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2010 Vol. 25, No. 5

Display Method:
Optimization of Edwardsiella tarda Fermentation to increase biomass
LI Ying, QIU Hong-duan, GONG Hui, XIE Hang, XU Bin-fu, LIU Xiao-dong
2010, 25(5): 541-547.
Abstract:
Using tryptone soya broth medium,the culture conditions of Edwardsiella tarda(ETY) were optimized by a single factor and an orthogonal test to enhance the cell concentration.The results showed that the optimized medium contained 3.0 g of glucose,10.0 g of tryptone,10.0 g of peptone,2.50 g of K2HPO4 and 5.0 g of NaCl per liter.And,the most favorable fermentation conditions were at 25℃,with an initial pH of 7.5,on a stirring speed of 200 rmin-1 and with the inoculating ratio of 8%.Compared with the control,the cell concentration of ETY could be improved from 5.200×109cfumL-1 to 1.575×1010cfumL-1,i.e.,3 times increase.At the same time,the cost of liquid medium was reduced by 41.36% compared to the conventional formulation.
Nutritional quality and composition in flesh of three species of groupers
LIN Jian-bin, CHEN Du-huan, ZHU Qing-guo, LIANG Ping, QIN Zhi-qing
2010, 25(5): 548-553.
Abstract:
The nutritional contents in the muscle of Epinephlus akaara,E.awoara and E.coioides were analyzed and evaluated.Protein content of the fresh muscle of E.awoara(21.76%) was higher than that of E.akaara(19.15%) or E.coioides(20.21%).The contents of total amino acids(TAA),essential amino acids(EAA) and flavoring amino acids(FAA) in dry weight fish flesh were 67.94%,27.35% and 26.30%,respectively,in E.akaara,72.25%,30.58% and 26.90%,respectively,in E.awoara,and 73.38%,30.77% and 27.53%,respectively,in E.coioides.The FAA to TAA ratio in E.akaara was the highest among the three groupers(38.71%).The amino acids score(AAS) and chemical score(CS) showed no significant difference among the three species.The EAA index of E.akaara was the highest(85.71) among the three groupers.The EPA+DHA contents in E.akaara and E.awoara were 20.4% and 20.5%,respectively,and they were higher than that in E.coioides.The results indicated that the three different species of groupers were nutritive and delicious seafood.Their nutritional values were similar,but the flavor of E.akaara and E.awoara appeared to be more desirable than that of E.coioides,based on their FAA contents.
Drug residues in fish raised on feed containing trace enrofloxacin and furazolidone
PAN Wei, CHEN You-sheng, HUANG Xing, LUO Qin, RAO Qiu-hua, LAN Jian-you, CHEN Ren-bi
2010, 25(5): 554-557.
Abstract:
Eels and tilapia were raised in aquarium for 6-month on the feed containing traces of enrofloxacin and furazolidone to determine the veterinary drug residues in the fish tissues.The result showed that,when the amount of enrofloxacin and furazolidone in feed was lower than 200 μgkg-1for tilapias and lower than 100 μgkg-1 for eels,the drug residues in the tissues of eviscerated fish was below the MRL standard for fish.Therefore,considered the feed is suitble for feeding the fish which is safe for human consumption.
Relationship between plant morphology and microspore development in fertility abortion of rice, SE21S
ZHANG Shui-jin, ZHAO Ming-fu, TU Shi-hang, XIE Hong-guang, ZHENG Jia-tuan, HUANG Ting-xu, YANG Ju-bao, HU Ru-ying, XIAO Cheng-he
2010, 25(5): 558-562.
Abstract:
Under natural conditions with long day and high temperature or short day and low temperature,the plant morphology,including leaf collar,ear length and days to heading,and the morphological changes of cells during fertility abortion were observed on the rice,SE21S.The relationship between the plant characteristics and microspore development was established.The results showed that(1) light and temperature were the significant climatic factors affecting the fertility when plant morphological characteristics were-1.0-4.5cm long on leaf collar,12.5-14.6cm long on young panicles,6.4-9.6 days before heading and in the single nucleus stage of the microspore development;and(2) the sterile pollen microspores appeared shrunk and irregular,while the fertile microspores smooth and regular.But during the stage from motrecyte to tetracyte,there was no significant difference in their cellular morphology observed in comparison with the normal rice(control).
Effect of sowing time and seedling density on yield of Teyou 103
GUO Fu-tai
2010, 25(5): 563-567.
Abstract:
Effect of sowing time and seedling density on yield of the rice,Teyou103,in early and late seasons was studied.The result showed that sowing time and seedling density affected mainly the spike number and grains per spike.Thus,with moderate fertilization,Teyou 103 could be sowed in mid-February for the early crop and in mid-July for the late crop at a seedling density of 650,000 per hm2 in southern Fujian for the highest possible yield.
Agronomic, yield and quality characteristics of a new dual-purpose peanut variety
ZHENG Xiang-li, YE Hua-lan, WANG Zheng-rong, XU Guo-zhong, WENG Bo-qi
2010, 25(5): 568-571.
Abstract:
To select a new dual-purpose strain,six strains of peanut were evaluated according to their agronomic characteristics,yield performances of both above-and under-ground materials,and nutritional value.The results showed that the plant height of TJ was greater than Quanhua No.7 at P0.05 level,the peanut yields of Shang G and TJ were 35.81% and 35.11%,respectively,higher than that of Quanhua No.10,and 26.12% and 25.47%,respectively,higher than that of Quanhua No.7 at P0.01 level.The peanut vine yield of TJ was 29.08% and 30.42% greater than that of Quanhua No.10 and Quanhua No.7,respectively,at P0.01 level.The peanut vine yield of Shang G was 14.29% and 15.46% greater than that of Quanhua No.10 and Quanhua No.7,respectively,at P 0.05 level.
Optimum harvest time for Liriope muscari(Decne.) Baily
HUANG Ying-zhen, CHEN Jing-ying, SU Hai-lan, HUANG Yu-ji
2010, 25(5): 572-575.
Abstract:
This study was conducted to determine the best harvest time for Liriope muscari(Decne.) Baily.Using the drying rate as an index,the yield of L.muscari(Decne.) Baily was determined every 15 days.The polysaccharide content was measured by using phenol sulfuric acid method.The result showed that the yield and total polysaccharide content in the tubers of L.muscari(Decne.) Baily were at their highest levels in mid-May.Therefore,it was recommended as the optimum harvest time.
Correlation between CCI and chlorophyll content in flue-cured tobacco
SHI Jin-lin, CHEN En-Bo, ZHANG Hui
2010, 25(5): 576-579.
Abstract:
Cultivar K326 for flue-cured tobacco was used to study the correlation between CCI and the chlorophyll content in the tobacco leaves.The result showed a significant positive correlation between the chlorophyll meter reading and the chlorophyll content.The correlation coefficients(R2) of CCI and chlorophyll content on different parts of a leaf were 0.8831 for the base,0.934 for the middle portion,and 0.8819 at the tip.Therefore,the middle part of a leaf would be the best choice for testing CCI for tobacco's chlorophyll content.This measurement method also had the advantages of being simple and convenient to use,as well as,non-intrusive to the sample.
Breeding and utilization of male sterile line of cauliflower
CHEN Wen-hui, FANG Shu-gui, ZHU Chao-hui, LIN He-fei, HUANG Jian-du
2010, 25(5): 580-583.
Abstract:
Some cauliflower cytoplasmic male sterile lines and their maintainer lines were obtained by crossing between three European cauliflower cytoplasmic male sterile materials with pure lines and inbred lines as recurrent parent.The sterile lines showed excellent general characteristics with high combining ability.All rates of sterile plant and sterility reached 100%.The lines were propagated by tissue culture.Three elite hybrids of the combinations crossed with the male sterile lines were obtained through field screening.
Isolation and activity of antibacterial compounds in Pleurotus sajor-caju Fermentation Broth
LIN Yong, ZENG Zhi-heng, ZHANG Di, ZHENG Yi, LIU Yan-ru, OUYANG Tong-jiao
2010, 25(5): 584-589.
Abstract:
The enriched fermentation broth of Pleurotus sajor-caju was extracted with ethyl acetate and followed by n-butanol.Several compounds were obtained from the extract by using thin layer chromatography and silica gel column chromatography.The isolated compounds were tested for their antibacterial activities.The active substances were further analyzed using GC-MS.Among them,Epsc1 and 2 and Npsc1 and 2 showed antibacterial activities,and Npsc1 had the strongest inhibitory effect on Staphylococeu aureus.
Propagation and renewal characteristics of loquat germplasm
CHEN Xiu-ping, PAN Shao-lin, HUANG Ai-ping, JIANG Ji-mou, DENG Chao-jun, ZHENG Shao-quan
2010, 25(5): 590-596.
Abstract:
Loquat growth characteristics were observed under same planting conditions using the root stock of Jiefangzhong.Grafting survival rate,seedling rate,graft growth volume and number of branches were recorded.The result showed that:(1) All growth characteristics were significantly different among the 255 germplasms with the coefficients of variance ranging from 15.07% to 28.45%.Very significant correlations existed between the grafting survival rate and the seedling rate(correlation coefficient=0.4221),growth volume of graft(correlation coefficient = 0.3299) and number of branches(correlation coefficient = 0.2837).(2) The growth volume of graft of the loquat germplasm from Yunnan was the largest,significantly larger than those from New Zealand,Jiangsu,Japan,Fujian and Hubei.The numbers of branches were significantly different among all samples,with those came from Sichuan to be the highest and those from Guangxi the lowest.(3) The graft growth volume of the wild loquat was very significantly larger than that of the cultivated loquat.And,(4) all growth characteristics of the polyploid loquat were very significantly poorer than the diploid loquat.The results provided a basis for the resource preservation and utilization of loquat germplasm.
Determination of Momordica's germplasm diversity by ISSR
KANG Jian-ban, ZHU Hai-sheng, LI Da-zhong, LI Yong-ping, WANG Wei-cai, WEN Qing-fang
2010, 25(5): 597-601.
Abstract:
The inter-simple sequence repeat(ISSR) method was applied to assess the genetic variations in 48 Momordica cultivars.From 96 ISSR primers 14 were employed on 48 samples.Out of 181 amplified bands,113 were found to be polymorphic(60.32%).The clustering analysis of ISSR results indicated that the genetic similarity indices were between 0.685 and 0.865.Thus,the genetic diversity,similarity and relationships among the cultivars were revealed providing a theoretical foundation for Momordica breeding in the future.
Factors affecting plantlet rooting from Dendrobium officinale tissue culture
CHEN Qing-qing, LAI Zhong-xiong, ZHU Jin-xiu
2010, 25(5): 602-605.
Abstract:
Effects of pH,light intensity and temperature on the growth and root formation of Dendrobium officinale cultivated in vitro were studied.Results showed that the height,rate of root formation and fresh weight of D.officinale were affected significantly by light intensity and temperature(P0.05);and that the root formation and fresh weight were significant affected by pH(P0.05),but not on the plantlet height.The optimal conditions for the growth and root formation of D.officinale were: pH 5.4 at 25℃ with the light intensity of 1500 lx.
On breeding Dendrobium candidum Wall.ex Lindl
LIN Jiang-bo, DAI Yi-min, ZOU Hui, WANG Wei-ying, WU Shui-jin
2010, 25(5): 606-609.
Abstract:
On an appropriate medium,Fujian's Dendrobium candidum Wall.ex Lindl was studied for its seed germination,plantlet strength,rooting and transplantation.The results indicated that the seeds germinated well and quickly on the medium consisting of MS +sucrose 30 gL-1 +agar 7 gL-1.To strengthen the plantlet and encourage root formation,the best formulation was found to be 1/4MS +sucrose 40gL-1 +potato extract 100 mgL-1+agar 7gL-1.The transplanting survival rate,average number of new buds and average number of new buds that were longer than 3 cm could best be obtained when the mixture of sawdust and peat in the ratio of 1:2 was used for the plantlet transplantation.
Shade tolerance of 5 varieties of Melastomataceae plants
YU Zhi-cheng, CHEN Zhen-dong, LIN Qiu-jin, Lin Xiu-xiang, SU Jin-qiang, ZHENG Tao
2010, 25(5): 610-613.
Abstract:
To investigate the shade tolerance of 5 varieties of Melastomataceae plants,3 levels of shading(i.e.,0,60 and 80%) were applied in this study.By determining the ratio of leaf area,the longest section distance,chlorophyll and soluble sugar content,the plant's tolerance to shading was determined.The results showed that Wufeng Melastoma intermedium Dunn and Changtai Melastoma intermedium Dunn exhibited a stronger shade tolerance,while Tibouchina aspera var.asperrima,Melastoma normale and Melastoma affine were less tolerant to shading.
Development of sawdust-based nursery medium for vegetable seedling
WANG Zai-xing, GUAN De-yi, LIU Zhi-qin, HE Shui-lin
2010, 25(5): 614-617.
Abstract:
Seedling nursery media were formulated on the basis of rotting theory and optimized by using Sawdust as its basic material.Air dried fowl manure and fermented pig manure were added for the organic nutrients.Effect of the mixes for cultivating pepper,cucumber and Chinese cabbage seedlings was studied.The resulting seedlings of the vegetables were healthy and similar to those nursed on the commercial grass carbon medium.However,the cost of the seedlings raised with the sawdust-based formulation was reduced by nearly 60%.A wide application to industrialize the vegetable seedling operation was seen possible with an additional benefit of conserving grass resource.
Resistance to crude R.solanacearum toxin of in vitro cultured peanut explants
YUAN Zong-sheng, LIU Fang, HU Fang-ping
2010, 25(5): 618-622.
Abstract:
Reaction of the peanut explants cultured in vitro to crude R.solanacearum toxin was studied.The results showed that the plant's resistance to the toxin could be determined by either smearing the toxin on the plant or mixing the toxin in the medium.It appeared that the percentage of callus formation on peanut leaf decreased as the toxin concentration increased,though not statistically significant among the samples.Based on the appearance of brown callus,the callus from the susceptible genotypes were more sensitive,showing greater browning,collapsing and other symptoms of damage,at earlier stage after treatment than the resistant plants.The in vitro observations seemed to parallel those in the field.
Effects of light pruning on arthropod population and community diversity in tea plantations
ZENG Ming-sen, LIU Feng-jing, WANG Ding-feng, WANG Qing-sen, WU Guang-yuan
2010, 25(5): 623-626.
Abstract:
In the winter,light pruning was found to reduce arthropods,which included spiders,larvae of Ectropis oblique hypulina Wehrli,Empoasca vitis Gothe,parasitoids and larvae of Homana coffearia Nietner,etc.All the abovementioned arthropods,except parasitoids,decreased in number significantly or very significantly when the cut tea branches were cleaned after pruning.In the summer,Empoasca vitis Gothe population decreased significantly after light pruning with clearing of the cut branches.Populations of spiders decreased significantly after light pruning even without clearing.On the other hand,the number of Myllocerinus aurolineatus Voss increased after light pruning.When the pruned branches were not cleared,the increase was obvious,but not significant.Populations of collembolans and arthropods both reached a higher,but not significant,level after light pruning.Their numbers decreased very significantly if the cut branches were removed.
Spatial distribution of Bactrocera(Zeugodacus) cucurbitae(Coquillett) larva on guava
YAN Zheng
2010, 25(5): 627-630.
Abstract:
Pattern of the spatial distribution of Bactrocera(Zeugodacus) cucurbitae(Coquillett) larva on guava was analyzed using various indices.The results showed that the pattern was aggregating and the colony was the basic distribution component.The aggregation might be caused by the behavior of B.cucurbitaelarvae,as well as,the environmental conditions.Based on the data collected,a model of sampling was established.Adequate fruit sampling size for at specific error level and density of larva is presented.The sequential sampling specimen number was calculated.
Effects of long-term fertilization on rice yield and contribution rate of basic soil productivity on the yellow paddy of southern China
WANG Fei, LIN Cheng, LI Qing-hua, HE Chun-mei, LI Yu, LIN Xin-jian
2010, 25(5): 631-635.
Abstract:
Effects of 27-year consecutive fertilization on rice yield and the contribution rate of basic soil productivity were studied on the southern yellow paddy.The result indicated that the application of chemical fertilizer(NPK),NPK and cattle manure(NPKM) or NPK and straw(NPKS) increased the crop yield by 74.9%,96.6% and 91.7%,respectively,as compared to non-fertilization(CK).The yields on land applied with NPKM and NPKS increased by 12.4% and 9.6%,respectively,as compared to NPK.The differences were all highly significant.But the difference between the yields from the rice yields applied with NPKM and those applied with NPKS were not significant.During the double-cropping period between 1983 and 2004,the year-on-year growth rate per 5-7 years showed a steady trend of increase by applying NPK,NPKM or NPKS.The contribution rates of basic soil productivity for the early and late rice showed a fluctuating but constant decreasing trend over the years.The mean contribution rates for the early and late rice were 47.1 and 60.0,respectively.The rice yields associated with different fertilization correlated significantly,or very significantly,with the basic soil productivity.
Database development on pests and their natural enemies for eight crop plants
YU De-yi, TANG Qing, LIN Jian-zhen, JI Jie, YAO Jin-ai, ZHANG Yan-xuan
2010, 25(5): 636-640.
Abstract:
The main purpose for establishing the database was to provide technical support for biological control on agricultural and forest pests.By using SQL Server 2005 database,ASP.NET web programming,B/S framework based on TCP/IP protocol,and three-layer structure,a database on pests and their natural enemies for 8 crop plants was developed.The database offered multiple inquiry methods,convenience in communication and interaction,as well as,excellent expandable functions.On the one hand,site operators could enter,modify or delete information,graphics or video in the database through the background management system.On the other hand,users could use the Internet to get their needed information through various retrieval methods or search engines.Interactive communication between users and the site could also be carried out through remote diagnosis system,remote video conference service and technical BBS.
Establishment of a multimedia information service system in Quanzhou under spark science & technology project 12396
LI Chao-can, HUANG An-ni, LIN Ming-li, HUANG Ai-min, QIU Mei-qin, ZHANG Yu-qi
2010, 25(5): 641-645.
Abstract:
Under the Spark Science Technology Project 12396,a multimedia information service system was established in Quanzhou.The entire system included telephone hotline,automatic voice prompt,Internet,video,web television,short message,QQ group,etc.for the functions,such as,organization and coordination,expert consultation,information retrieval,and public services.The Rural Science Technology Information Service Center and an agriculture advisory team were formed to provide remote training and consultation.
Design and establishment of Agricultural Sci-tec Information Network in Fujian
WANG Feng, WANG Jing-hui, ZHENG Hai-xin, LIU Shan-wen, HUANG Hui-zhen, BAO Wei, ZHANG Hai-jia
2010, 25(5): 646-650.
Abstract:
In order to provide information and a platform to foster development of modern agriculture in Fujian,the Agricultural Sci-tec Information Network was designed and established in Fujian.This article describes the formulation,objectives and contents of the system,as well as its future research,development,software,hardware,network structure,website design,resources development and security.
Performance evaluation on fundamental scientific research activities at non-profit research institutions
CHI Min-qing
2010, 25(5): 651-655.
Abstract:
This study aimed to establish a performance evaluation system on certain fundamental scientific research activities at the non-profit research institutions in Fujian.The performance management and evaluation would be incorporated into government's supervising operation.To resolve the complexity and uncertainty associated with the performance evaluation,a model consisting of evaluation indices were established.Furthermore,the AHP method was applied to analyze and determine the weight of individual indicators at different levels to facilitate the evaluation process.
Statistical analysis of published papers from Fujian Academy of Agricultural Sciences during 2000~2009
LIN Hai-qing, KE Wen-hui, WENG Zhi-hui
2010, 25(5): 656-661.
Abstract:
Based on the VIP and CNKI database,the published papers from Fujian Academy of Agricultural Sciences(FAAS) in 2000-2009 were analyzed by econometric methods.The index included total amounts of published paper,amounts of paper published in science and technology core journals,journal distribution,author distribution,cited frequency,downloaded frequency,etc.The index system reflected FAAS's research capacity and could be used to evaluate its academic standard by management department.