基于流式细胞术对朱砂根种质资源基因组大小的测定

康阳; 赵凯; 刘钰颖; 王钦; 涂松; 王睿昕; 朱云君; 刘梓富; 彭东辉

doi:10.19303/j.issn.1008-0384.2023.02.002

基于流式细胞术对朱砂根种质资源基因组大小的测定

doi: 10.19303/j.issn.1008-0384.2023.02.002

康阳^{1, 2,},
赵凯³,
刘钰颖^{1, 2},
王钦^{1, 2},
涂松^{1, 2},
王睿昕^{1, 2},
朱云君^{1, 2},
刘梓富⁴,
彭东辉^{1, 2, ,}

1.
福建农林大学风景园林学院，福建　福州　350002
2.
福建省观赏植物种质资源创新与应用工程技术研究中心，福建　福州　350002
3.
福建师范大学生命科学学院，福建　福州　350100
4.
武平县林业局，福建　武平　364300

基金项目: 福建省科技计划项目（2022L3008）；福建省林业科技推广项目(2021TG02)

详细信息

作者简介:
康阳（1997−），男，硕士研究生，研究方向：园林植物与应用（E-mail：1459666833@qq.com）

通讯作者:
彭东辉（1971-），男，博士，教授，研究方向：园林植物与应用（E-mail：fjpdh@126.com）

中图分类号: S686
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- 文章访问数: 310
- HTML全文浏览量: 153
- PDF下载量: 25
- 被引次数: 0
出版历程
- 收稿日期: 2022-08-25
- 修回日期: 2022-10-08
- 网络出版日期: 2023-03-05
- 刊出日期: 2023-02-28

Flow Cytometric Determination on Genome Size of Ardisia crenata Germplasms

KANG Yang^{1, 2
,},
ZHAO Kai³,
LIU Yuying^{1, 2},
WANG Qin^{1, 2},
TU Song^{1, 2},
WANG Ruixin^{1, 2},
ZHU Yunjun^{1, 2},
LIU Zifu⁴,
PENG Donghui^{1, 2
, ,}

1.
College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Fujian Engineering Research Center of Ornamental Plant Germplasm Resources Innovation and Application, Fuzhou, Fujian　350002, China
3.
Fujian Normal University, Fuzhou, Fujian　350100, China
4.
Wuping County Forestry Bureau, Fujian　364300, China

摘要

摘要: 目的基于流式细胞术初步探索测定朱砂根基因组大小的方法和流程，为朱砂根基因组文库的建立、基因组全序列测定及其基因组学研究等工作的开展提供基础数据。方法以24份朱砂根（Ardisia crenata）种质资源为供试材料，包括22个人工选育品种和2个野生种质资源，并以番茄（Lycopersicon esculentum）作为内参样本，利用流式细胞术对朱砂根基因组大小进行测定。结果 24份朱砂根基因组大小（C值）为1.77～2.41 Gb，平均大小1.87 Gb；其中玛瑙红（Z-17）、霞珠（Z-20）、珠塔（Z-22） C值最小，均为1.77 Gb，赤丹 C最大，为2.41Gb，部分品种间基因组大小存在一定程度的差异。结论首次测定朱砂根种质资源的基因组大小，其研究结果可为朱砂根基因组文库的建立、基因组全序列测定及其基因组学研究等工作的开展提供基础数据。
- 朱砂根 /
- 流式细胞术 /
- 基因组大小 /
- 种质资源
Abstract: Objective Genome sizes of the economically, medicinally, and ornamentally valuable Ardisia crenata were determined using flow cytometry. Methods Genome sizes of 22 selectively bred cultivars and two wild A. crenata germplasms, along with Lycopersicon esculentum as internal reference, were determined by flow cytometry. Results The sizes ranged from 1.77 Gb to 2.41 Gb averaging 1.87 Gb with the smallest C-value of 1.77 Gb found on Agate Red (Z-17), Kasumi Pearl (Z-20), and Zhu Ta (Z-22), while the largest of 2.41 Gb on Chidan. Variations existed among the germplasms. Conclusion For the first time, the genome sizes of various A. crenata were determined to be available for library construction and genomics studies on the valuable natural resource.
- Ardisia crenata /
- flow cytometry /
- genome size /
- germplasms

HTML全文

图 1 流式细胞仪检测24份朱砂根荧光峰值

荧光峰值直方图左侧峰代表内参番茄，右侧峰代表朱砂根种质；FL2-H:荧光脉冲高度。

Figure 1. Fluorescence peak histogram of 24 A. crenata detected by flow cytometry

Left peak on fluorescence histogram represents internal reference L. esculentum; right peak, A. crenata varieties.FL2-H:Fluore scence pulse height.

下载: 全尺寸图片幻灯片

表 1 24份朱砂根供试材料采样信息

Table 1. Sampling of 24 A. crenata germplasms

编号 No.	种质名称 Germplasm name	种质类别 Germplasm type	编号 No.	种质名称 Germplasm name	种质类别 Germplasm type
Z-01	赤丹 Chi Dan	栽培品种 Cultivated variety	Z-13	福株 Fu Zhu	栽培品种 Cultivated variety
Z-02	锦绣 Jin Xiu	栽培品种 Cultivated variety	Z-14	金边富贵 Jin Bian Fu Gui	栽培品种 Cultivated variety
Z-03	绿翡翠 Green Jade	栽培品种 Cultivated variety	Z-15	金玉满堂 Jin Yu Man Tang	栽培品种 Cultivated variety
Z-04	粉佳人 Pink Beauty	栽培品种 Cultivated variety	Z-16	竹叶富贵 Bamboo Leaf Fu Gui	栽培品种 Cultivated variety
Z-05	平安富贵 Ping An Fu Gui	栽培品种 Cultivated variety	Z-17	玛瑙红 Onyx Red	栽培品种 Cultivated variety
Z-06	金冠 Golden Crown	栽培品种 Cultivated variety	Z-18	碧珠 Bi Zhu	栽培品种 Cultivated variety
Z-07	福满堂 Fu Man Tang	栽培品种 Cultivated variety	Z-19	龙珠 Long Zhu	栽培品种 Cultivated variety
Z-08	碧霞珠 Bixia Zhu	栽培品种 Cultivated variety	Z-20	霞珠 Xia Zhu	栽培品种 Cultivated variety
Z-09	金富贵 Jin Fu Gui	栽培品种 Cultivated variety	Z-21	仙桃 Xian Tao	栽培品种 Cultivated variety
Z-10	梁野富贵 Liang Ye Fu Gui	栽培品种 Cultivated variety	Z-22	珠塔 Zhu Ta	栽培品种 Cultivated variety
Z-11	赤玲珑 Red Lingerie	栽培品种 Cultivated variety	Z-(A)	朱砂根A A. crenata A	野生种质 Wild germplasm
Z-12	大富贵 Da Fu Gui	栽培品种 Cultivated variety	Z-(B)	朱砂根B A. crenata B	野生种质 Wild germplasm

下载: 导出CSV

表 2 流式细胞术测定的24份朱砂根品种资源基因组大小

Table 2. Genome sizes of 24 A. crenata determined by flow cytometry

样品编号Sample No.	内参荧光强度Internal reference fluorescence intensity	待测样品荧光强度Fluorescence intensity of the sample to be measured	比值Ratio	基因组大小Genome/Gb	样品编号Sample No.	内参荧光强度Internal reference fluorescence intensity	待测样品荧光强度Fluorescence intensity of the sample to be measured	比值Ratio	基因组大小Genome/Gb
Z-01	18.90	51.79	2.74	2.41	Z-13	26.61	63.08	2.37	2.09
Z-02	23.83	48.40	2.03	1.79	Z-14	27.09	65.13	2.40	2.12
Z-03	24.33	49.58	2.04	1.79	Z-15	27.22	64.87	2.38	2.10
Z-04	26.06	55.25	2.12	1.87	Z-16	26.68	55.70	2.09	1.84
Z-05	18.75	48.60	2.59	2.28	Z-17	26.63	53.53	2.01	1.77
Z-06	21.30	52.18	2.45	2.16	Z-18	27.19	55.46	2.04	1.79
Z-07	22.72	56.47	2.49	2.19	Z-19	27.61	55.73	2.02	1.78
Z-08	22.44	55.92	2.49	2.19	Z-20	28.26	56.70	2.01	1.77
Z-09	24.16	51.55	2.13	1.88	Z-21	28.56	58.22	2.04	1.79
Z-10	26.36	55.40	2.10	1.85	Z-22	28.34	56.89	2.01	1.77
Z-11	26.07	59.64	2.29	2.01	Z-(A)	28.77	58.62	2.04	1.79
Z-12	25.35	51.83	2.04	1.80	Z-(B)	28.54	58.29	2.04	1.80

下载: 导出CSV

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