3种红树植物根际与非根际土壤细菌群落结构及多样性特征

曾志浩; 袁宗胜; 陈雪莹; 林鸿艳; 黄晓南; 刘芳

doi:10.19303/j.issn.1008-0384.2022.06.017

3种红树植物根际与非根际土壤细菌群落结构及多样性特征

doi: 10.19303/j.issn.1008-0384.2022.06.017

1.
福建农林大学生命科学学院，福建　福州　350002
2.
闽江学院地理与海洋学院，福建　福州　350108
3.
福州市海洋与渔业技术中心，福建　福州　350007

基金项目: 福建省林业科技项目（2021FKJ07）；国家自然科学基金项目（32002108）；福建省教育厅青年项目（JAT170201）

详细信息

作者简介:
曾志浩（2001−），男，研究方向：微生物资源开发与利用（E-mail：515468043@qq.com）

通讯作者:
刘芳（1980−），女，博士，讲师，研究方向：微生物资源开发与利用（E-mail：fjliufang@163.com）

中图分类号: Q 938
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- 被引次数: 0
出版历程
- 收稿日期: 2022-02-13
- 修回日期: 2022-04-24
- 网络出版日期: 2022-08-07
- 刊出日期: 2022-06-28

Microbial Community Structure and Diversity in Rhizosphere and Non-rhizosphere Soils at Fields of Three Varieties of Mangrove Plants

1.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
College of Geography and Oceanography, Minjiang University, Fuzhou, Fujian　350108, China
3.
Fuzhou Ocean and Fisheries Technology Center, Fuzhou, Fujian　350007, China

摘要

摘要: 目的研究秋茄、白骨壤、桐花树等3种红树植物根际与非根际土壤细菌群落结构及多样性特征，为阐明土壤微生物群落对红树植物的生长及其在红树林生态系统保护与修复中的重要作用提供科学支撑。方法提取3种红树植物根际土壤及同一区域非根际土壤宏基因组DNA，利用Illumina NovaSeq测序技术，分析土壤细菌群落结构及多样性。结果 ①4组土壤样本共鉴定出细菌39门、55纲、126目、244科、511属，非根际土壤样本OTU数目大于3组红树植物土壤样本，同时其在观测物种数和多样性指数上也表现出了更高的丰富性与多样性；②4组土壤样本中变形菌门的相对丰度均大于60%，占据绝对主导地位。4组土壤样本之间相比较，秋茄根际土壤样本中厚壁菌门和梭杆菌门有更高的丰度，桐花树根际土壤样本里变形菌门的丰度有一定优势，白骨壤根际土壤样本里拟杆菌门的丰度高于其他3组。非根际土壤样本里的芽单胞菌门略微占有优势，但是其在拟杆菌门的丰度却低于3组根际土壤样本；③PCA分析发现桐花树与白骨壤根际土壤的群落组成较为接近，4组样本的根际细菌群落对应的 COG 类目大致相同。结论红树植物非根际土壤的细菌群落有更高的多样性，不同红树植物的重要作用类群不同。
- 红树植物 /
- 细菌 /
- 群落结构 /
- 多样性
Abstract: Objective Structure and diversity of the microbial communities in the rhizosphere and non-rhizosphere soils on lots grown with different mangrove plants were studied for improved protection and restoration of the ecosystem. Methods Metagenomic DNAs were extracted from the rhizosphere and field soils on Kandelia obovata, Avicennia marina, Aegiceras corniculatum lots and the field soil at an open lot for Illumina NovaSeq sequencing to determine the microbial community structure and diversity. Results ① A total of 39 phyla, 55 classes, 126 orders, 244 families, and 511 genera of microbes were identified in the soils. There were more OTUs in the non-rhizosphere field soils, as well as higher number of species and diversity index, than in the rhizosphere soils. ② In the 4 field soils, the greater than 60% relative abundances of Proteobacteria were significantly dominant in the communities. Firmicutes and Fusobacterium were more abundant on the lot at K. obovate field; Proteobacteria at A. corniculatum field; and Bacteroidetes at A. marina field. At phylum level, Bacillus was slightly more in the field soils, but Bacteroidetes less, than in their rhizosphere counterparts. ③ The PCA analysis indicated the microbial compositions of the rhizosphere soils at the A. corniculatum and A. marina fields to be similar, and the corresponding COG categories of communities in rhizosphere soils roughly the same. Conclusion The microbial communities in the non-rhizosphere field soils were more diverse than in the rhizosphere soils at the lots of various mangrove plants. The major functional microbes in the communities differed by the mangrove variety that grew on the soil.
- Mangrove plants /
- microbes /
- community structure /
- diversity

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图 1 相似度为97%水平下的稀释曲线

Figure 1. Dilution curve at 97% similarity level

下载: 全尺寸图片幻灯片

图 2 土壤样本细菌OTU数量与分布

a，4组土壤样本比较；b， 3组根际土壤样本比较。

Figure 2. Number and distribution of microbial OTUs in soils

a: comparison of 4 non-rhizosphere field soil samples; b: comparison of 3 rhizosphere soil samples.

下载: 全尺寸图片幻灯片

图 3 4组样本细菌群落在门水平上的相对丰度

Figure 3. Relative microbial abundance at phylum level in communities of 4 sets of soils

下载: 全尺寸图片幻灯片

图 4 4组样本细菌群落在纲水平的相对丰度

Figure 4. Relative microbial abundance at class level in communities of 4 sets of soils

下载: 全尺寸图片幻灯片

图 5 4组样本细菌群落在目水平的相对丰度

Figure 5. Relative microbial abundance at order level in communities of 4 sets of soils

下载: 全尺寸图片幻灯片

图 6 4组样本细菌群落在科水平的相对丰度

Figure 6. Relative microbial abundance at family level in communities of 4 sets of soils

下载: 全尺寸图片幻灯片

图 7 4组细菌群落在属水平的相对丰度

Figure 7. Relative microbial abundance at genus level in communities of 4 sets of soils

下载: 全尺寸图片幻灯片

图 8 土壤细菌PCA分析

Figure 8. PCA analysis on bacteria in soils

下载: 全尺寸图片幻灯片

图 9 4组样本土壤细菌功能预测

Figure 9. Functional prediction of microbes in 4 sets of soils

下载: 全尺寸图片幻灯片

图 10 COG数目韦恩图

Figure 10. Venn diagram of COG numbers

下载: 全尺寸图片幻灯片

表 1 4组土壤样本的Alpha 多样性指数

Table 1. Alpha diversity indices of 4 sets of soils

样本 Sample	观测物种数 Observed species	丰度指数 ACE	谱系多样性指数 PD whole tree	香农指数 Shannon	文库覆盖率 Coverage/ %
QQ.D	2642	3479.534	269.80	9.47	95.8
QB.D	2689	3478.222	268.81	9.46	95.9
QT.D	2412	3019.951	247.34	9.17	96.6
QCK.D	2770	3696.540	271.26	9.67	95.6

下载: 导出CSV

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