Minimum Dosage of Vibrio vulnifiticus OMP-ISCOMs for Vaccinating Anguilla anguilla

XU Bin-fu; GONG Hui; CHEN Xiu-jin; TIAN Ding; CHEN Qiang; CHEN Yong-cong; LIN Tian-long

doi:10.19303/j.issn.1008-0384.2012.12.002

Volume 27 Issue 12

Dec. 2012

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XU Bin-fu, GONG Hui, CHEN Xiu-jin, TIAN Ding, CHEN Qiang, CHEN Yong-cong, LIN Tian-long. Minimum Dosage of Vibrio vulnifiticus OMP-ISCOMs for Vaccinating Anguilla anguilla[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1280-1282. doi: 10.19303/j.issn.1008-0384.2012.12.002

Citation:

XU Bin-fu, GONG Hui, CHEN Xiu-jin, TIAN Ding, CHEN Qiang, CHEN Yong-cong, LIN Tian-long. Minimum Dosage of Vibrio vulnifiticus OMP-ISCOMs for Vaccinating Anguilla anguilla[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1280-1282. doi: 10.19303/j.issn.1008-0384.2012.12.002

Citation:

XU Bin-fu, GONG Hui, CHEN Xiu-jin, TIAN Ding, CHEN Qiang, CHEN Yong-cong, LIN Tian-long. Minimum Dosage of Vibrio vulnifiticus OMP-ISCOMs for Vaccinating Anguilla anguilla[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1280-1282. doi: 10.19303/j.issn.1008-0384.2012.12.002

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Minimum Dosage of Vibrio vulnifiticus OMP-ISCOMs for Vaccinating Anguilla anguilla

doi: 10.19303/j.issn.1008-0384.2012.12.002

1. Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;
2. Fujian Center for Disease Control and Prevention,Fuzhou,Fujian 350001,China

Received Date: 2012-07-30
Publish Date: 2012-12-18

Abstract

Abstract

Antigenicity of the immune stimulating complexes in the outer membrane protein(OMP-ISCOMs) of Vibrio vulnifiticus was studied by injecting the vaccine into the European eels(Anguilla anguilla).The minimum dosage required for effective vaccination was determined by serology and immune protection test.The immune response was dosage dependent.Thirty days after the eels were challenged with the V.vulnificus FJ03-X2 strains at 200,100,50 and 25 μg·mL-1 to result in 100%,87.5%,75% and 50% of protection,respectively;and the titers of serum antibody of 1∶6400,1∶3200,1∶800 and 1∶200 in turn,respectively.It appeared that the minimum dose of the OMP-ISCOMs of Vibrio vulnifiticus vaccine was 0.59 μg·g-1.
- Vibrio vulnifiticus,
- OMP-ISCOMs,
- minimum immune dose,
- Anguilla anguilla

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References(8)

References

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