抗病优质杂交稻品种野香优699的选育与应用

朱永生; 魏毅东; 蒋家焕; 李齐向; 董瑞霞; 郑菲艳; 陈丽萍; 王晓方; 谢华安; 张建福

doi:10.19303/j.issn.1008-0384.2023.12.003

抗病优质杂交稻品种野香优699的选育与应用

doi: 10.19303/j.issn.1008-0384.2023.12.003

1.
福建省农业科学院水稻研究所/闽台作物有害生物生态防控国家重点实验室/农业农村部华南杂交水稻种质创新与分子育种重点实验室/福州（国家）水稻改良分中心/水稻国家工程实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/杂交水稻国家重点实验室华南研究基地，福建　福州　350003
2.
三明市种子站，福建　三明　365000

基金项目: 福建省科技计划重大专项（2022NZ030014）；福建省农业高质量发展超越“5511”协同创新工程项目（XTCXGC2021019-SDS01）；福建省农业科学院科技创新团队建设项目（CXTD2021005-3）

详细信息

作者简介:
朱永生（1982 — ），男，博士，助理研究员，主要从事水稻遗传育种研究，E-mail：zysfaas@qq.com

通讯作者:
张建福（1971 — ），男，博士，研究员，主要从事水稻分子育种研究， E-mail：jianfzhang@163.com

中图分类号: S511
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出版历程
- 收稿日期: 2023-11-09
- 修回日期: 2023-11-29
- 网络出版日期: 2024-01-25
- 刊出日期: 2023-12-28

Breeding and Properties of Hybrid Rice Yexiangyou 699

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Academy of Agricultural Sciences/Key Laboratory of hybrid rice Germplasm Enhancement and Molecular Breeding in South China, Ministry of Agriculture, P.R China/Fuzhou branch, National Rice Improvement Center of China/National Engineering Laboratory of Rice/ Incubator of Key Laboratory of Crop Germplasm Enhancement and Molecular Breeding between Fujian and Ministry of Sciences and Technology/ Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding/Resaerch Base of South-China of National Key Laboratory of Hybrid Rice of China, Fuzhou , Fujian　350003, China
2.
Sanming Seed Station,Sanming, Fujian　365000, China

摘要

摘要: 目的选育优质、抗病的杂交稻品种在生产上推广和应用，满足市场需求，提高种粮效益。方法通过水稻优异基因单倍型背景分析，以产量高、抗病性好、恢复力强、配合力好的恢复系福恢699为父本，与香型、优质、抗病的三系不育系野香A杂交配组，杂种F₁代经多年多点稻瘟病圃抗性鉴定，多生态区适应性筛选和稻米品质分析等相结合，选育符合育种目标性状的优良杂交稻品种。结果 2017—2018年参加福建省中稻区域试验，表现群体整齐，株型适中，全生育期两年平均140.4 d，比对照II优3301早熟2.8 d。有效穗数208.5万穗·hm⁻²，平均株高135.4 cm，结实率86.89%，千粒重26.3 g。产量平均9404.3 kg·hm⁻²，比对照增产1.50%，不显著；野香优699聚合了来自双亲的7个稻瘟病抗性基因和2个水稻病毒病抗性基因，两年区试田间和室内稻瘟病抗性鉴定综合评价均抗稻瘟病，生产上也表现较强的抗病性。结论野香优699（闽审稻20200012）糙米率80.1%，整精米率68.5%，垩白度0.8%，透明度1级，碱消值5.5，胶稠度86 mm，直链淀粉含量14.0%，米质达部颁三等优质食用稻品种品质标准，田间表现产量高、抗病性强，在生产上可大面积推广应用。
- 抗稻瘟病 /
- 杂交稻 /
- 野香优699 /
- 优质
Abstract: Objective A disease-resistant, high-quality hybrid rice was bred to meet the market demand and improve the profitability for the farmers. Method Based on the desired gene haplotypes, the high-yield, disease-resistant, strong-restoring and combining Fuhui 699 restorer line was selected as the male parent to cross with the female parents, the 3 sterile Yexiang A lines that are known for their desirable fragrance, high quality, and strong disease-resistance. The resulting F₁ hybrid was examined for the target traits at multi-year and multi-point rice blast resistance tests as well as multi-ecological adaptability and rice quality analyses. Result From 2017 to 2018, the choice hybrid, Yexiangyou 699, was submitted to the regional trial on mid-season rice in Fujian. It displayed an evenly distributed population of acceptable plant type and delivered a two-year averaged growth period of 140.4 d, which was 2.8 d shorter than the reference, II You 3301. The effective panicle number of the cultivar averaged 2.085 million panicles·hm⁻², the plant height 135.4 cm, the seed setting rate 86.89%, and the 1000-grain weight 26.3 g. The average crop yield was 9 404.3 kg·hm⁻², which was 1.50% higher than that of control. The hybrid inherited 7 blast-resistance and two virus-resistance genes from the parents rendering it blast-free in the field and laboratory for 2 years. Conclusion The rates of brown and milled rice of Yexiangyou 699 (now officially identified as Min Approved Rice No. 20200012) were 80.1% and 68.5%, respectively. The grain quality indicators of the hybrid included 0.8% on chalkiness, Grade 1 on transparency, 5.5 on alkali elimination, 86 mm on gelatinized consistency index, 14.0% on amylopectin content, 3^rd grade on the high-quality edible rice set by the Ministry, and high yield and strong disease-resistance in the field. Yexiangyou 699 was recommended as a new breed for cultivation.
- Rice blast resistant /
- hybrid rice /
- Yexiangyou 699 /
- high quality

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图 1 福恢699选育过程

Figure 1. Breeding of Fuhui 699

下载: 全尺寸图片幻灯片

图 2 野香优699及其双亲Badh2基因的测序分析

Figure 2. Sequences of Badh2 in Yexiangyou 699 and parents

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图 3 野香优699的父、母本及其杂种F₁的背景、籼粳片段分析

A、B、C为基因组背景纯度，D、E、F为籼粳片段分析；FA-1为野香A，FA-3为福恢699，FA-5为野香优699。

Figure 3. Background and Indica japonica fragments of parents and hybrid F₁ of Yexiangyou 699

A, B, and C: genomic background purity; D, E, and F: I. japonica fragments; FA-1: Yexiang A; FA-3: Fuhui 699; FA-5: Yexiangyou 699.

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表 1 2017—2019年野香优699在福建省中稻区域试验及生产试验中的产量

Table 1. Yield of Yexiangyou 699 in regional and production trials of mid-season rice in Fujian from 2017 to 2019

试验组别 Type of trial	年份 Year	品种 Varieties	生育期 Growth period/d	生育期比CK± Compared with CK/d	产量 Yield/（kg·hm⁻²）	增产率 Ratio compared with CK/%
区域试验 Regional trial	2017	野香优699 Yexiangyou 699	141.3	−2.0	9723.3 a	−0.20
	2017	II优3301 II you 3301(CK)	143.3	0	9742.8 a	0
	2018	野香优699 Yexiangyou 699	139.5	-3.6	9085.4 b	3.20
	2018	II优3301 II you 3301(CK)	143.1	0	8803.6 a	0
生产试验 Producing trial	2019	野香优699 Yexiangyou 699	140.4	−2.8	8574.0	10.11
生产试验 Producing trial	2019	II优3301 II you 3301(CK)	143.2	0	7786.8	0
同列数据后不同小写字母表示同一年份不同处理间差异显著（P＜0.05）。Ｄata with different lowercase letters on the same column indicate significant differences between different treatments in the same year (P＜0.05).

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表 2 野香优699稻瘟病抗性鉴定结果

Table 2. Identification on rice blast disease resistance of Yexiangyou 699

年份 Year	地点 location	田间鉴定 Identification in field											室内鉴定 Identification in greenhouse	抗性综评 Comprehensive evaluation of resistance
		叶瘟 Leaf blast				穗颈瘟 Panicle blast					综评 Comprehensive evaluation		室内鉴定 Identification in greenhouse
		发病率 Incidence rate%	病情指数 Disease index	最高级 Top grade	抗感表现 Resistance evaluation	发病率 Incidence rate%	发病率病级 Grade of Incidence rate	病情指数 Disease index	最高级 Top grade	抗感表现 Resistance evaluation	综评 Comprehensive evaluation	抗菌株率 Resistance to strain rate/ %	抗感表现 Resistance evaluation
2017	将乐 Jiangle	1.5	0.22	2	R	14.00	5	1.56	1	R	R	94.45	R	R
	宁化 Ninghua	2.00	0.22	1	R	0.00	1	0.00	0	R	R
	上杭 Shanghang	6.50	1.83	3	R	4.50	1	1.39	5	R	R
	建阳 Jianyang	0.00	0.00	0	R	0.00	1	0.00	5	R	R
	综合 Average	2.25	0.54	3	R	4.63	1	0.74	5	R	R
2018	将乐 Jiangle	38.50	7.33	2	MR	19.00	5	3.00	3	R	MR	93.75	R	R
	宁化 Ninghua	3.00	0.39	2	R	2.50	1	0.94	5	R	R
	上杭 Shanghang	5.50	1.56	3	R	5.50	3	1.50	5	R	R
	建阳 Jianyang	0.00	0.00	0	R	21.00	5	5.67	3	MR	MR
	综合 Average	11.75	2.32	3	R	12.00	5	2.78	5	R	R

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表 3 野香优699的父、母本及其杂种F₁的籼粳片段统计分析

Table 3. Statistical analysis on I. Japonica fragments of parents and hybrid F₁ of Yexiangyou 699

品种 Varieties	区段总数 Total fragments	粳稻区段数 Japonica fragments	粳稻比例 Japonica proportion/%	籼稻区段数 Indica fragments	籼稻比例 Indica proportion/%	不能判断区段数 Undetermine fragments
野香A Yexiang A	750	76	10.13	523	69.73	151
福恢699 Fuhui 699	750	78	10.40	513	68.40	159
野香优699 Yexiangyou 699	750	77	10.27	565	75.33	108

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表 4 野香优699及其亲本的抗病基因检测

Table 4. Detection of disease resistance genes in Yexiangyou 699 and parents

序号 No	基因名称 Gene	探针类型 Probe	类型 Type	染色体 Chr	对照品种 Standard variety	基因功能 Gene function	鉴定品种 Varieties
序号 No	基因名称 Gene	探针类型 Probe	类型 Type	染色体 Chr	对照品种 Standard variety	基因功能 Gene function	野香A Yexiang A	福恢699 Fuhui 699	野香优699 Yexiangyou 699
1	Rymv1	SNP	抗生物逆境	4	Nipponbare	抗黄色斑驳病毒病	√	√	√
2	STV11	SNP	抗生物逆境	11	Kasalath	抗水稻条叶枯病毒	√	√	√
3	Pi1	单倍型	抗生物逆境	11	10SNP	稻瘟病抗性		√	H
4	Pi5	单倍型	抗生物逆境	9	33SNP	稻瘟病抗性		√	H
5	Pi9	单倍型	抗生物逆境	6	80SNP	稻瘟病抗性		√	H
6	Pia	单倍型	抗生物逆境	11	13SNP	稻瘟病抗性		√	H
7	Pid2	单倍型	抗生物逆境	6	66SNP	稻瘟病抗性	√	√	√
8	Pid3	单倍型	抗生物逆境	6	64SNP	稻瘟病抗性	√	√	√
9	Pita	单倍型	抗生物逆境	12	32SNP	稻瘟病抗性	√		H
“√”表示所检测品种的基因型与对照品种基因型一致；“H”表示品种中该基因为杂合型。 '√' indicates genotype of tested variety to be consistent with that of control;'H' indicates heterozygous gene.

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