番茄<i>GT</i>-1基因组织表达模式及非生物胁迫、植物生长调节剂响应分析

崔宝禄; 陈忠平; 孙婷婷; 龙俊霖; 王先霞; 曾思进; 石怡然

doi:10.19303/j.issn.1008-0384.2023.02.003

番茄GT-1基因组织表达模式及非生物胁迫、植物生长调节剂响应分析

doi: 10.19303/j.issn.1008-0384.2023.02.003

1.
九江学院，江西　九江　332000
2.
黔南民族师范学院，贵州　都匀　558000

基金项目: 国家自然科学基金项目（31960605）

详细信息

作者简介:
崔宝禄（1979−），男，博士，教授，研究方向：植物分子生物学（E-mail：cuibaolu98@163.com）

中图分类号: S641.2
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- 文章访问数: 310
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- 被引次数: 0
出版历程
- 收稿日期: 2022-02-17
- 录用日期: 2022-02-17
- 修回日期: 2022-11-04
- 网络出版日期: 2023-04-14
- 刊出日期: 2023-02-28

Expressions and Responses to Abiotic Stresses and Plant Growth Regulator of Tomato GT-1

1.
Jiujiang University, Jiujiang, Jiangxi　332000, China
2.
Qiannan Normal University for Nationalities, Duyun, Guizhou　558000, China

摘要

摘要: 目的完善番茄GT-1亚家族基因的相关功能信息，为进一步研究Trihelix转录因子调控植物生长发育过程、提高植物非生物胁迫的抗性能力提供参考。方法利用生物信息学方法对GT-1基因进行生物进化分析，利用RT-PCR技术鉴定GT-1基因对非生物胁迫和植物生长调节剂的响应。结果（1）番茄中包含3个GT-1基因，即SlGT-21、SlGT-24和SlGT-35，进化分析表明番茄GT-1基因存在功能分化。（2）表达模式分析发现，3个基因于番茄所有组织中均表达，特别是果实发育阶段，推测SlGT-21、SlGT-35有部分类似功能。（3）3个GT-1基因受干旱抑制，但SlGT-24受抑制更明显；3个基因均响应盐胁迫，SlGT-21、SlGT-35基因被较明显抑制。（4）3个基因受植物生长调节剂GA（赤霉素）、EBR（表油菜素内酯）、MeJA（茉莉酸甲酯）抑制，但SlGT-24、SlGT-35受ABA（脱落酸）诱导，而SlGT-24还受ACC（1-氨基环丙烷羧酸）诱导。结论番茄GT-1亚家族3个基因受盐、干旱的调控，且对植物生长调节剂的响应明显。该研究为深入探究GT-1亚家族成员的生物功能提供了重要参考。
- 番茄 /
- GT-1基因 /
- 表达模式 /
- 非生物胁迫 /
- 植物生长调节剂诱导
Abstract: Objective Genetic information and functions of trihelix factors in GT-1 critical to the growth, development, and resistance to abiotic stresses and plant growth regulator of tomato plants were investigated. Method Bioinformatic techniques were applied to decipher the bio-evolutionary of GT-1 in tomato. RT-PCR was used to determine the expressions and responses to abiotic stresses and plant growth regulator of the gene. Result (1) In tomato, GT-1 had 3 subfamily members, i.e., SlGT-21, SlGT-24, and SlGT-35. The evolutionary analysis indicated that those members differentiated functionally. (2) The 3 subfamily genes expressed in all tested tomato tissues with the highest level in the fruits, especially during the growing stage. It was speculated that SlGT-21 and SlGT-35 might share some common functionalities. (3) All 3 genes, particularly SlGT-24, could be suppressed by dehydration, and SlGT-21 and SlGT-35 more severely affected by salt stress. (4) The expressions of these genes were inhibited by GA(Gibberellin), EBR(Epihomobrassinolide), and MeJA (Methyl jasmonate); but those of SlGT-24 induced by ABA (Abscisic acid) and ACC(1-Aminocyclopropanecarboxylic acid), and SlGT-35 by ABA. Conclusion Three GT-1 subfamily genes of tomato plants could be regulated by salt and/or dehydration stresses and were sensitive to hormonal stimulations.
- Solanum lycopersicom /
- GT-1 genes /
- expression /
- abiotic stress /
- Plant growth regulator treatments

HTML全文

图 1 GT-1亚家族氨基酸多重序列比对

Figure 1. Multiple alignment of GT-1 protein sequences

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图 2 GT-1亚家族蛋白生物进化分析

Figure 2. Phylogenetic analysis on GT-1 proteins

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图 3 番茄不同组织中GT-1基因的表达模式

R：根；ST：茎； YL：幼叶； ML：成熟叶；SL：老叶； F：花； IMG：未成熟果实； MG：成熟果实；B：破色期； B+4：破色后4 d；B+7：破色后7 d。不同小写字母表示不同番茄组织之间差异显著（P＜0.05）。图4、5同。

Figure 3. Expressions of GT-1 genes in different tissues of AC⁺⁺

RT: roots; ST: stems; YL: young leaves; ML: mature leaves; SL: senescent leaves; FL: flowers; IMG: immature green fruits; MG: mature green fruits; B: color break in fruits; B+4: 4d after color-break; B+7: 7d after color-break. Data with different lowercase letters represent significant differences at P＜0.05. Same for Figs. 4 and 5.

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图 4 干旱处理下GT-1基因的表达模式分析

Figure 4. Expression of GT-1 under dehydration stress

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图 5 盐处理下GT-1基因的表达分析

Figure 5. Expression of GT-1 under salt stress

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图 6 GT-1基因响应植物生长调节剂的表达模式

**代表在 0.01 水平上与对照差异显著，*代表在 0.05 水平上与对照差异显著。

Figure 6. Expressions of GT-1 genes in response to plant growth regulators

**represents significant difference at P＜0.01; *represents significant difference at P＜0.05.

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表 1 研究中所用引物信息

Table 1. Primers applied

引物名称Primer names	序列（5′ - 3′）Sequences（5′ -3′）
qSlCAC-F	CCTCCGTTGTGATGTAACTGG
qSlCAC-R	ATTGGTGGAAAGTAACATCATCG
qSlEF1α-F	TACTGGTGGTTTTGAAGCTG
qSlEF1α-R	AACTTCCTTCACGATTTCATCATA
qSlGT-21-F	GCAATTCGAGGTGAGCTTGAG
qSlGT-21-R	TGTTTCCTTACCCTTGTAACGATT
qSlGT-24-F	TGGAGGTGTTAATATTGGAGGAGG
qSlGT-24-R	TGCATTTACACTGTTCTGGGC
qSlGT-35-F	ACATGGAACCGGTGAGCC
qSlGT-35-R	TGCACGCTTTGTCCTTAATCG

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参考文献(21)

[1]	YU C Y, CAI X F, YE Z B, et al. Genome-wide identification and expression profiling analysis of trihelix gene family in tomato [J]. Biochemical and Biophysical Research Communications, 2015, 468(4): 653−659. doi: 10.1016/j.bbrc.2015.11.010
[2]	KAPLAN-LEVY R N, BREWER P B, QUON T, et al. The trihelix family of transcription factors–light, stress and development [J]. Trends in Plant Science, 2012, 17(3): 163−171. doi: 10.1016/j.tplants.2011.12.002
[3]	FLUHR R, KUHLEMEIER C, NAGY F, et al. Organ-specific and light-induced expression of plant genes [J]. Science, 1986, 232(4754): 1106−1112. doi: 10.1126/science.232.4754.1106
[4]	LIU W, ZHANG Y W, LI W, et al. Genome-wide characterization and expression analysis of soybean trihelix gene family [J]. PeerJ, 2020, 8: e8753. doi: 10.7717/peerj.8753
[5]	HARRISON M J, LAWTON M A, LAMB C J, et al. Characterization of a nuclear protein that binds to three elements within the silencer region of a bean chalcone synthase gene promoter [J]. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(6): 2515−2519. doi: 10.1073/pnas.88.6.2515
[6]	CHATTOPADHYAY S, PUENTE P, DENG X W, et al. Combinatorial interaction of light-responsive elements plays a critical role in determining the response characteristics of light-regulated promoters in Arabidopsis [J]. The Plant Journal:for Cell and Molecular Biology, 1998, 15(1): 69−77. doi: 10.1046/j.1365-313X.1998.00180.x
[7]	LAM E. Domain analysis of the plant DNA-binding protein GT1a: Requirement of four putative alpha-helices for DNA binding and identification of a novel oligomerization region [J]. Molecular and Cellular Biology, 1995, 15(2): 1014−1020. doi: 10.1128/MCB.15.2.1014
[8]	LE GOURRIEREC J, LI Y F, ZHOU D X. Transcriptional activation by Arabidopsis GT-1 may be through interaction with TFIIA-TBP-TATA complex [J]. The Plant Journal:for Cell and Molecular Biology, 1999, 18(6): 663−668. doi: 10.1046/j.1365-313x.1999.00482.x
[9]	HIRATSUKA K, WU X, FUKUZAWA H, et al. Molecular dissection of GT-1 from Arabidopsis [J]. The Plant Cell, 1994, 6(12): 1805−1813.
[10]	LIU X Q, ZHANG H, MA L, et al. Genome-wide identification and expression profiling analysis of the trihelix gene family under abiotic stresses in Medicago truncatula [J]. Genes, 2020, 11(11): 1389. doi: 10.3390/genes11111389
[11]	WANG W L, WU P, LIU T K, et al. Genome-wide analysis and expression divergence of the trihelix family in Brassica rapa: Insight into the evolutionary patterns in plants [J]. Scientific Reports, 2017, 7(1): 1−15. doi: 10.1038/s41598-016-0028-x
[12]	LI K Y, DUAN L L, ZHANG Y B, et al. Genome-wide identification and expression profile analysis of trihelix transcription factor family genes in response to abiotic stress in Sorghum[Sorghum bicolor (L. ) Moench [J]. BMC Genomics, 2021, 22(1): 738. doi: 10.1186/s12864-021-08000-7
[13]	MARÉCHAL E, HIRATSUKA K, DELGADO J, et al. Modulation of GT-1 DNA-binding activity by calcium-dependent phosphorylation [J]. Plant Molecular Biology, 1999, 40(3): 373−386. doi: 10.1023/A:1006131330930
[14]	WANG R, HONG G F, HAN B. Transcript abundance of rml1, encoding a putative GT1-like factor in rice, is up-regulated by Magnaporthe grisea and down-regulated by light [J]. Gene, 2004, 324: 105−115. doi: 10.1016/j.gene.2003.09.008
[15]	PARK H C, KIM M L, KANG Y H, et al. Pathogen- and NaCl-induced expression of the SCaM-4 promoter is mediated in part by a GT-1 box that interacts with a GT-1-like transcription factor [J]. Plant Physiology, 2004, 135(4): 2150−2161. doi: 10.1104/pp.104.041442
[16]	LI N, WEI S T, CHEN J, et al. OsASR2 regulates the expression of a defence-related gene, Os2H16, by targeting the GT-1 cis-element [J]. Plant Biotechnology Journal, 2018, 16(3): 771−783. doi: 10.1111/pbi.12827
[17]	YU C Y, SONG L L, SONG J W, et al. ShCIGT, a Trihelix family gene, mediates cold and drought tolerance by interacting with SnRK1 in tomato [J]. Plant Science, 2018, 270: 140−149. doi: 10.1016/j.plantsci.2018.02.012
[18]	ZHU M K, CHEN G P, ZHANG J L, et al. The abiotic stress-responsive NAC-type transcription factor SlNAC4 regulates salt and drought tolerance and stress-related genes in tomato (Solanum lycopersicum) [J]. Plant Cell Reports, 2014, 33(11): 1851−1863. doi: 10.1007/s00299-014-1662-z
[19]	KOU E F, HUANG X M, ZHU Y N, et al. Crosstalk between auxin and gibberellin during stalk elongation in flowering Chinese cabbage [J]. Scientific Reports, 2021, 11(1): 1−9. doi: 10.1038/s41598-020-79139-8
[20]	YANG Y J, MAO L C, GUAN W L, et al. Exogenous 24-epibrassinolide activates detoxification enzymes to promote degradation of boscalid in cherry tomatoes [J]. Journal of the Science of Food and Agriculture, 2021, 101(6): 2210−2217. doi: 10.1002/jsfa.10840
[21]	CALLEBAUT I, MOSHOUS D, MORNON J, et al. Metallo‐β‐lactamase fold within nucleic acids processing enzymes: The β‐CASP family [J]. Nucleic Acids Research, 2002, 30(16): 3592−3601. doi: 10.1093/nar/gkf470