1992 Vol. 7, No. 1
Display Method:
1992, 7(1): 1-8.
Abstract:
The Fujian subtropical mountain area is undulate and there are very kinds of climatic and soil tpyes.The average temperature of ten-day,the annual amount of solar radiation and the duration and cumulative temperature of rice growth showed decreasing with the rising of latitude and altitude.Their decreasibg ratio differed with date.According to the time and space distribution pattern of temperature and light resources,the rice climatic eco-type can be divided into three climatic types the warm climatic layer,the temperate climatic layer and the cool climatic layer.The most of Fujian mountain and hilly area rice soil developed from red soil.Being affected by the long time natural condition,they formed three soil types of hillside yellow soil,mountain conyon gleyed soil and rive side senddy alluvium soil.The three type soils have different phsical and chemical characteristics.Basing on the classification of rice ecology,through researching the model of cropping system,varieties used and growth season of rice cultivated in these three climatic layer regions,the system of improving fertility of the three type paddy soils and the high yielding cultivation technical system for hybrid rice have been layouted.The technical system has been applied all over the mountain and hilly areas in Fujian,and increased production greetly year by year.
The Fujian subtropical mountain area is undulate and there are very kinds of climatic and soil tpyes.The average temperature of ten-day,the annual amount of solar radiation and the duration and cumulative temperature of rice growth showed decreasing with the rising of latitude and altitude.Their decreasibg ratio differed with date.According to the time and space distribution pattern of temperature and light resources,the rice climatic eco-type can be divided into three climatic types the warm climatic layer,the temperate climatic layer and the cool climatic layer.The most of Fujian mountain and hilly area rice soil developed from red soil.Being affected by the long time natural condition,they formed three soil types of hillside yellow soil,mountain conyon gleyed soil and rive side senddy alluvium soil.The three type soils have different phsical and chemical characteristics.Basing on the classification of rice ecology,through researching the model of cropping system,varieties used and growth season of rice cultivated in these three climatic layer regions,the system of improving fertility of the three type paddy soils and the high yielding cultivation technical system for hybrid rice have been layouted.The technical system has been applied all over the mountain and hilly areas in Fujian,and increased production greetly year by year.
1992, 7(1): 9-13.
Abstract:
The genetic variation coefficients,broad heritabilities.genetic advances,correlation coefficients,correlative heritabilities and path coefficients of main quantitative characters of the selective peanut germplasm resources in Fujian were studied.The results showed that genetic variation coefficients were larger in most characters and different among the characters.The heritabilities were also different with various characters and the genetic advances of most characters were large,indicating that significative effect should be obtained to select on these characters with a larger gentle advance.There were positive correlations among characters studied with the pod yield per plant and the pod yield per plot.Path analysis indicated that direct path coerricient was more signifocant in the 100-pod weight to the pod yield per plant positively.Direct path coerricient was also signigicant in the mature pod per plant to the pod yield per plant and showed positive value.However,direct effect was minor in the number of fruit branches to the pod yield per plant.The performence of peanut single plants in phenotype under Fujian ecological conditions also be discussed.
The genetic variation coefficients,broad heritabilities.genetic advances,correlation coefficients,correlative heritabilities and path coefficients of main quantitative characters of the selective peanut germplasm resources in Fujian were studied.The results showed that genetic variation coefficients were larger in most characters and different among the characters.The heritabilities were also different with various characters and the genetic advances of most characters were large,indicating that significative effect should be obtained to select on these characters with a larger gentle advance.There were positive correlations among characters studied with the pod yield per plant and the pod yield per plot.Path analysis indicated that direct path coerricient was more signifocant in the 100-pod weight to the pod yield per plant positively.Direct path coerricient was also signigicant in the mature pod per plant to the pod yield per plant and showed positive value.However,direct effect was minor in the number of fruit branches to the pod yield per plant.The performence of peanut single plants in phenotype under Fujian ecological conditions also be discussed.
1992, 7(1): 14-22.
Abstract:
Sandy culture experiments were carried out to study the effects of the length of low temperature treatment time (0,3,6,9 hr.) on the seedcane,bud germination and seedling quality.The main results were as fellows; 1.The cane buds were softed,water-logged and destroyed after low tempterature treatments,especially in the 9 hr.treatment.However,the root primordium appeared not to be damaged.On the other hand,acid invertase activity increased and the reducing sugar content was raised for the low temperature treatment in the seedcane.2.The menifestation of lowered seedling quality,such as dwarf ness of plant and decreased numbers of leaves,was observed with the increase length of the low temperature treatment time in the seedcane.It was revealed from the principal component analysis that a well developed root system was fundermental for the high yield because of the stronger effect of low temperature on shoot than on root.3.By means of regression simulation of nine curves,germinating potential coincided with three times curves.Bud sprout slowed and the germinaring percentage decreased with the low temperature treatment time lengthed.
Sandy culture experiments were carried out to study the effects of the length of low temperature treatment time (0,3,6,9 hr.) on the seedcane,bud germination and seedling quality.The main results were as fellows; 1.The cane buds were softed,water-logged and destroyed after low tempterature treatments,especially in the 9 hr.treatment.However,the root primordium appeared not to be damaged.On the other hand,acid invertase activity increased and the reducing sugar content was raised for the low temperature treatment in the seedcane.2.The menifestation of lowered seedling quality,such as dwarf ness of plant and decreased numbers of leaves,was observed with the increase length of the low temperature treatment time in the seedcane.It was revealed from the principal component analysis that a well developed root system was fundermental for the high yield because of the stronger effect of low temperature on shoot than on root.3.By means of regression simulation of nine curves,germinating potential coincided with three times curves.Bud sprout slowed and the germinaring percentage decreased with the low temperature treatment time lengthed.
1992, 7(1): 23-29.
Abstract:
Rhizobium was introduced into rice root for nodulation and symbiosis by the addition of synthetic auxin 2,4-D (2,4-Dichlorophenoxyacetic acid) (final concentration was Ippm).A number of upland rice and some lowland rice varieties have been treated since 1988.The results showed that rhizobium can be introduced into rice root to nodulate and symbiosis with rice by this method.The repetition and reappearance of inducing nodulation were very well.The date of the first nodule appearance,nodulation rate and size of nodules vavied with different rice varieties.Upland rice varieties of Fan 289033,Futiannuo and Youhan showed higher nodulation rates with larger nodule size.The diameter of nodules was about 2 to 3 mm four weeks after inoculation.Fan 289033 with highest nodulation rate(reached to 85%)anong 10 varieties tested.Nodule sections of rice were observed under electron microscope.The results showed that there were numerous rhizobia inside the nodule,indicating that rhizobium can enter rice root and propagated in the nodule.The novel association between rhizobium and rice plant revealed here may be important to the research on nodulation and nitroge fixation of non-legume plants.
Rhizobium was introduced into rice root for nodulation and symbiosis by the addition of synthetic auxin 2,4-D (2,4-Dichlorophenoxyacetic acid) (final concentration was Ippm).A number of upland rice and some lowland rice varieties have been treated since 1988.The results showed that rhizobium can be introduced into rice root to nodulate and symbiosis with rice by this method.The repetition and reappearance of inducing nodulation were very well.The date of the first nodule appearance,nodulation rate and size of nodules vavied with different rice varieties.Upland rice varieties of Fan 289033,Futiannuo and Youhan showed higher nodulation rates with larger nodule size.The diameter of nodules was about 2 to 3 mm four weeks after inoculation.Fan 289033 with highest nodulation rate(reached to 85%)anong 10 varieties tested.Nodule sections of rice were observed under electron microscope.The results showed that there were numerous rhizobia inside the nodule,indicating that rhizobium can enter rice root and propagated in the nodule.The novel association between rhizobium and rice plant revealed here may be important to the research on nodulation and nitroge fixation of non-legume plants.
1992, 7(1): 30-35.
Abstract:
The effects of Anabaena azollae on the heat tolerance of Azolla were conducted.Under optimum temperature regine anabaena-free Azolla grown in nitrogen-containing medium produced less biomass compared with Azolla-Anabaena association.It implied that Anabnena in the association was not only supply nitrogen to Azolla but also provied something else which was required for Azolla growth.At 37℃/29℃ (day/night) Azolla filiculoides grew poor than the corresponding Anabaena-tree Azolla grown in nitrogen-containing medium while Azolla filiculoides with the Anabaena from Azolla microfhylla (Af 135),a newly reconstituting Azolla,was able to grow much better than the Azolla with homologous Anabaena.However,Azolla microfhylla with the Anabaena from Azolla filiculoides grew and fixed nitrogen less than original one.the growth of Azlolla microfhylla was much better than Azolla filicwifos with the Anabaena from Azolla microfhylla.Therefore,the tolerance of Azolla to high temperature is determined by both host-fern and symbiont-Anabaena.
The effects of Anabaena azollae on the heat tolerance of Azolla were conducted.Under optimum temperature regine anabaena-free Azolla grown in nitrogen-containing medium produced less biomass compared with Azolla-Anabaena association.It implied that Anabnena in the association was not only supply nitrogen to Azolla but also provied something else which was required for Azolla growth.At 37℃/29℃ (day/night) Azolla filiculoides grew poor than the corresponding Anabaena-tree Azolla grown in nitrogen-containing medium while Azolla filiculoides with the Anabaena from Azolla microfhylla (Af 135),a newly reconstituting Azolla,was able to grow much better than the Azolla with homologous Anabaena.However,Azolla microfhylla with the Anabaena from Azolla filiculoides grew and fixed nitrogen less than original one.the growth of Azlolla microfhylla was much better than Azolla filicwifos with the Anabaena from Azolla microfhylla.Therefore,the tolerance of Azolla to high temperature is determined by both host-fern and symbiont-Anabaena.
1992, 7(1): 36-40.
Abstract:
The investigation showed that the numbers of urease-producing microorganisms were identical with the urease activities in responding paddy soils whatever different seasons,soil type and the distribution of soil profile.The proportions of urease-producing populations among bacteria,actinomyces and fungi account for 32.5-85.5%,50-83% and 65-95% respectively.However,few of them showed high urease activity,and mostly were below 2.0 NH3-N mg/g· Protein·24hr · 37℃ To compare with the treatments of urea topdreasing and urea topdressing with azolla application,the urease activities in paddy soils of the treatments of deepplacement of urea ball fertilizer,and deepplacement of urea ball fertilizer with azolla were decreased.As a result,the urea efficiency was increased.
The investigation showed that the numbers of urease-producing microorganisms were identical with the urease activities in responding paddy soils whatever different seasons,soil type and the distribution of soil profile.The proportions of urease-producing populations among bacteria,actinomyces and fungi account for 32.5-85.5%,50-83% and 65-95% respectively.However,few of them showed high urease activity,and mostly were below 2.0 NH3-N mg/g· Protein·24hr · 37℃ To compare with the treatments of urea topdreasing and urea topdressing with azolla application,the urease activities in paddy soils of the treatments of deepplacement of urea ball fertilizer,and deepplacement of urea ball fertilizer with azolla were decreased.As a result,the urea efficiency was increased.
1992, 7(1): 41-48.
Abstract:
The result of investigation indicated that tolarance of Hybrid Azolla to salt was the best,followed by A.filiculoides and A.microphylla among three Azolla treated with different salt concentrations) during low salt concentration,Azolla were grown beneficially.The SOD activity rose higher with the increasing intensity of salt stress,but it started to reduce in certain salt concentrations (A.filiculodes and Hybrid Azolla in 0.6% NaCl,A.microphylla in 0.4% NaCl).The SOD gel electrophoresis showed the same results between SOD activity and straining intensity of enzyme bands.The ultrastructural changes of mesophyll cells showed that damage of membrance systems might be as s result of lipid reoxidation.It is suggested that SOD activity might be as an index of NaCl-resistance breeding.
The result of investigation indicated that tolarance of Hybrid Azolla to salt was the best,followed by A.filiculoides and A.microphylla among three Azolla treated with different salt concentrations) during low salt concentration,Azolla were grown beneficially.The SOD activity rose higher with the increasing intensity of salt stress,but it started to reduce in certain salt concentrations (A.filiculodes and Hybrid Azolla in 0.6% NaCl,A.microphylla in 0.4% NaCl).The SOD gel electrophoresis showed the same results between SOD activity and straining intensity of enzyme bands.The ultrastructural changes of mesophyll cells showed that damage of membrance systems might be as s result of lipid reoxidation.It is suggested that SOD activity might be as an index of NaCl-resistance breeding.
1992, 7(1): 49-54.
Abstract:
The average conent of availabe Zn in paddy fields of Ningde and Nanping Areas is 1.77 ppm.If the critical value of Zn deficiency for rice plant is 0.5ppm,about 30% area of paddy field in above two areas belongs to slightly Zn-deficient or potentialy Zn-deficient condition; about 10% belongs to seriously Zn-deficient condition.When the Zn content in paddy field is below 1.0ppm,the application of Zn fertilizer will receive a yield-increasing effect.In the case soil contains high Zn and available P,the Zn application will meet the requirement of high level of Zn.P nutritional balance and enhance the grain yield.The Zn application improve the protein and amino-acid contents in brown rice thus enhance the nutritional value of rice.It is suggested that Zn fertiliger will be applied in earlier stage of rice growth,and suitable amount is 15-30kg (ZnSo4,7H2O) /ha.The better Zn-applying methods are coating the roots of rice seedlings and foliage dressing.In severely Zn-deffecient soil,more high amount of Zn may be used as basal.
The average conent of availabe Zn in paddy fields of Ningde and Nanping Areas is 1.77 ppm.If the critical value of Zn deficiency for rice plant is 0.5ppm,about 30% area of paddy field in above two areas belongs to slightly Zn-deficient or potentialy Zn-deficient condition; about 10% belongs to seriously Zn-deficient condition.When the Zn content in paddy field is below 1.0ppm,the application of Zn fertilizer will receive a yield-increasing effect.In the case soil contains high Zn and available P,the Zn application will meet the requirement of high level of Zn.P nutritional balance and enhance the grain yield.The Zn application improve the protein and amino-acid contents in brown rice thus enhance the nutritional value of rice.It is suggested that Zn fertiliger will be applied in earlier stage of rice growth,and suitable amount is 15-30kg (ZnSo4,7H2O) /ha.The better Zn-applying methods are coating the roots of rice seedlings and foliage dressing.In severely Zn-deffecient soil,more high amount of Zn may be used as basal.
1992, 7(1): 55-58.
Abstract:
The study was based on the observation of the inflorescence development and sequence of flower differentiation in the Longan variaty of Dongbi.The result showed that the morphologic differentiation of flower bud in Longan could be divided into three stages,i.e.differentiation stages of main infloresence,lateral infloresence and flower organs,which were effected by the temperature in winter.In the basis of the initiation stage of the morphologic differentiation of flower buds in Longan beginning in the middle or last ten days of January,the relevant field practices for high and stable production including,cultivation of mother fruiting shoot,control of shoot and improvement of flower were also discussed.
The study was based on the observation of the inflorescence development and sequence of flower differentiation in the Longan variaty of Dongbi.The result showed that the morphologic differentiation of flower bud in Longan could be divided into three stages,i.e.differentiation stages of main infloresence,lateral infloresence and flower organs,which were effected by the temperature in winter.In the basis of the initiation stage of the morphologic differentiation of flower buds in Longan beginning in the middle or last ten days of January,the relevant field practices for high and stable production including,cultivation of mother fruiting shoot,control of shoot and improvement of flower were also discussed.
1992, 7(1): 59-65.
Abstract:
The effects of factors,such as substratum,hardening of natural lighting,seedling age,temperature and root in vivo (twing cutting) on the first transitional transplant in factory growing seedling of loquat were studied,and the effect of second trasitional cultivation on the survival rate in soil culture was discussed.The results show that:1.The suitable substrata for trasitional cultivation of tissue culture seedling (TCS) are peat soil and the materials consisted of 70%peat soil and 30%black soil.2.The relationship between hardening of natural lighting from 0-30 days and the survival rate of first transitional transplant is rectilinear relation.The TCS should be hardened by natural lighting for 30 days before first transitional transplant.3.The survival rate of first transitional transplant is high in aged seedling.The seedling age of 40-60 days is suitable for transplant to reduce the growing seedling period.4.The average air temperature during first transitional transplant of TCS is about 20℃,and the suitable range is 15-24℃.The TCS can be transplanted all the year round,but in consideration of advantages and disadvantages the transplant of TCS would be kept away from summer.5.The relationship of survival rate of first transitional transplant between the taken root in vitro and that in vivo is shown no difference.Judged by the reduction of production costs and the increase in benefit,the taken root in vivo is better than that in vitro.6.The product between the ultimate survival rate of second transitional cultivation of TCS and that of soil culture is remarkable higher than the survival rate of TCS which directly planted in soil after first transitional transplant.Thus,in the growing seedlings factory of loquat,the TCS from first transitional transplant would be planted in soil after second cultivation (two mounths).Finally,the possibility of TCS transition without damnification by open culture was discussed.
The effects of factors,such as substratum,hardening of natural lighting,seedling age,temperature and root in vivo (twing cutting) on the first transitional transplant in factory growing seedling of loquat were studied,and the effect of second trasitional cultivation on the survival rate in soil culture was discussed.The results show that:1.The suitable substrata for trasitional cultivation of tissue culture seedling (TCS) are peat soil and the materials consisted of 70%peat soil and 30%black soil.2.The relationship between hardening of natural lighting from 0-30 days and the survival rate of first transitional transplant is rectilinear relation.The TCS should be hardened by natural lighting for 30 days before first transitional transplant.3.The survival rate of first transitional transplant is high in aged seedling.The seedling age of 40-60 days is suitable for transplant to reduce the growing seedling period.4.The average air temperature during first transitional transplant of TCS is about 20℃,and the suitable range is 15-24℃.The TCS can be transplanted all the year round,but in consideration of advantages and disadvantages the transplant of TCS would be kept away from summer.5.The relationship of survival rate of first transitional transplant between the taken root in vitro and that in vivo is shown no difference.Judged by the reduction of production costs and the increase in benefit,the taken root in vivo is better than that in vitro.6.The product between the ultimate survival rate of second transitional cultivation of TCS and that of soil culture is remarkable higher than the survival rate of TCS which directly planted in soil after first transitional transplant.Thus,in the growing seedlings factory of loquat,the TCS from first transitional transplant would be planted in soil after second cultivation (two mounths).Finally,the possibility of TCS transition without damnification by open culture was discussed.
1992, 7(1): 66-71.
Abstract:
Anther culture of Asparagus was used M.S.as basic medium which added NAA and 6-BA to induce callus.Inductivity of callus could be raised by additional 2,4-D.The number of callu concerned not only cultivars but also individual plant in a cultivar.The complete plants could oh tain from the anther callus by differential cul-ture.Few haploids were observed while the chromosome of anther culture plants examined.
Anther culture of Asparagus was used M.S.as basic medium which added NAA and 6-BA to induce callus.Inductivity of callus could be raised by additional 2,4-D.The number of callu concerned not only cultivars but also individual plant in a cultivar.The complete plants could oh tain from the anther callus by differential cul-ture.Few haploids were observed while the chromosome of anther culture plants examined.
1992, 7(1): 72-77.
Abstract:
In vertical polyacrylamide slab gel electrophoresis analysis,the band patterns of esterase isozymes obtained from resistance differing sweet potato varieties to Pseudomonas solanaccarum are composed of many enzyme bands in proper ratio.The high,medium or susceptoble resistance varieties had their own band patters of esterase isozynes.Pattern 6 represents high resistance,pattern 4 medium and pattern 5,7,10 susceptible.The band patterns of esterase isozymes can be used as one of biochemical criteria in identification of the variety resistance,Being analyzed by ultraiolet ray screening,the ratio of OD readings in Pa area of peroxidase isozymes in diseased or health seedlings of sweet potato can also be used as one of biochemical criteria in identification of the variety resistance.
In vertical polyacrylamide slab gel electrophoresis analysis,the band patterns of esterase isozymes obtained from resistance differing sweet potato varieties to Pseudomonas solanaccarum are composed of many enzyme bands in proper ratio.The high,medium or susceptoble resistance varieties had their own band patters of esterase isozynes.Pattern 6 represents high resistance,pattern 4 medium and pattern 5,7,10 susceptible.The band patterns of esterase isozymes can be used as one of biochemical criteria in identification of the variety resistance,Being analyzed by ultraiolet ray screening,the ratio of OD readings in Pa area of peroxidase isozymes in diseased or health seedlings of sweet potato can also be used as one of biochemical criteria in identification of the variety resistance.
1992, 7(1): 78-83.
Abstract:
Six hybridoma cell lines secreting monoclonal antibodies (McAbs) against the pathogen of Pseudomonas solanacearum from sweet potato have been produced.The McAbs showed positive reactions to 7 sweet-potato-infected strains,and negative to other bacteria of Pseudomonas and genus else,and various bacteria infected seedings of sweet potato detected in the test.The ELISA liters of the culture supernatanls and the ascites antibodies were respectively 102-103 and 105-106.The sensitivity of antigen detected was 11.5μg/ml (protein concentration).The original characters of the hybridoma cell and culture supernatants antibodies could be kept over three years.Six antibodies showed positive to all of 3 pathogenicity types of Pseudomonas solanacearum from sweet potato in Fujian province.The inoculated samples could be detected out by the indirect ELISA at the rate of 100%.In 119 portions of symptonlatent seedlings collected from field cases,51 and 68 were respectively positive and negative.To compare with the antibody and inoculation detection,the result showed the sensitivity and specificity of antibody detection were more height than that of inoculation detection,and more rapid and handy.
Six hybridoma cell lines secreting monoclonal antibodies (McAbs) against the pathogen of Pseudomonas solanacearum from sweet potato have been produced.The McAbs showed positive reactions to 7 sweet-potato-infected strains,and negative to other bacteria of Pseudomonas and genus else,and various bacteria infected seedings of sweet potato detected in the test.The ELISA liters of the culture supernatanls and the ascites antibodies were respectively 102-103 and 105-106.The sensitivity of antigen detected was 11.5μg/ml (protein concentration).The original characters of the hybridoma cell and culture supernatants antibodies could be kept over three years.Six antibodies showed positive to all of 3 pathogenicity types of Pseudomonas solanacearum from sweet potato in Fujian province.The inoculated samples could be detected out by the indirect ELISA at the rate of 100%.In 119 portions of symptonlatent seedlings collected from field cases,51 and 68 were respectively positive and negative.To compare with the antibody and inoculation detection,the result showed the sensitivity and specificity of antibody detection were more height than that of inoculation detection,and more rapid and handy.
1992, 7(1): 84-87.
Abstract:
The present paper deals with new records of Carabidae in Fujian Province.Names of 37 species of insects belonging to 26 genera are given.Among them there are 10 species recorded newly in China,2 species,Carabus davidi and C.Coplolabrus lafossei are rare insects in the list of the national animal protection,morphological descriptions of these 2 species ore given in the paper.
The present paper deals with new records of Carabidae in Fujian Province.Names of 37 species of insects belonging to 26 genera are given.Among them there are 10 species recorded newly in China,2 species,Carabus davidi and C.Coplolabrus lafossei are rare insects in the list of the national animal protection,morphological descriptions of these 2 species ore given in the paper.
1992, 7(1): 88-91.
Abstract:
The popmlation dynamics of Raltus rattoides,a dominant rodent species in farmland in Fujian Province,Was investigated with the systematic catch rates/trap/ night.There were two peak and two valley periods of the catch rate in a year,i.e.April-May and August-September,and February-March and June-July,respectively; and there was significantly negative correlation between the catch rate and acreage proportion of crop appropriate for the damage of this rodent.It is suggested that the effective cavity rate is an available indication reflecting the population dymamics of farmland rodents.
The popmlation dynamics of Raltus rattoides,a dominant rodent species in farmland in Fujian Province,Was investigated with the systematic catch rates/trap/ night.There were two peak and two valley periods of the catch rate in a year,i.e.April-May and August-September,and February-March and June-July,respectively; and there was significantly negative correlation between the catch rate and acreage proportion of crop appropriate for the damage of this rodent.It is suggested that the effective cavity rate is an available indication reflecting the population dymamics of farmland rodents.
1992, 7(1): 92-96.
Abstract:
Changes of ureide,allantoin,allantoic acid content in leaves and nodules of adzuki bean (Vigna angularis) and effect of some exogenous compounds on the ureide content in seedlings were inverstigated.Diurnal variation of ureide content occured in leaves and nodules.Allantoic acid content was higher than that of allantoin in the nodules.Diurnal variation in ureide,allantoin,allantoic acid content in leaves displayed a peak at 16:00 which occured one hour later than that in the nodules.However,diurnal fluctuation patterns of allantoin content was similar to that of allantoic acid content in both the leaves and the nodules.Several compounds included in the growth medium were tested for their ability to affect the synthesis of allantoin,allantoic acid in germinating seedlings.Mn2+ showed no appreciable effect on the allantoinase activity and allantoin,allantioc acid synthesis,but Cu2+inhibited the allantoinase activity.However,KT (l-10ppm) and BA(l-25ppm) did not have any effect on the allantoinase activity.ZT(l-25ppm)had slight effect on the ureide content.ABA (lppm) repressed the germination but did not affect the ureide content.
Changes of ureide,allantoin,allantoic acid content in leaves and nodules of adzuki bean (Vigna angularis) and effect of some exogenous compounds on the ureide content in seedlings were inverstigated.Diurnal variation of ureide content occured in leaves and nodules.Allantoic acid content was higher than that of allantoin in the nodules.Diurnal variation in ureide,allantoin,allantoic acid content in leaves displayed a peak at 16:00 which occured one hour later than that in the nodules.However,diurnal fluctuation patterns of allantoin content was similar to that of allantoic acid content in both the leaves and the nodules.Several compounds included in the growth medium were tested for their ability to affect the synthesis of allantoin,allantoic acid in germinating seedlings.Mn2+ showed no appreciable effect on the allantoinase activity and allantoin,allantioc acid synthesis,but Cu2+inhibited the allantoinase activity.However,KT (l-10ppm) and BA(l-25ppm) did not have any effect on the allantoinase activity.ZT(l-25ppm)had slight effect on the ureide content.ABA (lppm) repressed the germination but did not affect the ureide content.
