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猪圆环病毒2型复制酶蛋白的胞内互作蛋白研究进展

陈羽彤 田淑婧 陆春秀 苏春宇 吕其壮 黄维

陈羽彤,田淑婧,陆春秀,等. 猪圆环病毒2型复制酶蛋白的胞内互作蛋白研究进展 [J]. 福建农业学报,2023,38(8):1004−1010 doi: 10.19303/j.issn.1008-0384.2023.08.015
引用本文: 陈羽彤,田淑婧,陆春秀,等. 猪圆环病毒2型复制酶蛋白的胞内互作蛋白研究进展 [J]. 福建农业学报,2023,38(8):1004−1010 doi: 10.19303/j.issn.1008-0384.2023.08.015
CHEN Y T, TIAN S J, LU C X, et al. Research Progress on Intracellular Proteins Interacting with PCV2 Rep [J]. Fujian Journal of Agricultural Sciences,2023,38(8):1004−1010 doi: 10.19303/j.issn.1008-0384.2023.08.015
Citation: CHEN Y T, TIAN S J, LU C X, et al. Research Progress on Intracellular Proteins Interacting with PCV2 Rep [J]. Fujian Journal of Agricultural Sciences,2023,38(8):1004−1010 doi: 10.19303/j.issn.1008-0384.2023.08.015

猪圆环病毒2型复制酶蛋白的胞内互作蛋白研究进展

doi: 10.19303/j.issn.1008-0384.2023.08.015
基金项目: 国家自然科学基金项目(31860708);玉林师范学院大学生创新创业训练计划项目(202210606027)
详细信息
    作者简介:

    陈羽彤,主要从事分子病原学与免疫学相关研究,E-mail:c2922095838@163.com

    通讯作者:

    吕其壮(1989—),男,博士,教授,主要从事分子病原学与免疫学相关研究,E-mail:lvqizhuang062@163.com

    黄维(1990 —),女,博士,研究员,主要从事功能农业相关研究,E-mail:huangwei@ylu.edu.cn

  • 中图分类号: S853.65

Research Progress on Intracellular Proteins Interacting with PCV2 Rep

  • 摘要: 猪圆环病毒2型(Porcine circovirus virus type 2,PCV2)是引起断奶仔猪多系统衰竭综合征(Postweaning multisystemic wasting syndrome,PMWS)以及其他猪相关疾病的主要病原体。复制酶(Replicase,Rep)蛋白是由PCV2 ORF1基因编码的功能性复制启动蛋白,通过与ORF1编码的另一复制相关蛋白Rep′共同作用参与PCV2的DNA滚环复制。Rep蛋白作为PCV2的一种必需功能性蛋白,是研究PCV2抗病毒治疗的一个新突破口。然而,目前有关PCV2致病机理及疫苗的研究大多集中在Cap蛋白,对于Rep蛋白的作用机制研究较少。因此,本文结合国内外近年研究进展,对PCV2 Rep蛋白的胞内互作蛋白进行了系统分析,尤其对现今已发现的8种胞内互作蛋白在PCV2复制过程中可能发挥的重要作用及机制进行了阐释,并对其他可能与PCV2 Rep蛋白存在相互作用的胞内蛋白进行预测,以期为进一步阐明Rep蛋白在PCV2致病中的作用提供理论依据。
  • 图  1  已鉴定与Rep相互作用蛋白的生物信息学分析

    a:与Rep相互作用的胞内蛋白的功能分类。选择所有具有P值的GO类别作为标准。这些被鉴定出与Rep相互作用的蛋白质通过GO的富集类别进行分类。b:与Rep相互作用的胞内蛋白互作网络图;黄色菱形标记为中心蛋白Rep,红色三角形标记为与Rep蛋白相互作用的已识别蛋白,蓝色圆形标记为与已识别蛋白质相关的宿主细胞蛋白质。

    Figure  1.  Bioinformatics of identified proteins interacting with Rep

    a: Functional classification of identified proteins interacting with Rep. All GO categories with a P value were chosen as criteria. The identified proteins that interact with Rep were sorted by enrichment of GO categories. b: Rep–cellular protein interaction network. Central protein Rep is marked in yellow diamond; identified proteins interacting with Rep, in red triangles; and host cell proteins related to identified proteins, in blue circles.

    表  1  PCV2 Rep蛋白的胞内互作蛋白

    Table  1.   Intracellular proteins interacting with PCV2 Rep

    互作蛋白
    Interacting
    protein
    功能
    Function
    参考文献
    Reference
    Cap参与PCV2组装[12]
    Rep′参与PCV2复制[12]
    Syncoilin介导病毒DNA转运和分离[12]
    c-Myc促进细胞分裂[12]
    ZNF265调节胞内转录和剪接[13]
    VG5Q促进血管生成[13]
    TDG调节基因转录[13]
    HMGCR参与脂质、固醇的合成、蛋白质的代谢和PCV2的复制[11]
    下载: 导出CSV
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  • 收稿日期:  2022-12-23
  • 修回日期:  2023-04-29
  • 刊出日期:  2023-08-28

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