Mechanism of Red or Blue Light in Regulating Root Development of Tobacco Seedlings
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摘要:
目的 探究红蓝光质调控烟苗根系发育的机理。 方法 以烤烟品种翠碧1号为试材，采用水培试验，研究红蓝光对烟草幼苗根系生长、内源气体信号分子硫化氢（H2S）合成和积累的影响。 结果 处理7 d时，与对照白光处理相比，结果表明：（1）红光处理烟苗地上部和根系生物量显著增加，增幅分别为74.62%和15.64%，蓝光根系生物量降幅为7.00%；（2）红光处理烟苗根系H2S含量显著增加，增幅为61.72%，而蓝光处理显著降低；（3）红光处理烟苗根系脱巯基酶（DES）、半胱氨酸合成酶（CS）活性分别升高24.28%、25.61%；蓝光处理则分别降低32.10%、18.30%；（4）红光处理的H2S生物合成关键基因NtDES表达量为白光处理的8.8倍，蓝光处理仅为白光处理的13.82%。 结论 红光处理可通过增强H2S生物合成关键酶编码基因的表达提高烟苗根系H2S的含量，进而促进侧根的发生，而蓝光效应与红光相反。 Abstract:Objective Mechanism of red or blue light in regulating the root development of flue-cured tobacco seedlings was studied. Method Effects of red or blue LED light exposure on the root growth and the synthesis and accumulation of endogenous gas signal molecule hydrogen sulfide (H2S) in CB-1 tobacco seedlings were monitored. Results After 7 days of the exposure to red or blue LED in comparison to white light, (1) the biomasses of aboveground parts and roots of the seedlings treated by red light increased by 74.62% and 15.64%, respectively, while the roots of the seedlings exposed to the blue LED decreased by 7.00%; (2) the H2S content on the red light-treated plants increased by 61.72%, but reduced on the blue light-treated counterparts; (3) the red LED increased the activities of DES and CS by 24.28% and 25.61%, respectively, but the blue LED decreased them by 32.10% and 18.30%, respectively; (4) the NtDES expression in the red light-treated seedlings were 8.8-fold of that in the plants treated by white light, whereas the blue light resulted in merely 13.82% of what white light did. Conclusion In contrast to blue LED, the exposure of the tobacco seedlings to red LED rose H2S in the plants through increased expression of the key enzyme coding genes in the biosynthesis inducing a thriving generation of lateral roots. -
Key words:
- Red or blue LED light exposure /
- tobacco /
- lateral root /
- hydrogen sulfide
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图 2 不同光质对烟苗生长的影响
不同小写字母表示处理间差异有统计学意义（P＜0.05），下同。
Figure 2. Effects of different LEDs on growth of tobacco seedlings
Data with different lowercase letters indicate significant differences at P＜0.05. Same for below.
图 3 不同光质处理对烟苗根系中H2S含量的影响
Figure 3. Effects of different LEDs on H2S content in tobacco seedlings
图 4 不同光质处理对烟苗体内H2S合成关键酶活性的影响
Figure 4. Effects of different LEDs on activities of key enzymes in H2S synthesis of tobacco seedlings
图 5 不同光质处理对NtDES表达量的影响
Figure 5. Effects of different LEDs on NtDES expression in seedlings
图 6 H2S供体和清除剂对烟苗侧根发生的影响
Figure 6. Effects of H2S donor and scavenger on lateral root formation of tobacco seedlings
表 1 NtDES和NtL25引物序列
Table 1. Primers of NtDES and NtL25 for qRT-PCR
基因名称
Gene引物序列
Primer sequenceNtDES F: 5'-AAATAACTGGCAATGGGGTT-3'
R: 5'-TGGTCTGTTGGCGAGTCTAA-3'NtL25 F: 5'-AGTCTTAGGTCGGTGGA-3'
R: 5'-AGCTGGTAGATTTCTCGT-3'
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