Transgenic Rice with mgfp4 Gene Mediated by Agrobacterium tumefaciens

ZHOU Shu-fen

doi:10.19303/j.issn.1008-0384.2012.12.004

Volume 27 Issue 12

Dec. 2012

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ZHOU Shu-fen. Transgenic Rice with mgfp4 Gene Mediated by Agrobacterium tumefaciens[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1287-1291. doi: 10.19303/j.issn.1008-0384.2012.12.004

Citation:

ZHOU Shu-fen. Transgenic Rice with mgfp4 Gene Mediated by Agrobacterium tumefaciens[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1287-1291. doi: 10.19303/j.issn.1008-0384.2012.12.004

ZHOU Shu-fen. Transgenic Rice with mgfp4 Gene Mediated by Agrobacterium tumefaciens[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1287-1291. doi: 10.19303/j.issn.1008-0384.2012.12.004

Citation:

ZHOU Shu-fen. Transgenic Rice with mgfp4 Gene Mediated by Agrobacterium tumefaciens[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1287-1291. doi: 10.19303/j.issn.1008-0384.2012.12.004

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Transgenic Rice with mgfp4 Gene Mediated by Agrobacterium tumefaciens

doi: 10.19303/j.issn.1008-0384.2012.12.004

ZHOU Shu-fen

1. Key Laboratory of Agricultural Genetic Engineering of Fujian Province,Institute of Biotechnology,Fuzhou, Fujian 350003,China;
2. Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

Received Date: 2012-10-05
Publish Date: 2012-12-18

Abstract

Abstract

Plant expression vector,pCAGFPHyg,carrying mgfp4 gene was introduced into rice Minghui 86(Indica Sativa L.) mediated by Agrobacterium tumefaciens.The transgenic rice containing 101 independent clones was obtained.PCR and the southern blot analysis ensured that the exogenous genes were indeed integrated into the rice genome.The result of T1 segregation analysis from T0 rice seeds indicated that most of the transgenic plants were inserted by a single T-DNA.It was confirmed by the fluorescence microscopic and RT-PCR detections that the mgfp4 gene was expressed in the transgenic rice,although no acceptable result of green fluorescence was secured.
- Rice,
- green fluorescent protein gene,
- Agrobacterium-mediated,
- genetic transformation

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References(20)

References

[1]	汪波,彭定祥,孙珍夏,等.根癌农杆菌介导苎麻转绿色荧光蛋白(GFP)基因植株再生[J].作物学报,2007,3(10):1606-1610.
[2]	周盛梅,孟凡国,黄大年,等.绿色荧光蛋白及其应用[J].生物工程进展,1999,19(2):56-59.
[3]	MAREK H,SLAVOMR R,VLADISLAV C.Greenfluorescent protein as a vital marker for non-destructivedetection of transformation events in transgenic plants[J].PlantCell Tiss Org Cult,2006,86(3):303-318.
[4]	HU W,CHENG C.Expression of Aequorea fluorescentprotein in plant cells[J].FEBS Lett,1995,369:331-334.
[5]	徐晓晖,林佳楠,言思敏,等.绿色荧光蛋及其突变体的特征与应用[J].安徽农业科学,2011,39(7):3848-3850.
[6]	STEWART CN JR.The utility of green fluorescent protein intransgenic plants[J].Plant Cell Rep,2001,20(5):376-382.
[7]	苏军,胡昌泉,翟红利,等.农杆菌介导籼稻明恢86高效稳定转化体系的建立[J].福建农业学报,2003,18(4):209-213.
[8]	MURRY M G,THOMPSON W F.Rapid isolation of highmolecular weight plant DNA[J].Nucl Acid Res,1980,8(19):4321-4326.
[9]	CHIU W,NIWA Y,ZENG W,et al.Engineered GFP as avital reporter in plants[J].Curr Biol,1996,6(3):325-330.
[10]	HUANG Y,XU X,LI B.Improved green fluorescent proteinas a fast reporter of gene expression in plant cells[J].Biotechnology Techniques,1997,11(2):133-136.
[11]	许新萍,黄粤,卫剑文,等.绿色荧光蛋白基因在水稻细胞中的高效表达[J].植物学报,1998,40(1):91-94.
[12]	TIAN L,SEQUIN A,CHAREST P J.Expression of thegreen fluorescent protein gene in conifer tissue[J].Plant CellReports,1997,16(5):267-271.
[13]	王泽宙,邱全胜.绿色荧光蛋白基因mgfp4在水稻愈伤组织中的瞬时表达[J].北京师范大学学报,2000,36(3):385-389.
[14]	GEEST A H M,PETOLINO J F.Expression of a modifiedgreen fluorescent protein gene in transgenic maize plants andprogeny[J].Plant Cell Reports,1998,17(10):760-764.
[15]	PONAPPA T,BRZOZOWSKI A E,FINER J J.Transientexpression and stable transformation of soybeanusing jellyfishgreen fluorescent protein[J].Plant Cell Reports,1999,19(1):6-12.
[16]	PANG S Z,DEBOER D L,WAN Y,et al.An improvedgreen fluorescent protein gene as a vital marker in plants[J].Plant Physiol,1996,112:893-990.
[17]	陈大桦,叶和春,李国凤,等.绿色荧光蛋白基因在青蒿转基因芽中的表达[J].植物学报,1999,(5):490-493.
[18]	HARPER B K,MABON S A,LEFFEL S M,et al.Greenfluorescent protein as a marker forexpression of a second genein transgenic plants[J].Nat Biotechnol,1999,17:1125-1129.
[19]	LEFFEL S M,MABON S A,STEWART CN JR.Applications of green fluorescent protein in plants[J].Biotechniques,1997,23:912-918.
[20]	STEWART CN J R.Monitoring transgenic plants using invivo markers[J].Nat Biotechnol,1996,14(6):682.