Development of CLIA Analyzing Kit for Clenbuterol

SHEN Hao-long; ZHANG Zhang-gong; CHEN Qiao-qin; JIANG Liang-zhen; ZHUANG Xiao-yong; HUANG Jiang-shan; GUO Shu-lin; HUANG Yin-yao

doi:10.19303/j.issn.1008-0384.2013.10.021

Volume 28 Issue 10

Oct. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(10): 1039-1044

SHEN Hao-long, ZHANG Zhang-gong, CHEN Qiao-qin, JIANG Liang-zhen, ZHUANG Xiao-yong, HUANG Jiang-shan, GUO Shu-lin, HUANG Yin-yao. Development of CLIA Analyzing Kit for Clenbuterol[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 1039-1044. doi: 10.19303/j.issn.1008-0384.2013.10.021

Citation:

SHEN Hao-long, ZHANG Zhang-gong, CHEN Qiao-qin, JIANG Liang-zhen, ZHUANG Xiao-yong, HUANG Jiang-shan, GUO Shu-lin, HUANG Yin-yao. Development of CLIA Analyzing Kit for Clenbuterol[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 1039-1044. doi: 10.19303/j.issn.1008-0384.2013.10.021

Citation:

SHEN Hao-long, ZHANG Zhang-gong, CHEN Qiao-qin, JIANG Liang-zhen, ZHUANG Xiao-yong, HUANG Jiang-shan, GUO Shu-lin, HUANG Yin-yao. Development of CLIA Analyzing Kit for Clenbuterol[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 1039-1044. doi: 10.19303/j.issn.1008-0384.2013.10.021

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Development of CLIA Analyzing Kit for Clenbuterol

doi: 10.19303/j.issn.1008-0384.2013.10.021

1. Xiamen Bosheng Biology Int.;
2. The First Hospital of Xiamen City;
3. Xiamen Entry-Exit Inspection and Quarantine Bureau of the People′s Republic of China

Received Date: 2013-02-11
Publish Date: 2013-10-18

Abstract

Abstract

A highly active antibody was produced by making manmade antigen of Clenbuterol.We established a direct competition method to detect Clenbuterol that used HRP as markers and luminol as chemiluminescent substrates, with which many parameters were optimized, including coating buffer, coating condition, blocking condition, incubating time, and enzyme consumption.Based on this method we developed a chemiluminescent detecting kit, whose linear range was 0.1-8.1ng·mL-1, the linear correlation coefficient was r=0.996, the IC50 was 0.607ng mL-1, the detection limit was under 0.1ng·mL-1, the within-run assay coefficient of variation was 6.76%-8.31%, the between-run assay coefficient of variation was 7.4%, the coefficient of recovery was between (90±15) %, and there was no cross reaction with otherβ-agonists.
- clenbuterol,
- CLIA,
- kit

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References(6)

References

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