脱氧雪腐镰刀菌烯醇（DON）作为激发子对马铃薯土传病害的防治效果研究

魏旭红; 冯旭; 贺付蒙; 徐永清; 李建颖; 肖萌萌; 李丽; 李翠婷; 袁强; 李凤兰

doi:10.19303/j.issn.1008-0384.2023.03.009

脱氧雪腐镰刀菌烯醇（DON）作为激发子对马铃薯土传病害的防治效果研究

doi: 10.19303/j.issn.1008-0384.2023.03.009

东北农业大学生命科学学院，黑龙江　哈尔滨　150006

基金项目: 国家自然科学基金项目（31201470）；中国科学院黑土地保护与利用科技创新工程专项（XDA28030302-1）；阜阳师范大学承接阜阳市科技专项（SXHZ202007）

详细信息

作者简介:
魏旭红（1995−），女，硕士研究生，研究方向：植物资源与分子生物学（E-mail：2311781147@qq.com）

通讯作者:
李凤兰（1973−），女，博士后，教授，研究方向：植物资源与分子生物学（E-mail：lifenglan@neau.edu.cn）

中图分类号: Q945.8
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出版历程
- 收稿日期: 2022-11-08
- 修回日期: 2022-11-30
- 网络出版日期: 2023-02-08
- 刊出日期: 2023-03-28

Efficacy of Vomitoxin as Elicitor for Controlling Soilborne Diseases on Potato Plants

College of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang　150006, China

摘要

摘要: 目的验证脱氧雪腐镰刀菌烯醇（DON）的安全性，探讨DON作为激发子在马铃薯主要土传病害防治中的应用效果。方法以大西洋品种马铃薯为研究对象，筛选DON作为激发子诱导抗病的最适质量浓度以及最佳处理时间，优化DON诱导马铃薯抗病的条件，检测生理指标的变化。结果结果显示低质量浓度下DON处理对马铃薯植株均无显著药害；5 ng·mL⁻¹ DON处理4 h可以有效抑制青枯病致病菌茄科雷尔氏菌、软腐病致病菌菊欧氏菌、疮痂病致病菌疮痂链霉菌在马铃薯叶片中的扩展，3 ng·mL⁻¹ DON处理4 h可以有效抑制干腐病致病菌接骨木镰刀菌在马铃薯叶片组织中的扩展；当温度为25 ℃、pH为7时DON诱导马铃薯抗青枯病、干腐病、软腐病效果最好，温度为25 ℃、pH为9时DON诱导马铃薯抗疮痂病效果最好；经DON诱导处理的马铃薯叶片在光照强度为12 000 lx条件下诱导抗干腐病效果最好，在光照强度为15 000 lx条件下诱导抗青枯病、软腐病、疮痂病效果最好；DON处理马铃薯叶片后，提高了SOD酶活性，同时，木质素含量增加，MDA含量减少。结论低质量浓度的DON作为激发子处理马铃薯叶片后对马铃薯无显著药害，在马铃薯主要土传病害防治中的应用效果较显著，可为马铃薯土传病害的预防和治理提供理论依据和新的思路。
- DON /
- 激发子 /
- 土传病害 /
- 优化 /
- 生理指标
Abstract: Objective Safety and efficacy of applying vomitoxin to control soilborne diseases on potato plants were investigated. Method In a laboratory experiment, vomitoxin (aka deoxynivalenol, DON) was applied on the Atlantic variety potato to determine the disease resistance to and physiological alternations induced by some major soilborne pathogens on the plant for treatment optimization. Result Within a range of concentrations, DON caused no significant ill-effects on the potato plants. A treatment of it at 5 ng·mL⁻¹ for 4 h effectively inhibited the spread of bacterial wilt caused by Ralstonia solanacearum, soft rots by Erwinia chrysanthemi, or scabies by Streptomyces scabies on the leaves, and at a dosage of 3 ng·mL⁻¹, the growth of dry rot pathogen Fusarium sambucinum was retarded. The strongest DON-induced resistance against the bacterial wilt or dry and soft rots of the plant was found at 25 ℃ and pH 7, and against scabies at pH 9. With an exposure of 12 000 lx light, the DON-induction on the plants reached the greatest efficacy against the dry rots. Whereas, under 15 000 lx, that could be achieved on the bacterial wilt, soft rots, and scabies. A DON treatment significantly raised the SOD activity and lignin content but lowered MDA in the leaves. Conclusion Application of DON as an elicitor in low concentrations exerted no adverse effect but significantly inhibit some major soilborne diseases on potato plants. A potential venue for preventing and treating the diseases seemed merit further investigation.
- DON /
- elicitor /
- soilborne diseases /
- optimization /
- physiological indices

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图 1 不同质量浓度DON对马铃薯叶片接种致病菌病斑直径的影响

①图中字母A–D分别表示：茄科雷尔氏菌、接骨木镰刀菌、菊欧氏菌、疮痂链霉菌。图2~5同。②不同小写字母表示差异显著（P＜0.05）。图2~5同。

Figure 1. Effect of DON in varied concentrations on diameter of diseased spot on infected potato leaves

① A–D: R. solanacearum, F. sambucinum, E. chrysanthemi, and S. scabiesscab. Same for Fig.2–5. ② Data with different lowercase letters indicate significant differences (P＜0.05). Same for Fig.2–5.

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图 2 DON处理不同时间对马铃薯叶片接种致病菌病斑大小的影响

Figure 2. Effect of DON treatment time on size of diseased spot on infected potato leaves

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图 3 不同pH的DON处理对马铃薯叶片接种致病菌病斑大小的影响

Figure 3. Effect of DON treatment at different pH on size of diseased spot on infected potato leaves

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图 4 温度对DON诱导马铃薯抗疮痂病效果的影响

Figure 4. Effect of temperature on DON-induced scab-resistance of potato plant

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图 5 光照强度对DON诱导马铃薯抗病效果的影响

Figure 5. Effect of light intensity on DON-induced scab-resistance of potato plant

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表 1 试验仪器与试剂

Table 1. Instruments and reagents applied for experiment

序号 Serial number	名称 Name	型号 Model number	公司 Company
1	电子天平	ML204	梅特勒-托利多仪器有限公司
2	高压灭菌锅	QC-SOE	山东新华医疗器械股份有限公司
3	摇床	SPH-211B-GZ	上海世单实验设备有限公司
4	高速台式离心机	TCL-16	上海子期实验设备有限公司
5	光照培养箱	BSG-400	上海博迅医疗生物仪器公司
6	电热恒温培养箱	DRP-9162	上海森信实验仪器有限公司
7	4 ℃离心机	MIkro-220R	德祥科技有限公司
8	−80 ℃冰箱	U410-86	济南光耀医疗设备有限公司
9	pH计	PHS-3C	上海高致精密仪器公司
10	−20 ℃冰箱	BCD-116	广州金松电器营销有限公司
11	酶标仪		上海科华实验系统有限公司
12	丙二醛测定试剂盒		南京建成生物工程研究所
13	SOD活性检测试剂盒		索莱宝生物科技有限公司
14	木质素含量检测试剂盒		索莱宝生物科技有限公司

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表 2 DON处理对马铃薯生长的影响

Table 2. Effect of DON treatment on potato growth

处理 Treatments	平均株高 Average plant height /cm	生长速率 Growth rate/(cm·d⁻¹)		变色率 Discoloration rate/%	坏死率 Necrosis rate/%
处理 Treatments	平均株高 Average plant height /cm	处理7 d后 7 d after treatment	处理14 d后 14 d after treatment	变色率 Discoloration rate/%	坏死率 Necrosis rate/%
CK	24.53	0.88±0.36 a	0.65±0.47 a	—	—
5 ng·mL⁻¹DON	25.06	0.93±0.48 a	0.62±0.45 a	0	0
10 ng·mL⁻¹DON	26.05	0.92±0.42 a	0.64±0.40 a	0	0
15 ng·mL⁻¹DON	23.12	0.87±0.42 a	0.66±0.43 a	0	0
20 ng·mL⁻¹DON	27.80	0.92±0.38 a	0.64±0.41 a	0	0
①“—”为对照组的变色率、坏死率，无数据。②同列数据后不同小写字母表示差异显著 (P＜0.05)。 ①"—" refers to discoloration and necrosis rates of control, no data available.②Data with different lowercase letters on the same column indicate significant differences (P＜0.05).

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表 3 DON处理马铃薯叶片SOD酶活性的变化

Table 3. SOD activity in potato leaves treated by DON （U·g⁻¹）

处理 Treatment	时间 Time/h
处理 Treatment	0	6	12	24	48
CK	97.89±7.53 de	106.73±15.30 e	111.04±10.16 f	122.00±13.11 d	116.44±6.5 c
DON	93.06±5.15 e	130.12±10.00 bcd	146.74±5.84 bcd	167.08±5.06 ab	144.79±12.37 b
RS	94.40±13.02 e	112.79±6.30 de	122.03±13.74 ef	130.00±10.00 cd	134.66±5.03 b
RS+DON	138.52±13.69 ab	149.44±12.74 ab	164.21±11.99 ab	155±15.00 ab	136.79±7.01 b
F. Samb	112.96±6.12 cd	113.85±5.38 de	116.53±18.11 ef	129.35±10.06 cd	135.16±5.01 b
F. Samb+DON	122.26±5.16 bc	140.82±8.80 abc	151.23±9.18 abc	159.67±8.96 ab	165.57±6.08 a
ECH	102.63±5.54 de	123.13±11.37 cde	136.03±15.51 cde	149.11±12.49 bc	135.54±5.09 b
ECH+DON	140.3±13.06 a	138.83±7.82 abc	165.41±15.02 ab	173.74±6.48 a	136.79±7.01 b
SS	99.30±9.51 de	129.21±7.89 cd	127.03±15.41 def	128.11±10.12 d	110.06±12.67 c
SS+DON	135.8±17.13 ab	154.22±20.00 a	170.52±7.62 a	161.03±20.00 ab	134.26±10.01 b
①表中CK、DON分别表示：无菌水、5 ng·mL⁻¹ DON，下同。②RS、F. Samb、ECH、SS分别表示：茄科雷尔氏菌、接骨木镰刀菌、菊欧氏菌、疮痂链霉菌，下同。③RS+DON、F. Samb+DON、ECH+DON、SS+DON分别表示：接菌并施加DON处理，下同。④不同小写字母表示差异显著（P＜0.05），下同。 ① CK、DON: Sterile water, 5 ng·mL⁻¹ DON, same for below. ② RS、F. Samb、ECH、SS: R. solanacearum, F. sambucinum, E. chrysanthemi, S. scabies, same for below. ③ RS+DON、F. Samb+DON、ECH+DON、SS+DON: Inoculate and apply DON, same for below. ④ Data with different lowercase letters indicate significant differences (P＜0.05), same for below.

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表 4 DON处理马铃薯叶片MDA含量的变化

Table 4. MDA content in potato leaves treated by DON （nmol·mg⁻¹）

处理 Treatment	时间 Time/h
处理 Treatment	0	6	12	24	48
CK	1.24±0.05 a	1.31±0.17 a	1.28±0.06 a	1.36±0.14 a	1.15±0.14 a
DON	1.18±0.20 ab	1.12±0.14 ab	1.09±0.07 ab	1.00±0.12 bc	0.97±0.04 ab
RS	1.25±0.11 a	1.04±0.13 bc	0.83±0.05 c	1.07±0.16 b	0.99±0.01 ab
RS+DON	0.91±0.17 cd	0.84±0.18 cd	0.94±0.20 bc	0.81±0.14 c	0.80±0.16 bc
F. Samb	1.11±0.22 abc	1.04±0.13 bc	1.25±0.11 a	0.91±0.10 bc	0.94±0.22 abc
F. Samb+DON	0.83±0.09 d	0.73±0.10 d	0.94±0.20 bc	0.77±0.10 c	0.67±0.12 c
ECH	1.18±0.09 ab	1.04±0.12 bc	0.92±0.06 bc	1.08±0.18 b	1.01±0.20 ab
ECH+DON	0.92±0.17 bcd	0.85±0.17 cd	0.79±0.17 c	0.88±0.10 bc	0.88±0.15 abc
SS	1.22±0.15 a	0.90±0.04 bcd	1.24±0.14 a	1.00±0.10 bc	1.08±0.10 ab
DON+SS	0.90±0.04 cd	0.81±0.17 cd	0.94±0.20 bc	0.87±0.10 bc	0.80±0.16 bc

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表 5 DON处理马铃薯叶片木质素含量的变化

Table 5. Lignin content in potato leaves treated by DON （mg·g⁻¹）

处理 Treatment	时间 Time/h
处理 Treatment	0	6	12	24	48
CK	0.58±0.06 c	0.59±0.08 d	0.65±0.05 c	0.67±0.08 d	0.73±0.04 c
DON	0.64±0.11 bc	0.74±0.06 bc	0.90±0.04 ab	0.92±0.07 bc	0.89±0.07 b
RS	0.69±0.05 bc	0.75±0.09 bc	0.81±0.12 bc	0.89±0.12 c	0.89±0.09 b
RS+DON	0.91±0.06 a	0.99±0.05 a	1.05±0.08 a	1.10±0.08 a	1.12±0.11 a
F. Samb	0.69±0.08 bc	0.77±0.08 bc	0.82±0.15 bc	0.85±0.06 c	0.88±0.10 b
F. Samb+DON	0.91±0.08 a	1.00±0.10 a	1.07±0.14 a	1.11±0.12 a	1.15±0.11 a
ECH	0.65±0.12 bc	0.69±0.05 cd	0.80±0.12 bc	0.77±0.17 cd	0.83±0.10 bc
ECH+DON	0.91±0.10 a	1.02±0.03 a	1.07±0.12 a	1.14±0.09 a	1.12±0.08 a
SS	0.71±0.06 b	0.82±0.07 b	0.80±0.19 bc	0.78±0.10 cd	0.74±0.06 c
SS+DON	1.01±0.03 a	1.04±0.07 a	1.07±0.12 a	1.08±0.03 ab	1.10±0.08 a

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