Metabonomic Heterogeneity Analysis for Pathogenicity of Ralstonia solanacearum

SHI Huai; LIU Bo; CHEN Zheng; ZHENG Xue-fang; PAN Zhi-zhen; CHEN Mei-chun; ZHU Yu-jing

doi:10.19303/j.issn.1008-0384.2013.04.009

Volume 28 Issue 4

Apr. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(4): 343-352

SHI Huai, LIU Bo, CHEN Zheng, ZHENG Xue-fang, PAN Zhi-zhen, CHEN Mei-chun, ZHU Yu-jing. Metabonomic Heterogeneity Analysis for Pathogenicity of Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 343-352. doi: 10.19303/j.issn.1008-0384.2013.04.009

Citation:

SHI Huai, LIU Bo, CHEN Zheng, ZHENG Xue-fang, PAN Zhi-zhen, CHEN Mei-chun, ZHU Yu-jing. Metabonomic Heterogeneity Analysis for Pathogenicity of Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 343-352. doi: 10.19303/j.issn.1008-0384.2013.04.009

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Metabonomic Heterogeneity Analysis for Pathogenicity of Ralstonia solanacearum

doi: 10.19303/j.issn.1008-0384.2013.04.009

Agricultural Bioresources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

Received Date: 2012-11-20
Publish Date: 2013-04-18

Abstract

Abstract

The metabolites of Ralstonia solanacearum with various pathogenic were analysed by metabonomic method based on LC/Q-TOF MS.Significant metabolic markers were used for classification pathogenic of Ralstonia solanacearum by PCA analysis,and PLS-DA prediction model were established.The result suggest that metabonomic analysis could be used for indentify virulent Ralstonia solanacearum strain and avirulent strain and Tn-5mutant strains.Furthermore,there were 12metabolites showed similar trend and had significant differences between virulent strain and avirulent strains(both wide-type and Tn-5mutant),suggesting that the 12metabolites are probably related to the pathogincity of Ralstonia solanacearum.
- metabonomic,
- ralstonia solanacearum,
- pathogenicity

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References(26)

References

[1]	刘波,朱育菁,林抗美,等.青枯雷尔氏菌在植株体内分布及其致病力的异质性研究[J].中国农业科学,2007,40(7):1559-1566.
[2]	BALABEL N M,EWEDA W E,MOSTAFA M I,et al.Someepidemiological aspects of Ralstonia solanacearum[J].Egyptian Journal of Agricultural Research,2005,83(4):1547-1564.
[3]	刘波,林营志,朱育菁,等.生防菌对青枯雷尔氏菌的致弱特性[J].农业生物技术学报,2004,12(3):322-329.
[4]	车建美,刘波,张彦,等.青枯雷尔氏菌致病性生物测定方法的研究[J].福建农业学报,2011,26(5):804-807.
[5]	刘波,蓝江林,车建美,等.青枯雷尔氏菌脂肪酸型与致病性的关系[J].中国农业科学,2009,42(2):511-522.
[6]	林娟,刘树滔,高珍娜,等.应用高效离子交换色谱分析青枯雷尔氏菌的致病力分化[J].应用与环境生物学报,2009,15(5):713-718.
[7]	宋浩,沙伟,丁伟,等.非标记定量蛋白质组学在烟草青枯病致病性研究中的应用[J].分子植物育种,2010,8(3):595-604.
[8]	VACLAVIK L,LACINA O,HAJSLOVA J,et al.The use ofhigh performance liquid chromatography-quadrupole time-of-flight mass spectrometry coupled to advanced data mining andchemometric tools for discrimination and classification of redwines according to their variety[J].Anal Chim Acta,2011,685:45-51.
[9]	史怀,刘波,陈峥,等.基于LC/Q-TOF MS的芽孢杆菌代谢组学分析方法[J].福建农业学报,2012,27(10):1112-1119.
[10]	WANG Q Z,WU C Y,CHEN T,et al.Integratingmetabolomics into a systems biology framework to exploitmetabolic complexity:Strategies and applications in microorganisms[J].Appl Microbiol Biotechnol,2006,70(2):151-161.
[11]	BUNDY J G,WILLEY T L,CASTELL R S,et al.Discrimination of pathogenic clinical isolates and laboratorystrains of Bacillus cereus by NMR-based metabolomicsprofiling[J].FEMS Microbiol Lett,2005,24,2(1):127-136.
[12]	BOURNE R,HIMMELREICH U,SHARMA A,et al.Identification of Enterococcus,Streptococcus,andStaphylococcus by multivariate analysis of proton magneticresonance spectroscopic data from plate cultures[J].J ClinMicrobiol,2001,39(8):2916-2923.
[13]	程本亮,车建美,刘波.青枯雷尔氏菌Tn5转座子无致病力突变株构建及其生物学特性[J].农业生物技术学报,2011,19(1):26-38.
[14]	BRINDLE J T,ANTTI H,HOLMES E,et al.Rapid andnoninvasive diagnosis of the presence and severity of coronaryheart disease using1 H NMR-based metabonomics[J].J NatMed,2002,8(12):1439-1442.
[15]	LINDON J C,NICHOLSON J K,HOLMES E,et al.Contemporary issues in toxicology the role of metabonomics intoxicology and its evaluation by the COMET project[J].Toxieol Appl Pharmacol,2003,187(3):137-146.
[16]	GERMAN J B,ROBERTS M A,WATLDNS S M.Personalmetabolomics as a next generation nutritional assessment[J].J Nutr,2003,133(12):4260-4266.
[17]	WIDODO,PATTERSON J H,NEWBIGIN E,et al.Metabolic responses to salt stress of barley(Hordeum vulgareL.)cultivars,Sahara and Clipper,which differ in salinitytolerance[J].Journal of Experimental Botany,2009,60:4089-4103.
[18]	FRSTER J,GOMBERT A K,NIELSEN J.A functionalgenomics approach using metabolomics and in silico pathwayanalysis[J].Biotechnol Bioeng,2002,79(7):730-712.
[19]	BOERSMA M G,SOLYANIKOVA I P,VANBERKEL W JH,et al.19 NMR metabolomics for the elucidation ofmicrobial degradation pathways of fluorophenols[J].J IndMicrobiol Biotechnol,2001,26(1/2):22-34.
[20]	BUCHHOLZ A,HURLEBAUS J,WANDERY C,et al.Metabolomics:quantification of intracellular metabolitedynamics[J].Biomol Eng,2002,19(1):5-15.
[21]	RAAMSDONK L M,TEUSINK B,BROADHURST D,et al.A functional genomics strategy that uses metabolomedata to reveal the phenotype of silent mutations[J].NatureBiotechnol,2001,19(1):45-50.
[22]	HOLMES E,WILSON I D,NICHOLSON J K.Metabolicphenotyping in health and disease[J].Cell,2008,134(5):714-717.
[23]	CHELL M A.Control of virulence and pathogenicity genes ofRalstonia solanacearum by an elaborate sensory network[J].Annu Rev Phytopathol,2000,38(9):263-292.
[24]	HORITA M,TSUCHIYA K,OOSHIRO A.Characteristicsof Ralstonia solanacearum biovar N2strains in Asia[J].Journal of Phytopathology,2005,153(4):209-213.
[25]	FLAVIER A B,GANOVA-RAEVA L M,SCHELL M A,et al.Hierarchical autoinduction in Ralstonia solanacearum:control of acyl-homoserine lactone production by a novelautoregulatory system responsive to 3-hydroxypalmitic acidmethyl ester[J].J Bacteriol,1997,179(22):7089-7097.
[26]	FLAVIER A B,SCHELL M A,DENNY T P.An RpoS(sigmaS)homologue regulates acylhomoserine lactone-dependent autoinduction in Ralstonia solanacearum[J].MolMicrobiol,1998,28(3):475-486.