高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响

乔利; 张天海; 耿书宝; 金银利; 张方梅; 武予清; 潘鹏亮

doi:10.19303/j.issn.1008-0384.2023.11.010

高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响

doi: 10.19303/j.issn.1008-0384.2023.11.010

1.
信阳农林学院农学院，河南　信阳　464000
2.
河南省农业科学院植物保护研究所，河南　郑州　450002

基金项目: 河南省重点研发与推广专项（科技攻关）（222102110109）；国家重点研发计划（2017YFD0200907）；河南省青年科学基金项目（212300410229）；信阳农林学院科技创新团队项目（XNKJTD-007）

详细信息

作者简介:
乔利（1980 — ），女，博士，副教授，主要从事农业昆虫与害虫防治研究，E-mail：2017180004@xyafu.edu.cn

通讯作者:
潘鹏亮（1976 — ）男，博士，副教授，主要从事昆虫与害虫防治研究，E-mail：2014180001@xyafu.edu.cn

中图分类号: S433.3
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- 被引次数: 0
出版历程
- 收稿日期: 2023-06-06
- 修回日期: 2023-07-31
- 网络出版日期: 2023-12-21
- 刊出日期: 2023-11-28

Effects of High Temperature on Lifespan, Fecundity, and Protective Enzymes of Ectropis grisescens

1.
College of Agriculture, Xinyang Agriculture and Forestry University, Xinyang, Henan　464000, China
2.
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan　450002, China

摘要

摘要: 目的明确灰茶尺蠖（Ectropis grisescens Warren）对高温的耐受性及其体内抗氧化系统对高温胁迫的响应机制。方法以25 ℃为对照，灰茶尺蠖雌、雄成虫经高温 30、35、38 ℃和41 ℃处理1 h，44 ℃处理3 min后，测定其寿命、产卵量、卵孵化率及其体内总蛋白含量、超氧化物歧化酶（Superoxide dismutas，SOD）、过氧化氢酶（Catalase，CAT）、过氧化物酶（Peroxidase，POD）的活性和总抗氧化（Total antioxidant capacity，T-AOC）能力的变化。结果随着胁迫温度的增加，灰茶尺蠖雌、雄成虫的寿命缩短，雌成虫的产卵量逐渐下降，卵孵化率降低。41 ℃和44 ℃时，卵孵化率为0。灰茶尺蠖雌、雄成虫总蛋白含量分别在35 ℃和38 ℃时达最高值，均显著高于其他处理，且雌、雄间差异显著。雌成虫SOD活性呈“低-高-低”的趋势，雄成虫SOD活性41 ℃处理较低。雌成虫在44 ℃时CAT活性最高，T-AOC含量随温度的增加逐渐降低，雌成虫的POD活性随温度升高逐渐增加，雄成虫呈“低-高-低”的趋势。结论灰茶尺蠖体内保护酶活性在适应高温胁迫过程中发挥了重要作用。
- 灰茶尺蠖 /
- 保护酶 /
- 产卵量 /
- 孵化率 /
- 高温
Abstract: Objective Resistance and response to high temperatures of the antioxidants in Ectropis grisescens were studied. Method Lifespan and fecundity of adult E. grisescens Warren after being exposed to 30 ℃, 35 ℃, 38 ℃ or 41 ℃ for 1 h or 44 ℃ for 3 min were observed according to measurements on the egg hatchability and total protein content and activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) as well as total antioxidant capacity (T-AOC) in the insects. Result The increased temperature significantly shortened the lifespan, decreased the female oviposition, and lowered the egg hatchability of E. grisescens. At 41 ℃ and 44 ℃, the egg ceased to hatch. The maximum total protein contents in the female and male adults differed significantly under different temperatures—it was under 35 ℃ for the females and 38 ℃ for the males. The SOD activity in the female adults changed in an upside-down V pattern as the temperature rose, while that in the male adults was low at 41 ℃. In the female adults the highest CAT activity occurred at 44 ℃, the T-AOC decreased with increasing temperature, and the POD rose with rising temperature, whereas those in the male adults exhibited a low-high-low trend in the temperature range. Conclusion The protective enzymes in E. grisescens Warren played a key role in how the insect adapted to high temperature stress.
- Ectropis grisescens Warren /
- protective enzymes /
- oviposition /
- hatchability /
- high temperature

HTML全文

图 1 温度胁迫对灰茶尺蠖的产卵量的影响

不同小写字母表示不同温度处理间的测定值达显著差异（P＜0.05）。图2~8同。

Figure 1. Effect of high temperature on oviposition of E. grisescens

Data with different lowercase letters indicate significant differences in measured values at different temperature treatments (P＜0.05). Same for Figures 2-8.

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图 2 温度胁迫后灰茶尺蠖的孵化率的影响

Figure 2. Effect of high temperature on hatchability of E. grisescens eggs

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图 3 温度胁迫对灰茶尺蠖寿命的影响

不同小写字母表示雌或雄成虫不同温度处理间的差异显著性（P＜0.05）。不同大写字母表示同一温度雌、雄成虫处理间的差异显著性（P＜0.05）。下图同。

Figure 3. Effect of high temperature on lifespan of E. grisescens

Those with different lowercase letters indicate significant differences between male and female adults at differert temperature (P＜0.05); those with different capital letters indicate significant difference between male and female adults at the same temperature (P＜0.05). Same for below.

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图 4 温度胁迫对灰茶尺蠖总蛋白含量的影响

Figure 4. Effect of high temperature on total protein in E. grisescens

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图 5 温度胁迫对灰茶尺蠖SOD活性的影响

Figure 5. Effect of high temperature on SOD activity in E. grisescens

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图 6 温度胁迫对灰茶尺蠖CAT活性的影响

Figure 6. Effect of high temperature on CAT activity

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图 7 温度胁迫对灰茶尺蠖POD活性的影响

Figure 7. Effect of high temperature on POD activity in E. grisescens

下载: 全尺寸图片幻灯片

图 8 温度胁迫对灰茶尺蠖T-AOC的影响

Figure 8. Effect of high temperature on T-AOC in E. grisescens

下载: 全尺寸图片幻灯片

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