Cloning and Sequence Analysis on Gene of Chlorophyll a/b-binding Protein from Narcissustazetta var. chinensis

LIN Jiang-bo; WANG Wei-ying; ZOU Hui; DAI Yi-min

doi:10.19303/j.issn.1008-0384.2013.05.011

Volume 28 Issue 5

May 2013

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LIN Jiang-bo, WANG Wei-ying, ZOU Hui, DAI Yi-min. Cloning and Sequence Analysis on Gene of Chlorophyll a/b-binding Protein from Narcissustazetta var. chinensis[J]. Fujian Journal of Agricultural Sciences, 2013, 28(5): 463-467. doi: 10.19303/j.issn.1008-0384.2013.05.011

Citation:

LIN Jiang-bo, WANG Wei-ying, ZOU Hui, DAI Yi-min. Cloning and Sequence Analysis on Gene of Chlorophyll a/b-binding Protein from Narcissustazetta var. chinensis[J]. Fujian Journal of Agricultural Sciences, 2013, 28(5): 463-467. doi: 10.19303/j.issn.1008-0384.2013.05.011

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Cloning and Sequence Analysis on Gene of Chlorophyll a/b-binding Protein from Narcissustazetta var. chinensis

doi: 10.19303/j.issn.1008-0384.2013.05.011

Sugarcane Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Fujian 363005,China

Received Date: 2013-04-15
Publish Date: 2013-05-18

Abstract

Abstract

From a cDNA fragment of the suppression subtractive hybridization of Narcissus tazetta var.chinensis induced by paclobutrazol,the gene of the light-harvesting chlorophyll a/b-binding protein,Ntcab1,was isolated and cloned by RACE and RT-PCR.The full length of Ntcab1cDNA was determined to be 1 012bp with a 798bp ORF,which encoded a protein of 265 amino acids with a predicted molecular weight of 28.155kD and a theoretical isoelectric point of 5.48.The phylogenetic analysis showed that Ntcab1could be clustered together with LHCb1.1 from Arabidopsis thaliana,and had a close relationship with those in Ophiopogon japonicus(ACD64978.1)and Lycoris radiata(ADC35579.1).The semi-quantitative RT-PCR revealed that the expression of Ntcab1was higher during sprouting and early flowering after it was sprayed with paclobutrazol than water.
- Narcissus tazetta var. Chinensis,
- light-harvesting chlorophyll a/b-binding protein,
- gene cloning

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References(14)

References

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