Molecular Characteristics Analysis of D-strain Vaccine Virus of Muscovy Duck-origin Goose Parvovirus NS Protein Epitopes

WANG Shao; CHENG Xiao-xia; CHEN Shao-ying; ZHU Xiao-li; CHEN Shi-long; LIN Feng-qiang; LI Zhao-long

doi:10.19303/j.issn.1008-0384.2013.04.001

Volume 28 Issue 4

Apr. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(4): 301-308

WANG Shao, CHENG Xiao-xia, CHEN Shao-ying, ZHU Xiao-li, CHEN Shi-long, LIN Feng-qiang, LI Zhao-long. Molecular Characteristics Analysis of D-strain Vaccine Virus of Muscovy Duck-origin Goose Parvovirus NS Protein Epitopes[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 301-308. doi: 10.19303/j.issn.1008-0384.2013.04.001

Citation:

WANG Shao, CHENG Xiao-xia, CHEN Shao-ying, ZHU Xiao-li, CHEN Shi-long, LIN Feng-qiang, LI Zhao-long. Molecular Characteristics Analysis of D-strain Vaccine Virus of Muscovy Duck-origin Goose Parvovirus NS Protein Epitopes[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 301-308. doi: 10.19303/j.issn.1008-0384.2013.04.001

Citation:

WANG Shao, CHENG Xiao-xia, CHEN Shao-ying, ZHU Xiao-li, CHEN Shi-long, LIN Feng-qiang, LI Zhao-long. Molecular Characteristics Analysis of D-strain Vaccine Virus of Muscovy Duck-origin Goose Parvovirus NS Protein Epitopes[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 301-308. doi: 10.19303/j.issn.1008-0384.2013.04.001

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Molecular Characteristics Analysis of D-strain Vaccine Virus of Muscovy Duck-origin Goose Parvovirus NS Protein Epitopes

doi: 10.19303/j.issn.1008-0384.2013.04.001

1. Institute of Animal Husbandry and Veterinary Medicine,FuJian Academy of Agriculture Sciences,Fuzhou,Fujian 350013,China;
2. Fujian Animal Diseases Control Technology Development Center,Fuzhou,Fujian 350013,China

Received Date: 2013-03-11
Publish Date: 2013-04-18

Abstract

Abstract

To analyze the non-structural protein NS gene of vaccine muscovy duck (Cairina moschata) origin Goose parvovirus (GPV) D strain,apair of primers were designed by DNAStar software based on the published sequences of MDGPV-PT strain from GenBank. We examed the homology,heredity evolution,N-glycosylation sites,phosphorylation sites,B cell epitopes,T cell epitopes and their secondary structure of NS protein with bioinformatics software.The full length NS gene was amplified by PCR and cloned into pMD18-T vector. Sequencing demonstrated that the NS gene of MDGPV-D strain contained 1 884bps,encoding 627amino acids (GenBank number : JF926696) .The MDGPV-Dstrain NS gene was highly similar to that of MDPV (nt:97.9%98.6%,aa : 97.6% -98.2%) .It was also shown that there were 3 potential N-glycosylation sites and 27 phosphorylation sites existed in MDGPV-D strain NS protein,and 11Bcell epitopes,13CD8 + CTL epitopes and 10CD4 + Th epitopes as well.Then the prediction of secondary structure of NS protein showed that alpha helix,random coil and beta angle account for 40.67%,41.15% and 4.63% respectively.Comparison of the parental virulent strain PT with its attenuated vaccine strain D revealed two site-specific mutagenesis:one was in the nucleoside triphosphate (NTP) binding motif at amino acid 338,and the other in a CD4 + Th epitope at amino acid 225.These studies has provided a molecular basis for investigating attenuation mechanisms of MDGPV,and benefitted for further researches on genetically engineered MDGPV vaccines.
- MDGPV,
- NS protein,
- epitope,
- molecular characteristics

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References(15)

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