ISSR Analysis on Genetic Relationship among 29 Oncidium Germplasms

LUO Yuan-hua; ZHONG Huai-qin; HUANG Min-ling; WU Jian-she

doi:10.19303/j.issn.1008-0384.2014.11.007

Volume 29 Issue 11

Nov. 2014

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LUO Yuan-hua, ZHONG Huai-qin, HUANG Min-ling, WU Jian-she. ISSR Analysis on Genetic Relationship among 29 Oncidium Germplasms[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1079-1082. doi: 10.19303/j.issn.1008-0384.2014.11.007

Citation:

LUO Yuan-hua, ZHONG Huai-qin, HUANG Min-ling, WU Jian-she. ISSR Analysis on Genetic Relationship among 29 Oncidium Germplasms[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1079-1082. doi: 10.19303/j.issn.1008-0384.2014.11.007

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ISSR Analysis on Genetic Relationship among 29 Oncidium Germplasms

doi: 10.19303/j.issn.1008-0384.2014.11.007

1. Crop Research Institute,Fujian Academy of Agricultural Sciences;
2. Flower Research Center,Fujian Academy of Agricultural Sciences;
3. Fujian Engineering Research Center for Characteristic Floriculture

Received Date: 2014-07-14
Publish Date: 2014-11-18

Abstract

Abstract

Based on the ISSR molecular markers, agenetic comparison among 29 Oncidium germplasms was established.The 10 ISSR primers produced 144 DNA bands, averaging 14.4DNA bands per primer, and of which124 (86.11%) were polymorphic.The genetic similarity coefficients of the germplasms ranged from 0.34 to 0.91.At the similarity coefficient of 0.56, the 29 germplasm accessions were classified into 3groups.The clustering results were basically consistent with the traditional classification, which is closely correlated to the flower phenotype of germplasm resources.The result obtained from this study seemed to preliminarily validate the application of ISSR molecular markers for genetic analysis on Oncidium.
- Oncidium,
- genetic relationship,
- ISSR

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References(12)

References

[1]	陈和明, 朱根发, 操君喜, 等.15份文心兰种质资源生物学特性观察[J].广东农业科学, 2011, (7) :71-73.
[2]	柯海丽, 黎维诗, 刘冬梅, 等.文心兰切花品种的栽培比较研究[J].安徽农业科学, 2012, 40 (34) :16556-16557 , 16559.
[3]	孙映波, 尤毅, 朱根发, 等.施用不同肥料对‘黄金2号’文心兰叶片酶活性的影响[J].热带作物学报, 2013, 34 (4) :621-624.
[4]	杨光穗, 黄素荣, 黄少华, 等.4种文心兰的核型分析[J].热带作物学报, 2013, 34 (1) :014-017.
[5]	罗远华, 莫饶, 蔡林宏, 等.文心兰品种变异RAPD分子检测技术的建立及应用[J].热带农业科学, 2009, 29 (7) :28-31.
[6]	崔广荣, 张子学, 张从宇, 等.文心兰EMS离体诱变及再生苗RAPD检测[J].热带作物学报, 2011, 32 (2) :261-268.
[7]	崔广荣, 张子学, 张从宇, 等.文心兰NaN3离体化学诱变及RAPD检测[J].广西植物, 2011, 31 (6) :836-843.
[8]	周艳霞, 尹俊梅, 任羽, 等.文心兰种质遗传多样性的SRAP分析[J].热带农业科学, 2009, 29 (7) :43-46.
[9]	严华, 张冬梅, 罗玉兰, 等.38种国兰亲缘关系的ISSR分析[J].分子植物育种, 2010, 8 (4) :736-741.
[10]	谢启鑫, 缪南生, 宋小民, 等.蝴蝶兰种质资源遗传多样性的ISSR分析[J].西北植物学报, 2010, 30 (7) :1331-1336.
[11]	卢家仕, 卜朝阳, 吕维莉, 等.20份兰科植物的ISSR遗传多样性分析[J].西南农业学报, 2012, 25 (6) :2252-2257.
[12]	CHEN W H, TSENG I C, TSAI W C, et al.AFLP fingerprinting and conversion to sequence-tag Site markers for the identification of Oncidium cultivars[J].Journal of Horticultural Science&Biotechnology, 2006, 81 (5) :791-796.