Effects of BSA-ISCOMs on Immunity Response and Disease Resistance of Pseudosciaena crocea

CHEN Xiu-xia; HUANG Dian-sheng; KE Ling; XU Bin-fu; FANG Dong-lan; GONG Hui

doi:10.19303/j.issn.1008-0384.2013.03.002

Volume 28 Issue 3

Mar. 2013

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CHEN Xiu-xia, HUANG Dian-sheng, KE Ling, XU Bin-fu, FANG Dong-lan, GONG Hui. Effects of BSA-ISCOMs on Immunity Response and Disease Resistance of Pseudosciaena crocea[J]. Fujian Journal of Agricultural Sciences, 2013, 28(3): 201-205. doi: 10.19303/j.issn.1008-0384.2013.03.002

Citation:

CHEN Xiu-xia, HUANG Dian-sheng, KE Ling, XU Bin-fu, FANG Dong-lan, GONG Hui. Effects of BSA-ISCOMs on Immunity Response and Disease Resistance of Pseudosciaena crocea[J]. Fujian Journal of Agricultural Sciences, 2013, 28(3): 201-205. doi: 10.19303/j.issn.1008-0384.2013.03.002

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Effects of BSA-ISCOMs on Immunity Response and Disease Resistance of Pseudosciaena crocea

doi: 10.19303/j.issn.1008-0384.2013.03.002

Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

Received Date: 2012-12-27
Publish Date: 2013-03-18

Abstract

Abstract

Immune stimulating complex(ISCOMs) is an efficient mucosal delivery system which can induce non-specific immune response.Bovine serum albumin(BSA) was incorporated into ISCOMs(BSA-ISCOMs).Pseudosciaena crocea took diets containing BSA-ISCOMs for 10 d,while the control group took diets containing PBS.At the 15th and 30th day after oral completion,serum was collected for non-specific immune parameters analysis.The results showed that at 15th day after oral completion,SOD,C3 and total protein of the fish feeding BSA-ISCOMs were significantly higher than those of the control fish(P<0.05),and interferon γ of the former was higher than that of the latter,but not significantly(P>0.05),while lysozyme of the former was significantly lower than that of the latter(P<0.05).At 30th day after oral completion,these parameters except lysozyme of the experimental group were not significantly different from the control group(P>0.05).The results of the challenge with Vibrio vulnificus at 30th day after oral completion showed that the cumulative mortality of the control and the experimental group was 100% and 26.7%,respectively,and the relative percent survival(RPS) was 73.3%.So this study indicated that BSA-ISCOMs can induce the immune response and improve the disease resistance of Pseudosciaena crocea.
- bovine serum albumin(BSA),
- immune stimulating complex(ISCOMs),
- Pseudosciaena crocea,
- non-specific immunity

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References(29)

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