Construction and Identification of Full-length cDNA Library of Agaricus bisporus As2796

CHEN Mei-yuan

doi:10.19303/j.issn.1008-0384.2012.11.012

Volume 27 Issue 11

Nov. 2012

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CHEN Mei-yuan. Construction and Identification of Full-length cDNA Library of Agaricus bisporus As2796[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1201-1204. doi: 10.19303/j.issn.1008-0384.2012.11.012

Citation:

CHEN Mei-yuan. Construction and Identification of Full-length cDNA Library of Agaricus bisporus As2796[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1201-1204. doi: 10.19303/j.issn.1008-0384.2012.11.012

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Construction and Identification of Full-length cDNA Library of Agaricus bisporus As2796

doi: 10.19303/j.issn.1008-0384.2012.11.012

CHEN Mei-yuan

Institute of Edible Fungi,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350014,China

Received Date: 2012-10-11
Publish Date: 2012-11-18

Abstract

Abstract

A.bisporus As2796 is the main cultivar in China.For easily cloning the related target genes,the full-length cDNA library of As2796 was constructed.The library had a content of 3.3×106 cfu·mL-1,an insertion range of about 0.7-4.0 kb,an average cDNA length of 1.5 kb,a recombination efficience of about 82%,and full-length ratio of about 35%.The coding sequences(CDS) of 5 target genes were got from the library by PCR amplification,which proved the validity of the library for amplifying full-length cDNA or CDS of A.bisporus genes in need.
- edible fungus,
- gene library,
- coding sequence(CDS)

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References(15)

References

[1]	卢圣栋.现代分子生物学实验技术[M].第2版.中国协和医科大学出版社,北京:1999:295.
[2]	沈倍奋.分子文库[M].北京:科学出版社,2001:1-190.
[3]	ELLIOTT T J.Genetic Engineering and Mushrooms[J].Mushroom J,1988,184:528-529.
[4]	陈美元,王泽生,廖剑华,等.双孢蘑菇A41基因组文库的构建及筛选[J].食用菌,2001,23(S):84-86.
[5]	王泽生,陈美元,廖剑华,等.双孢蘑菇部分cDNA文库的构建及筛选[J].菌物学报,2004,(23):63-65.
[6]	NOEL T,LABARE′RE J.Isolation of DNA from Agrocybeaegerita for the construction of a genomic library in Escherichiacoli[J].Mushroom Sci,1987,12:187-201.
[7]	吕作舟,马爱民.平菇基因组文库的构建[J].中国食用菌,1992,11(6):15-16.
[8]	吕作舟,马爱民.平菇基因组文库的构建[J].中国食用菌,1993,12(1):13-14.
[9]	陈明杰,赵绍惠,张树庭.草菇基因文库的构建[J].食用菌学报,1994,1(2):16-20.
[10]	WANG Z S,LIAO J H,LI F G,et al.Studies on breedinghybrid strain As2796of Agaricus bisporus for canning in China[J].Mushroom Science,1995,14(1):71-79.
[11]	FERMADA SILVEIRA BUENO,ALINE ROMUC,MARKWACH,et al.Variability in commercial and wild isolates ofAgaricus species in Brazil[J].Mushroom Science,2008,17:135-148.
[12]	王泽生,廖剑华,陈美元.我国双孢蘑菇育种研究与产业发展[J].食用菌学报,2010,(S):19-20.
[13]	陈美元,王泽生,李洪荣,等.双孢蘑菇分解基质能力退化的DDRT-PCR分析[J].菌物学报,2010,29(5):707-712.
[14]	J.萨姆布鲁克,E.F.弗里奇,T.曼尼阿蒂斯著分子克隆实验指南[M].第2版.金冬雁,黎孟枫,译.北京:科学出版社,1992:160-161.
[15]	陈美元,廖剑华,王波,等.中国野生蘑菇属90个菌株遗传多样性的DNA指纹分析[M].食用菌学报,2009,16(1):11-16.