Optimization for Regeneration System of Embryonic Tips of Vegetable Soybean

HU Run-fang; ZHANG Yu-mei; ZHANG Guang-qing; LIN Guo-qiang

doi:10.19303/j.issn.1008-0384.2013.01.013

Volume 28 Issue 1

Jan. 2013

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HU Run-fang, ZHANG Yu-mei, ZHANG Guang-qing, LIN Guo-qiang. Optimization for Regeneration System of Embryonic Tips of Vegetable Soybean[J]. Fujian Journal of Agricultural Sciences, 2013, 28(1): 51-54. doi: 10.19303/j.issn.1008-0384.2013.01.013

Citation:

HU Run-fang, ZHANG Yu-mei, ZHANG Guang-qing, LIN Guo-qiang. Optimization for Regeneration System of Embryonic Tips of Vegetable Soybean[J]. Fujian Journal of Agricultural Sciences, 2013, 28(1): 51-54. doi: 10.19303/j.issn.1008-0384.2013.01.013

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Optimization for Regeneration System of Embryonic Tips of Vegetable Soybean

doi: 10.19303/j.issn.1008-0384.2013.01.013

Crop Sciences Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China

Received Date: 2012-06-29
Publish Date: 2013-01-18

Abstract

Abstract

Embryonic tips from 'Mindou No.5' were used in testing the effect of NaClO on sterilization and homomycin B on screening stress for inducing of adventitious buds and root growing.The result showed that the optimal effect on sterilization was 10% NaClO for 10 min;the screening stress of hygromycin B was 7 mg·L-1 at elongation stage of buds from embryonic tips.Comparing culture mediums,the rooting culture medium presented better in root growing without 6-BA,the medium consisting of 1/2 MS salt+1/2 B5 organic matter +3% sucrose+0.56% agar+1.0 mg·L-1 IBA,pH 5.8.
- vegetable soybean,
- embryonic tips,
- regeneration,
- homomycin B

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References(12)

References

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