EDTA-Na_2 Extraction of Agarose with Response Surface Optimization

LI Long; SU Yong-chang; WU Cheng-ye

doi:10.19303/j.issn.1008-0384.2013.02.014

Volume 28 Issue 2

Feb. 2013

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LI Long, SU Yong-chang, WU Cheng-ye. EDTA-Na_2 Extraction of Agarose with Response Surface Optimization[J]. Fujian Journal of Agricultural Sciences, 2013, 28(2): 158-165. doi: 10.19303/j.issn.1008-0384.2013.02.014

Citation:

LI Long, SU Yong-chang, WU Cheng-ye. EDTA-Na_2 Extraction of Agarose with Response Surface Optimization[J]. Fujian Journal of Agricultural Sciences, 2013, 28(2): 158-165. doi: 10.19303/j.issn.1008-0384.2013.02.014

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EDTA-Na_2 Extraction of Agarose with Response Surface Optimization

doi: 10.19303/j.issn.1008-0384.2013.02.014

1. College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;
2. Fisheries Research Institute of Fujian,Xiamen,Fujian 361013,China

Received Date: 2012-12-17
Publish Date: 2013-02-18

Abstract

Abstract

This paper studied the technique of agarose extraction with EDTA-Na2 method.The main factors discussed were: EDTA-Na2 concentration,extraction temperature and extraction time on influencing in sulfate radical content and gel strength.Using the response surface method to optimize the extraction process,the best parameters were EDTA-Na2 concentration 4.5 g·L-1,extraction temperature 65℃ and extracting time 3 h.With the optimized parameters,the sulfate radical content and gel strength of agarose extracted were 0.48% and 1 073.09 g·cm-2,respectively.The optimal extraction parameters were verified by repetitive tests with relative error <1.0%.It is suggested that EDTA-Na2 method is accurate and repeatable in using for agarose extraction.
- Response surface method,
- EDTA-Na2 method,
- agarose

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References(17)

References

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