Isolation and Molecular Identification of Southern Blight Pathogen in Anoectochilus roxburghii(Wall.)Lindl.

ZHAO Yun-qing; HUANG Ying-zhen; CHEN Jing-ying; LIU Bao-cai

doi:10.19303/j.issn.1008-0384.2013.04.011

Volume 28 Issue 4

Apr. 2013

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ZHAO Yun-qing, HUANG Ying-zhen, CHEN Jing-ying, LIU Bao-cai. Isolation and Molecular Identification of Southern Blight Pathogen in Anoectochilus roxburghii(Wall.)Lindl.[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 357-360. doi: 10.19303/j.issn.1008-0384.2013.04.011

Citation:

ZHAO Yun-qing, HUANG Ying-zhen, CHEN Jing-ying, LIU Bao-cai. Isolation and Molecular Identification of Southern Blight Pathogen in Anoectochilus roxburghii(Wall.)Lindl.[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 357-360. doi: 10.19303/j.issn.1008-0384.2013.04.011

Citation:

ZHAO Yun-qing, HUANG Ying-zhen, CHEN Jing-ying, LIU Bao-cai. Isolation and Molecular Identification of Southern Blight Pathogen in Anoectochilus roxburghii(Wall.)Lindl.[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 357-360. doi: 10.19303/j.issn.1008-0384.2013.04.011

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Isolation and Molecular Identification of Southern Blight Pathogen in Anoectochilus roxburghii(Wall.)Lindl.

doi: 10.19303/j.issn.1008-0384.2013.04.011

1. Institute of Agrobiological Resources,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;
2. Medicinal Plant Research Center,Fujian academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

Received Date: 2013-01-03
Publish Date: 2013-04-18

Abstract

Abstract

In this study,the suspected Pathogen of Anoectochilus roxburghii Southern blight was isolated by tissue separation and purified from samples with typical symptoms.Then the pathogen was identified by pathogenicity test,pathogen morphology and molecular biology methods.The results showed that the strain from southern blight infected Anoectochilus tissues collected from Fujian were isolated.Mycelium was white,velvet-like,close to the culture medium,radially extended to the surrounding containment and branches,colonies formed on the surface of sclerotia,sclerotia early white,then change to yellow,and finally was dark brown,spherical or irregular spherical,smooth and shiny surface.The isolated strain has pathogenicity to tested Anoectochilus.The pathogen was identified pathogenically,morphologically and molecularly.Based on these data,it can be confirmed that the pathogen was Sclerotium rolfsii Sacc.
- Anoectochilus roxburghii(Wall.)Lindl.,
- southern blight,
- pathogens,
- identification

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References(19)

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