添加猴樟基质对牛樟芝生长及生理生化特性的影响

黄兴连; 宝元朵; 杨志娟; 陈小涛; 郑元

doi:10.19303/j.issn.1008-0384.2022.012.010

添加猴樟基质对牛樟芝生长及生理生化特性的影响

doi: 10.19303/j.issn.1008-0384.2022.012.010

黄兴连^{1, 2,},
宝元朵^{1, 2},
杨志娟^{1, 2},
陈小涛^{1, 2},
郑元^1, ,

1.
西南林业大学林学院，云南　昆明　650224
2.
云南省森林灾害预警与控制重点实验室，云南　昆明　650224

基金项目: 国家自然科学基金项目（32160736）；云南省基础研究计划面上项目（202101AT070044）；云南省重大科技专项计划食用菌项目子课题（202002AE320003）

详细信息

作者简介:
黄兴连（1999−），男，研究方向：植物生理学和药食用菌学（E-mail：2870012173@qq.com）

通讯作者:
郑元（1982−），男，副教授，研究方向：植物生理学和药食用菌学（E-mail：zhengyuan_001@126.com）

中图分类号: S 567.3
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- 被引次数: 0
出版历程
- 收稿日期: 2022-07-24
- 修回日期: 2022-09-09
- 网络出版日期: 2022-12-28
- 刊出日期: 2022-03-28

Effects of Cinnamomum bodinieri Addition in Culture Substrate on Growth and Physiochemical Characteristics of Antrodia cinnamomea

HUANG Xinglian^{1, 2
,},
BAO Yuanduo^{1, 2},
YANG Zhijuan^{1, 2},
CHEN Xiaotao^{1, 2},
ZHENG Yuan^{1
, ,}

1.
Forestry College, Southwest Forestry University, Kunming, Yunnan　650224, China
2.
Southwest Forestry University/Yunnan Provincial Key Laboratory of Forest Disaster Early Warning and Control, Kunming, Yunnan　650224, China

摘要

摘要: 目的明确添加猴樟Cinnamomum bodinieri基质对牛樟芝Antrodia cinnamomea生长及生理生化特性的影响，并筛选出适宜的猴樟茎、叶浓度。方法比较分析在牛樟芝基础培养基中添加质量浓度分别为0.125 、0.25、0.5、1 、2 、4、8 、16、32 、64 g·L⁻¹的猴樟嫩枝、嫩叶、枝叶混合物时，牛樟芝的生长特性、生物量、SOD活性和总三萜（TT）含量的差异。结果在PDA培养基中添加质量浓度分别为0.5～4 g·L⁻¹、2～8 g·L⁻¹、1～4 g·L⁻¹、4～16 g·L⁻¹的猴樟嫩枝基质，牛樟芝相应表现出生长特性较优、生物量、SOD活性和TT含量较高的优势，并显著高于对照水平；在PDA培养基中添加猴樟嫩叶基质质量浓度为1～2 g·L⁻¹时，其生长特性较优，SOD活性较高，当添加量为2～4 g·L⁻¹时，其生物量和TT含量较高；在PDA培养基中添加猴樟枝叶混合物基质质量浓度分别为2～4 g·L⁻¹、1～4 g·L⁻¹、0.125～1 g·L⁻¹、4～8 g·L⁻¹时，其相应表现为生长特性较优、生物量、SOD活性和TT含量较高的优势。结论研究结果表明，总体来说，在PDA培养基中添加猴樟基质均能促进牛樟芝菌丝体、生物量、SOD活性和总三萜含量的提高，其中猴樟嫩叶基质2 g·L⁻¹对菌丝体和生物量的提高最显著、猴樟嫩枝1 g·L⁻¹或嫩叶基质2 g·L⁻¹对菌丝体SOD活性的促进效果最显著；猴樟嫩枝基质8 g·L⁻¹，对菌丝体总三萜含量的促进效果最显著，达到23.73 mg·g⁻¹，较对照组提高了81.77%。该结果是樟属植物对牛樟芝培养的补充，为牛樟芝的规模化生产和开发利用提供了理论依据。
- 牛樟芝 /
- 猴樟 /
- 皿式培养 /
- 生长特性 /
- 总三萜 /
- 超氧化物歧化酶
Abstract: Objective Effects and optimal amounts of branches and/or leaves of Cinnamomum bodinieri added in culture substrate on the growth and physiochemical characteristics of Antrodia cinnamomea were determined. Method The growth characteristics, biomass, SOD activity and total triterpenes (TT) content of A. cinnamomea were compared and analyzed when the young branches, leaves, both branches and leaves of C. bodinieri was added with mass concentration of 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64 g·L⁻¹ in PDA medium. Result When various concentrations of C. bodinieri young branches 0.5–4 g·L⁻¹, 2–8 g·L⁻¹, 1–4 g·L⁻¹and 4–16 g·L⁻¹ were added to the PDA medium, the growth, biomass, SOD activity, and TT content of A. cinnamomea were significantly higher than those of control without the addition. With 1–2 g·L⁻¹ added leaves in the substrate, A. cinnamomea grew well with an increased SOD activity; while at 2–4 g·L⁻¹, raised biomass and TT content. By adding both branches and leaves at 2–4 g·L⁻¹, 1–4 g·L⁻¹, 0.125–1 g·L⁻¹ and 4–8 g·L⁻¹, the mushroom growth could be improved with increases on biomass, SOD activity, and TT content. Conclusion The results showed that, in general, the mycelium, biomass, SOD activity and total triterpene content of A.cinnammomea were increased by adding C. bodinieri in PDA mediu. Among them, 2 g·L⁻¹ C. bodinieri young leaves significantly increased mycelium and biomassm. C.bodinieri young branches 1 g·L⁻¹or leaves 2 g·L⁻¹, the promotion effect of SOD activity in mycelium was the most significant. The 8 g·L⁻¹ branches of C. bodinieri had the most significant promoting effect on the total triterpenoid content of mycelium, reaching 23.73 mg·g⁻¹, which was increased by 81.77% compared with the control group. This result is a supplement to the culture of A. cinnammomea , and provided a theoretical basis for the large-scale production, development and utilization of A. cinnammomea.
- Antrodia cinnamomea /
- Cinnamomum bodinieri /
- dish culture /
- growth characteristics /
- total triterpenes /
- superoxide dismutase

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图 1 牛樟芝菌丝体生长表型

Z：猴樟嫩枝；Y：猴樟嫩叶；ZY：猴樟枝叶混合。

Figure 1. Growth phenotype of A.cinnamomea

Z: C. bodinieri branches; Y: C. bodinieri leaves; ZY: C. bodinieri both branches and leaves.

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图 2 添加猴樟基质对牛樟芝SOD活性的影响

A：猴樟嫩枝基质；B：猴樟嫩叶基质；C：猴樟枝叶混合基质。小写字母表示在0.05水平差异显著，下同。

Figure 2. Effect of C. bodinieri addition in culture substrate on SOD activity of A. cinnamomea

A: C. bodinieri branches; B: C. bodinieri leaves; C: both branches and leaves of C. bodinieri. Data with different lowercase letters indicate significant differences at 0.05 level. Same for Fig.3.

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图 3 添加猴樟基质对牛樟芝总三萜含量(TT)的影响

Figure 3. Effect of C. bodinieri addition in culture substrate on TT content of A. cinnamomea

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表 1 添加猴樟嫩枝基质对牛樟芝生长特性的影响

Table 1. Effect of C. bodinieri branches addition in culture substrate on growth of A. cinnamomea

处理 Treatments	菌落直径 Colony diameter/mm	菌落颜色 Colony color	菌丝密度 Colony density	菌落长势 Growth tendency	生长速度 Growth speed/(mm·d⁻¹)	生长指数 Growth index
PDA-0	34.08±9.82 f	☆	++	1.5	1.14±0.33 e	1.71±0.50 f
Z-0.125	47.20±6.50 d	☆	++	1.5	1.58±0.22 d	2.37±0.33 e
Z-0.25	51.93±8.20 cd	☆☆	+++	2.5	1.73±0.27 cd	4.33±0.68 d
Z-0.5	58.60±8.56 ab	☆☆☆	+++	3	1.95±0.29 bc	5.85±0.87 ab
Z-1	59.88±10.58 ab	☆☆☆	+++	3	1.90±0.35 bc	5.70±1.05 ab
Z-2	62.36±10.33 a	☆☆☆	+++	3	2.00±0.34 ab	6.24±1.02 a
Z-4	55.30±7.69 bc	☆☆	+++	2.5	1.84±0.26 bc	4.60±0.65 bc
Z-8	51.92±7.00 cd	☆☆	+++	2.5	1.73±0.23 cd	4.33±0.58 d
Z-16	49.71±9.65 d	☆☆	+++	2.5	1.99±0.32 b	4.98±0.8 b
Z-32	39.93±5.23 e	☆☆	++	2	2.00±0.17 b	4.00±0.34 d
Z-64	38.50±5.16 ef	☆☆	++	2	2.28±0.17 a	4.56±0.34 cd
① “+”越多，密度越高；“☆”越多，颜色越深；菌落长势评级数值越高，长势越好。菌落长势的评级标准= (菌丝密度×菌落颜色)/2^[22] 。② 表中不同小写字母表示在0.05水平差异显著；③ Z：猴樟嫩枝；Y：猴樟嫩叶；ZY：猴樟嫩枝叶混合；基质代号后的数值为添加量 (g·L⁻¹)。下同。 ① More "+" indicates greater density; more "☆", darker color; and higher colony growth rating, better colony growth. Rating standard of colony growth = (mycelium density × colony color) /2^[22].② Data with different lowercase letters on same column indicate significant differences at 0.05 level. ③ Z: C. bodinieri branches; Y: C. bodinieri leaves; ZY: C. bodinieri both branches and leaves. Numbers in the treatment name were addition amount of C.bodinieri branches(g·L^-1). Same for below.

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表 2 添加猴樟嫩叶基质对牛樟芝生长特性的影响

Table 2. Effect of C. bodinieri leaves addition in culture substrate on growth of A. cinnamomea

处理 Treatments	菌落直径 Colony diameter/mm	菌落颜色 Colony color	菌丝密度 Colony density	菌落长势 Growth tendency	菌落生长速度 Growth speed/(mm·d⁻¹)	生长指数 Growth Index
PDA-0	34.08±9.82 e	☆	++	1.5	1.14±0.33 b	1.71±0.59 g
Y-0.125	53.75±0.75 cd	☆☆	++	2	1.79±0.02 ab	3.58±0.04 f
Y-0.25	54.17±6.18 cd	☆☆	++	2	2.14±0.54 ab	4.28±1.08 de
Y-0.5	58.70±9.08 c	☆☆	++	2	1.96±0.30 ab	3.92±0.60 ef
Y-1	71.19±6.96 b	☆☆☆	+++++	4	2.37±0.23 a	9.49±0.92 a
Y-2	86.60±4.28 a	☆☆☆	++++	3.5	2.65±0.14 a	9.28±0.49 a
Y-4	69.44±8.49 b	☆☆	+++	2.5	2.11±0.28 ab	5.28±0.70c
Y-8	56.50±6.35 c	☆☆	+++	2.5	2.55±0.21 a	6.38±0.53 b
Y-16	52.25±7.49 cd	☆☆	+++	2.5	2.41±0.25 ab	6.03±0.63 b
Y-32	42.42±8.82 de	☆☆	++	2	2.41±0.29 ab	4.82±0.48 cd
Y-64	35.50±8.89 e	☆☆	++	2	2.52±0.30 ab	5.04±0.60 c

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表 3 添加猴樟枝叶混合物基质对牛樟芝生长特性的影响

Table 3. Effect of adding mixture of C. bodinieri branches and leaves in culture substrate on growth of A. cinnamomea

处理 Treatments	菌落直径 Colony diameter/mm	菌落颜色 Colony color	菌丝密度 Colony density	菌落长势 Growth tendency	菌落生长速度 Growth speed/(mm·d⁻¹)	生长指数 Growth index
PDA-0	34.08±4.82 d	☆	++	1.5	1.14±0.33 c	1.71±0.50 e
ZY-0.125	47.86±5.67 abcd	☆☆	++	2	1.93±0.36 ab	4.83±0.90 bc
ZY-0.25	52.42±6.30 abc	☆☆	++	2	1.75±0.21 ab	4.37±0.53 cd
ZY-0.5	55.57±1.95 abc	☆☆	+++	2.5	1.69±0.07 b	5.06±0.21 b
ZY-1	57.21±3.75 ab	☆☆☆	++	2.5	1.91±0.12 ab	4.78±0.30 bcd
ZY-2	61.06±7.80 a	☆☆☆☆☆	++++	4.5	2.04±0.26 ab	6.12±0.78 a
ZY-4	61.94±5.10 a	☆☆☆☆	++++	4	2.06±0.17 a	6.19±0.51 a
ZY-8	54.63±8.84 abc	☆☆☆	+++	3	1.82±0.29 ab	4.55±0.73 cd
ZY-16	48.17±7.94 abcd	☆☆	+++	2.5	1.94±0.29 ab	4.85±073 bc
ZY-32	43.10±5.62 bcd	☆☆	++	2	2.44±0.19 ab	4.88±0.38 bc
ZY-64	42.25±10.08 cd	☆☆	++	2	2.08±0.34 a	4.16±0.68 d

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表 4 添加猴樟基质对牛樟芝生物量的影响

Table 4. Effect of C. bodinieri addition in culture substrate on biomass of A. cinnamomea

处理 Treatments	干重 Dry weight/g	处理 Treatments	干重 Dry weight/g	处理 Treatments	干重 Dry weight/g
PDA-0	0.06±0.01 d	PDA-0	0.06±0.01 d	PDA-0	0.06±0.01 e
Z-0.125	0.07±0.02 cd	Y-0.125	0.07±0.02 d	ZY-0.125	0.09±0.01 de
Z-0.25	0.09±0.01 bcd	Y-0.25	0.07±0.03 d	ZY-0.25	0.11±0.02 bcde
Z-0.5	0.11±0.02 bcd	Y-0.5	0.09±0.03 cd	ZY-0.5	0.16±0.05 abcd
Z-1	0.12±0.03 bcd	Y-1	0.14±0.02 bcd	ZY-1	0.20±0.02 a
Z-2	0.19±0.03 a	Y-2	0.24±0.07 a	ZY-2	0.19±0.05 ab
Z-4	0.15±0.06 ab	Y-4	0.20±0.05 ab	ZY-4	0.17±0.04 abc
Z-8	0.13±0.03 abc	Y-8	0.18±0.04 abc	ZY-8	0.16±0.04 abcd
Z-16	0.12±0.02 bcd	Y-16	0.13±0.06 bcd	ZY-16	0.16±0.04 abcd
Z-32	0.09±0.02 bcd	Y-32	0.11±0.05 bcd	ZY-32	0.14±0.03 abcd
Z-64	0.09±0.02 bcd	Y-64	0.10±0.02 bcd	ZY-64	0.10±0.02 cde

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