Rapid Propagation Technology for Pendent Cymbidiumand Generation of Aseptic Seedling

ZHOU Hui-ming; LIN Hui-feng; SHANG Wei; LIN Fa-zhuang; WANG Xue-feng; PAN Da-ren

doi:10.19303/j.issn.1008-0384.2013.10.010

Volume 28 Issue 10

Oct. 2013

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ZHOU Hui-ming, LIN Hui-feng, SHANG Wei, LIN Fa-zhuang, WANG Xue-feng, PAN Da-ren. Rapid Propagation Technology for Pendent Cymbidiumand Generation of Aseptic Seedling[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 981-986. doi: 10.19303/j.issn.1008-0384.2013.10.010

Citation:

ZHOU Hui-ming, LIN Hui-feng, SHANG Wei, LIN Fa-zhuang, WANG Xue-feng, PAN Da-ren. Rapid Propagation Technology for Pendent Cymbidiumand Generation of Aseptic Seedling[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 981-986. doi: 10.19303/j.issn.1008-0384.2013.10.010

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Rapid Propagation Technology for Pendent Cymbidiumand Generation of Aseptic Seedling

doi: 10.19303/j.issn.1008-0384.2013.10.010

1. Sanming Academy of Agricultural Science;
2. Fujian Agriculture and Forestry University

Received Date: 2013-06-13
Publish Date: 2013-10-18

Abstract

Abstract

To established a system of aseptic sowing and rapid propagation technology for pendent Cymbidium, the effects of basic culture media, hormone, organic additive and AC on pendent Cymbidium seeds germination, multiplication, differentiation and rooting was investigated by applying the seed→protocorm→the whole plant model.Our results showed that 90seeds germinated and developed into protocorm in 1/2MS medium containing 1.0 mg·L-16-BA+1.0 mg·L-1 NAA+0.5g·L-1 AC+20g·L-1 sugar after 60d.The best multiplication of protocorm (multiplication coefficient 6.8) was reached in 1/2MS medium containing 1.0 mg·L-1 6-BA+0.5 mg·L-1 NAA+1.0 mg·L-1 KT+0.5g·L-1 AC+20g·L-1 sugar.Meanwhile, the best differentiation (differentiation rate 89.2%) of protocorm was reached in 1/2MS medium containing 0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+0.5g·L-1 AC+20g·L-1sugar.Furthermore, the best rooting (rooting rate 94.5%, plant length up to 5.6cm) was reached in 1/2MS medium containing 1.0mg·L-1 NAA +50g·L-1 mashed potato+0.5 g·L-1 AC+20g·L-1 sugar after 30d.
- pendent Cymbidium,
- aseptic sowing,
- protocorm

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References(12)

References

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