2021 Vol. 36, No. 4
Display Method:
2021, 36(4): 379-385.
doi: 10.19303/j.issn.1008-0384.2021.04.001
Abstract:
Objective An early season indica rice with excellent grain quality was bred through sexual hybridization between pedigrees and planted in regional trials for performance determination. Its genetic diversity and polymorphism were compared with the parents’. Method Hybridization of the new rice was carried out using Jiafuzhan as the female parent and Rongxian 1 as the male parent. The resulting cultivar was submitted to the regional trials in Fujian for new early rice varieties evaluation in 2017–2018 and simultaneously tested for production in 2018. Genetic diversity of the new cultivar and its parents based on the 48 pairs of identified SSR markers distributed in the 12 chromosomes of rice were compared. Result In the provincial 2017–2018 regional trial, the newly bred Quanzhen 12 delivered an average yield of 7 557.6 kg·hm−2, i.e., 1.82% higher than that of the reference, T78you 2155. However, its production yield in 2018 trial was 6.83% lower at 6 694.05 kg·hm−2. The grain quality of Quanzhen 12 was judged to be Grade 2 for edible rice according to the standard set forth by the Ministry of Agriculture. The variety was approved and released by Fujian Province Committee of Crop Variety Identification. The SSR makers showed the genetic similarity between Quanzhen 12 and Jiafuzhan to be 0.625, and between Quanzhen 12 and Rongxian 1 to be 0.375. Conclusion A new high-quality, short Quanzhen 12 was successfully bred. From the performances at the trials, it was considered suitable for early season planting in the province. Quanzhen 12 and Jiafuzhan appeared to have a close phylogenetic relationship.
2021, 36(4): 386-393.
doi: 10.19303/j.issn.1008-0384.2021.04.002
Abstract:
Objective A new sweet corn variety with high quality, yield, adaptability, and resistance to lodging and diseases was bred. Method The heterosis mode of “temperate germplasm × tropical germplasm” for crossing was applied to breed a super-sweet corn variety that would acquire the highly desirable traits of the parents. In 2013, the yellow super-sweet tropical corn, Mintianxi G73, bred by the Crop Research Institute of Fujian Academy of Agricultural Sciences was crossed with the temperate inbred line of white super-sweet variety, Mintianxi AS67, to result in Minshuangse No. 4. The new variety was submitted to the National Southeast Regional Trials on Fresh Corn by Science and Enterprise Consortium in the autumn session from 2016 to 2017. The combining abilities of Mintianxi G73 and Mintianxi AS67 were appraised in 2017, and the resulting yellow and white super-sweet corn hybrid was subsequently certified by the National Crop Variety Approval Committee in September 2018. Result On ear weight, Mintianxi AS67 exhibited the greatest general combining ability (GCA) and Mintianxi G73 moderate, while Mintianxi AS67 × Mintianxi G73 high on special combining ability (SCA). It indicated that the heterosis mode for hybridization was applicable for the intended purpose. In the 2-year national regional test, Mishuangse No. 4 displayed a 11 691.0 kg·hm−2 average yield on fresh ears, which was 4.3% higher than reference Yuetian No. 16, with 77.5% sampling points showing a yield increase. In the field tests, the hybrid displayed resistance to stem rot, moderate resistance to sheath blight, and strong resistance to lodging. It also scored 90.2 on comprehensive appearance and quality evaluaion, which exceeded the national high-grade standard for sweet corns and was significantly better than 85.0 did by Yuetian No. 16. The physiochemical analysis conducted on Minshuangse No. 4 by the College of Agriculture at Yangzhou University showed the 2-year averages on kernel residue at 10.2%, total soluble sugars at 20.2%, and reducing sugars at 8.3%. They were all superior to Yuetian No. 16. Conclusion Following the heterosis mode of “temperate germplasm × tropical germplasm” the resulting hybrid Minshuangse No. 4 upgraded the quality, yield, and resistance of the sweet corn. The variety was considered suitable for cultivation in southeast China.
2021, 36(4): 394-401.
doi: 10.19303/j.issn.1008-0384.2021.04.003
Abstract:
Objective To introduce premium large-seed broad bean varieties to Fujian, the agronomic characteristics of candidate germplasms were identified and evaluated against a local cultivar for cultivation in the province. Method Ten broad bean varieties from Qinghai and Jiangsu provinces were planted using Lingxiyicun as reference to compare the growth period, plant height, branch number, pod length, pod width, fresh pod yield, seed length, seed width, 100-seed weight, seed rate, and fresh seed yield of the plants for agronomic evaluation and crop yield. Result The growth period, fresh pod weight, pod width, 100-seed weight of fresh seed, number of primary branches and number of pod weight positively, but the plant height negatively, correlated with the fresh pod weight per mu. In the trial cultivation, the Qinghai variety was shorter on growth period, taller on plant height, fewer branches on a plant, lower yield, and less output value than the others. On the other hand, the Tongcanxian varieties from Jiangsu delivered superior performance and economic value. In particular, the pod length, pod width, fresh pod yield per mu, seed length, seed width, 100-seed weight, seed rate, and fresh seed yield of Tong-09-110-1 were the highest among all varieties under testing. Its crop yield of 100-dry-seed weight at 200.08g, seeding rate per mu at 50.53%, and fresh pod yield per mu at 1 200.58 kg were highly desirable as well. Conclusion Over all, Tong-09-110-1 was considered the most productive and suitable for large scale cultivation in Fujian.
2021, 36(4): 402-411.
doi: 10.19303/j.issn.1008-0384.2021.04.004
Abstract:
Objective Genetic information and resource of low-temp/draught resistant oil palms were studied with respect to the stress responses of polyamines synthase gene in the plants for breeding purposes. Method Bioinformatics of polyamines synthase genes in oil palm were gathered from the genome bank, and their expressions under abiotic stresses analyzed. Result Fifteen polyamines synthase genes were identified from the oil palm genome. There were between 318 and 720 amino acids in the genes with a molecular weight ranging from 36.00 kDa to 46.43 kDa and an isoelectric point between 4.78 and 6.31. The instability indices of the genes were found in between 29.26 and 48.73, the aliphatic indices in between 76.46 and 94.41, the gravy in between −0.315 and 0.082, and the exon number in between 1 and 11. They contained both specific conserved protein motifs and conserved domain. A phylogenetic analysis divided them into 3 families, i.e., EgADCs closely related to rice, EgACLs and EgSPDSs closely related to data palm, and EgSPMSs closely related to maize. Many cis-acting elements that response to phytohormone, stresses, and light were found in the promoters. The expressions of the polyamine synthase genes existed in different tissues of the plant and could be induced by either low temperature or draught stress. Specifically, EgSPDS1, EgSPDS2, EgSPMS1, EgADC1, EgADC2, EgACL5-2, EgACL5-3, EgSAMDC3-1, EgSAMDC4-1, EgSAMDC4-2, and EgACL5-1 were found to be upregulated by the imposed cold stress; while EgSPDS1, EgSPDS2, EgADC1, EgADC2, EgSAMDC4-1 EgSAMDC4-2, and EgACL5-1 by dehydration. Conclusion The polyamine synthase genes were significantly expressed in tissues of the oil palms that could be induced by either low temperature or draught stress. Those responded to the abiotic stresses were identified in this study.
2021, 36(4): 412-417.
doi: 10.19303/j.issn.1008-0384.2021.04.005
Abstract:
Objective Physiology and biochemistry associated with the flower bud differentiation of Narcissus tazetta var. chinensis suppressed by low temperature were studied. Method Three-year-old Chinese narcissus bulbs were stored under 15 ℃ or room temperature to compare the differences on the protective enzyme activity and soluble protein and carbohydrate contents in the scales. Results Under room temperature, the contents of superoxide anion (O2·−) and hydrogen peroxide (H2O2) in the scales rose initially followed by a decline and another rise; the activity of superoxide dismutase (SOD) remained high, peroxidase (POD) low, and catalase (CAT) ascended significantly in late stage; and, during the floret primordium developmental stage, the contents of sucrose and starch in scale declined, while α-amylase and soluble sugar stayed high. In contrast to room temperature, at 15 ℃ the O2·− and H2O2 in the scales increased but the SOD activity was not affected, while the POD and CAT activities in the stamen primordium significantly increased and the contents of soluble sugar, sucrose, and starch as well as the activity of α-amylase lowered at different sampling points. Conclusion The accumulation of carbohydrates, such as soluble sugars and starch, in narcissus scales was greatly affected by the low storage temperature at 15 ℃. It was crucial for the Chinese narcissus flower bud differentiation, particularly during the development of floret primordium.
2021, 36(4): 418-425.
doi: 10.19303/j.issn.1008-0384.2021.04.006
Abstract:
Objective The selected transcriptomes of Begonia semperflorens that can trigger the anthocyanin biosynthesis in the plant was used to clone the MYB transcription factor, and the cDNA databank applied to identify the potential interacting proteins associated with the biological process. Method Transcriptomes of B. semperflorens plant exposed to low temperature or intense light to induce the anthocyanin biosynthesis that turns the leaves red were screened for positive identification and cloning the full length cDNA of an MYB transcription factor, BsMYB62 (Gene ID: MT560845). Bioinformatics and potential interacting proteins of BsMYB62 were obtained with the designed primers and cloned cDNA. A pGBKT7-BsMYB62 bait vector was constructed and tested for toxicity and auto-activation. From the yeast two-hybrid library using the mating method, potential interacting proteins were named. Result The full length cDNA of BsMYB62 was determined to be 801 bp. The pGBKT7-BsMYB62 was found not toxic to the host yeast and capable of significantly inhibiting auto-activating in SD/-Trp-Leu-His-Ade medium including 45 mM of 3-AT. Nine potential interacting proteins were identified to include XTH9, LHB1B2, EBS7, PSI-F, UBE2, FDH, RBCS1A, FP6, and BCA. Conclusion It was speculated that BsMYB62 of B. semperflorens interacted with 9 proteins in response to the stress of low temperature or intense light.
2021, 36(4): 426-432.
doi: 10.19303/j.issn.1008-0384.2021.04.007
Abstract:
Objective Aromatic compounds and characteristics of Benifuji grapes grown in Tianjin were analyzed. Method Headspace solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) were used to extract, determine, and quantify by internal standards and standard curves on the aromatics in Benifuji grapes. Olfactory property of the grapes was characterized according to the odor activity value (OAV) and aroma profiling. Result Thirty-nine aromatic compounds in a combined concentration of 9 737.05 μg·L−1 were identified. Esters were the dominant substances followed by alcohols. Ethyl acetate, 1-hexanol, trans-2-hexene-1-ol, ethyl butyrate, and trans-2-hexene-1-al were the 5 major aromatics, totaling 9 461.79 μg·L−1 in concentration and accounting for 97.17% of the total relevant volatiles. Ethyl acetate concentration, which constituted 9 036.53 μg·L−1, was by far the greatest amount among all. It accounted for 98.53% of all esters and 92.81% of total aromatics. The OAV of the grapes was contributed mainly by 12 compounds, such as ethyl butyrate, ethyl-2-methylbutyrate, ethyl hexanoate, and ethyl isobutyrate. A visualized aroma profiling for the grapes showed characteristics of flowers, fruits, plants, fats, chemicals, and spices, with the fruity note the most predominant. Conclusion The aroma of Benifuji grapes grown in Tianjin was made of volatiles including esters, alcohols, and aldehydes, with the esters being the most predominant components in quantity.
2021, 36(4): 433-439.
doi: 10.19303/j.issn.1008-0384.2021.04.008
Abstract:
Objective Diversity and characteristics of Pyrus pyrifolia Nakai were studied according to the stone cell (SC) contents of the germplasms to establish a grading system to facilitate analyzing and classifying the resource. Methods SC content of the sand pear varieties was determined after freezing. A grading system was established based on the genetic diversity of the germplasms on SC content. Results The 480 germplasms under study showed a positive skewness statistic distribution on their SC contents, which ranged from 0.043% to 2.748% with an average of 0.706%, a variation coefficient (VC) of 61.11%, and a genetic diversity index (GDI) of 1.95. The average SC content of the local varieties was 0.886% with a VC of 48.82% and a GDI of 1.98, that of the foreign cultivars 0.440% with a VC of 48.79% and a GDI of 2.00, and that of the selected cultivars (lines) for breeding 0.400% with a VC of 47.02% and a GDI of 2.01. On genetic diversity, among the local cultivars the germplasms from Yunnan Province had the highest SC content of 1.239% and VC of 51.07%; those from Sichuan, the largest GDI of 1.90; those from Zhejiang, the lowest SC of 0.595%; and those from Guangdong, the smallest VC of 14.02% and GDI of 0.95. Of the selected cultivars for breeding, those from eastern China showed the lowest SC of 0.372%, while those from the central region the lowest VC of 43.41% and the highest GDI of 2.04. Conclusion The sand pear varieties investigated under this study exhibited a rich genetic diversity on their SC contents. Of all 480 germplasms, the local varieties had the highest SC content of 0.886% and the selected cultivars for breeding the greatest GDI of 2.01. To streamline the resource classification, 7 grades based on the SC content in the germplasms were established as SC＜0.1%, 0.1%≤SC＜0.2%, 0.2%≤SC＜0.4%, 0.4%≤SC＜0.7%, 0.7%≤SC＜1.1%, 1.1%≤SC＜1.6%, and SC≥1.6%. Their respective descriptive terms were scarce, low, medium low, moderate, moderately high, high, and extremely high on SC content.
2021, 36(4): 440-444.
doi: 10.19303/j.issn.1008-0384.2021.04.009
Abstract:
Objective Expression and distribution of the toll-like receptor (TLR) in porcine sperm were analyzed to facilitate the study on the related signaling pathway and antimicrobial properties. Method The mRNA expressions of TLR3, 7, 8, and 9 in pig sperm were detected by RT-PCR, and the distribution of TLR3 by immunofluorescence. Result The mRNA of TLR3, 7, 8, and 9 were all expressed in the sperm, and TLR3 mainly found in the posterior head and midpiece. Conclusion The expression of nucleic acid-sensing TLRs and the localizations of TLR3 in the porcine sperm revealed in this study gave clues of how the sperm might respond to attacks by virus and derivatives. The information paved the way for further investigation on the sperm TLR signaling pathway and pathogen recognition function.
2021, 36(4): 445-451.
doi: 10.19303/j.issn.1008-0384.2021.04.010
Abstract:
Objective Effects of feeding suspensions of chlorella culture with and/or without fermentation to broilers on the immunity of the birds were studied. Method A total of 480 healthy one-day-old male Liangfeng broilers were randomly divided into 4 groups of 10 replicates with 12 birds in each replicate. The broilers were all fed for 70 d with regular forage provided by Lanzhou Zhengda Co., Ltd. The drinking water provided for the 4 groups included water for control and 3 treatments by addition of 30 mL chlorella suspension (CS), 1 mL fermented chlorella suspension (FCS), or 30 mL combination of the two suspensions (CCS). Result The treatments significantly increased the thymus index in the broilers (P＜0.05) but no significant effect on the spleen index (P＞0.05). The improvements on the spleen and thymus tissue structures varied. The treatment broilers showed a significantly increased serum IgG (P＜0.05). Compared to control, the inflammation-promoting factors, such as TNF-α, IFN-γ, IL-1β, IL-2, and IL-6 in serum of the broilers were significantly reduced by the treatments (P＜0.05). For the anti-inflammatory functions, CCS significantly increased IL-4 (P＜0.05). Whereas FCS significantly decreased IL-1β and IL-6 (P＜0.05) and significantly increased IL-4 (P＜0.05). Conclusion The addition of NCS, FCS or CCS in drinking water for the broilers promoted the development of the immunity-related organs of the birds. In addition, significant increases on serum immunoglobulin, inhibition on inflammatory cytokines secretion, and enhancement on anti-inflammatory cytokines production were also found. It appeared that by incorporating the chlorella culture material, with and/or without subsequent fermentation, in the diet feeding broilers the immunity of the flock could be significantly improved.
2021, 36(4): 452-456.
doi: 10.19303/j.issn.1008-0384.2021.04.011
Abstract:
Objective Effect of silage time on nutrient contents of Cymbopogon citratus was studied. Method Changes on the nutrients in C. citratus during silage was monitored periodically to determine the optimal fermentation period for cattle feeding. Result After one month of silage, the protein and crude fiber in citronella increased significantly and peaked with the protein content rose from 5.48% to 6.64% and the crude fiber from 34.96% to 36.65%. In 2-4 months, the protein content became stable ranging from 6.32% to 6.39% before a significant decline, while the crude fiber remained at 34.23%-35.77% for 2-6 months. During the initial 4 months there was no significant changes on the calcium in citronella, but the content increased significantly in 5-6 month. For the first 5 months, the phosphorus content decreased initially followed by a slow incline to reach a peak in the 6th month. Up till 3 months of silage, the fermented citronella was judged to have a Level 1 sensory score with maximized contents on amino acids, crude fat, and ash as well as a constantly high crude protein content. Afterward, however, the sensory score dropped to Level 2 with decreasing crude protein, amino acid, crude ash, and crude fat contents with a deteriorated quality. Conclusion It appeared that 1-3 month of silage would allow citronella to reach the highest nutrient level and prolonged storage could considerably ill-affect the forage quality.
2021, 36(4): 457-463.
doi: 10.19303/j.issn.1008-0384.2021.04.012
Abstract:
Objective Pathogen that caused the leaf spot disease on Chimonanthus salicifolius was isolated and classified, and its biological characteristics determined. Method Specimens of C. salicifolius plants infected by leaf spot disease at Shouning county in Fujian were collected for in vitro tissue culture in the laboratory. Morphological and molecular examinations were performed on the isolates. Biological properties and culture conditions including temperature, pH, light, carbon source, nitrogen source, and lethal temperature were scrutinized for the pathogenic identification and characterization. Result Based on the morphology as well as the rDNA ITS and TUB sequences, the pathogen responsible for the disease was identified to be Ascomycetes Pleosporales Epicoccum sorghinum. It could grow at 5-30 ℃ with an optimum temperature at 25 ℃ and pH ranging from 5 to 9 in media of 11 various carbon sources including sucrose and 8 different nitrogen source including peptone under no specific light/dark conditions. The lethal temperature of the fungal mycelia was 52 ℃. Conclusion E. sorghinum was identified to be the major pathogen that caused the leaf spot disease on C. salicifolius in Shouning county. It had strong ability to grow on, resist to, and survive under adverse conditions.
2021, 36(4): 464-472.
doi: 10.19303/j.issn.1008-0384.2021.04.013
Abstract:
Objective Commercial soil passivating agents were compared for their effects on the heavy metal uptakes by leafy vegetables in the field of acidic soil in Fujian. Method A farm in Pingnan county contaminated with Pb, Cd, and Cr was selected to conduct the tests for determining the effects of 8 passivating agents on yield and heavy metal content of Pakchoi (Brassica rapa subsp. chinensis) in the 1st season and Chinese cabbage (B. rapa subsp. pekinensis) in the 2nd season grown on the lot. pH and DTPA-extractable in the soil were measured to correlate them with the migration of the heavy metals to the vegetables. Result (1) Application of either the heavy metal passivator, OSA, or Tebbe Calcium Conditioner significantly increased the yield of Pakchoi by up to 84.2% and Chinese cabbage by up to 65.4% over control. (2) Yonker Soil Conditioner or OSA produced the most significant rises on the soil pH to 5.25 on the lot during the 1st season when Pakchoi were planted and 4.76 in the subsequent season for growing Chinese cabbages. (3) Sea Crown Soil Conditioner significantly reduced DTPA-extracted Cd in the soil of the Pakchoi lot by 31.0% and of the Chinese cabbage lot by 25.4% over control. Among the treatments, Yonker and Sea Crown rendered the highest reductions on the DTPA-extracted Pb, while OSA and Kangyuan Soil Conditioner on DTPA-extracted Cr in both vegetable lots. (4) Of all the agents applied, the best results by comparing to control were found with OSA, which significantly decreased Pb in Pakchoi by 41.5% and in Chinese cabbages by 46.0%, with OSA or Yonker, which significantly decreased Cd in Pakchoi by 46.0% and in Chinese cabbages by 34.6%, and with Ten-thousand-mu Organic Fertilizer or Yonker, which significantly reduced Cr in Pakchoi by 73.2% and in Chinese cabbages by 60.6%. In the soil, OSA, Tebbe, or Yonker also effectively reduced the Cd at the Pakchoi lot to be within the national safety standard limit. (5) Positive correlations between the Pb, Cd, and Cr contents in vegetables and the DTPA-extractable in soil (P＜0.01), as well as a negative correction between the heavy metals in vegetables and soil pH (P＜0.01). It suggested that the passivating agents raised the pH of the acidic soil and effectively lowered and mitigated the heavy metals migration from the soil resulting in significant reduction on Pb/Cd/Cr in Pakchoi and Chinese cabbages grown on the land. Conclusion It appeared that the tested passivator OSA and conditioner Yonker were effective in remediating Pb/Cd/Cr-polluted acidic soils for vegetable farming.
2021, 36(4): 473-479.
doi: 10.19303/j.issn.1008-0384.2021.04.014
Abstract:
Objective Composition and diversity of rhizosphere bacterial community in response to straw-mulching and soil conditioning on land of maize field were studied. Methods Soil at the maize growing fields was treated with either no chemical fertilizer (CK), a single chemical fertilizer (T1), straw-mulching and chemical fertilization (T2), or a soil conditioner plus chemical fertilization (T3). The second-generation technology of Illumina Miseq high-throughput sequencing was used based on the bacterial 16S rRNA genes in V3-V4 region to determine the changes of diversity, richness, composition, and structure of bacterial community in the rhizosphere soil under treatment. Results The dominant bacteria, which showed their relative abundance greater than 10%, in the rhizosphere bacterial community were 4,183 genera of Proteobacteria, Chloroflexi, and Actinobacteria. The ACE and Chao 1 richness indices of the community under T1 were 17.65% and 17.88%, respectively, lower, while those under T3, 14.52% and 14%, respectively, higher than those under CK. At genera level, the stratified cluster diagram of community structure under T3 differed from those under CK, T1, and T2. Conclusion T3 significantly affected the structure of the bacteria community and more so than did the other treatments. Among all treatments, T1 lowered, while T3 enhanced, the richness of the community over CK. And the added soil conditioner raised the pH of the acidic soil rendering the greatest effect on the rhizosphere bacterial community in maize field.
2021, 36(4): 480-489.
doi: 10.19303/j.issn.1008-0384.2021.04.015
Abstract:
Objective Microorganisms that can efficiently decompose tea dregs at high temperature were screened for potential waste treatment application. Method A high temperature special habitat separation method was employed to isolate cellulase and protease-producing bacteria on tea dregs. Morphological, physiochemical, and growth characteristics of selected strains were examined prior to 16S rDNA cloning for sequencing. Gene sequences of the candidates, along with gyrB, were subjected to phylogenetic analysis for species identification. Decomposing capacity of the isolated strains on tea dregs was determined for final selection. Results Five thermophiles exhibiting the desired properties were isolated. Among them, Fb showed the highest enzymatic activities and was found to be a strain of Bacillus velezensis. It was further characterized with a high temperature tolerance up to 55 ℃ and the optimal culture conditions of 42-45 ℃, pH 5.0-7.0, 16 h incubation, 1.0-6.0% salinity, 30 mL 250 min−1 filling volume, and 120 rpm shaker speed. After Fb inoculation and incubation for 7 d, the crude protein in the resulting tea dregs significantly increased by 14.88% (P＜0.05), the contents of 14 amino acids, except cystine, methionine, and histidine, significantly increased (P＜0.05), the total amino acids increased by 5.98%, the crude fiber decreased by 9.69%, and the neutral detergent fiber, acid detergent fiber, and lignin decreased by 10.72%, 4.47%, and 11.37%, respectively (P＜0.05). Conclusion For the first time, B. velezensis Fb was identified to be capable of efficiently decomposing tea dregs with a significantly improved nutritional profile on the substrate. It could conceivably become a bioagent for treating the waste material.
2021, 36(4): 490-500.
doi: 10.19303/j.issn.1008-0384.2021.04.016
Abstract:
Objective Optimize the microbial fermentation and deodorization process of Gracilaria lemaneiformis (GL), and provide certain technical support for the deep processing and utilization of GL. Method Through the fuzzy comprehensive evaluation of sensory, on the basis of screening out the combination of biological deodorizing microorganisms, using the Box-Behnken response surface method o logy to optimize the added amount of malic acidandsugar, inoculation amount, time of fermentation, fermentation temperature factor parameters. Analyze the changes of volatile flavor compounds in GL before and after fermentation by HS-SPME-GC/MS and other techniques. Results The optimal deodorization process is: the combination of deodorizing microbial strains is 1∶1 Saccharomyces cerevisia JJ4 and Lactobacillus paracasei subsp. Paracasei RP38;sugar added 2.0%, malic acid added 0.2%, inoculation amount 5% each, fermentation time 68 h, fermentation temperature 29℃.After fermentation and deodorization, cyclooctanol, 1-methylcycloheptanol, octanal, nonanal, 2-nonenal, (E)-which have fatty, fishy and fishy odors disappear, the content of 3-octanoneis significantly reduced, added aromatic phenylethyl Alcohol, fruity hexanoic acid, ethyl ester, wine scented octanoic acid, ethyl ester, sweet scented hexanoic acid, 2-phenylethyl ester, coconut scented nonanoic acid, ethyl ester. Conclusion The combination of JJ4 and RP38 is used to ferment GL to remove the smelly substances such as alcohol and aldehydes, and increase the flavor substances such asphenylethylalcohol and esters.Andit has obvious deodorant and fragrance effect on GL.
