.30 a
最大值 Max.	2.19	5.67	96.60
最小值 Min.	0.45	3.36	38.31
平均数 Average	1.47	4.46	70.01
变异系数 CV/%	38.08	15.86	25.45

下载: 导出CSV

表 4 维生素含量随海拔变化的分析

Table 4. Vitamin contents in grains in relation to altitude of rice growing area

地点 Locations	海拔 Elevation/ m	盐酸硫胺素 VB₁/ (ng∙g⁻¹)	核黄素 VB₂/ (ng∙g⁻¹)	烟酰胺 VB₃/ (ng∙g⁻¹)	腺嘌呤 VB₄/ (ng∙g⁻¹)	泛酸 VB₅/ (μg∙g⁻¹)	吡哆醇 VB₆/ (ng∙g⁻¹)	维生素 H VB₇/ (ng∙g⁻¹)	腺嘌呤核苷酸 VB₈/ (ng∙g⁻¹)	总维生素 ∑VB_i/ (μg∙g⁻¹)
南平五夫 Wufu, Nanping	230	548.51±15.53 a	309.43±6.94 b	266.03±7.83 b	247.52±6.35 b	8.73±0.06 b	344.52±5.54 c	1.82±0.24 a	3.34±1.12 b	10.45±0.049 b
南平横墩 Hengdun, Nanping	380	493.21±6.33 b	320.15±2.05 b	243.42±4.93 c	227.54±6.12 c	7.13±0.03 e	279.14±1.85 e	1.22±0.13 a	2.42±0.44 b	8.70±0.046 e
南平后洋 Houyang, Nanping	419	433.24±4.36 c	349.17±5.42 a	281.22±11.98 a	239.12±6.94 bc	7.68±0.07 d	293.26±2.17 d	1.75±0.87 a	24.05±7.46 a	9.32±0.07 d
南平吴边 Wubian, Nanping	579	498.15±4.56 b	318.74±1.23 b	266.39±3.67 b	521.12±6.74 a	9.38±0.05 a	477.14±2.66 a	1.56±0.35 a	1.14±0.46 b	11.46±0.059 a
南平倪坜 Nili, Nanping	938	411.54±4.33 d	351.92±9.04 a	222.63±3.67 d	236.35±11.33 bc	8.38±0.07 c	393.54±8.67 b	1.64±0.34 a	19.45±1.16 a	10.02±0.07 c
最大值 Max.		566.3	361.63	294.23	528.53	9.44	478.96	2.62	31.08	11.52
最小值 Min.		409.02	302.42	218.76	220.56	7.11	277.04	1.21	0.66	8.67
平均数 Average		476.93	329.91	255.93	294.32	8.26	357.54	1.6	10.01	9.99
变异系数 CV/%		10.74	5.62	8.64	39.99	9.87	20.93	26.04	104.18	9.86

下载: 导出CSV

表 5 同一地区不同海拔花青素、黄酮及类胡萝卜素含量

Table 5. Anthocyanin, flavonoids and carotenoid in grains of rice grown at different elevations in same area

地点 Locations	海拔 Elevation/ m	花青素 Anthocyanin/ (mg∙g⁻¹)	黄酮 Flavonoids/ (mg∙g⁻¹)	类胡萝卜素 Carotenoid/ (μg∙g⁻¹)
南平五夫 Wufu, Nanping	230	0.97±0.01 c	3.82±0.05 d	67.91±0.51 c
南平横墩 Hengdun, Nanping	380	0.97±0.01 c	3.40±0.06 e	51.12±0.42 d
南平后洋 Houyang, Nanping	419	0.99±0.02 c	4.05±0.04 c	44.33±0.23 e
南平吴边 Wubian, Nanping	579	1.77±0.03 a	4.50±0.05 b	95.54±0.92 a
南平倪坜 Nili, Nanping	938	1.50±0.01 b	5.38±0.05 a	77.16±0.41 b
最大值 Max.		1.79	5.43	96.61
最小值 Min.		0.96	3.36	44.22
平均数 Average		1.24	4.23	67.21
变异系数 CV/%		27.83	16.56	28.28

下载: 导出CSV

表 6 相关系数矩阵

Table 6. Correlation coefficient matrix

组分 Component	盐酸硫胺素 VB₁	烟酰胺 VB₃	吡哆醇 VB₆	核黄素 VB₂	腺嘌呤 VB₄	腺嘌呤核苷酸 VB₈	泛酸 VB₅	维生素 H VB₇	花青素 Anthocyanin	黄酮 Flavonoids	类胡萝卜素 Carotenoid
盐酸硫胺素 VB₁	1.00
烟酰胺 VB₃	0.04	1.00
吡哆醇 VB₆	0.11	0.17	1.00
核黄素 VB₂	−0.42	0.21	0.28	1.00
腺嘌呤 VB₄	0.42	−0.03	0.58	0.30	1.00
腺嘌呤核苷酸 VB₈	−0.48	−0.28	−0.30	0.21	−0.36	1.00
泛酸 VB₅	0.43	0.41	0.74	0.25	0.39	−0.45	1.00
维生素 H VB₇	0.46	0.73	0.28	0.16	0.25	−0.23	0.62	1.00
花青素 Anthocyanin	0.20	−0.54	0.52	0.04	0.51	−0.05	0.29	−0.33	1.00
黄酮 Flavonoids	−0.28	−0.20	0.61	0.55	0.21	0.16	0.47	−0.13	0.69	1.00
类胡萝卜素 Carotenoid	0.10	−0.53	0.66	0.05	0.47	−0.20	0.40	−0.32	0.87	0.71	1.00

下载: 导出CSV

表 7 主成分因子荷载矩阵表

Table 7. Load matrix of principal component factor

指标 Index	载荷 Load
指标 Index	主成分1 PC1	主成分2 PC2	主成分3 PC3
盐酸硫胺素 VB₁	0.282	0.512	−0.694
烟酰胺 VB₃	−0.104	0.837	0.407
吡哆醇 VB₆	0.872	0.167	0.175
核黄素 VB₂	0.306	−0.012	0.819
腺嘌呤 VB₄	0.704	0.188	−0.158
腺嘌呤核苷酸 VB₈	−0.337	−0.511	0.455
泛酸 VB₅	0.743	0.537	0.124
维生素 H VB₇	0.148	0.893	0.189
花青素 Anthocyanin	0.788	−0.479	−0.237
黄酮 Flavonoids	0.735	−0.393	0.459
类胡萝卜素 Carotenoid	0.845	−0.438	−0.202

下载: 导出CSV

表 8 总方差分解

Table 8. Partitioning of total variance

成分 Index	总计 Total	提取载荷平方和 Extract the quadratic sum of the loads
成分 Index	总计 Total	方差百分比 Variance proportion/%	累积贡献率 Accumulative contribution rate/%
1	4.002	36.38	36.38
2	2.949	26.81	63.19
3	1.939	17.63	80.82

下载: 导出CSV

表 9 各地点样本主成分得分

Table 9. Scores on principal components for rice from different localities

地点 Location	主成分1 PC1	主成分2 PC2	主成分3 PC3	总得分 Total	种植季节 Planting season	海拔 Elevation/m
泉州瑞坂 Ruiban, Quanzhou	−3.141	−1.717	−0.989	−2.199	中 Middle season	285
南平横墩 Hengdun, Nanping	−2.559	−0.141	−1.353	−1.493	中 Middle season	380
南平后洋 Houyang, Nanping	−2.392	−0.277	1.229	−0.900	中 Middle season	419
南平五夫 Wufu, Nanping	−0.784	1.278	−1.320	−0.216	中 Middle-season	230
莆田南兴 Nanxing, Putian	−0.028	−1.062	1.715	0.008	中 Middle season	590
三明杨城 Yangcheng, Sanming	0.933	−0.461	−0.974	0.054	烟后 Post-tobacco season	380
南平倪坜 Nili, Nanping	0.364	−1.308	1.503	0.057	中 Middle season	938
宁德南湾 Nanwan, Ningde	1.538	−2.013	1.164	0.278	中 Middle season	950
龙岩黄埔 Huangpu, Longyan	1.732	0.635	−1.989	0.556	晚 Late season	242
南平吴边 Wubian, Nanping	2.332	0.235	−1.027	0.903	中 Middle season	579
漳州宝斗 Baodou, Zhangzhou	−0.924	4.480	1.598	1.420	早 Early season	121
宁德岚亭 Lanting, Ningde	2.930	0.351	0.444	1.532	晚 Late season	20

下载: 导出CSV

参考文献(40)

[1]	黄愿偿, 黄河清, 邹学英. 稻米蛋白质含量的遗传研究 [J]. 湖南农业科学, 1985(2):7−9. doi: 10.16498/j.cnki.hnnykx.1985.02.002 HUANG Y C, HUANG H Q, ZOU X Y. Genetic study on protein content in rice [J]. Hunan Agricultural Sciences, 1985(2): 7−9.(in Chinese) doi: 10.16498/j.cnki.hnnykx.1985.02.002
[2]	闵军. 黑米的营养特性 [J]. 湖南农业, 2013(9):41. doi: 10.3969/j.issn.1005-362X.2013.09.056 MIN J. Nutritional characteristics of black rice [J]. Hunan Agricuture, 2013(9): 41.(in Chinese) doi: 10.3969/j.issn.1005-362X.2013.09.056
[3]	孙玲, 张名位, 池建伟, 等. 黑米的抗氧化性及其与黄酮和种皮色素的关系 [J]. 营养学报, 2000, 22(3):246−249. doi: 10.3321/j.issn:0512-7955.2000.03.014 SUN L, ZHANG M W, CHI J W, et al. The antioxidation activity of black rice and its correlation with flavonoids and pigment [J]. Acta Nutrimenta Sinica, 2000, 22(3): 246−249.(in Chinese) doi: 10.3321/j.issn:0512-7955.2000.03.014
[4]	张名位, 郭宝江, 池建伟, 等. 不同品种黑米的抗氧化作用及其与总黄酮和花色苷含量的关系 [J]. 中国农业科学, 2005, 38(7):1324−1331. doi: 10.3321/j.issn:0578-1752.2005.07.007 ZHANG M W, GUO B J, CHI J W, et al. Antioxidations and their correlations with total flavonid and anthocyanin contents in different black rice varieties [J]. Scientia Agricultura Sinica, 2005, 38(7): 1324−1331.(in Chinese) doi: 10.3321/j.issn:0578-1752.2005.07.007
[5]	王梦姝, 吕晓玲, 赵焕焦, 等. 黑米花色苷磷脂复合物的制备及生物利用度 [J]. 食品科技, 2017, 42(5):242−245. doi: 10.13684/j.cnki.spkj.2017.05.047 WANG M S, LYU X L, ZHAO H J, et al. Preparation and bioavailability of black rice anthocyanin phospholipid complex [J]. Food Science and Technology, 2017, 42(5): 242−245.(in Chinese) doi: 10.13684/j.cnki.spkj.2017.05.047
[6]	万明, 杨菠, 付桂明. 黑米资源及其开发利用前景 [J]. 江西食品工业, 2005(2):20−21. doi: 10.3969/j.issn.1674-2435.2005.02.008 WAN M, YANG B, FU G M. Black rice resources and its development and utilization prospect [J]. Jiangxi Food Industry, 2005(2): 20−21.(in Chinese) doi: 10.3969/j.issn.1674-2435.2005.02.008
[7]	吴三桥, 李新生, 闵锁田, 等. 黑米红色素系列产品的开发研究 [J]. 汉中师范学院学报(自然科学), 1997, 15(6):67−69. WU S Q, LI X S, MIN S T, et al. The exploitative research of the senal products of blackrice red pigment [J]. Journal of Hanzhong Teachers College, 1997, 15(6): 67−69.(in Chinese)
[8]	李新生, 吴升华. 陕西黑稻资源及其开发利用 [J]. 资源科学, 1998, 20(6):67−73. LI X S, WU S H. Shaanxi black rice resources and its development and utilization [J]. Resources Science, 1998, 20(6): 67−73.(in Chinese)
[9]	赖来展, 张名位, 彭仲明, 等. 黑米稻种质资源的评价与利用研究 [J]. 作物品种资源, 1994(S1):58−64. LAI L Z, ZHANG M W, PENG Z M, et al. Evaluation and utilization of black rice germplasm resources [J]. Crop Germplasm Resources, 1994(S1): 58−64.(in Chinese)
[10]	赵腾芳. 黑糯稻品种资源的考察 [J]. 云南农业科技, 1985(1):29−31,27. ZHAO T F. Investigation of glutinous rice variety resources [J]. Yunnan Agricultural Science and Technology, 1985(1): 29−31,27.(in Chinese)
[11]	赖来展, 李宝健. 应用生物技术选育黑优粘系列新品种的研究 [J]. 中山大学学报论丛, 1989(4):123−127. LAI L Z, LI B J. The study of the breeding of “black superior rice” by using biotechniques [J]. Sun Yatsen University Forum, 1989(4): 123−127.(in Chinese)
[12]	赵成章, 戚秀芳, 杨长登. 应用无性系变异技术培育黑珍米的研究 [J]. 中国水稻科学, 1993, 7(2):120−122. doi: 10.3321/j.issn:1001-7216.1993.02.012 ZHAO C Z, QI X F, YANG C D. Heizhenmi, A black-kernel rice derived from basmati 370 by somaclonal variation [J]. Chinese Journal of Rice Science, 1993, 7(2): 120−122.(in Chinese) doi: 10.3321/j.issn:1001-7216.1993.02.012
[13]	罗同平, 周忠凤, 莫海玲, 等. 特种黑米新品种桂育黑糯的选育 [J]. 中国种业, 2021(7):80−81. doi: 10.3969/j.issn.1671-895X.2021.07.029 LUO T P, ZHOU Z F, MO H L, et al. Breeding of a new special black rice variety Guiyu Heinuo [J]. China Seed Industry, 2021(7): 80−81.(in Chinese) doi: 10.3969/j.issn.1671-895X.2021.07.029
[14]	任伟, 郜红枫, 于利民, 等. 黑米新品种珍粳168选育及栽培要点 [J]. 黑龙江农业科学, 2020(4):139−141. doi: 10.11942/j.issn1002-2767.2020.04.0139 REN W, GAO H F, YU L M, et al. Key points of cultivation and breeding of a new black rice variety Zhenjing 168 [J]. Heilongjiang Agricultural Sciences, 2020(4): 139−141.(in Chinese) doi: 10.11942/j.issn1002-2767.2020.04.0139
[15]	黄庭旭, 郑建华, 游晴如, 等. 两系特种稻新品种紫两优737的选育与应用 [J]. 福建农业学报, 2020, 35(11):1171−1178. doi: 10.19303/j.issn.1008-0384.2020.11.001 HUANG T X, ZHENG J H, YOU Q R, et al. Breeding and application of new two-line special rice variety Ziliangyou 737 [J]. Fujian Journal of Agricultural Sciences, 2020, 35(11): 1171−1178.(in Chinese) doi: 10.19303/j.issn.1008-0384.2020.11.001
[16]	郑建华, 王洪飞, 周鹏, 等. 籼型紫黑糯稻光温敏核不育系紫392S选育与应用 [J]. 杂交水稻, 2021, 36(6):13−17. doi: 10.16267/j.cnki.1005-3956.20200928.306 ZHENG J H, WANG H F, ZHOU P, et al. Breeding and application of PTGMS line Zi392S of indica-type glutinous rice with purple-black grains [J]. Hybrid Rice, 2021, 36(6): 13−17.(in Chinese) doi: 10.16267/j.cnki.1005-3956.20200928.306
[17]	周鹏, 王洪飞, 陈春霞, 等. 紫糯杂交稻紫两优737在仙游县种植表现及高产栽培技术 [J]. 福建稻麦科技, 2020, 38(4):33−35. doi: 10.3969/j.issn.1008-9799.2020.04.016 ZHOU P, WANG H F, CHEN C X, et al. Demonstration performance of purple waxy hybrid rice Ziliangyou 737 and its high-yielding cultivation techniques at Xianyou County [J]. Fujian Science and Technology of Rice and Wheat, 2020, 38(4): 33−35.(in Chinese) doi: 10.3969/j.issn.1008-9799.2020.04.016
[18]	王洪飞, 周鹏, 涂诗航, 等. 两系杂交紫糯稻紫两优737在宁化种植表现及关键栽培技术 [J]. 福建稻麦科技, 2020, 38(1):34−36. WANG H F, ZHOU P, TU S H, et al. Planting performance and the key cultivation techniques of two-line hybrid purple glutinous rice variety Ziliangyou 737 in Ninghua County [J]. Fujian Science and Technology of Rice and Wheat, 2020, 38(1): 34−36.(in Chinese)
[19]	王洪飞, 陈建云, 郑建华, 等. 两系特种稻紫两优737在云南高原种植表现及丰产栽培技术 [J]. 福建稻麦科技, 2020, 38(2):21−24. doi: 10.3969/j.issn.1008-9799.2020.02.010 WANG H F, CHEN J Y, ZHENG J H, et al. Planting performance and high-yielding cultivation techniques of two-line special rice Ziliangyou 737 in Yunnan Plateau [J]. Fujian Science and Technology of Rice and Wheat, 2020, 38(2): 21−24.(in Chinese) doi: 10.3969/j.issn.1008-9799.2020.02.010
[20]	游晴如, 董练飞, 王洪飞, 等. 两系特种稻紫两优737高产制种技术 [J]. 福建稻麦科技, 2019, 37(3):8−11. doi: 10.3969/j.issn.1008-9799.2019.03.003 YOU Q R, DONG L F, WANG H F, et al. High-yielding seed production technology of two-line special rice Ziliangyou 737 [J]. Fujian Science and Technology of Rice and Wheat, 2019, 37(3): 8−11.(in Chinese) doi: 10.3969/j.issn.1008-9799.2019.03.003
[21]	张圣美, 刘晓慧, 尚静, 等. 高温胁迫对茄子花青素含量及其合成相关酶活性和基因表达的影响 [J]. 上海农业学报, 2020, 36(6):6−12. doi: 10.15955/j.issn1000-3924.2020.06.02 ZHANG S M, LIU X H, SHANG J, et al. Effects of high temperature stress on anthocyanin concentration, enzyme activities related to its synthesis and gene expression in eggplant [J]. Acta Agriculturae Shanghai, 2020, 36(6): 6−12.(in Chinese) doi: 10.15955/j.issn1000-3924.2020.06.02
[22]	唐容, 黄泽素, 代文东, 等. 紫红叶油菜叶片花青素的含量变化及其稳定性 [J]. 西南农业学报, 2017, 30(2):285−290. doi: 10.16213/j.cnki.scjas.2017.2.008 TANG R, HUANG Z S, DAI W D, et al. Variation and stability of anthocyanin content in leaves of rapeseed with purple-red leaves [J]. Southwest China Journal of Agricultural Sciences, 2017, 30(2): 285−290.(in Chinese) doi: 10.16213/j.cnki.scjas.2017.2.008
[23]	郑顺林, 张仪, 李世林, 等. 不同海拔高度对紫色马铃薯产量、品质及花青素含量的影响 [J]. 西南农业学报, 2013, 26(4):1420−1423. doi: 10.3969/j.issn.1001-4829.2013.04.025 ZHENG S L, ZHANG Y, LI S L, et al. Effects of different altitudes on yield, quality and anthocyanin content of purple potato(Solanum tuberosum L. ) [J]. Southwest China Journal of Agricultural Sciences, 2013, 26(4): 1420−1423.(in Chinese) doi: 10.3969/j.issn.1001-4829.2013.04.025
[24]	沈学善, 王平, 吴翠平, 等. 不同光照地区对马铃薯花青素含量及品质的影响 [J]. 西南农业学报, 2017, 30(12):2667−2672. doi: 10.16213/j.cnki.scjas.2017.12.009 SHEN X S, WANG P, WU C P, et al. Effects of light on anthocyanin content and quality of potato in different natural lighting areas [J]. Southwest China Journal of Agricultural Sciences, 2017, 30(12): 2667−2672.(in Chinese) doi: 10.16213/j.cnki.scjas.2017.12.009
[25]	马静, 陈起萱, 凌文华. 红、黑米的保健功效研究 [J]. 食品科学, 2000, 21(12):139−140. doi: 10.3321/j.issn:1002-6630.2000.12.052 MA J, CHEN Q X, LING W H. Study on health care efficacy of red and black rice [J]. Food Science, 2000, 21(12): 139−140.(in Chinese) doi: 10.3321/j.issn:1002-6630.2000.12.052
[26]	周云, 张守文. “特种稻米”功能特性及其开发利用 [J]. 粮食与油脂, 2002, 15(7):36−38. doi: 10.3969/j.issn.1008-9578.2002.07.013 ZHOU Y, ZHANG S W. Functional characteristic and development and utilization of “special rice” [J]. Journal of Cereals & Oils, 2002, 15(7): 36−38.(in Chinese) doi: 10.3969/j.issn.1008-9578.2002.07.013
[27]	单美荣. 维生素B6在脂多糖所诱导的急性心肺损伤中的作用及机制[D]. 济南: 山东大学, 2020. SHAN M R. The role and mechanisms of vitamin B6 in lipopolysaccharide-induced acute cardiopulmonary injury[D]. Jinan: Shandong University, 2020. (in Chinese)
[28]	沙静, 白小岗, 乔向东. 维生素B₁口服与甲钴胺注射联合对糖尿病周围神经病变的疗效分析 [J]. 延安大学学报(医学科学版), 2018, 16(4):43−45. SHA J, BAI X G, QIAO X D. Effectiveness of vitamin B₁ combined with mecobalamin injection for diabetic peripheral neuropathy [J]. Journal of Yanan University (Medical Science Edition), 2018, 16(4): 43−45.(in Chinese)
[29]	邹德堂, 韩笑, 孙健, 等. 有色稻米B族维生素含量与种皮颜色关系研究 [J]. 东北农业大学学报, 2020, 51(9):1−8. doi: 10.3969/j.issn.1005-9369.2020.09.001 ZOU D T, HAN X, SUN J, et al. Study on relationship between B vitamins content and seed coat color of colored rice [J]. Journal of Northeast Agricultural University, 2020, 51(9): 1−8.(in Chinese) doi: 10.3969/j.issn.1005-9369.2020.09.001
[30]	ROMAGNOLO D F, SELMIN O I. Flavonoids and cancer prevention: A review of the evidence [J]. Journal of Nutrition in Gerontology and Geriatrics, 2012, 31(3): 206−238. doi: 10.1080/21551197.2012.702534
[31]	周丹蓉, 方智振, 叶新福, 等. 李果中花色素苷研究进展 [J]. 东南园艺, 2015, 3(3):43−46. doi: 10.3969/j.issn.1004-6089.2015.03.012 ZHOU D R, FANG Z Z, YE X F, et al. Research progress of anthocyanin in plum [J]. Southeast Horticulture, 2015, 3(3): 43−46.(in Chinese) doi: 10.3969/j.issn.1004-6089.2015.03.012
[32]	陈继卫, 沈朝栋, 贾玉芳, 等. 鸡爪槭转色期叶色变化生理研究 [J]. 核农学报, 2010, 24(1):171−175,180. CHEN J W, SHEN C D, JIA Y F, et al. Physiological changes of Japanese maple(Acer palmatum thunb)leaves during color-changing period [J]. Journal of Nuclear Agricultural Sciences, 2010, 24(1): 171−175,180.(in Chinese)
[33]	孙苏南. 三杉秋冬叶色变化及植物造景研究[D]. 杭州: 浙江农林大学, 2013. SUN S N. Color changes on leaves and landscape design of Metasequoia glyptostroboides, Taxodium ascendens, Taxodium distichum[D]. Hangzhou: Zhejiang A & F University, 2013. (in Chinese)
[34]	李小康. 中红杨叶色变化的生理生化研究[D]. 郑州: 河南农业大学, 2008. LI X K. Study on changes of physiology and biochemistry during its leafcolour transformation of Populus × euramericana’Zhonghong’[D]. Zhengzhou: Henan Agricultural University, 2008. (in Chinese)
[35]	费芳, 王慧颖, 唐前瑞. 温度对红花檵木叶色的影响研究 [J]. 湖南理工学院学报(自然科学版), 2008, 21(2):88−90. FEI F, WANG H Y, TANG Q R. Study on the impact of temperature on Loropetalum Chinese leaves color [J]. Journal of Hunan Institute of Science and Technology (Natural Sciences), 2008, 21(2): 88−90.(in Chinese)
[36]	庞学群, 张昭其, 段学武, 等. pH值和温度对荔枝果皮花色素苷稳定性的影响 [J]. 园艺学报, 2001, 28(1):25−30. PANG X Q, ZHANG S Q, DUAN X W, et al. Influence of pH and temperature on the stability of anthocyanin from Litchi pericarp [J]. Acta Horticulturae Sinica, 2001, 28(1): 25−30.(in Chinese)
[37]	韦忠刚. 遮荫对元宝枫花色素苷含量的影响 [J]. 天津农业科学, 2017, 23(3):6−8,16. doi: 10.3969/j.issn.1006-6500.2017.03.002 WEI Z G. Effects of shading on the content of anthocyanin in Acer truncatum [J]. Tianjin Agricultural Sciences, 2017, 23(3): 6−8,16.(in Chinese) doi: 10.3969/j.issn.1006-6500.2017.03.002
[38]	BELL C. Secondary plant products [J]. Encyclopedia of Plant Physiology, 1980, 8(1): 340−349.
[39]	REINERT J, YEOMAN M M. Plant Cell and Tissue Culture[M]. Berlin: Springer Verlag, 1982, 4 (6): 48-49.
[40]	杨加珍, 曾亚文, 杜娟, 等. 紫黑米种质功能成分综合研究与利用 [J]. 生物技术进展, 2015, 5(1):47−53. doi: 10.3969/j.issn.2095-2341.2015.01.07 YANG J Z, ZENG Y W, DU J, et al. Comprehensive utilization and research of functional components of purple black rice germplasm [J]. Current Biotechnology, 2015, 5(1): 47−53.(in Chinese) doi: 10.3969/j.issn.2095-2341.2015.01.07