基于ITS基因条码的保健食品中植物源性成分的鉴定

邱希斌

doi:10.19303/j.issn.1008-0384.2023.08.014

基于ITS基因条码的保健食品中植物源性成分的鉴定

doi: 10.19303/j.issn.1008-0384.2023.08.014

邱希斌

福建省产品质量检验研究院/国家加工食品质量检验检测中心（福州），福建　福州　350002

基金项目: 福建省市场监督管理局科技项目（FJMS2020002）；福建省产品质量检验研究院科技项目（KY202012A）

详细信息

作者简介:
邱希斌（1991 —），男，硕士，工程师，主要从事加工食品检验及分子生物学研究，E-mail：120734300@qq.com

中图分类号: Q751
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出版历程
- 收稿日期: 2022-09-15
- 修回日期: 2023-05-25
- 网络出版日期: 2023-08-16
- 刊出日期: 2023-08-28

Utilization of ITS Gene Barcodes for Identifying Plant-derived Functional Ingredients in Health Food Products

QIU Xibin

Fujian Inspection and Research Institute for Product Quality/National Quality Supervision and Testing Center for Processed Food (FuZhou) , Fuzhou, Fujian　350002, China

摘要

摘要: 目的研究并建立运用ITS基因条码技术针对保健食品常见植物原料源性成分的分子生物学鉴定技术。方法通过采用回顾性调查方法，选取西洋参（Panax quinquefolius）、铁皮石斛（Dendrobium candidum）以及红景天（Rhodiola nobilis）、枸杞子（Lycium barbarum L.）与人参Ginseng（Radix Ginseng）作为研究对象。探索其作为生产原料的常见保健食品剂型中，目标 DNA 的最佳提取方法。运用ITS2内转录间隔区保守特性设计引物序列，并通过调整优化体系中各物质的组分，由此构建稳定高效准确的PCR反应体系。结果采用改良CTAB法可实现对所研究的保健食品中常见剂型目标DNA的提取，纯度及质量达到反应要求。所建立的PCR法扩增效果良好，对西洋参、铁皮石斛以及红景天的方法灵敏度均为1 ng·μL⁻¹，枸杞子与人参的方法灵敏度为0.1 ng·μL⁻¹。结论运用建立的方法可实现对保健食品中常见植物性原料西洋参、人参、铁皮石斛、枸杞子、红景天成分的鉴定，适用于丸剂、片剂、口服液等不同剂型的保健食品中原料鉴别，可运用于对保健食品中植物源性的检测。
- 基因条码技术 /
- 保健食品 /
- 植物源性成分 /
- PCR
Abstract: Objective A molecular biology identification method to detect common plant-derived functional ingredients in health food products using genetic barcodes was developed. Method By means of retrospective investigation, 5 common health food raw materials in China, i.e., American ginseng (Panax quinquefolius), Dendrobium candidum, rhodiola (Rhodiola nobilis), wolfberries (Lycium barbarum L.), and ginseng (Radix Ginseng), were firstly tested for functional ingredients using the best extraction method on the target DNA. A stable, efficient, and accurate PCR reaction system was constructed with primer sequence designed based on the conservative characteristics of ITS2 internal transcribed spacer and components of each substance adjusted and optimized. Molecular identification on health food products of various forms was tested using the developed method. Result The innovative application of a modified CTAB method afforded successful extraction of the target DNA in health food materials as well as products at commonly formulated dosages. The PCR amplified with the detection sensitivity on nucleic acids of American ginseng, D. candidum, and rhodiola reached 1 ng·μL⁻¹, and of wolfberries and ginseng 0.1 ng·μL⁻¹. Conclusion By using the ITS gene barcodes obtained through the DNA extraction and PCR amplification, the key functional ingredients from plant materials such as American ginseng, ginseng, D. candidum, wolfberries, and rhodiola rosea could be identified for authentication of health food products in the forms of pills, tablets, and concoctions.
- Gene barcode /
- health food /
- plant-derived functional ingredients /
- PCR

HTML全文

图 1 ITS2基因特异片段PCR产物电泳图

M: DNA Marker;CK:空白；N:阴性对照；A图1—10：西洋参药材；B图1-10：人参药材；C图1—10：枸杞药材；D图1-10：红景天药材；E图1-10：铁皮石斛。

Figure 1. Electrophoresis on PCR products of ITS2 specific fragment

M: DNA marker; CK: blank; N: negative control; Fig. A 1 — 10: American ginseng; Fig. B 1 — 10: ginseng; Fig. C 1 — 10: Chinese wolfberry; Fig. D 1 — 10: rhodiola; Fig. E 1 — 10: D. candidum.

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图 2 灵敏度试验结果

M: DNA Marker；泳道1 — 8为西洋参DNA质量浓度，依次为：0.01、0.1、1 ng·μL⁻¹；泳道9 — 16为铁皮石斛DNA质量浓度，依次为：0.01、0.1、1 ng·μL⁻¹；泳道17 — 24为红景天DNA质量浓度，依次为：0.01、0.1、1 ng·μL⁻¹; 泳道25 — 30为枸杞子DNA质量浓度，依次为：0.01、0.1、1 ng·μL⁻¹；泳道31 — 36为人参DNA质量浓度，依次为：0.01、0.1、1 ng·μL⁻¹。

Figure 2. Sensitivity of assay

M: DNA marker; Swimlane No. 1-8: American ginseng DNA mass concentrations at 0.01, 0.1, and 1 ng·μL⁻¹; Swimlane No. 9-16: D. candidum DNA mass concentrations at 0.01, 0.1, and 1 ng·μL⁻¹; Swimlane No. 17-24: rhodiola DNA mass concentrations at 0.01, 0.1, and 1 ng·μL⁻¹; Swimlane No. 25-30: Chinese wolfberry DNA mass concentrations at 0.01, 0.1, and 1 ng·μL⁻¹; Swimlane No. 9-16: ginseng DNA mass concentrations at 0.01, 0.1, and 1 ng·μL⁻¹.

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图 3 序列BLAST比对图

A，人参；B，西洋参；C，枸杞子；D，铁皮石斛；E，红景天。

Figure 3. BLAST comparison on sequences

A: ginseng; B: American ginseng; C: Chinese wolfberry; D: D. candidum; E: rhodiola.

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图 4 ITS 2 DNA条形码植物鉴别NJ系统发育树

Figure 4. Neighbor joining tree of health plant materials constructed using ITS 2 DNA barcodes

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图 5 部分供试保健食品样品ITS2基因PCR产物电泳图

M: DNA Marker; CK：空白对照；N：阴性对照；P：阳性对照；A1 — 12：西洋参供试样品；B1 — 8：人参供试样品；C1 — 10：铁皮石斛供试样品；D1 — 10：红景天供试样品。

Figure 5. Electrophoresis on PCR products of ITS2 genes in some health food products

M: DNA marker; CK: blank; N: negative control; A1-12：American ginseng samples; B1-8: ginseng samples; C1-10: D. candidum samples; D1-10: rhodiola samples.

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表 1 供试药材信息

Table 1. Information on test materials

序号 Serial No	物种名称及编号 Species name and number	产品状态 Product State	产地 Place of Origin
1	西洋参1 American ginseng 1	粉末Powder	云南昆明 Kunming, Yunnan
2	西洋参2 American ginseng 2	药材 Medicinal materials	黑龙江饶河 Raohe, Heilongjiang
3	西洋参3 American ginseng 3	药材 Medicinal materials	加拿大 Canada
4	西洋参4 American ginseng 4	饮片 Decoction pieces	吉林清河 Qinghe,Jilin
5	西洋参5 American ginseng 5	饮片 Decoction pieces	美国 U.S.A
6	人参1 Ginseng1	鲜品 Fresh products	吉林通化 Tonghua ,Jilin
7	人参2 Ginseng2	鲜品 Fresh products	吉林延吉 Yanji ,Jilin
8	人参3 Ginseng3	鲜品 Fresh products	吉林长白山 Changbai Mountain, Jilin
9	人参4 Ginseng4	鲜品 Fresh products	黑龙江饶河 Raohe, Heilongjiang
10	人参5 Ginseng5	饮片 Decoction pieces	新西兰 New Zealand
11	枸杞子1 Chinese wolfberry1	药材 Medicinal materials	内蒙古 Inner Mongolia
12	枸杞子2 Chinese wolfberry2	药材 Medicinal materials	宁夏 Ningxia
13	枸杞子3 Chinese wolfberry3	药材 Medicinal materials	甘肃陇南 Longnan, Gansu
14	枸杞子4 Chinese wolfberry4	药材 Medicinal materials	山东日照 Rizhao, Shandong
15	枸杞子5 Chinese wolfberry5	药材 Medicinal materials	河北邢台 Xingtai, Hebei
16	铁皮石斛1 Dendrobium candidum1	粉末 Powder	湖南邵阳 Shaoyang, Hunan
17	铁皮石斛2 Dendrobium candidum2	药材 Medicinal materials	浙江天台 Tiantai, Zhejiang
18	铁皮石斛3 Dendrobium candidum3	药材 Medicinal materials	福建宁化 Ninghua, Fujian
19	铁皮石斛4 Dendrobium candidum4	饮片 Decoction pieces	广西天峨 Tian'e, Guangxi
20	铁皮石斛5 Dendrobium candidum5	饮片 Decoction pieces	云南文山 Wenshan, Yunnan
21	红景天1 Rhodiola1	饮片 Decoction pieces	内蒙古兴安 Xing'an, Inner Mongolia
22	红景天2 Rhodiola2	药材 Medicinal materials	吉林延吉 Yanji ,Jilin
23	红景天3 Rhodiola3	药材 Medicinal materials	西藏拉萨 Tibet Lhasa
24	红景天4 Rhodiola4	饮片 Decoction pieces	黑龙江饶河 Raohe, Heilongjiang
25	红景天5 Rhodiola5	饮片 Decoction pieces	甘肃兰州 Lanzhou, Gansu

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表 2 供试保健食品信息

Table 2. Information on health food products

序号 Serial No	保健食品名称 Name of health food	剂型 Dosage form	配料表宣称所含目标植物 Target plants claimed in the ingredient list
1	西洋参含片 American ginseng buccal tablet	片剂 Tablet	西洋参 American ginseng
2	美国洋参含片 American ginseng buccal tablet	片剂 Tablet	西洋参 American ginseng
3	西洋参三七胶囊 Panax quinquefolium and panax notoginseng capsules	胶囊 Capsule	西洋参 American ginseng
4	西洋参淫羊藿枸杞子软胶囊 Panax quinquefolium epimedium and wolfberry fruit soft capsules	胶囊 Capsule	西洋参 American ginseng
5	西洋参三七口服液 American ginseng and panax notoginseng oral liquid	口服液 oral liquid	西洋参 American ginseng
6	灵芝西洋参口服液 Ganoderma lucidum and American ginseng oral liquid	口服液 oral liquid	西洋参 American ginseng
7	人参提取口服液 Ginseng Extract Oral Liquid	口服液 oral liquid	人参 Ginseng
8	人参氨基酸口服液 Ginseng Amino Acid Oral Liquid	口服液 oral liquid	人参 Ginseng
9	人参三七含片 Ginseng and Sanqi Buccal Tablets	片剂 Tablet	人参 Ginseng
10	人参滴丸 Ginseng dropping pill	丸剂 Pills	人参 Ginseng
11	杞苓枣氨基酸口服液 Jujube Amino Acid Oral Liquid	口服液 oral liquid	枸杞子 Chinese wolfberry
12	玛咖枸杞胶囊 Maca medlar capsule	胶囊 Capsule	枸杞子 Chinese wolfberry
13	氨基酸枸杞片 Amino acid medlar tablet	片剂 Tablet	枸杞子 Chinese wolfberry
14	阿胶枸杞颗粒冲剂 Donkey hide gelatin and wolfberry granules	颗粒剂 Granule	枸杞子 Chinese wolfberry
15	森山铁皮枫斗颗粒 Senshan Iron Sheet Granules	冲剂 Granules	铁皮石斛 Dendrobium candidum
16	九仙尊米斛枫斗浆 Nine Immortals Zun Rice Dendrobium Maple Juice	片剂 Tablet	铁皮石斛 Dendrobium candidum
17	铁皮枫斗口服液 Dendrobium candidum oral liquid	口服液 oral liquid	铁皮石斛 Dendrobium candidum
18	津玉石斛口服液 Jinyu Dendrobium Oral Liquid	口服液 oral liquid	铁皮石斛 Dendrobium candidum
19	铁皮石斛西洋参黄芪胶囊 Dendrobium candidum, American ginseng and astragalus capsule	胶囊 Capsule	铁皮石斛 Dendrobium candidum
20	红景天软胶囊 Rhodiola Soft Capsule	胶囊 Capsule	红景天 Rhodiola
21	红景天西洋参口服液 Rhodiola and American Ginseng Oral Liquid	口服液 oral liquid	红景天 Rhodiola
22	红景天提取物口服液 Rhodiola extract oral liquid	口服液 oral liquid	红景天 Rhodiola
23	红景天胶囊 Rhodiola Capsule	胶囊 Capsule	红景天 Rhodiola
24	圣莲红景天丸 Shenglian Rhodiola Capsule	丸剂 Pills	红景天 Rhodiola

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表 3 引物序列

Table 3. Primer sequences

片段 Fragment	引物名称 Primer name	引物序列（5'-3'） Primer sequence　（5'-3'）
ITS2	ITS2-F	ATGCGATACTTGGTGTGAAT
ITS2	ITS2-R	GACGCTTCTCCAGACTACAAT
18S	18S-F	CCTGAGAAACGGCTACCAT
	18S-R	CGTGTCAGGATTGGGTAAT

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表 4 不同方法DNA提取效果比较

Table 4. DNA extraction by different methods

物种 Species	传统碱裂解SDS法 Traditional alkali cracking method		改良CTAB法 Modified CTAB method		试剂盒法 Kit method
物种 Species	纯度 Purity A₂₆₀/A₂₈₀	得率 Yield/（ng·μL^-1）	纯度 Purity A₂₆₀/A₂₈₀	得率 Yield /（ng·μL⁻¹）	纯度 Purity A₂₆₀/A₂₈₀	得率 Yield/（ng·μL⁻¹）
西洋参1 American ginseng1	1.35±0.04 c	363±8.19c	1.85±0.04a	814±4.16 a	1.50±0.06b	494±1.53 b
西洋参2 American ginseng 2	1.31±0.03 b	494±6.11 b	1.84±0.03 a	831±10.15 a	1.54±0.04 b	481±5.57 b
西洋参3 American ginseng 3	1.22±0.04 c	424±2.52 c	1.86±0.04 a	865±9.54 a	1.72±0.03b	454±6.56 b
西洋参4 American ginseng 4	1.33±0.04 c	322±5.57 c	2.06±0.10 a	868±3.51 a	1.59±0.03 b	478±6.03 b
西洋参5 American ginseng 5	1.43±0.05 c	422±4.16c	1.94±0.04 a	787±2.65a	1.58±0.23 b	489±8.39 b
人参1 Ginseng1	1.37±0.03 c	315±3.06 c	1.85±0.05a	896±1.00 a	1.45±0.05 b	500±5.86 b
人参2 Ginseng2	1.34±0.04 c	321±9.24 c	1.86±0.04 a	807±2.08 a	1.52±0.02 b	504±1.53 b
人参3 Ginseng3	1.21±0.02 c	322±4.36 c	2.05±0.04 a	853±7.77 a	1.45±0.05 b	481±7.02 b
人参4 Ginseng4	1.32±0.03 c	378±3.79 c	1.84±0.04 a	803±3.51 a	1.46±0.05 b	484±2.52 b
人参5 Ginseng5	1.35±0.04 c	348±5.57 c	1.91±0.06 a	807±5.13 a	1.58±0.06 b	489±2.52 b
枸杞子1 Chinese wolfberry1	1.63±0.03 c	573±4.04 c	2.04±0.05 a	806±2.00 a	1.41±0.04 b	621±9.62 b
枸杞子2 Chinese wolfberry2	1.74±0.02 b	540±6.35 c	1.88±0.03 a	759±4.73 a	1.67±0.05 b	601±1.73 b
枸杞子3 Chinese wolfberry3	1.59±0.06 b	565±0.58 c	1.85±0.03 a	780±5.86 a	1.43±0.05 b	594±1.53 b
枸杞子4 Chinese wolfberry4	1.64±0.03 c	589±3.46 c	1.84±0.04 a	794±3.06 a	1.47±0.06 b	613±9.45 b
枸杞子5 Chinese wolfberry1	1.73±0.03 c	582±4.16 c	1.95±0.04 a	774±2.08 a	1.54±0.04 b	606±5.51 b
铁皮石斛1 Dendrobium candidum1	1.55±0.03 c	362±10.44 c	1.99±0.04 a	827±2.31 a	1.68±0.05 b	499±9.02 b
铁皮石斛2 Dendrobium candidum2	1.52±0.02 c	486±2.08 c	1.86±0.05 a	815±5.51 a	1.66±0.05 b	481±3.46 b
铁皮石斛3 Dendrobium candidum3	1.33±0.03 c	424±5.13 c	1.95±0.04 a	870±3.79 a	1.71±0.07 b	457±5.03 b
铁皮石斛4 Dendrobium candidum4	1.34±0.03 c	320±6.66 c	1.94±0.04 a	858±7.81 a	1.71±0.06 b	479±10.01 b
铁皮石斛5 Dendrobium candidum5	1.47±0.05 c	429±6.51 c	1.90±0.05 a	793±4.73 a	1.64±0.04 b	504±3.51 b
红景天1 Rhodiola1	1.40±0.06 b	382±9.87 c	1.88±0.04 a	886±2.89 a	1.46±0.02 b	512±1.73 b
红景天2 Rhodiola2	1.28±0.07 c	329±5.57 c	1.90±0.06 a	807±5.03 a	1.50±0.07 b	509±4.51 b
红景天3 Rhodiola3	1.25±0.04 c	330±2.52 c	1.98±0.03 a	846±6.08 a	1.62±0.05 b	486±4.04 b
红景天4 Rhodiola4	1.35±0.04 c	383±3.51 c	1.86±0.03 a	800±8.33 a	1.58±0.08 b	490±4.16 b
红景天5 Rhodiola5	1.34±0.04 c	348±8.50 c	1.94±0.04 a	810±5.77 a	1.63±0.06 b	488±8.39 b
同指标数据后小写字母不同表示 0.05 水平上差异显著。 Those with different lowercase letters on same indicator indicate significant difference at 0.05 level.

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表 5 供试保健食品基因组DNA的纯度与得率

Table 5. Purity and yield of genomic DNA of health food materials

保健食品编号 Health food No	保健食品名称 Name of health food	配料表宣称所含目标植物 Target plants claimed in the ingredient list	纯度 Purity A₂₆₀/A₂₈₀	DNA质量浓度 DNA concentration/ （ng·μL⁻¹）
西洋参供试样1 American ginseng samples 1	西洋参含片 American ginseng buccal tablet	西洋参 American ginseng	1.82	561
西洋参供试样2 American ginseng samples 2	美国洋参含片 American ginseng buccal tablet	西洋参 American ginseng	1.92	497
西洋参供试样3 American ginseng samples 3	西洋参三七胶囊 Panax quinquefolium and panax notoginseng capsules	西洋参 American ginseng	1.54	269
西洋参供试样4 American ginseng samples 4	西洋参淫羊藿枸杞子软胶囊 Panax quinquefolium epimedium and wolfberry fruit soft capsules	西洋参 American ginseng	1.55	341
西洋参供试样5 American ginseng samples 5	西洋参三七口服液 American ginseng and panax notoginseng oral liquid	西洋参 American ginseng	1.37	206
西洋参供试样6 American ginseng samples 1	灵芝西洋参口服液 Ganoderma lucidum and American ginseng oral liquid	西洋参 American ginseng	1.33	219
人参供试样1 Ginseng sample 1	人参提取口服液 Ginseng Extract Oral Liquid	人参 Ginseng	1.37	252
人参供试样2 Ginseng sample 2	人参氨基酸口服液 Ginseng Amino Acid Oral Liquid	人参 Ginseng	1.39	200
人参供试样3 Ginseng sample 3	人参三七含片 Ginseng and Sanqi Buccal Tablets	人参 Ginseng	1.76	578
人参供试样4 Ginseng sample4	人参滴丸 Ginseng dropping pill	人参 Ginseng	1.80	622
枸杞子供试样1 Chinese wolfberry sample 1	杞苓枣氨基酸口服液 Jujube Amino Acid Oral Liquid	枸杞子 Chinese wolfberry	1.52	213
枸杞子供试样2 Chinese wolfberry sample 2	玛咖枸杞胶囊 Maca medlar capsule	枸杞子 Chinese wolfberry	1.56	456
枸杞子供试样3 Chinese wolfberry sample 3	氨基酸枸杞片 Amino acid medlar tablet	枸杞子 Chinese wolfberry	1.90	389
枸杞子供试样4 Chinese wolfberry sample 4	阿胶枸杞颗粒冲剂 Donkey hide gelatin and wolfberry granules	枸杞子 Chinese wolfberry	1.79	420
铁皮石斛供试样1 Dendrobium candidum sample1	森山铁皮枫斗颗粒 Senshan Iron Sheet Granules	铁皮石斛 Dendrobium candidum	1.84	565
铁皮石斛供试样2 Dendrobium candidum sample2	九仙尊米斛枫斗浆 Nine Immortals Zun Rice Dendrobium Maple Juice	铁皮石斛 Dendrobium candidum	1.97	500
铁皮石斛供试样3 Dendrobium candidum sample3	铁皮枫斗口服液 Dendrobium candidum oral liquid	铁皮石斛 Dendrobium candidum	1.66	270
铁皮石斛供试样4 Dendrobium candidum sample4	津玉石斛口服液 Jinyu Dendrobium Oral Liquid	铁皮石斛 Dendrobium candidum	1.54	339
铁皮石斛供试样5 Dendrobium candidum sample5	铁皮石斛西洋参黄芪胶囊 Dendrobium candidum, American ginseng and astragalus capsule	铁皮石斛 Dendrobium candidum	1.36	205
红景天供试样1 Rhodiola sample1	红景天软胶囊 Rhodiola Soft Capsule	红景天 Rhodiola	1.38	220
红景天供试样2 Rhodiola sample2	红景天西洋参口服液 Rhodiola and American Ginseng Oral Liquid	红景天 Rhodiola	1.40	252
红景天供试样3 Rhodiola sample3	红景天提取物口服液 Rhodiola extract oral liquid	红景天 Rhodiola	1.53	403
红景天供试样4 Rhodiola sample4	红景天胶囊 Rhodiola Capsule	红景天 Rhodiola	1.77	580
红景天供试样5 Rhodiola sample5	圣莲红景天丸 Shenglian Rhodiola Capsule	红景天 Rhodiola	1.85	625

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表 6 部分供试保健食品源性检测对照表

Table 6. Origins of some health food products

保健食品编号 Health food No	保健食品名称 Name of health food	PCR产物电泳图及泳道编号 Electrophoresis diagram and Swimlane No of PCR products
西洋参供试样3 American ginseng samples 3	西洋参三七胶囊 Panax quinquefolium and panax notoginseng capsules	图5A，泳道编号5、6 Fig.5A，Swimlane No 5、6
西洋参供试样5 American ginseng samples 5	西洋参三七口服液 American ginseng and panax notoginseng oral liquid	图5A，泳道编号9、10 Fig.5A，Swimlane No 9、10
人参供试样1 Ginseng sample 1	人参提取口服液 Ginseng Extract Oral Liquid	图5B，泳道编号1、2 Fig.5B，Swimlane No 1、2
铁皮石斛供试样2 Dendrobium candidum sample2	九仙尊米斛枫斗浆 Nine Immortals Zun Rice Dendrobium Maple Juice	图5C，泳道编号3、4 Fig.5C，Swimlane No 3、4
铁皮石斛供试样3 Dendrobium candidum sample3	铁皮枫斗口服液 Dendrobium candidum oral liquid	图5C，泳道编号5、6 Fig.5C，Swimlane No 5、6
红景天供试样1 Rhodiola sample1	红景天软胶囊 Rhodiola Soft Capsule	图5D，泳道编号1、2 Fig.5D，Swimlane No 1、2
红景天供试样3 Rhodiola sample3	红景天提取物口服液 Rhodiola extract oral liquid	图5D，泳道编号5、6 Fig.5D，Swimlane No 5、6

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表 7 供试保健食品基因组测序比对结果

Table 7. Genome sequences of health food products

保健食品名称 Name of health food	配料表宣称所含目标植物 Target plants claimed in the ingredient list	GenBank数据库 GenBank Database			中药材 DNA条形码数据库 DNA Barcode Database of Chinese Medicinal Materials
保健食品名称 Name of health food	配料表宣称所含目标植物 Target plants claimed in the ingredient list	BLAST鉴定结果 BLAST identification results	相似度 Similarity/%	GenBank登录号 GenBank Login Number	最佳匹配 Best Fit	序列相似度 Sequence similarity/%	Score值 Score value
西洋参含片 American ginseng buccal tablet	西洋参 American ginseng	西洋参 American ginseng	99	HQ112440.1	西洋参 American ginseng	100.0	470.0
美国洋参含片 American ginseng buccal tablet	西洋参 American ginseng	西洋参 American ginseng	97	MG217889.1	西洋参 American ginseng	98.5	410.0
西洋参三七胶囊 Panax quinquefolium and panax notoginseng capsules	西洋参 American ginseng	党参 Codonopsis pilosula	98	EF190460	党参 Codonopsis pilosula	98	403.0
西洋参淫羊藿枸杞子软胶囊 Panax quinquefolium epimedium and wolfberry fruit soft capsules	西洋参 American ginseng	西洋参 American ginseng	97	KM036297.1	西洋参 American ginseng	99.1	460.0
西洋参三七口服液 American ginseng and panax notoginseng oral liquid	西洋参 American ginseng	生晒参 Raw sun dried ginseng	99	HQ112363.1	生晒参 Raw sun dried ginseng	97.6	425.0
灵芝西洋参口服液 Ganoderma lucidum and American ginseng oral liquid	西洋参 American ginseng	西洋参 American ginseng	99	MG217889.1	西洋参 American ginseng	100.0	440.0
人参提取口服液 Ginseng Extract Oral Liquid	人参 Ginseng	竹节参 Panax japonicus	98	KX674924.1	竹节参 Panax japonicus	99.7	435.0
人参氨基酸口服液 Ginseng Amino Acid Oral Liquid	人参 Ginseng	人参 Ginseng	99	EU592017.1	人参 Ginseng	97.4	447.0
人参三七含片 Ginseng and Sanqi Buccal Tablets	人参 Ginseng	人参 Ginseng	100	MG218783.1	人参 Ginseng	100.0	445.0
人参滴丸 Ginseng dropping pill	人参 Ginseng	人参 Ginseng	100	MG283292.11	人参 Ginseng	100.0	448.0
杞苓枣氨基酸口服液 Jujube Amino Acid Oral Liquid	枸杞子 Chinese wolfberry	枸杞子 Chinese	98	JQ320160	枸杞子 Chinese wolfberry	99.8	412.0
玛咖枸杞胶囊 Maca medlar capsule	枸杞子 Chinese wolfberry	枸杞子 Chinese	99	JQ320155	枸杞子 Chinese wolfberry	99.4	410.0
氨基酸枸杞片 Amino acid medlar tablet	枸杞子 Chinese wolfberry	枸杞子 Chinese	99	JQ320172	枸杞子 Chinese wolfberry	100.0	415.0
阿胶枸杞颗粒冲剂 Donkey hide gelatin and wolfberry granules	枸杞子 Chinese wolfberry	枸杞子 Chinese	98	JQ320160	枸杞子 Chinese wolfberry	98.9	416.0
森山铁皮枫斗颗粒 Senshan Iron Sheet Granules	铁皮石斛 Dendrobium candidum	铁皮石斛 Dendrobium candidum	97	YQ76839	铁皮石斛 Dendrobium candidum	98.7	457.0
九仙尊米斛枫斗浆 Nine Immortals Zun Rice Dendrobium Maple Juice	铁皮石斛 Dendrobium candidum	金钗石斛 Dendrobium nobile	98	UJ89765	金钗石斛 Dendrobium nobile	99.8	461.0
铁皮枫斗口服液 Dendrobium candidum oral liquid	铁皮石斛 Dendrobium candidum	铁皮石斛 Dendrobium candidum	99	YQ82313	铁皮石斛 Dendrobium candidum	100.0	452.0
津玉石斛口服液 Jinyu Dendrobium Oral Liquid	铁皮石斛 Dendrobium candidum	铁皮石斛 Dendrobium candidum	97	YQ78411	铁皮石斛 Dendrobium candidum	100.0	459.0
铁皮石斛西洋参黄芪胶囊 Dendrobium candidum, American ginseng and astragalus capsule	铁皮石斛 Dendrobium candidum	矩唇石斛 Dendrobium linawianum	99	QD53678	矩唇石斛 Dendrobium linawianum	98.2	423.0
红景天软胶囊 Rhodiola Soft Capsule	红景天 Rhodiola	红景天 Rhodiola	99	AY545716	红景天 Rhodiola	98.6	480.0
红景天西洋参口服液 Rhodiola and American Ginseng Oral Liquid	红景天 Rhodiola	轮叶景天 Sedum chauveaudii	100	EU592006	轮叶景天 Sedum chauveaudii	99.4	458.0
红景天提取物口服液 Rhodiola extract oral liquid	红景天 Rhodiola	红景天 Rhodiola	98	AB088628	红景天 Rhodiola	99.6	477.0
西洋参含片 American ginseng buccal tablet	红景天 Rhodiola	珠芽景天 Sedum bulbiferum	97	AY352897	珠芽景天 Sedum bulbiferum	98.7	481.0
美国洋参含片 American ginseng buccal tablet	红景天 Rhodiola	红景天 Rhodiola	98	AY545712	红景天 Rhodiola	99.0	469.0
Score: 离散匹配数值。 Score: Discrete matching value.

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