Comparative evaluation on different methods to extract endophytic bacteria DNA from Diaphorina citri Kuwayama

YANG Jin-xia; RUAN Chuan-qing; LIU Bo; CHEN Jian-li

doi:10.19303/j.issn.1008-0384.2013.04.012

Volume 28 Issue 4

Apr. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(4): 361-365

YANG Jin-xia, RUAN Chuan-qing, LIU Bo, CHEN Jian-li. Comparative evaluation on different methods to extract endophytic bacteria DNA from Diaphorina citri Kuwayama[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 361-365. doi: 10.19303/j.issn.1008-0384.2013.04.012

Citation:

YANG Jin-xia, RUAN Chuan-qing, LIU Bo, CHEN Jian-li. Comparative evaluation on different methods to extract endophytic bacteria DNA from Diaphorina citri Kuwayama[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 361-365. doi: 10.19303/j.issn.1008-0384.2013.04.012

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Comparative evaluation on different methods to extract endophytic bacteria DNA from Diaphorina citri Kuwayama

doi: 10.19303/j.issn.1008-0384.2013.04.012

1. College of Life Science,Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China;
2. Bio-Resources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

Received Date: 2013-01-10
Publish Date: 2013-04-18

Abstract

Abstract

To find a better method for the extraction of endophytic bacteria DNA from Asian Citrus Psyllid,Diaphorina citri Kuwayama,we compared the traditional methods and FastDNA Kits.The result showed that the DNA template extracted with the method of FastDNA Kits(MP Biomedical)was more suitable for PCR amplification,and the amplification product could be well used for cloning and sequencing.In the contrast,although the other three methods were cheaper,the amount and purity of DNA extracted with them were decreased.Our results suggested that the extraction with the FastDNA kits was stable and convenient for operation and standardization but expensiveness.In summary,four methods had respective advantages and disadvantages,and FastDNA kits provided more stable DNA for PCR and sequence cloning.
- Diaphorina citri Kuwayama,
- endophytic bacteria,
- total DNA,
- extraction

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References(14)

References

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