Cloning and Sequence Analysis Phosphofructokinase Gene from Aspergillus oryzae

ZHANG Ka; DU Yu; CHEN Hong-wen

doi:10.19303/j.issn.1008-0384.2013.06.016

Volume 28 Issue 6

Jun. 2013

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ZHANG Ka, DU Yu, CHEN Hong-wen. Cloning and Sequence Analysis Phosphofructokinase Gene from Aspergillus oryzae[J]. Fujian Journal of Agricultural Sciences, 2013, 28(6): 584-588. doi: 10.19303/j.issn.1008-0384.2013.06.016

Citation:

ZHANG Ka, DU Yu, CHEN Hong-wen. Cloning and Sequence Analysis Phosphofructokinase Gene from Aspergillus oryzae[J]. Fujian Journal of Agricultural Sciences, 2013, 28(6): 584-588. doi: 10.19303/j.issn.1008-0384.2013.06.016

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Cloning and Sequence Analysis Phosphofructokinase Gene from Aspergillus oryzae

doi: 10.19303/j.issn.1008-0384.2013.06.016

Department of Bioengineering and Biotechnology,College of Chemical Engineering,Huaqiao University,Xiamen,Fujian 361021,China

Received Date: 2013-04-17
Publish Date: 2013-06-18

Abstract

Abstract

Phosphofructokinase(PFK)is one of the important regulatory enzymes in the Embden Meyerhof Parnas pathway.The PFK gene(pfkA)was cloned from the wine-brewing Aspergillus oryzae,CICC2012.It was subsequently sequenced and submitted to Genbank(accession number:KC113503.1)for verification.The sequence analysis revealed that pfkA was 2,722bp in length containing two introns,and 2,358bp long with an open reading frame encoding 785amino acids.The PFK was a hydrophilic protein containing two family signatures with a molecular weight of 86.0kD and an isoelectric point of 6.38.Its secondary structure consisted of 42.68% α-helix,14.27%β-folding and 43.06% random coil.The 3Dhomology model showed two(i.e.,N-and C-terminal) domains with the substrate,fructose-6-phosphate,bound to the N-terminal domain.The phylogenetic tree indicated pfkA′s close relationship with PFKs from other filamentous fungi.
- phosphofructokinase,
- Aspergillus oryzae,
- cloning,
- sequence analysis

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References(20)

References

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