黄芪茎叶粉对肉牛体外瘤胃发酵参数、产气量及干物质消化率的影响

魏曼丽; 包美丽; 包金花; 任秀珍; 刘天奇; 贾振伟; 魏曼琳

doi:10.19303/j.issn.1008-0384.2023.02.009

黄芪茎叶粉对肉牛体外瘤胃发酵参数、产气量及干物质消化率的影响

doi: 10.19303/j.issn.1008-0384.2023.02.009

魏曼丽^{1, 2},
包美丽¹,
包金花³,
任秀珍^{3, 4},
刘天奇⁵,
贾振伟¹,
魏曼琳^1, ,

1.
内蒙古民族大学动物科技学院，内蒙古　通辽　028000
2.
通辽广播电视台，内蒙古　通辽　028000
3.
内蒙古民族大学农学院，内蒙古　通辽　028000
4.
内蒙古自治区饲用作物工程技术研究中心，内蒙古　通辽　028000
5.
吉林农业大学动物科学技术学院，吉林　长春　130000

基金项目: 内蒙古教育厅高校科研项目（NJZY18156）；内蒙古自治区科技计划项目（2022YFXZ0015）；内蒙古民族大学自治区科技储备项目（2018MDCB04）；内蒙古自治区饲用作物工程技术研究中心资助项目（MDK2016024）；农业农村部农牧交错带牛羊牧繁农育关键技术集成示范项目（16210096）

详细信息

作者简介:
魏曼丽（1983−），女，硕士研究生，研究方向：动物营养与饲料（E-mail：80414165@qq.com）

通讯作者:
魏曼琳（1980−），女，博士，副教授，研究方向：动物营养与饲料（E-mail：weimanlin@163.com）

中图分类号: S823.5
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- 被引次数: 0
出版历程
- 收稿日期: 2022-06-15
- 录用日期: 2022-06-15
- 修回日期: 2022-10-17
- 网络出版日期: 2023-04-14
- 刊出日期: 2023-02-28

Effect of Astragalus Membranaceus Straw on Cattle Rumen Digestion In Vitro

WEI Manli^{1, 2},
BAO Meili¹,
BAO Jinhua³,
REN Xiuzhen^{3, 4},
LIU Tianqi⁵,
JIA Zhenwei¹,
WEI Manlin^{1
, ,}

1.
College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia　028000, China
2.
Tongliao Radio and Television Station, Tongliao,Inner Mongolia　028000, China
3.
College of Agriculture, Inner Mongolia Minzu University, Tongliao, Inner Mongolia　028000, China
4.
Engineering Technology Research Center of Forage Crops, Tongliao, Inner Mongolia　028000, China
5.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin　130000, China

摘要

摘要: 目的采用人工瘤胃法研究黄芪茎叶粉对肉牛体外瘤胃发酵参数、产气量及干物质消化率的影响，以期为黄芪茎叶粉在反刍动物生产中的应用提供理论参考。方法试验分为玉米秸秆对照组（CON组）和黄芪组（AM组）两个处理，每组3个重复，分别于发酵的不同时间点（0、1、2、3、4、5、6、7、8、10、12、14、16、20、24、28、32、36、40、44、48、56、64、72 h）记录两组的产气量，并在发酵72 h后终止发酵，测定发酵液的pH、氨态氮、挥发性脂肪酸和干物质消化率的变化。结果 1）黄芪茎叶粉极显著降低了瘤胃发酵各时间点的产气量和理论最大产气量，但提高了发酵的产气速率（P＜0.01）；2）黄芪茎叶粉显著提高了瘤胃挥发性脂肪酸中乙酸的含量和乙酸/丙酸比值（P＜0.01），降低了氨态氮、总挥发性脂肪酸、丙酸和丁酸含量（P＜0.05）；3）黄芪茎叶粉对瘤胃pH和干物质消化率没有显著影响（P＞0.05）。结论黄芪茎叶粉降低了瘤胃总产气量，对于促进瘤胃乙酸型发酵具有一定调控作用，具有作为反刍动物饲料和饲料添加剂应用的潜力。
- 黄芪 /
- 瘤胃 /
- 产气量 /
- 发酵参数 /
- 干物质消化率
Abstract: Objective Effect of Astragalus membranaceus straw on in vitro rumen digestion of cattle was investigated to determine the applicability of Astragalus membranaceus straw in ruminant feed. Methods An experiment was divided into two groups of corn stalk meal (CON group) and Astragalus membranaceus straw meal (AM group) with three repeats per group. Gas production in the in vitro fermentation at 0, 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 14, 16, 20, 24, 28, 32, 36, 40, 44, 48, 56, 64, and 72 h were monitored. At 72 h, the terminal pH and contents of ammonia nitrogen, volatile fatty acids, and dry matters digestibility were measured. Results (1) In AM group, the amounts of gas production at all sampling points during the fermentation were significantly reduced but the gas production rate was increased (P＜0.01). (2) In AM group, the acetic acid content and the acetic acid to propionic acid ratio were significantly higher (P＜0.01), but the contents of ammonia nitrogen, total volatile fatty acid, propionic acid and butyric acid were lowered significantly (P＜0.05). (3) No significant differences on pH and dry matter digestibility were observed between CON and AM (P＞0.05). Conclusion Astragalus membranaceus straw decreased the total rumen gas production, and had a certain regulatory effect on rumen acetic acid fermentation, which indicated Astragalus membranaceus straw had the potential to be used as feed and feed additive for ruminants.
- Astragalus membranaceus /
- rumen /
- gas production /
- fermentation parameters /
- dry matter digestibility

HTML全文

图 1 黄芪茎叶粉对瘤胃体外发酵产气量的影响

Figure 1. Effect of AM on in vitro gas production

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表 1 玉米秸秆和黄芪茎叶粉中主要营养成分

Table 1. Main nutrients in corn stalk and AM　　　　　　　　　　　　　（单位：%）

指标Items	玉米秸秆Corn stalk	黄芪茎叶粉Astragalus membranaceus straw
干物质Dry matter	85.8	86.5
粗蛋白Crude protein	5.9	10.9
粗脂肪Ether extract	1.3	1.5
粗纤维Crude fiber	35.0	30.2
粗灰分Crude ash	7.0	3.0
无氮浸出物Nitrogen free extract	36.6	40.8
钙Calcium	0.35	1.43
磷Phosphorus	0.19	0.10

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表 2 黄芪茎叶粉对产气量模型参数的影响

Table 2. Effects of AM on parameters of gas production model

指标Items	对照组CON group	黄芪组AM group
12 h产气量 Gas production in 12 hours / mL	28.07±1.65 A	19.07±1.35 B
24 h产气量Gas production in 24 hours / mL	33.17±1.65 A	20.17±1.35 B
48 h产气量Gas production in 48 hours / mL	31.90±2.00 A	19.63±1.65 B
72 h产气量Gas production in 72 hours / mL	33.80±4.30 A	20.53±2.35 B
理论最大产气量B Theoretical maximum gas production/ mL	32.84±2.05 A	20.16±1.98 B
产气速率cGas generation rate/（mL·h⁻¹）	0.16±0.01 b	0.21±0.02 a
表中同行数据后不同大、小写字母表示处理之间差异极显著（P＜0.01）或显著（P＜0.05），相同字母或无字母表示差异不显著（P＞0.05）。下同。In the table, different capital shoulder letters in the table indicate significant differences between groups (P＜0.01), different lowercase letters showed significant difference (P＜0.05), the same letter or no shoulder letters indicates no significant difference (P＞ 0.05), the same as below.

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表 3 黄芪茎叶粉对瘤胃发酵液pH、氨态氮和干物质消化率的影响

Table 3. Effects of AM on rumen pH, ammonia nitrogen, and dry matter digestibility in vitro

指标Items	对照组CON group	黄芪组AM group
pH	6.90±0.10	7.02±0.01
氨态氮Ammonia Nitrogen / （mg·dL⁻¹）	21.70±1.23 a	14.20±4.24 b
干物质消化率Dry matter digestibility/%	63.88±6.46	58.00±0.82

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表 4 黄芪茎叶粉对体外瘤胃挥发性脂肪酸的影响

Table 4. Effects of AM on rumen volatile fatty acids in vitro

指标Item	对照组CON group	黄芪组AM group
总挥发酸Total volatile acid/（mmol·L⁻¹）	28.33±0.74 a	15.83±0.35 b
乙酸 Acetate/%	65.03±0.40 B	69.63±0.30 A
丙酸 Propionic acid/%	19.17±0.10 A	17.70±0.17 B
丁酸 Butyrate/%	12.30±0.20 A	9.10±0.06 B
戊酸 Valerate/%	0.90±0.25	0.77±0.06
异丁酸 Isobutyrate/%	0.87±0.56	1.07±0.10
异戊酸 Isovalerate/%	1.80±0.06	1.80±0.06
乙酸/丙酸The ratio of acetate acid to propionic acid	3.43±5.35 B	3.93±5.47 A

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参考文献(50)

[1]	MCGUFFEY R K, RICHARDSON L F, WILKINSON J I D. Ionophores for dairy cattle: Current status and future outlook [J]. Journal of Dairy Science, 2001, 84: E194−E203. doi: 10.3168/jds.S0022-0302(01)70218-4
[2]	WANG X J, DING L M, WEI H Y, et al. Astragalus membranaceus root supplementation improves average daily gain, rumen fermentation, serum immunity and antioxidant indices of Tibetan sheep [J]. Animal, 2021, 15(1): 100061. doi: 10.1016/j.animal.2020.100061
[3]	谢静, 龚易昕悦, 丁立生, 等. 黄芪及其活性成分对脓毒症临床和药理作用的研究进展 [J]. 中草药, 2021, 52(8):2502−2510. XIE J, GONG Y X Y, DING L S, et al. Progress in clinical and pharmacological studies of Astragali Radix and its active components against sepsis [J]. Chinese Traditional and Herbal Drugs, 2021, 52(8): 2502−2510.(in Chinese)
[4]	HUANG H, LUO S H, HUANG D C, et al. Immunomodulatory activities of proteins from Astragalus membranaceus waste [J]. Journal of the Science of Food and Agriculture, 2019, 99(8): 4174−4181. doi: 10.1002/jsfa.9650
[5]	张王宁, 高耀, 李科, 等. 基于网络药理学的黄芪总黄酮治疗肾病综合征的机制研究 [J]. 药学学报, 2018, 53(9):1429−1441. ZHANG W N, GAO Y, LI K, et al. Exploration into the mechanism of total flavonoids of Astragali Radix in the treatment of nephrotic syndrome based on network pharmacology [J]. Acta Pharmaceutica Sinica, 2018, 53(9): 1429−1441.(in Chinese)
[6]	李紫岩, 朱寿东, 刘澜波, 等. 内蒙古道地药材蒙古黄芪生态适宜性区划研究 [J]. 中国农业科技导报, 2021, 23(2):170−176. LI Z Y, ZHU S D, LIU L B, et al. Research on the ecological suitability division of Astragalus membranaceus (Fisch. ) bge. var. mongholicus (Bge. ) P. K. Hsiao in Inner Mongolia [J]. Journal of Agricultural Science and Technology, 2021, 23(2): 170−176.(in Chinese)
[7]	ZHANG J Q, XIE X S, LI C, et al. Systematic review of the renal protective effect of Astragalus membranaceus (root) on diabetic nephropathy in animal models [J]. Journal of Ethnopharmacology, 2009, 126(2): 189−196. doi: 10.1016/j.jep.2009.08.046
[8]	申义君, 周金伟, 王斌, 等. 黄芪多糖对泌乳期奶牛抗氧化能力的影响 [J]. 天然产物研究与开发, 2014, 26(2):244−247. SHEN Y J, ZHOU J W, WANG B, et al. Effects of Astragalus polysaccharides on the activity of antioxidant enzyme in lactating Holstein cows [J]. Natural Product Research and Development, 2014, 26(2): 244−247.(in Chinese)
[9]	郭思凡, 曹杰. 黄芪多糖、银黄联用对新生犊牛免疫力及发病的影响 [J]. 中国乳业, 2021(10):61−66. doi: 10.12377/1671-4393.21.10.13 GUO S F, CAO J. Effects of Astragalus polysaccharide combined with Yinhuang soluble powder on immunity and diseases in neonatal calves [J]. China Dairy, 2021(10): 61−66.(in Chinese) doi: 10.12377/1671-4393.21.10.13
[10]	王义翠, 刘延鑫, 孙宇, 等. 不同水平黄芪多糖对早期断奶犊牛生长性能及抗氧化能力的影响 [J]. 黑龙江畜牧兽医, 2018(20):151−153,156. WANG Y C, LIU Y X, SUN Y, et al. Effects of different levels of Astragalus polysaccharides on growth performance andantioxidant capacity in early weaned calves [J]. Heilongjiang Animal Science and Veterinary Medicine, 2018(20): 151−153,156.(in Chinese)
[11]	王宪举. 饲喂黄芪对藏羊生长、生理代谢和肉品质的影响[D]. 兰州: 兰州大学, 2020. WANG X J. The effect of supplementing Astragalus membranaceus on growing performance, physiological metabolism and meat quality of Tibetan sheep[D]. Lanzhou: Lanzhou University, 2020. (in Chinese)
[12]	魏海燕, 王宪举, 闫琦, 等. 添加黄芪超细粉和抗生素对瘤胃体外发酵的影响 [J]. 家畜生态学报, 2020, 41(7):23−30. WEI H Y, WANG X J, YAN Q, et al. Effect of Astragalus superfine powder and antibiotics on in vitro rumen fermentation [J]. Journal of Domestic Animal Ecology, 2020, 41(7): 23−30.(in Chinese)
[13]	郭丹丹, 孔庆娟, 申子平. 枯草芽孢杆菌发酵黄芪对断奶仔猪生长性能和血液指标的影响 [J]. 饲料研究, 2019, 42(3):91−94. GUO D D, KONG Q J, SHEN Z P. Effects of Bacillus subtilis fermented astragalus on growth performance and blood biochemical indices of weaned piglets [J]. Feed Research, 2019, 42(3): 91−94.(in Chinese)
[14]	张国庆. 黄芪茎叶的利用研究 [J]. 天津药学, 2010, 22(5):5−7. ZHANG G Q. Investigation of active ingredients from roots and stem Leaves of Astragalus mongholicus Bunge [J]. Tianjin Pharmacy, 2010, 22(5): 5−7.(in Chinese)
[15]	李伟, 赵金波, 孟维珊, 等. 黄芪茎叶对犊牛生长影响的研究 [J]. 现代畜牧科技, 2022(3):14−15,59. doi: 10.19369/j.cnki.2095-9737.2022.03.005 LI W, ZHAO J B, MENG W S, et al. Effect of Astragalus membranaceus stems and leaves on calf growth [J]. Modern Animal Husbandry Science & Technology, 2022(3): 14−15,59.(in Chinese) doi: 10.19369/j.cnki.2095-9737.2022.03.005
[16]	朱正兰, 江蔚新. 黄芪地上部分有效成分研究 [J]. 黑龙江医药, 2004, 17(6):417−419. doi: 10.3969/j.issn.1006-2882.2004.06.008 ZHU Z L, JIANG W X. Study on the effective components in the overground organ of membranaceus [J]. Heilongjiang Medical Journal, 2004, 17(6): 417−419.(in Chinese) doi: 10.3969/j.issn.1006-2882.2004.06.008
[17]	佟继铭, 陈光晖, 刘玉玲, 等. 黄芩茎叶总黄酮对家兔实验性动脉粥样硬化的预防作用 [J]. 中草药, 2005, 36(1):93−95. TONG J M, CHEN G H, LIU Y L, et al. Preventive effect of total flavonoids from stems and leaves of Scutellaria baicalensis Georgi on experimental atherosclerosis in rabbits [J]. Chinese Traditional and Herbal Drugs, 2005, 36(1): 93−95.(in Chinese)
[18]	LI Y, GUO S, ZHU Y, et al. Flowers of Astragalus membranaceus var. mongholicus as a novel high potential by-product: Phytochemical characterization and antioxidant activity [J]. Molecules (Basel, Switzerland), 2019, 24(3): 434. doi: 10.3390/molecules24030434
[19]	周庆民, 张艳, 徐馨, 等. 黄芪茎叶生物发酵物对奶牛应用效果的试验研究 [J]. 中国乳业, 2016(9):32−35. ZHOU Q M, ZHANG Y, XU X, et al. Experimental study on application effect of biological fermentation products of Astragalus membranaceus stems and leaves on dairy cows [J]. China Dairy, 2016(9): 32−35.(in Chinese)
[20]	MENKE K H, RAAB L, SALEWSKI A, et al. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro [J]. The Journal of Agricultural Science, 1979, 93(1): 217−222.
[21]	ØRSKOV E R, MCDONALD I. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage [J]. The Journal of Agricultural Science, 1979, 92(2): 499−503. doi: 10.1017/S0021859600063048
[22]	冯宗慈, 高民. 通过比色测定瘤胃液氨氮含量方法的改进 [J]. 畜牧与饲料科学, 2010, 31(S1):37. FENG Z C, GAO M. Improvement of determination of ammonia nitrogen content in rumen fluid by colorimetric method [J]. Animal Husbandry and Feed Science, 2010, 31(S1): 37.(in Chinese)
[23]	ERWIN E S, MARCO G J, EMERY E M. Volatile fatty acid analyses of blood and rumen fluid by gas chromatography [J]. Journal of Dairy Science, 1961, 44(9): 1768−1771. doi: 10.3168/jds.S0022-0302(61)89956-6
[24]	王芳, 徐元君, 牛俊丽, 等. 体外产气法评价反刍动物饲料营养价值的研究 [J]. 中国畜牧兽医, 2016, 43(1):76−83. WANG F, XU Y J, NIU J L, et al. Nutrient value evaluation of different type feeds by in vitro gas production method [J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(1): 76−83.(in Chinese)
[25]	付瑶, 史仁煌, 王雅晶, 等. 体外法研究不同水平中性洗涤纤维日粮对泌乳高峰期奶牛瘤胃发酵和产气量的影响 [J]. 中国畜牧杂志, 2016, 52(7):46−50. FU Y, SHI R H, WANG Y J, et al. Effects of NDF level in diet on rumen fermentation and gas production in vitro during peak lactation period of dairy cows [J]. Chinese Journal of Animal Science, 2016, 52(7): 46−50.(in Chinese)
[26]	朱丹, 张佩华, 赵勐, 等. 不同中性洗涤纤维与淀粉比例饲粮对体外瘤胃发酵的影响 [J]. 动物营养学报, 2015, 27(8):2580−2588. ZHU D, ZHANG P H, ZHAO M, et al. Effects of different neutral detergent fiber to starch raito diets on rumen fermentation in vitro [J]. Chinese Journal of Animal Nutrition, 2015, 27(8): 2580−2588.(in Chinese)
[27]	马艳艳, 成艳芬, 朱伟云. 体外发酵法评价不同产地、不同茬次苜蓿的营养价值 [J]. 动物营养学报, 2014, 26(8):2421−2432. MA Y Y, CHENG Y F, ZHU W Y. Different origins and stubbles of alfalfa: Evaluation of nutritional value by an in vitro fermentation method [J]. Chinese Journal of Animal Nutrition, 2014, 26(8): 2421−2432.(in Chinese)
[28]	毕思思. 黄芪属植物对牦牛与黄牛体外发酵特征的影响[D]. 兰州: 兰州大学, 2019. BI S S. Effects of Astragalus plants on in vitro fermentation characteristics of yak and cattle[D]. Lanzhou: Lanzhou University, 2019. (in Chinese)
[29]	张芮铭, 田可, 何天乐, 等. 植物提取物对高精料饲粮下反刍动物瘤胃功能、微生物区系和炎症反应的调控效果 [J]. 动物营养学报, 2020, 32(1):36−41. doi: 10.3969/j.issn.1006-267x.2020.01.006 ZHANG R M, TIAN K, HE T L, et al. Regulation effects of plant extracts on rumen function, microflora and inflammatory response in ruminants under high-concentrate diet [J]. Chinese Journal of Animal Nutrition, 2020, 32(1): 36−41.(in Chinese) doi: 10.3969/j.issn.1006-267x.2020.01.006
[30]	毕小凤. 黄芪茎叶主要化学成分及其部分药理作用研究[D]. 太原: 山西中医药大学, 2017. BI X F. Studies on chemical constituents and pharmacological effects of stem leaf of Astragalus membranacleus[D]. Taiyuan: Shanxi University of Traditional Chinese Medicine, 2017. (in Chinese)
[31]	曹洪志, 李成贤, 李雪梅, 等. 竹叶提取物对肉牛生产性能、瘤胃发酵特征及微生物数量的影响 [J]. 饲料研究, 2022, 45(1):15−19. CAO H Z, LI C X, LI X M, et al. Effects of bamboo leaf extract on production performance, rumen fermentation characteristics and rumen microbial populations of beef cattle [J]. Feed Research, 2022, 45(1): 15−19.(in Chinese)
[32]	杨鼎, 晔薷罕, 李长青, 等. 槲皮素等类黄酮对瘤胃微生物影响研究进展 [J]. 畜牧与饲料科学, 2020, 41(6):32−36,45. doi: 10.12160/j.issn.1672-5190.2020.06.006 YANG D, YE R H, LI C Q, et al. Research advances on the effects of quercetin and other flavonoids on rumen microorganisms [J]. Animal Husbandry and Feed Science, 2020, 41(6): 32−36,45.(in Chinese) doi: 10.12160/j.issn.1672-5190.2020.06.006
[33]	WINA E. Saponins: Effects on Rumen Microbial Ecosystem and Metabolism in the Rumen[M]//PATRA A. Dietary Phytochemicals and Microbes. Dordrecht: Springer, 2012: 311-350.
[34]	焦阳, 叶均安. 皂甙调控反刍动物瘤胃发酵和生产性能的研究进展 [J]. 中国畜牧杂志, 2015, 51(9):72−76. doi: 10.3969/j.issn.0258-7033.2015.09.016 JIAO Y, YE J A. Regulation of saponins on rumen fermentation and productive performance in ruminants [J]. Chinese Journal of Animal Science, 2015, 51(9): 72−76.(in Chinese) doi: 10.3969/j.issn.0258-7033.2015.09.016
[35]	张清月. 诺丽果多糖对绒山羊瘤胃发酵、营养物质消化、免疫及抗氧化功能的影响[D]. 呼和浩特: 内蒙古农业大学, 2019. ZHANG Q Y. Effects of noni fruit polysaccharide on rumen fermentation, nutrient digestion, immunity and antioxidation in Cashmere goats[D]. Hohhot: Inner Mongolia Agricultural University, 2019. (in Chinese)
[36]	魏海燕. 饲喂黄芪对断乳牦牛犊生长和生理代谢的影响[D]. 兰州: 兰州大学, 2020. WEI H Y. The effect of supplementing Astragalus to weaned yak calves on growth performance and physiological metabolism[D]. Lanzhou: Lanzhou University, 2020. (in Chinese)
[37]	LIANG X, YAMAZAKI K, KAMRUZZAMAN M, et al. Effects of Chinese herbal medicine on plasma glucose, protein and energy metabolism in sheep [J]. Journal of Animal Science and Biotechnology, 2013, 4(1): 51. doi: 10.1186/2049-1891-4-51
[38]	祜群, 宝拉日, 海林, 等. 中草药添加剂对奶牛干物质采食量和瘤胃发酵的影响 [J]. 中国饲料, 2018(18):38−42. doi: 10.15906/j.cnki.cn11-2975/s.20181808 HU Q, BAO L R, HAI L, et al. Effects of herbal additives on dry matter intake and rumen fermentation in dairy cows [J]. China Feed, 2018(18): 38−42.(in Chinese) doi: 10.15906/j.cnki.cn11-2975/s.20181808
[39]	邓必贤, 邓文琼, 李健, 等. 黄芪超微粉对三黄鸡小肠绒毛形态结构、肠内容物消化酶活性和盲肠菌群的影响 [J]. 福建农林大学学报(自然科学版), 2014, 43(6):616−621. doi: 10.13323/j.cnki.j.fafu(nat.sci.).2014.06.010 DENG B X, DENG W Q, LI J, et al. Effects of Astragalus ultramicro powder on the small intestinal villus structure, intestinal contents activities of digestive enzymes and cecum microflora of three yellow broilers [J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2014, 43(6): 616−621.(in Chinese) doi: 10.13323/j.cnki.j.fafu(nat.sci.).2014.06.010
[40]	NAMKUNG H, LI J GONG M, YU H, et al. Impact of feeding blends of organic acids and herbal extracts on growth performance, gut microbiota and digestive function in newly weaned pigs [J]. Canadian Journal of Animal Science, 2004, 84(4): 697−704. doi: 10.4141/A04-005
[41]	冯士彬, 程连平, 舒迎霜, 等. 黄芪多糖对湖羊羔羊生长性能、血清指标、消化功能和直肠菌群的影响 [J]. 江苏农业学报, 2019, 35(1):122−129. doi: 10.3969/j.issn.1000-4440.2019.01.018 FENG S B, CHENG L P, SHU Y S, et al. Effect of astragalus polysaccharide on growth performance, serum indices, digestive function and rectum flora of Hu lambs [J]. Jiangsu Journal of Agricultural Sciences, 2019, 35(1): 122−129.(in Chinese) doi: 10.3969/j.issn.1000-4440.2019.01.018
[42]	ZHOU T X, ZHANG Z F, KIM I H. Effects of dietary Coptis chinensis herb extract on growth performance, nutrient digestibility, blood characteristics and meat quality in growing-finishing pigs [J]. Asian-Australasian Journal of Animal Sciences, 2013, 26(1): 108−115. doi: 10.5713/ajas.2011.11400
[43]	LIU C, QU Y H, GUO P T, et al. Effects of dietary supplementation with alfalfa (Medicago sativa L. ) saponins on lamb growth performance, nutrient digestibility, and plasma parameters [J]. Animal Feed Science and Technology, 2018, 236: 98−106. doi: 10.1016/j.anifeedsci.2017.12.006
[44]	EL-ASHRY M A, EL-BORDENY N E, KHATTAB H M, et al. Effect of dietary supplemented with medicinal herbs on nutrient digestibility and some blood metabolites of buffalo calves [J]. Egyptian Journal of Nutritional and Feeds, 2006, 9(2): 179−191.
[45]	SEKIROV I, RUSSELL S L, ANTUNES L C, et al. Gut microbiota in health and disease [J]. Physiological Reviews, 2010, 90(3): 859−904. doi: 10.1152/physrev.00045.2009
[46]	ABDALLAH A, ZHANG P, ABUBAKARI A H, et al. Reclamation of Astragalus by-product through dietary inclusion in ruminant diets: Effects on growth performance, nutrient digestibility, rumen fermentation, blood biochemical parameters, and humoral immune response in sheep [J]. Evidence-Based Complementary and Alternative Medicine:ECAM, 2019, 2019: 8530961.
[47]	顾小卫, 赵国琦, 金晓君, 等. 中草药添加剂对奶牛干物质采食量及瘤胃内环境的影响 [J]. 中国奶牛, 2010(4):18−21. doi: 10.3969/j.issn.1004-4264.2010.04.009 GU X W, ZHAO G Q, JIN X J, et al. Effects of feeding herbal additives on dry matter intake and the ruminal environment in dairy cows [J]. China Dairy Cattle, 2010(4): 18−21.(in Chinese) doi: 10.3969/j.issn.1004-4264.2010.04.009
[48]	王小晶. 不同日粮中添加酸化剂、植物提取物和中草药对山羊体外发酵及甲烷产量的影响[D]. 重庆: 西南大学, 2009. WANG X J. Effects of acidifiers, plant extracts and Chinese herbal medicine on in vitro rumen fermentation and methane emission in goats[D]. Chongqing: Southwest University, 2009. (in Chinese)
[49]	HOLMES E, LI J, MARCHESI J, et al. Gut microbiota composition and activity in relation to host metabolic phenotype and disease risk [J]. Cell Metabolism, 2012, 16(5): 559−564. doi: 10.1016/j.cmet.2012.10.007
[50]	郭晨阳. 植物复合提取物对奶牛瘤胃发酵、产奶性能和细菌区系的影响[D]. 呼和浩特: 内蒙古农业大学, 2020 GUO C Y. Effects of compound plant extracts on rumen fermentation, milk production performance and bacterial flora in dairy cows[D]. Hohhot: Inner Mongolia Agricultural University, 2020. (in Chinese)