香型优质高产杂交稻新品种野香优669的选育与应用

朱永生; 魏毅东; 李齐向; 郑菲艳; 蔡秋华; 董瑞霞; 陈丽萍; 蒋家焕; 吴方喜; 谢华安; 张建福

doi:10.19303/j.issn.1008-0384.2024.01.004

香型优质高产杂交稻新品种野香优669的选育与应用

doi: 10.19303/j.issn.1008-0384.2024.01.004

1.
福建省农业科学院水稻研究所/闽台作物有害生物生态防控国家重点实验室/农业农村部华南杂交水稻种质创新与分子育种重点实验室/福州（国家）水稻改良分中心/水稻国家工程实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/杂交水稻国家重点实验室华南研究基地，福建　福州　350003
2.
三明市种子站，福建　三明　365000

基金项目: 福建省农业科学院科技创新团队建设项目（CXTD2021005-3）；福建省科技重大专项（2022NZ030014）；福建省农业高质量发展超越“5511”协同创新工程项目（XTCXGC2021019-SDS01）

详细信息

作者简介:
朱永生（1982 —），男，博士，助理研究员，主要从事水稻遗传育种研究，E-mail：zysfaas@qq.com

通讯作者:
张建福（1971 —），男，博士，研究员，主要从事水稻分子育种研究，E-mail：jianfzhang@163.com

中图分类号: S511
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出版历程
- 收稿日期: 2023-11-24
- 修回日期: 2024-01-04
- 网络出版日期: 2024-01-25
- 刊出日期: 2024-01-28

Breeding an Aromatic, High-quality, High-yield Hybrid Rice Yexiangyou 669

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Academy of Agricultural Sciences/Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding in South China, Ministry of Agriculture, P.R. China/Fuzhou branch, National Rice Improvement Center of China/National Engineering Laboratory of Rice/Incubator of Key Laboratory of Crop Germplasm Enhancement and Molecular Breeding between Fujian and Ministry of Sciences and Technology/ Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Research Base of South-China of National Key Laboratory of Hybrid Rice of China, Fuzhou, Fujian　350003, China
2.
Sanming Seed Station, Sanming, Fujian　365000, China

摘要

摘要: 目的选育产量、米质和抗性相协调的香型优质水稻新品种。方法利用亲本间的遗传多样性和农艺性状的互补性，以高产、高配合力的父本福恢669与香型、优质、抗病的不育系野香A杂交，结合多生态点抗性鉴定、高温逼熟后品质分析、多年多点品比试验等相结合。结果选育出优质、高产、抗病，株叶态协调的杂交稻品种野香优669。 2019–2020年参加福建省中稻区域试验，全生育期两年平均139.8 d, 比对照II优3301早熟1.1 d，产量平均9363 kg·hm⁻²，比对照增产2.24%，未达显著水平。两年稻瘟病抗性鉴定综合评价为抗稻瘟病，稻米品质达部颁一等优质食用稻品种品质标准，2021年通过福建省农作物审定委员会审定（闽审稻20210014）。野香优669聚合了来自双亲的22个有利基因，其中6个为单方携带，16个为双亲均携带。结论野香优669产量高、抗性强、品质好、口感佳，在生产上大面积推广应用，是福建省农业主推品种之一。
- 杂交水稻 /
- 香稻 /
- 野香优669 /
- 优质 /
- 抗病
Abstract: Objective An aromatic, high-quality, high-yield, disease-resistant rice variety was bred. Method Utilizing the genetic diversity and complementing agronomic traits between the male parent, Fuhui 669 with high yield and combining ability, and the female parent, the fragrant, high-quality, disease-resistant sterile line Yexiang A, a new breed of rice were generated. The hybrid was subjected to multiple ecological resistance identification, a quality analysis after high temperature maturation, and years of multi-point comparison test prior to final selection. Result The high-quality, high-yield, disease-resistant Yexiangyou 669 of a desirable plant type was obtained and submitted to the regional trial for mid-season rice in Fujian from 2019 to 2020. In the 2-year trial, it showed an average growth period of 139.8 d, which was 1.1 d shorter than the reference cultivar, II You 3301, and an average yield of 9 363 kg·hm⁻², which was 2.24% higher than control but not statistically significant. It exhibited resistance to rice blast and up to the national Grade A quality. In 2021, it was certified by the Fujian Provincial Crop Approval Committee as the Min Approved Rice No. 20210014. Yexiangyou 669 had inherited 6 desirable genes from one parent and 16 common to both parents. Conclusion The new hybrid rice Yexiangyou 669 had high yield, strong disease resistance, and superb grain quality and was promoted as one of the main varieties in the province.
- Hybrid rice /
- aromatic rice /
- Yexiangyou 669 /
- high quality /
- disease resistance

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图 1 福恢669选育过程

Figure 1. Breeding process of Fughui 669

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表 1 2019–2020年野香优669在福建省中稻区域试验及生产试验中的生育期与产量

Table 1. Yield and growth period of Yexiangyou 669 in 2019–2020 regional trial and production test for mid-season rice in Fujian

试验组别 Type of trial	年份 Year	品种 Varities	生育期 Growth period/d	比CK增加 Compare with CK/d	产量 Yield/（kg·hm⁻²）	增产率 Ratio compared with CK/%
区域试验 Regional trial	2019	野香优669 Yexiangyou 669	141.1	−0.8	9241.5	2.40
	2019	II优3301（CK） II You 3301（CK）	141.9	—	9024.9	—
	2020	野香优669 Yexiangyou 669	138.5	−1.4	9484.4	2.08
	2020	II优3301（CK） II You 3301（CK）	139.9	—	9291.1	—
生产试验 Producting trial	2020	野香优669 Yexiangyou 669	138.4	−1.1	8642.9	1.59
生产试验 Producting trial	2020	II优3301（CK） II You 3301（CK）	139.5	—	8507.6	—

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表 2 野香优669稻瘟病抗性鉴定结果

Table 2. Blast-resistance of Yexiangyou 669 certified at nurseries

年份 Year	试验地点 Location	田间鉴定 Identification in field									室内鉴定 Identification in greenhouse		综合评价 Comprehensive evaluation
		苗叶瘟 Leaf blast		穗茎瘟 Panicle blast				综合指数 Composite index	穗损失率最高级别 Top grade of loss rate	抗感类型 Resistance grade	抗菌株率 Resistance rate/%	抗感类型 Resistance evaluation
		最高级 Top grade	平均病级 Average grade	发病率 Incidence rate/%	发病率级别 Grade of Incidence rate	损失率 Loss rate/%	损失率级别 Grade of loss rate	综合指数 Composite index	穗损失率最高级别 Top grade of loss rate	抗感类型 Resistance grade	抗菌株率 Resistance rate/%	抗感类型 Resistance evaluation
2019	上杭 Shanghang	4	3	10.50	5	3.53	1	2.50	1	R	94.12	R	R
	建阳 Jianyang	3	2	23.50	5	3.95	1	2.25		R
	将乐 Jiangle	0	0	0.00	0	0.00	0	0.00		HR
	宁化 Ninghua	2	1	11.50	5	2.08	1	2.00		R
	综合 Average	4	1.5	11.38	3.75	2.39	0.75	1.69		R
2020	上杭 Shanghang	5	4	3.00	1	0.45	1	1.75	1	R	90.91	R	R
	建阳 Jianyang	3	2	17.00	5	3.10	1	2.25		R
	将乐 Jiangle	0	0	0.00	0	0.00	0	0.00		HR
	宁化 Ninghua	3	2	5.00	1	0.70	1	1.25		R
	综合 Average	5	2	6.25	1.75	1.06	1	1.31		R

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表 3 野香优669及其亲本的有利基因检测

Table 3. Desirable genes identified in Yexiangyou 669 and its parents

序号 No.	基因名称 Gene name	探针类型 Probe type	基因类型 Gene type	染色体 Chromosome	基因功能 Gene function	检测品种 Test variety
序号 No.	基因名称 Gene name	探针类型 Probe type	基因类型 Gene type	染色体 Chromosome	基因功能 Gene function	野香A Yexiang A	福恢669 Fuhui 669	野香优669 Yexiangyou 669
1	Gn1a	SNP	产量	1	每穗粒数增加	√	√	√
2	OsSPL16	INDEL	产量	8	高产		√	H
3	SKC1	SNP	抗非生物逆境	1	耐盐	√	√	√
4	NRT1.1B	SNP	抗非生物逆境	10	增强氮吸收	√	√	√
5	Rymv1	SNP	抗生物逆境	4	抗病，抗黄色斑驳病毒病	√	√	√
6	STV11	SNP	抗生物逆境	11	持久抗水稻条纹叶枯病毒		√	H
7	Pi5	单倍型	抗生物逆境	9	稻瘟病抗性		√	H
8	Pia	单倍型	抗生物逆境	11	稻瘟病抗性		√	H
9	Pid2	单倍型	抗生物逆境	6	稻瘟病抗性	√	√	√
10	Pid3	单倍型	抗生物逆境	6	稻瘟病抗性	√	√	√
11	Pita	单倍型	抗生物逆境	12	稻瘟病抗性	√		H
12	GW2	SNP	品质	2	大粒	√	√	√
13	GS3	SNP	品质	3	长粒	√	√	√
14	OsCYP704A3	SNP	品质	4	长粒	√	√	√
15	Chalk5	SNP	品质	5	垩白度低，高质量	√	√	√
16	Waxy	SNP	品质	6	非糯的情况下，增加支连淀粉含量	√	√	√
17	ALK	SNP	品质	6	糊化温度升高	√	√	√
18	Hd3a	SNP	生育期	6	促进抽穗	√	√	√
19	SaF	SNP	育性	1	广亲和（粳稻亲和）	√		H
20	S5	SNP	育性	6	广亲和（籼稻亲和）	√	√	√
21	Sd-1	INDEL	株型	1	半矮秆	√	√	√
22	Sdt97	SNP	株型	6	半矮秆	√	√	√
“√”表示所检测品种的基因型与标准品种基因型一致；“H”表示品种中该基因为杂合型。 "√" indicates consistency between genotypes of tested and standard varieties; "H" indicates heterozygous genes.

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表 4 2021–2023年野香优669在福建省建立示范片的面积及产量

Table 4. Square footage and yield of cultivation demonstration on Yexiangyou 669 in 2021–2023

年份 Year	示范地点 Demonstration location	示范面积 Demonstration area/ hm²	测产产量 Test yield/ （kg·hm⁻²）
2021	武夷山市吴屯乡	13.3	9043.8
2021	德化县杨梅乡	8.0	未测产
2022	尤溪县管前镇	7.41	13154.1
2022	明溪县城关乡	13.9	10945.2
2022	德化县杨梅乡	8.0	未测产
2022	光泽县鸾凤乡	13.3	10273.5
2023	上杭县泮境乡	8.0	9524.0
2023	德化县杨梅乡	8.0	未测产
平均 Average			10588.1

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