宁夏引黄灌区盐碱富硒土壤对水稻硒元素吸收积累的影响

唐玙璠; 张智; 康淑铭; 张恩; 马东海; 谢小伟; 许兴; 王彬

doi:10.19303/j.issn.1008-0384.2023.07.013

宁夏引黄灌区盐碱富硒土壤对水稻硒元素吸收积累的影响

doi: 10.19303/j.issn.1008-0384.2023.07.013

唐玙璠^{1, 2,},
张智^{1, 2},
康淑铭¹,
张恩¹,
马东海¹,
谢小伟¹,
许兴¹,
王彬^1, ,

1.
宁夏大学农学院，宁夏　银川　750021
2.
宝鸡市农业科学研究院，陕西　宝鸡　722400

基金项目: 宁夏回族自治区自然科学基金项目（2022AAC03060）

详细信息

作者简介:
唐玙璠（1995 —），女，硕士研究生，主要从事盐碱地富硒功能农业研究，E-mail：tangyufann@126.com

通讯作者:
王彬（1977 —），男，博士，教授，主要从事植物逆境生理研究，E-mail: wb_y2004@nxu.edu.cn

中图分类号: S511
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出版历程
- 收稿日期: 2022-12-28
- 修回日期: 2023-04-28
- 网络出版日期: 2023-08-16
- 刊出日期: 2023-07-28

Effects of Salinity and Alkalinity of Selenium-rich Soils in Yellow River Irrigation Area of Ningxia on Selenium Absorption and Quality of Rice

TANG Yufan^{1, 2
,},
ZHANG Zhi^{1, 2},
KANG Shuming¹,
ZHANG En¹,
MA Donghai¹,
XIE Xiaowei¹,
XU Xing¹,
WANG Bin^{1
, ,}

1.
College of Agriculture, Ningxia University, Yinchuan, Ningxia　750021, China
2.
Baoji Academy of Agricultural Sciences, Baoji, Shaanxi　722400,China

摘要

摘要: 目的探明不同类型盐碱富硒土壤对水稻硒的吸收、转运积累的影响。方法以耐盐碱水稻宁粳45号为试验材料，设置3种不同类型盐碱富硒土壤：A处理（轻度盐化土壤）、B处理（轻度碱化土壤）、C处理（中重度碱化土壤），测定水稻各生育时期（苗期、拔节期、抽穗期、灌浆期、成熟期）根、茎、叶、籽粒、麸皮的硒含量。结果（1）水稻根、茎、叶和籽粒中的硒含量均表现为：C处理＞B处理＞A处理。成熟期硒含量高低呈现根系＞茎＞叶＞籽粒的特点。成熟期籽粒均达到富硒标准，分别为0.17 mg·kg⁻¹（A）、0.22 mg·kg⁻¹（B）和0.24 mg·kg⁻¹（C），且C处理显著高于A、B处理（P＜0.05）；（2）不同生育期，水稻各器官中硒含量及累积量不同，各器官累积量均表现为：C处理＞B处理＞A处理。水稻硒的生物富集高峰期在抽穗期，成熟期水稻各器官硒的累积量表现为精米＞茎＞叶＞根＞颖壳；（3）整个生育时期硒有一定的运转规律：苗期到抽穗期，硒由根向茎、叶中转移，抽穗期到成熟期穗中硒含量主要来自于茎叶的供给。（4）根系硒的吸收系数和初级转运系数随着盐碱程度加重而增加，表现为C处理＞B处理＞A处理，而次级转移系数随着盐碱程度加重而降低，表现为A处理＞B处理＞C处理。结论在pH为8.41～9.51、全盐含量为1.74～3.62 g·kg⁻¹、全硒含量为0.23 mg·kg⁻¹的盐碱富硒土壤上能生产出符合富硒标准水稻，且中重度盐碱土壤能促进水稻对硒的吸收转运及积累，为盐碱富硒土壤开发富硒水稻提供理论支撑和依据。
- 盐碱富硒土壤 /
- 水稻 /
- 硒元素 /
- 吸收转运积累
Abstract: Objective Effects of different types of saline-alkali soil that are selenium (Se)-rich on the absorption, transport, and accumulation of Se in rice grown on them were studied. Method Saline-alkali-tolerant Ningjing 45 seedlings were grown on soils in the Yellow River irrigation area of Ningxia province that were mildly saline (Treatment A), mildly alkaline (Treatment B), and moderate-severely alkaline (Treatment C). Se uptake, accumulation, and quality of the seedlings grown on the pots were determined. Result (1) The Se contents in the roots, stems, leaves, and grains of the rice plants ranked as Treatment C＞Treatment B＞Treatment A, and at maturity, roots＞stems＞leaves＞grains. The contents in mature grains were 0.17 mg·kg⁻¹ under Treatment A, 0.217 mg·kg⁻¹ under Treatment B, and 0.24 mg·kg⁻¹ under Treatment C. They all met the enrichment standard set for rice; however, Treatment C produced a significantly higher level of the metal element in the plants than the other two treatments (P＜0.05). (2) The Se accumulation in rice peaked at heading stage but varied in the organs at maturity with the ranking of milled rice＞stems＞leaves＞roots＞glume. (3) In seedling to heading stage, Se transported basically from the roots to the stems and leaves, but in heading to maturity, mainly from the stems and leaves to the spikes. (4) The Se absorption and primary transport coefficients of the roots increased with increasing salinity and alkalinity in soil. Thus, the effect was the greatest under Treatment C, followed by Treatment B and Treatment A. In contrast, the secondary transfer coefficient decreased with the increases to reverse the ranking order. Conclusion It appeared that Se-enrichment for rice grown on a saline-alkali soil that had an ample supply of the metal element could be realized if the soil had a pH ranging between 8.41 and 9.51, salt content between 1.74 g·kg⁻¹ and 3.62 g·kg⁻¹, and Se content of 0.23 mg·kg⁻¹. And, interestingly, moderate and heavy saline-alkali soils bolstered the absorption, transport, and accumulation of Se in rice, and therefore, the normally considered undesirable conditions would enhance, rather than hinder, the use of like land masses.
- Selenium-rich saline-alkali soil /
- rice /
- selenium /
- absorption, transport, and accumulation

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图 1 盐碱富硒条件下水稻不同生育期各器官硒含量变化

不同小写字母表示不同处理间存在显著差异（P＜0.05）。A、B、C分别表示轻度盐化土壤、轻度碱化土壤、中重度碱化土壤，下同。

Figure 1. Se contents in organs of rice at growth stages in selenium-rich saline-alkali soil

Datas with different lowercase letters indicate significant differences at ＜0.05. A, B, and C represent mildly saline soil, mildly alkaline soil, and moderate-severely alkaline soil, respectively. Same for below.

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图 2 盐碱富硒条件下水稻不同生育期各器官硒积累量变化

Figure 2. Se accumulation in organs of rice at growth stages in selenium-rich saline-alkali soil

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图 3 盐碱富硒条件下水稻全株硒累积量在整个生育期的变化

Figure 3. Se accumulation of rice plant during growth in selenium-rich saline-alkali soil

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图 4 不同盐碱富硒条件下水稻硒累积量分布动态变化

Figure 4. Dynamic changes of Se accumulation in rice in selenium-rich saline-alkali soil

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表 1 参试土壤基本性状

Table 1. Basic properties of soils under study

处理 Treatment	土壤类型 Soil types	土层 Depth/cm	酸碱度 pH	全盐含量 Total salt matter/ （g·kg⁻¹）	有机质含量 Organic/ （g·kg⁻¹）	全氮含量 Total N/ （g·kg⁻¹）	有效磷含量 Available P/ （mg·kg⁻¹）	速效钾含量 Available K/ （mg·kg⁻¹）
A	轻度盐化土壤	0~20	8.41±0.06 c	2.68±0.38 b	10.01±0.39 c	0.68±0.01 a	23.21±0.42 c	114.03±1.02 c
B	轻度碱化土壤	0~20	8.80±0.03 b	1.74±0.03 c	12.14±0.13 b	0.52±0.01 c	23.85±0.32 b	162.86±0.84 a
C	中重度碱化土壤	0~20	9.51±0.11 a	3.62±0.13 a	12.29±0.23 a	0.58±0.01 b	24.41±0.16 a	160.56±1.22 b
同列数据后不同字母表示不同处理间存在显著差异（P＜0.05）。 Datas with different letters on same column indicate significant differences at P＜0.05.

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表 2 供试土壤硒含量

Table 2. Selenium content of soil to be tested

试验地 Test site	土层 Depth/cm	土壤全硒含量 selenium content / （mg·kg⁻¹）	土壤有效硒含量 Available selenium content （μg·kg⁻¹）
A	0-20	0.23±0.11a	19.58±3.52b
B	0-20	0.23±0.16a	25.25±5.79ab
C	0-20	0.23±0.14a	31.99±7.06a

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表 3 不同盐碱类型下水稻的吸收系数与转移系数

Table 3. Absorption and transfer coefficients of rice grown in soils of different types of salinity and alkalinity

处理 Treatment	根系吸收系数 RAI	初级转运系数 PTI	次级转运系数 STI
A	3.66 c	0.37 c	0.59 a
B	5.47 b	0.41 b	0.47 b
C	5.97 a	0.44 a	0.41 c

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参考文献(37)

[1]	王佳丽, 黄贤金, 钟太洋, 等. 盐碱地可持续利用研究综述 [J]. 地理学报, 2011, 66(5):673−684. WANG J L, HUANG X J, ZHONG T Y, et al. Review on sustainable utilization of salt-affected land [J]. Acta Geographica Sinica, 2011, 66(5): 673−684.(in Chinese)
[2]	NEWMAN R, WATERLAND N, MOON Y, et al. Selenium biofortification of agricultural crops and effects on plant nutrients and bioactive compounds important for human health and disease prevention - a review [J]. Plant Foods for Human Nutrition, 2019, 74(4): 449−460. doi: 10.1007/s11130-019-00769-z
[3]	NADERI M, PUAR P, ZONOUZI-MARAND M, et al. A comprehensive review on the neuropathophysiology of selenium [J]. The Science of the Total Environment, 2021, 767: 144329. doi: 10.1016/j.scitotenv.2020.144329
[4]	董起广, 何振嘉, 高红贝, 等. 沿黄地区盐碱地种植水稻土壤理化性质的比较 [J]. 植物资源与环境学报, 2017, 26(2):110−112. DONG Q G, HE Z J, GAO H B, et al. Comparison on soil physicochemical properties of saline and alkaline land planted with Oryza sativa in the area along the Yellow River [J]. Journal of Plant Resources and Environment, 2017, 26(2): 110−112.(in Chinese)
[5]	宁夏回族自治区国土资源调查监测院. 宁夏富硒土壤标准[M]. 银川: 宁夏人民教育出版社, 2018.
[6]	王志强, 杨建锋, 魏丽馨, 等. 石嘴山地区碱性土壤硒地球化学特征及生物有效性 [J]. 物探与化探, 2022, 46(1):229−237. WANG Z Q, YANG J F, WEI L X, et al. Geochemical characteristics and bioavailability of selenium in alkaline soil in Shizuishan area, Ningxia [J]. Geophysical and Geochemical Exploration, 2022, 46(1): 229−237.(in Chinese)
[7]	陈继平, 任蕊, 王晖, 等. 关中塿土地区土壤pH变化对硒形态及有效性的影响 [J]. 西北地质, 2020, 53(1):254−260. CHEN J P, REN R, WANG H, et al. Effect of Lou soil pH change on selenium forms and availability [J]. Northwestern Geology, 2020, 53(1): 254−260.(in Chinese)
[8]	李伟, 李飞, 毕德, 等. 兰州碱性土壤与农产品中硒分布及形态研究 [J]. 土壤, 2012, 44(4):632−638. LI W, LI F, BI D, et al. Spatial distribution and speciation of selenium in alkaline soils and agricultural products of Lanzhou [J]. Soils, 2012, 44(4): 632−638.(in Chinese)
[9]	WINKEL L H E, JOHNSON C A, LENZ M, et al. Environmental selenium research: From microscopic processes to global understanding [J]. Environmental Science & Technology, 2012, 46(2): 571−579.
[10]	WANG D, ZHOU F, YANG W X, et al. Selenate redistribution during aging in different Chinese soils and the dominant influential factors [J]. Chemosphere, 2017, 182: 284−292. doi: 10.1016/j.chemosphere.2017.05.014
[11]	杨奎. 安徽省庐江县潜在富硒土壤硒生物有效性研究[D]. 合肥: 合肥工业大学, 2018. YANG K. Study on selenium bio-availability of soil in potentially selenium enriched soils in Lujiang County, Anhui Province[D]. Hefei: Hefei University of Technology, 2018. (in Chinese)
[12]	马迅, 诸旭东, 宗良纲, 等. 不同调控措施对酸性富硒土壤硒有效性及水稻产量性状的影响 [J]. 土壤, 2018, 50(2):284−290. MA X, ZHU X D, ZONG L G, et al. Effects of mixed application of calcium magnesium phosphate and aminoacid foliar fertilizer on Se availability in acid Se-rich soil and on trait of rice yield [J]. Soils, 2018, 50(2): 284−290.(in Chinese)
[13]	诸旭东. 酸性富硒土壤中硒有效性的内源调控技术及其应用效果研究[D]. 南京: 南京农业大学, 2016. ZHU X D. Application and effectiveness of endogenous regulation in acidic Se-rich soil[D]. Nanjing: Nanjing Agricultural University, 2016. (in Chinese)
[14]	陈雪, 沈方科, 梁欢婷, 等. 外源施硒措施对水稻产量品质及植株硒分布的影响 [J]. 南方农业学报, 2017, 48(1):46−50. CHEN X, SHEN F K, LIANG H T, et al. Effects of exogenous selenium application on rice yield, quality, distribution of selenium in seedling [J]. Journal of Southern Agriculture, 2017, 48(1): 46−50.(in Chinese)
[15]	晏娟, 朱同贵, 张忠平, 等. 不同供硒水平对水稻硒的吸收和累积特性研究 [J]. 土壤, 2021, 53(2):272−276. YAN J, ZHU T G, ZHANG Z P, et al. Effect of Se supplying levels on Se absorption and accumulation in rice [J]. Soils, 2021, 53(2): 272−276.(in Chinese)
[16]	李玉梅, 王根林, 李艳, 等. 水稻对有机态硒的吸收与积累 [J]. 中国农学通报, 2017, 33(10):7−11. LI Y M, WANG G L, LI Y, et al. Organic selenium absorption and accumulation of rice [J]. Chinese Agricultural Science Bulletin, 2017, 33(10): 7−11.(in Chinese)
[17]	李建东, 郑慧莹. 松嫩平原盐碱化草地治理及其生物生态机理[M]. 北京: 科学出版社, 1997.
[18]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
[19]	中华人民共和国农业部. 土壤中全硒的测定: NY/T 1104—2006[S]. 北京: 中国标准出版社, 2006.
[20]	中华人民共和国农业部. 土壤中有效硒的测定氰化物发生原子荧光光谱法: NY/T 3420—2019[S]. 北京: 中国标准出版社, 2019
[21]	周鑫斌, 赖凡, 张城铭, 等. 不同形态硒向水稻籽粒转运途径及品种差异[J]. 土壤学报, 2017, 54(5): 1251－1258. ZHOU X B, LAI F, ZHANG C M, et al. Pathways of selenium to grain relative to form of selenium and variety of rice(Oryza sativa L. )[J]. Acta Pedologica Sinica, 2017, 54(5): 1251－1258. (in Chinese)
[22]	王瑞昕. 磷素营养和水分管理对水稻吸收积累硒的影响[D]. 重庆: 西南大学, 2021. WANG R X. The effect of phosphorus nutrition and water management on the absorption and accumulation of selenium in rice[D]. Chongqing: Southwest University, 2021. (in Chinese)
[23]	ZAWISLANSKI P T, ZAVARIN M. Nature and rates of selenium transformations: A laboratory study of kesterson reservoir soils [J]. Soil Science Society of America Journal, 1996, 60(3): 791−800. doi: 10.2136/sssaj1996.03615995006000030016x
[24]	LI J, PENG Q, LIANG D L, et al. Effects of aging on the fraction distribution and bioavailability of selenium in three different soils [J]. Chemosphere, 2016, 144: 2351−2359. doi: 10.1016/j.chemosphere.2015.11.011
[25]	赵妍, 宗良纲, 曹丹, 等. 江苏省典型茶园土壤硒分布特性及其有效性研究 [J]. 农业环境科学学报, 2011, 30(12):2467−2474. ZHAO Y, ZONG L G, CAO D, et al. Distribution and availability of selenium in typical tea garden of Jiangsu Province, China [J]. Journal of Agro-Environment Science, 2011, 30(12): 2467−2474.(in Chinese)
[26]	安梦鱼. 富硒土壤硒形态转化的主要影响因子及对作物吸收的研究[D]. 福州: 福建农林大学, 2017 AN M Y. The main influencing factors of selenium transformation in Se-rich soil and its effects on plant absorption[D]. Fuzhou: Fujian Agriculture and Forestry University, 2017. (in Chinese)
[27]	SINGH M, SINGH N, RELAN P S. Adsorption and desorption of selenite and selenate selenium on different soils [J]. Soil Science, 1981, 132(2): 134−141. doi: 10.1097/00010694-198108000-00002
[28]	周鑫斌, 施卫明, 杨林章. 叶面喷硒对水稻籽粒硒富集及分布的影响 [J]. 土壤学报, 2007, 44(1):73−78. ZHOU X B, SHI W M, YANG L Z. Effect of foliar application of selenite on selenium accumulation and distribution in rice [J]. Acta Pedologica Sinica, 2007, 44(1): 73−78.(in Chinese)
[29]	周鑫斌, 施卫明, 杨林章. 富硒与非富硒水稻品种对硒的吸收分配的差异及机理 [J]. 土壤, 2007, 39(5):731−736. ZHOU X B, SHI W M, YANG L Z. Genotypical differences and characteristics of Se uptake and accumulation in rice [J]. Soils, 2007, 39(5): 731−736.(in Chinese)
[30]	靳元元, 马生军, 徐万里, 等. 不同产地黄芪活性成分与硒含量比较分析 [J]. 新疆农业科学, 2021, 58(5):873−881. JIN Y Y, MA S J, XU W L, et al. Comparative analysis of active ingredients and selenium contents in Astragalus from different habitats [J]. Xinjiang Agricultural Sciences, 2021, 58(5): 873−881.(in Chinese)
[31]	CHEN Y P, PENG Q, LIANG D L, et al. Effects of nitrogen application on selenium uptake, translocation and distribution in winter wheat [J]. Huan Jing Ke Xue= Huanjing Kexue, 2017, 38(2): 825−831.
[32]	付冬冬. 不同外源硒对冬小麦硒吸收、分配和转运的影响[D]. 杨凌: 西北农林科技大学, 2011. FU D D. Effects of different concentration of exogenetic selenite and selenate on selenium uptake, distribution and translocation of winter wheat[D]. Yangling: Northwest A & F University, 2011. (in Chinese)
[33]	马强, 姬丙艳, 张亚峰, 等. 青海东部土壤及生物体中硒的地球化学特征 [J]. 地球科学进展, 2012, 27(10):1148−1152. MA Q, JI B Y, ZHANG Y F, et al. Geochemical characteristics of selenium in soils and organisms in the east of Qinghai Province [J]. Advances in Earth Science, 2012, 27(10): 1148−1152.(in Chinese)
[34]	黄青青, 杜威, 王琪, 等. 水稻对不同土壤中硒酸盐/亚硒酸盐的吸收和富集 [J]. 环境科学学报, 2013, 33(5):1423−1429. HUANG Q Q, DU W, WANG Q, et al. Uptake and accumulation of Se by rice in different soils supplied with selenite or selenate [J]. Acta Scientiae Circumstantiae, 2013, 33(5): 1423−1429.(in Chinese)
[35]	张联合, 李友军, 苗艳芳, 等. pH对水稻离体根系吸收亚硒酸盐生理机制的影响 [J]. 土壤学报, 2010, 47(3):523−528. ZHANG L H, LI Y J, MIAO Y F, et al. Effects of pH on the physiological mechanism of selenite uptake by excised roots of rice seedlings [J]. Acta Pedologica Sinica, 2010, 47(3): 523−528.(in Chinese)
[36]	罗杰, 王佳媛, 游远航, 等. 硒在土壤—水稻系统中的迁移转化规律 [J]. 西南师范大学学报(自然科学版), 2012, 37(3):60−66. LUO J, WANG J Y, YOU Y H, et al. Migration and transformation of Se in the soil-rice system [J]. Journal of Southwest China Normal University (Natural Science Edition), 2012, 37(3): 60−66.(in Chinese)
[37]	姜超强, 沈嘉, 祖朝龙. 水稻对天然富硒土壤硒的吸收及转运 [J]. 应用生态学报, 2015, 26(3):809−816. JIANG C Q, SHEN J, ZU C L. Selenium uptake and transport of rice under different Se-enriched natural soils [J]. Chinese Journal of Applied Ecology, 2015, 26(3): 809−816.(in Chinese)