Seed Purity Identification of Bitter Gourd Hybrid by RAPD Markers

ZHANG Shao-ping; ZHANG Yu-can; ZHANG Wei-guang; LAI Zheng-feng; WU Shui-jin

doi:10.19303/j.issn.1008-0384.2013.08.020

Volume 28 Issue 8

Aug. 2013

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(8): 828-831

ZHANG Shao-ping, ZHANG Yu-can, ZHANG Wei-guang, LAI Zheng-feng, WU Shui-jin. Seed Purity Identification of Bitter Gourd Hybrid by RAPD Markers[J]. Fujian Journal of Agricultural Sciences, 2013, 28(8): 828-831. doi: 10.19303/j.issn.1008-0384.2013.08.020

Citation:

ZHANG Shao-ping, ZHANG Yu-can, ZHANG Wei-guang, LAI Zheng-feng, WU Shui-jin. Seed Purity Identification of Bitter Gourd Hybrid by RAPD Markers[J]. Fujian Journal of Agricultural Sciences, 2013, 28(8): 828-831. doi: 10.19303/j.issn.1008-0384.2013.08.020

Citation:

PDF( 1250 KB)

Seed Purity Identification of Bitter Gourd Hybrid by RAPD Markers

doi: 10.19303/j.issn.1008-0384.2013.08.020

1. Fujian-Taiwan Horticure Research Center,Fujian Academy of Agricultural Sciences;
2. Institute of Agriculture and Biological Resources,Fujian Academy of Agricultural Sciences;
3. Fujian Academy of Agricultural Sciences

Received Date: 2013-05-12
Publish Date: 2013-08-18

Abstract

Abstract

The DNA fingerprints of xincui bitter gourd and its parents were analyzed by RAPD to obtain the genes differentially expressed.1out of the 120screened primers could amplify the characteristic polymorphic bands of F1generation with its female parent after repeated experiments.With the only one primer possessing high stability and repeatability, 6000individuals of Xincui bitter gourd from Hexi Corridor in Gansu province were identified, and our results ensured the promotion to Xincui bitter gourd, effectively.
- bitter gourd,
- hybrid,
- purity,
- RAPD

FullText(HTML)

References(9)

References

[1]	熊丽曼, 王贤磊, 高兴旺, 等.应用SRAP标记鉴定甜瓜种子纯度[J].种子, 2012, 31 (2) :9-12.
[2]	张菊平, 张兴志, 张长远.碧绿3号苦瓜种子纯度的RAPD检测研究[J].种子, 2004, (23) :25-26.
[3]	林珲, 李永平, 朱海生, 等.应用RAPD标记鉴定苦瓜杂种纯度[J].福建农业学报, 2011, 26 (6) :977-980.
[4]	DEY S S, SINGH A K, CHANDEL D, et al.Genetic diversity of bitter gourd (Momordica L.) genotypes revealed by RAPD markers and Agronomic traits[J].Sci Hort, 2006, 109:21-28.
[5]	张长远, 孙妮, 胡开林.苦瓜品种亲缘关系的RAPD分析[J].分子植物育种, 2005, 3, (4) :515-519.
[6]	高山, 林碧英, 许端祥, 等.苦瓜种质遗传多样性的RAPD和ISSR分析[J].植物遗传资源学报, 2010, 11 (1) :78-83.
[7]	温庆放, 李大忠, 朱海生, 等.不同来源苦瓜亲缘关系RAPD分析[J].福建农业学报, 2005, 20 (3) :185-188.
[8]	BEHERA T K, SINGH A K, STAUB J E, et al.Comparative analysis of geneticdiversity in Indian bitter gourd (Momordica charantia L.) using RAPD and ISSR markers for developing crop improvement strategies[J].Sci Hort, 2008, 115:209-217.
[9]	HULYA.RAPD markers assisted varietal identification and genetic purity in pepper Capsicum annuum[J].Scientia Horticulture, 2003, 97:211-218.