Prokaryotic Expression of E Protein from Duck Tembusu Virus

SHI Shao-hua; CHENG Long-fei; FU Guang-hua; CHEN Hong-mei; WAN Chun-he; FU Qiu-ling; LIN Jian-sheng; HUANG Yu

doi:10.19303/j.issn.1008-0384.2014.10.001

Volume 29 Issue 10

Oct. 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(10): 935-938

SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, FU Qiu-ling, LIN Jian-sheng, HUANG Yu. Prokaryotic Expression of E Protein from Duck Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(10): 935-938. doi: 10.19303/j.issn.1008-0384.2014.10.001

Citation:

SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, FU Qiu-ling, LIN Jian-sheng, HUANG Yu. Prokaryotic Expression of E Protein from Duck Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(10): 935-938. doi: 10.19303/j.issn.1008-0384.2014.10.001

Citation:

SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, FU Qiu-ling, LIN Jian-sheng, HUANG Yu. Prokaryotic Expression of E Protein from Duck Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(10): 935-938. doi: 10.19303/j.issn.1008-0384.2014.10.001

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Prokaryotic Expression of E Protein from Duck Tembusu Virus

doi: 10.19303/j.issn.1008-0384.2014.10.001

1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences;
2. Fujian Animal Diseases Control Technology Development Center

Received Date: 2014-04-10
Publish Date: 2014-10-18

Abstract

Abstract

The entire E gene from the duck Tembusu virus (strain WR) was amplified by RT-PCR, and cloned into the pEASY-T3 vector.The recombinant plasmids carrying the target fragment were digested with Bam HI and Xho I, and cloned into the pET-32 avector to construct recombinant plasmid to be named pET-E.pET-E was subsequently transformed into Escherichia coli BL21 (DE3) .The fusion protein was expressed by IPTG induction, and presented mainly as inclusion bodies.The positive western blotting result suggested a strong reactinogenicity of the protein.
- duck tembusu virus,
- E gene,
- prokaryotic expression

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References(17)

References

[1]	KONO Y, TSUKAMOTO K, ABD HAMID M, et al.Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus[J].Am J Trop Med Hyg, 2000, 63 (1-2) :94-101.
[2]	曹贞贞, 张存, 黄瑜, 等.鸭出血性卵巢炎的初步研究[J].中国兽医杂志, 2010, 46 (12) :3-6.
[3]	万春和, 施少华, 程龙飞, 等.一种引起种 (蛋) 鸭产蛋骤降新病毒的分离与初步鉴定[J].福建农业学报, 2010, 25 (6) :663-666.
[4]	SU J, LI S, HU X, et al.Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related Flavivirus[J].PLoS One, 2011, 6 (3) :e18106.
[5]	CAO Z, ZHANG C, LIU Y, et al.Tembusu virus in ducks, China[J].Emerg Infect Dis, 2011, 17 (10) :1873-1875.
[6]	傅光华, 黄瑜, 施少华, 等.鸡黄病毒的分离与初步鉴定[J].福建畜牧兽医, 2011, 33 (3) :1-2.
[7]	陈仕龙, 陈少莺, 王劭, 等.一种引起蛋鸡产蛋下降的新型黄病毒的分离与初步鉴定[J].福建农业学报, 2011, 26 (2) :170-174.
[8]	黄欣梅, 李银, 赵冬敏, 等.新型鹅黄病毒JS804毒株的分离与鉴定[J].江苏农业学报, 2011, 27 (2) :354-360.
[9]	施少华, 傅光华, 万春和, 等.鹅源坦布苏病毒的分离及初步鉴定[J].中国兽医杂志, 2012, 48 (12) :10-11.
[10]	MIYATA T, TAFUKU S, HARAKUNI T, et al.A bionanocapsule containing envelope protein domain III of Japanese encephalitis virus protects mice against lethal Japanese encephalitis virus infection[J].Microbiol Immunol, 2013, 57 (6) :470-477.
[11]	SAXENA D, PARIDA M, RAO P V, et al.Cloning and expression of an envelope gene of West Nile virus and evaluation of the protein for use in an IgM ELISA[J].Diagn Microbiol Infect Dis, 2013, 75 (4) :396-401.
[12]	张琳, 逯茂洋, 胡北侠, 等.4株鸭坦布苏病毒包膜蛋白基因的分子进化分析及表达[J].中国兽医学报, 2013, 33 (2) :175-180.
[13]	黄欣梅, 李银, 赵冬敏, 等.鹅黄病毒囊膜蛋白的原核表达及抗原性分析[J].华北农学报, 2012, 27 (5) :33-37.
[14]	姬希文, 李雪松, 边延峰, 等.鸭坦布苏病毒E蛋白的原核表达[J].中国动物传染病学报, 2012, 20 (2) :42-46.
[15]	刘宗梁, 付钰广, 吉艳红, 等.鸭坦布苏病毒E蛋白膜外区的原核表达与鉴定[J].中国兽医科学, 2013, 43 (8) :850-854.
[16]	柴顺秀, 马花, 韩英, 等.鸭坦布苏病毒E蛋白主要抗原区域的原核表达与鉴定[J].动物医学进展, 2013, 34 (7) :49-53.
[17]	杨少艳, 于可响, 王华, 等.鸭坦布苏病毒E基因的克隆表达及初步应用[J].浙江农业学报, 2012, 24 (5) :782-786.